pAAVLINKv1-pCALM1-Cre-3'-DOCK1-FLAG

LOCUS       pAAVLINKv1-pCALM1-Cre-3'-DOCK1-FLAG                6722 bp DNA     circular SYN 18-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6722)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported May 18, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6722
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     promoter        145..265
                     /label=pCALM1
     CDS             277..1308
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     intron          1309..1349
                     /label=Splicing Donor
     protein_bind    1356..1389
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1390..1481
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1482..3423
                     /codon_start=1
                     /label=DOCK1
                     /translation="VFVQAL*PAQGV**LHRSGLHLASPCKAS*VVGGCVCGPPHPAGR
                     VPGHHAGTAEGAALPGNHPLLRQRQDVGGGHCLGQGASRAV*ERNV*L*ATQRIAEKTG
                     SVL*KHRQSDQAQA*LFCCWLLRTRVPHIPAGKSFHLPRERV*APGRF*GSALNSVSKR
                     RENEDNISTRRRY*KLSWPVYSVLHSEAQTRSAS*VSQASVRADCKFLQGERGPAI*IF
                     SANPEGREKPRQ*ICEYVDRENHIYNCI*ITWNFKVV*GQVCFHGGNQPPGECHRDHAA
                     DERQDQQHGAAAPG*PQPAHQPALHAPERHRGPSCHGGLRKLRKGLLYRPVPAGAP*GP
                     *KDREAQGPDCLADSFSGRRDQNPWRQSHGGTEAVPREDGGLFQTAEGKGGERVRRPNH
                     ALKSG**KRQPPPVHGAVLHDAFLIPPSVCGLCLFPLIGQHPFQTRLRRVCPGASPAKE
                     NALQVPGQAGQG*PGEGEEGQEEGKKEQQTSRDI*ERI*THRHFPAAV*GCDPFGNDKS
                     PAAPETEEPGDERHWKRKALLGVPLVTVLPANTPSSYTKGQAQLQHAVELGAERHDGDG
                     RGRCPTPSASQRQRGRLREFDGKPGLAGLANTSTSPSTPEASATSTAQQNSASSPSKDN
                     SQADIGGLWDRA"
     misc_feature    1700..1705
                     /label=SDS
     misc_feature    2068..2073
                     /label=SDS
     misc_feature    3364..3369
                     /label=SDS
     CDS             3430..3495
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3511..3942
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3655..3660
                     /label=SDS
     repeat_region   3985..4125
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    3985..4114
                     /label=AAV2 ITR
     rep_origin      4200..4655
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4937..5041
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5042..5902
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6073..6661
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
      181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
      241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
      301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
      361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
      421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
      481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
      541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
      601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
      661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
      721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
      781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
      841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
      901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
      961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
     1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
     1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
     1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
     1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
     1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
     1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
     1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
     1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtatttgta caagctctgt
     1501 gacctgcaca aggagtgtga taactacacc gaagcggctt acaccttgct tctccatgca
     1561 aagcttctta agtggtcgga ggatgtgtgt gtggcccacc tcacccagcg ggacgggtac
     1621 caggccacca cgcagggaca gctgaaggag cagctctacc aggaaatcat ccactacttc
     1681 gacaaaggca agatgtggga ggaggccatt gccttgggca aggagctagc cgagcagtat
     1741 gagaacgaaa tgtttgatta tgagcaactc agcgaattgc tgaaaaaaca ggctcagttt
     1801 tatgaaaaca tcgtcaaagt gatcaggccc aagcctgact attttgctgt tggctactac
     1861 ggacaagggt tccccacatt cctgcgggga aaagttttca tttaccgagg gaaagagtat
     1921 gagcgccggg aagattttga ggctcggctc ttaactcagt ttccaaacgc cgagaaaatg
     1981 aagacaacat ctccaccagg cgacgatatt aaaaactctc ctggccagta tattcagtgc
     2041 ttcacagtga agcccaaact cgatctgcct cctaagtttc acaggccagt gtcagagcag
     2101 attgtaagtt tttacagggt gaacgaggtc cagcgatttg aatattctcg gccaatccgg
     2161 aagggagaga aaaacccaga caatgaattt gcgaatatgt ggatcgagag aaccatatat
     2221 acaactgcat ataaattacc tggaatttta aggtggtttg aggtcaagtc tgttttcatg
     2281 gtggaaatca gccccctgga gaatgccatc gagaccatgc agctgacgaa cgacaagatc
     2341 aacagcatgg tgcagcagca cctggatgac cccagcctgc ccatcaaccc gctctccatg
     2401 ctcctgaacg gcatcgtgga cccagctgtc atggggggct tcgcaaacta cgaaaaggcc
     2461 ttctttacag accggtacct gcaggagcac cctgaggccc atgaaaagat cgagaagctc
     2521 aaggacctga ttgcttggca gattcctttt ctggccgaag ggatcagaat ccatggagac
     2581 aaagtcacgg aggcactgag gccgttccac gagaggatgg aggcctgttt caaacagctg
     2641 aaggaaaagg tggagaaaga gtacggcgtc cgaatcatgc cctcaagtct ggatgataga
     2701 agaggcagcc gcccccggtc catggtgcgg tccttcacga tgccttcctc atcccgccct
     2761 ctgtctgtgg cctctgtctc ttccctctca tcggacagca ccccttccag accaggctcc
     2821 gacgggtttg ccctggagcc tctcctgcca aagaaaatgc actccaggtc ccaggacaag
     2881 ctggacaagg atgacctgga gaaggagaag aaggacaaga agaaggaaaa aaggaacagc
     2941 aaacatcaag agatatttga gaaagaattt aaacccaccg acatttccct gcagcagtct
     3001 gaggctgtga tcctttcgga aacgataagt cccctgcggc cccagagacc gaagagccag
     3061 gtgatgaacg tcattggaag cgaaaggcgc ttctcggtgt ccccctcgtc accgtcctcc
     3121 cagcaaacac cccctccagt tacaccaagg gccaagctca gcttcagcat gcagtcgagc
     3181 ttggagctga acggcatgac ggggacggac gtggccgatg tcccaccccc tctgcctctc
     3241 aaaggcagcg tggcagatta cgggaatttg atggaaaacc aggacttgct gggctcgcca
     3301 acacctccac ctccccctcc acaccagagg catctgccac ctccactgcc cagcaaaact
     3361 ccgcctcctc cccctccaaa gacaactcgc aagcagacat cggtggactc tgggatcgtg
     3421 cagtgtacag actacaaaga ccatgacggt gattataaag atcatgacat cgactacaag
     3481 gatgacgatg acaagtaaga attctgatga ctcgagataa tcaacctctg gattacaaaa
     3541 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     3601 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     3661 tgtataaatc ctggttagtt cttgccacgg cggaactcat cgccgcctgc cttgcccgct
     3721 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgtttatt tgtgaaattt
     3781 gtgatgctat tgctttattt gtaaccatct agctttattt gtgaaatttg tgatgctatt
     3841 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     3901 tttatgtttc aggttcaggg ggagatgtgg gaggtttttt aaagatctgg agaccggtgt
     3961 ggcacgtgcg gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc
     4021 tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg
     4081 cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt
     4141 attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta
     4201 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     4261 tacacttgcc agcgccttag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     4321 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     4381 tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc
     4441 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     4501 actcttgttc caaactggaa caacactcaa ctctatctcg ggctattctt ttgatttata
     4561 agggattttg ccgatttcgg tctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     4621 cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg
     4681 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     4741 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     4801 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     4861 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     4921 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     4981 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     5041 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     5101 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     5161 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     5221 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     5281 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     5341 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     5401 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     5461 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     5521 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     5581 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     5641 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     5701 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     5761 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     5821 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     5881 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     5941 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     6001 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     6061 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     6121 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     6181 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     6241 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     6301 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     6361 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     6421 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     6481 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     6541 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     6601 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6661 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     6721 gt
//