| 质粒名称: | pAAVLINK-EF1a-5'-COL1A1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001647 |
| 产品用途: | 5' AAVLINK plasmid for COL1A1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | COL1A1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-5'-COL1A1
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-5'-COL1A1载体质粒基本信息
pAAVLINK-EF1a-5'-COL1A1质粒图谱载体图谱和pAAVLINK-EF1a-5'-COL1A1载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-5'-COL1A1质粒载体简介
pAAVLINK-EF1a-5'-COL1A1质粒序列载体序列
LOCUS pAAVLINK-EF1a-5'-COL1A1 5826 bp DNA circular SYN 18-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5826)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 22, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5826
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 713..2966
/codon_start=1
/label=COL1A1
/translation="MFSFVDLRLLLLLAATALLTHGQEEGQVEGQDEDIPPITCVQNGL
RYHDRDVWKPEPCRICVCDNGKVLCDDVICDETKNCPGAEVPEGECCPVCPDGSESPTD
QETTGVEGPKGDTGPRGPRGPAGPPGRDGIPGQPGLPGPPGPPGPPGPPGLGGNFAPQL
SYGYDEKSTGGISVPGPMGPSGPRGLPGPPGAPGPQGFQGPPGEPGEPGASGPMGPRGP
PGPPGKNGDDGEAGKPGRPGERGPPGPQGARGLPGTAGLPGMKGHRGFSGLDGAKGDAG
PAGPKGEPGSPGENGAPGQMGPRGLPGERGRPGAPGPAGARGNDGATGAAGPPGPTGPA
GPPGFPGAVGAKGEAGPQGPRGSEGPQGVRGEPGPPGPAGAAGPAGNPGADGQPGAKGA
NGAPGIAGAPGFPGARGPSGPQGPGGPPGPKGNSGEPGAPGSKGDTGAKGEPGPVGVQG
PPGPAGEEGKRGARGEPGPTGLPGPPGERGGPGSRGFPGADGVAGPKGPAGERGSPGPA
GPKGSPGEAGRPGEAGLPGAKGLTGSPGSPGPDGKTGPPGPAGQDGRPGPPGPPGARGQ
AGVMGFPGPKGAAGEPGKAGERGVPGPPGAVGPAGKDGEAGAQGPPGPAGPAGERGEQG
PAGSPGFQGLPGPAGPPGEAGKPGEQGVPGDLGAPGPSGARGERGFPGERGVQGPPGPA
GPRGANGAPGNDGAKGDAGAPGAPGSQGAPGLQGMPGERGAAGLPGPKGDRGDAGPK"
misc_feature 763..768
/label=SDS
misc_feature 1448..1453
/label=SDS
misc_feature 1736..1741
/label=SDS
misc_feature 1988..1993
/label=SDS
intron 2967..3007
/label=Splicing Donor
misc_feature 3011..3044
/label=lox2272-JT1571
repeat_region 3089..3229
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3089..3218
/label=AAV2 ITR
rep_origin 3304..3759
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4041..4145
/gene="bla"
/label=AmpR promoter
CDS 4146..5006
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5177..5765
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgttcag
721 ctttgtggac ctccggctcc tgctcctctt agcggccacc gccctcctga cgcacggcca
781 agaggaaggc caagtcgagg gccaagacga agacatccca ccaatcacct gcgtacagaa
841 cggcctcagg taccatgacc gagacgtgtg gaaacccgag ccctgccgga tctgcgtctg
901 cgacaacggc aaggtgttgt gcgatgacgt gatctgtgac gagaccaaga actgccccgg
961 cgccgaagtc cccgagggcg agtgctgtcc cgtctgcccc gacggctcag agtcacccac
1021 cgaccaagaa accaccggcg tcgagggacc caagggagac actggccccc gaggcccaag
1081 gggacccgca ggcccccctg gccgagatgg catccctgga cagcctggac ttcccggacc
1141 ccccggaccc cccggacctc ccggaccccc tggcctcgga ggaaactttg ctccccagct
1201 gtcttatggc tatgatgaga aatcaaccgg aggaatttcc gtgcctggcc ccatgggtcc
1261 ctctggtcct cgtggtctcc ctggcccccc tggtgcacct ggtccccaag gcttccaagg
1321 tccccctggt gagcctggcg agcctggagc ttcaggtccc atgggtcccc gaggtccccc
1381 aggtccccct ggaaagaatg gagatgatgg ggaagctgga aaacctggtc gtcctggtga
1441 gcgtgggcct cctgggcctc agggtgctcg aggattgccc ggaacagctg gcctccctgg
1501 aatgaaggga cacagaggtt tcagtggttt ggatggtgcc aagggagatg ctggtcctgc
1561 tggtcctaag ggtgagcctg gcagccctgg tgaaaatgga gctcctggtc agatgggccc
1621 ccgtggcctg cctggtgaga gaggtcgccc tggagcccct ggccctgctg gtgctcgtgg
1681 aaatgatggt gctactggtg ctgccgggcc ccctggtccc accggccccg ctggtcctcc
1741 tggcttccct ggtgctgttg gtgctaaggg tgaagctggt ccccaagggc cccgaggctc
1801 tgaaggtccc cagggtgtgc gtggtgagcc tggcccccct ggccctgctg gtgctgctgg
1861 ccctgctgga aaccctggtg ctgatggaca gcctggtgct aaaggtgcca atggtgctcc
1921 tggtattgct ggtgctcctg gcttccctgg tgcccgaggc ccctctggac cccagggccc
1981 cggcggccct cctggtccca agggtaacag cggtgaacct ggtgctcctg gcagcaaagg
2041 agacactggt gctaagggag agcctggccc tgttggtgtt caaggacccc ctggccctgc
2101 tggagaggaa ggaaagcgag gagctcgagg tgaacccgga cccactggcc tgcccggacc
2161 ccctggcgag cgtggtggac ctggtagccg tggtttccct ggcgcagatg gtgttgctgg
2221 tcccaagggt cccgctggtg aacgtggttc tcctggccct gctggcccca aaggatctcc
2281 tggtgaagct ggtcgtcccg gtgaagctgg tctgcctggt gccaagggtc tgactggaag
2341 ccctggcagc cctggtcctg atggcaaaac tggcccccct ggtcccgccg gtcaagatgg
2401 tcgccccgga cccccaggcc cacctggtgc ccgtggtcag gctggtgtga tgggattccc
2461 tggacctaaa ggtgctgctg gagagcccgg caaggctgga gagcgaggtg ttcccggacc
2521 ccctggcgct gtcggtcctg ctggcaaaga tggagaggct ggagctcagg gaccccctgg
2581 ccctgctggt cccgctggcg agagaggtga acaaggccct gctggctccc ccggattcca
2641 gggtctccct ggtcctgctg gtcctccagg tgaagcaggc aaacctggtg aacagggtgt
2701 tcctggagac cttggcgccc ctggcccctc tggagcaaga ggcgagagag gtttccctgg
2761 cgagcgtggt gtgcaaggtc cccctggtcc tgctggtccc cgaggggcca acggtgctcc
2821 cggcaacgat ggtgctaagg gtgatgctgg tgcccctgga gctcccggta gccagggcgc
2881 ccctggcctt cagggaatgc ctggtgaacg tggtgcagct ggtcttccag ggcctaaggg
2941 tgacagaggt gatgctggtc ccaaaggtaa gtatcaaggt tacaagacag gtttaaggag
3001 accaataagc tattattcgt ataggatact ttatacgaag ttattaagtt atggagaccg
3061 gtgtggcacg tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc
3121 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc
3181 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg
3241 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
3301 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
3361 ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct tcctttctcg
3421 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
3481 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
3541 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
3601 gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat tcttttgatt
3661 tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat
3721 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
3781 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
3841 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
3901 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
3961 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
4021 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
4081 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
4141 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
4201 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
4261 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
4321 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
4381 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
4441 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
4501 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
4561 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
4621 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
4681 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
4741 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
4801 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
4861 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
4921 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
4981 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
5041 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
5101 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
5161 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
5221 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
5281 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
5341 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
5401 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
5461 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
5521 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
5581 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
5641 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
5701 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
5761 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
5821 acatgt
//
pAAVLINK-EF1a-5'-COL1A1载体图谱质粒图谱pdf版和pAAVLINK-EF1a-5'-COL1A1载体序列质粒序列等相关资料下载
pAAVLINK-EF1a-5'-COL1A1质粒载体应用举例
- 上一篇：pAAVLINKv2-SCP1-Cre-UDeg3a-3'-IGF1R-FLAG
- 下一篇：没有了
