| 质粒名称: | pAAVLINK-EF1a-HA-5'-PTCH1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001638 |
| 产品用途: | 5' AAVLINK plasmid for PTCH1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | PTCH1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-PTCH1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-PTCH1载体质粒基本信息
pAAVLINK-EF1a-HA-5'-PTCH1质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-PTCH1载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-PTCH1质粒载体简介
pAAVLINK-EF1a-HA-5'-PTCH1质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-PTCH1 5858 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5858)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 22, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5858
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..2998
/codon_start=1
/label=PTCH1
/translation="MASAGNAAEPQDRGGGGSGCIGAPGRPAGGGRRRRTGGLRRAAAP
DRDYLHRPSYCDAAFALEQISKGKATGRKAPLWLRAKFQRLLFKLGCYIQKNCGKFLVV
GLLIFGAFAVGLKAANLETNVEELWVEVGGRVSRELNYTRQKIGEEAMFNPQLMIQTPK
EEGANVLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEY
LYPCLIITPLDCFWEGAKLQSGTAYLLGKPPLRWTNFDPLEFLEELKKINYQVDSWEEM
LNKAEVGHGYMDRPCLNPADPDCPATAPNKNSTKPLDMALVLNGGCHGLSRKYMHWQEE
LIVGGTVKNSTGKLVSAHALQTMFQLMTPKQMYEHFKGYEYVSHINWNEDKAAAILEAW
QRTYVEVVHQSVAQNSTQKVLSFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTML
RWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFL
LAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQ
AAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTDTHD
NTRYSPPPPYSSHSFAHETQITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPVTVTQ
DTLSCQSPESTSSTRDLLSQFSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLKPKAK"
misc_feature 1063..1068
/label=SDS
misc_feature 1489..1494
/label=SDS
misc_feature 1502..1507
/label=SDS
misc_feature 1761..1766
/label=SDS
intron 2999..3039
/label=Splicing Donor
misc_feature 3043..3076
/label=lox2272-JT1571
repeat_region 3121..3261
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3121..3250
/label=AAV2 ITR
rep_origin 3336..3791
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4073..4177
/gene="bla"
/label=AmpR promoter
CDS 4178..5038
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5209..5797
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctat ggcctcggct ggtaacgccg ccgagcccca
781 ggaccgcggc ggcggcggca gcggctgtat cggtgccccg ggacggccgg ctggaggcgg
841 gaggcgcaga cggacggggg ggctgcgccg tgctgccgcg ccggaccggg actatctgca
901 ccggcccagc tactgcgacg ccgccttcgc tctggagcag atttccaagg ggaaggctac
961 tggccggaaa gcgccgctgt ggctgagagc gaagtttcag agactcttat ttaaactggg
1021 ttgttacatt caaaaaaact gcggcaagtt cttggttgtg ggcctcctca tatttggggc
1081 cttcgcggtg ggattaaaag cagcgaacct cgagaccaac gtggaggagc tgtgggtgga
1141 agttggagga cgagtaagtc gtgaattaaa ttatactcgc cagaagattg gagaagaggc
1201 tatgtttaat cctcaactca tgatacagac ccctaaagaa gaaggtgcta atgtcctgac
1261 cacagaagcg ctcctacaac acctggactc ggcactccag gccagccgtg tccatgtata
1321 catgtacaac aggcagtgga aattggaaca tttgtgttac aaatcaggag agcttatcac
1381 agaaacaggt tacatggatc agataataga atatctttac ccttgtttga ttattacacc
1441 tttggactgc ttctgggaag gggcgaaatt acagtctggg acagcatacc tcctaggtaa
1501 acctcctttg cggtggacaa acttcgaccc tttggaattc ctggaagagt taaagaaaat
1561 aaactatcaa gtggacagct gggaggaaat gctgaataag gctgaggttg gtcatggtta
1621 catggaccgc ccctgcctca atccggccga tccagactgc cccgccacag cccccaacaa
1681 aaattcaacc aaacctcttg atatggccct tgttttgaat ggtggatgtc atggcttatc
1741 cagaaagtat atgcactggc aggaggagtt gattgtgggt ggcacagtca agaacagcac
1801 tggaaaactc gtcagcgccc atgccctgca gaccatgttc cagttaatga ctcccaagca
1861 aatgtacgag cacttcaagg ggtacgagta tgtctcacac atcaactgga acgaggacaa
1921 agcggcagcc atcctggagg cctggcagag gacatatgtg gaggtggttc atcagagtgt
1981 cgcacagaac tccactcaaa aggtgctttc cttcaccacc acgaccctgg acgacatcct
2041 gaaatccttc tctgacgtca gtgtcatccg cgtggccagc ggctacttac tcatgctcgc
2101 ctatgcctgt ctaaccatgc tgcgctggga ctgctccaag tcccagggtg ccgtggggct
2161 ggctggcgtc ctgctggttg cactgtcagt ggctgcagga ctgggcctgt gctcattgat
2221 cggaatttcc tttaacgctg caacaactca ggttttgcca tttctcgctc ttggtgttgg
2281 tgtggatgat gtttttcttc tggcccacgc cttcagtgaa acaggacaga ataaaagaat
2341 cccttttgag gacaggaccg gggagtgcct gaagcgcaca ggagccagcg tggccctcac
2401 gtccatcagc aatgtcacag ccttcttcat ggccgcgtta atcccaattc ccgctctgcg
2461 ggcgttctcc ctccaggcag cggtagtagt ggtgttcaat tttgccatgg ttctactcat
2521 ttttcctgca attctcagca tggatttata tcgacgcgag gacaggagac tggatatttt
2581 ctgctgtttt acaagcccct gcgtcagcag agtgattcag gttgaacctc aggcctacac
2641 cgacacacac gacaataccc gctacagccc cccacctccc tacagcagcc acagctttgc
2701 ccatgaaacg cagattacca tgcagtccac tgtccagctc cgcacggagt acgaccccca
2761 cacgcacgtg tactacacca ccgctgagcc gcgctccgag atctctgtgc agcccgtcac
2821 cgtgacacag gacaccctca gctgccagag cccagagagc accagctcca caagggacct
2881 gctctcccag ttctccgact ccagcctcca ctgcctcgag cccccctgta cgaagtggac
2941 actctcatct tttgctgaga agcactatgc tcctttcctc ttgaaaccaa aagccaaggt
3001 aagtatcaag gttacaagac aggtttaagg agaccaataa gctattattc gtataggata
3061 ctttatacga agttattaag ttatggagac cggtgtggca cgtgcggacc gagcggccgc
3121 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg
3181 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc
3241 gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg
3301 gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag
3361 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc
3421 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
3481 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
3541 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
3601 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
3661 actcaactct atctcgggct attcttttga tttataaggg attttgccga tttcggtcta
3721 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
3781 gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
3841 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
3901 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
3961 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
4021 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
4081 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
4141 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
4201 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
4261 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
4321 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
4381 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
4441 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
4501 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
4561 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
4621 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
4681 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
4741 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
4801 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
4861 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
4921 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
4981 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
5041 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
5101 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
5161 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
5221 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
5281 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
5341 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
5401 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
5461 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
5521 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
5581 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
5641 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
5701 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
5761 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
5821 acggttcctg gccttttgct ggccttttgc tcacatgt
//
pAAVLINK-EF1a-HA-5'-PTCH1载体图谱质粒图谱pdf版和pAAVLINK-EF1a-HA-5'-PTCH1载体序列质粒序列等相关资料下载
pAAVLINK-EF1a-HA-5'-PTCH1质粒载体应用举例
- 上一篇：pAAVLINKv2-SCP1-Cre-UDeg3a-3'-MYLK-FLAG
- 下一篇：没有了
