PCDH-CMV-mCherry-TRIM71

LOCUS       PCDH-CMV-mCherry-TRIM71          9756 bp DNA     circular SYN 11-SEP-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9756)
  AUTHORS   15129015045
  TITLE     Direct Submission
  JOURNAL   Exported Aug 11, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9756
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1076..1309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1798..1915
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     1904..1953
                     /label=Primer 4
     enhancer        1938..2241
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2242..2445
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2444..2491)
                     /label=Primer 1
     primer_bind     2466..2500
                     /label=Primer 2
     primer_bind     2466..2492
                     /label=Primer 5
     CDS             2496..3203
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2878..2888)
                     /label=TRIM65-F
     primer_bind     3025..3046
                     /label=mcherry-F
     misc_feature    3204..3232
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     3218..3245
                     /label=TRIM65-F
     primer_bind     3223..3244
                     /label=TRIM71-F
     misc_feature    3240..5843
                     /label=trim71
     primer_bind     complement(5847..5864)
                     /label=TRIM71-R
     misc_feature    5853..6440
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(6324..6335)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6513..6746
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6818..6939
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      6958..7093
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7131..7147)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7155..7171
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7179..7209)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7224..7245
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7533..8121)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8292..9152)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9153..9257)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9626..9639)
                     /label=Primer 5
     primer_bind     9731..9747
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagttgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     1981 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     2041 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     2101 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     2161 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     2221 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     2281 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     2341 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     2401 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
     2461 cagattctag accgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggaggata
     2521 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     2581 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     2641 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     2701 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     2761 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     2821 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     2881 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     2941 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     3001 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     3061 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     3121 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     3181 gcggcatgga cgagctgtac aagtccggac tcagatctcg agctcaagct tcggaattca
     3241 tggcctcctt ccctgagaca gatttccaga tctgcctgct gtgcaaggag atgtgcggca
     3301 gccccgcccc cctgtcttcc aactcctccg ccagcagctc ctccagccag acctccacaa
     3361 gcagcggcgg cggcggagga ggaccaggag ctgctgctag aagactgcac gtgctgccct
     3421 gtctgcacgc cttctgtagg ccttgcctgg aggcccacag gctgcctgcc gctggaggag
     3481 gagccgctgg agagcctctg aagctgagat gtcctgtgtg tgatcagaag gtggtgctgg
     3541 ccgaggccgc cggaatggat gccctgcctt ccagcgcctt cctgctgagc aacctgctgg
     3601 acgccgtggt ggccaccgcc gatgagcctc ctcctaagaa tggcagggcc ggcgcccctg
     3661 ccggagctgg aggacactct aaccacagac accacgccca ccacgcccat cccagagcct
     3721 ccgcttccgc ccctcctctg ccccaagccc ctcagcctcc tgccccaagc agaagcgccc
     3781 ccggaggacc tgccgcttcc ccttctgccc tgctgctgag aagacctcac ggctgtagct
     3841 cctgcgacga gggcaacgcc gccagcagca gatgcctgga ttgtcaggag cacctgtgcg
     3901 acaactgcgt gagggcccac cagagagtga gactgacaaa ggatcactac atcgagaggg
     3961 gcccccctgg ccccggagct gctgccgctg ctcaacagct gggcctgggc cctccttttc
     4021 ccggcccacc tttctccatc ctgagcgtgt tccccgagag actgggcttt tgccagcacc
     4081 acgacgatga ggtgctgcac ctgtactgcg atacatgtag cgtgcccatc tgcagagagt
     4141 gtacaatggg cagacacggc ggccactcct tcatctacct gcaggaggcc ctgcaggatt
     4201 ccagagccct gaccatccag ctgctggccg acgcccagca gggcagacag gctatccagc
     4261 tgagcatcga gcaggcccag acagtggccg agcaggtgga gatgaaggcc aaggtggtgc
     4321 agtccgaggt gaaggccgtg accgccagac acaagaaggc cctggaggag agagagtgcg
     4381 agctgctgtg gaaggtggag aagatcaggc aggtgaaggc caagagcctg tacctgcagg
     4441 tggagaagct gagacagaac ctgaacaagc tggagtccac catcagcgcc gtgcagcagg
     4501 tgctggagga gggcagagcc ctggacatcc tgctggccag agacagaatg ctggcccagg
     4561 tgcaggagct gaagaccgtg aggagcctgc tgcagcccca ggaggatgac agggtcatgt
     4621 ttacccctcc cgaccaggcc ctgtacctgg ccatcaagag cttcggcttt gtgtcctccg
     4681 gcgccttcgc ccccctgaca aaggccacag gcgacggcct gaagagagcc ctgcagggca
     4741 aggtggccag cttcacagtg atcggctacg atcacgacgg cgagcccagg ctgtccggcg
     4801 gagatctgat gtccgccgtg gtgctgggcc ccgacggaaa tctgtttggc gccgaggtga
     4861 gcgaccagca gaacggcacc tacgtggtga gctacaggcc tcagctggag ggcgagcacc
     4921 tggtgtccgt gacactgtgt aatcagcaca tcgagaatag ccccttcaag gtggtggtga
     4981 agtccggcag gtcctacgtg ggcatcggcc tgcccggcct gtcctttggc tccgagggcg
     5041 attccgatgg caagctgtgc agaccttggg gcgtgtccgt ggacaaggag ggctacatca
     5101 tcgtggccga cagaagcaac aacagaatcc aggtgttcaa gccttgcggc gcctttcacc
     5161 acaagtttgg caccctgggc agcaggcctg gccagtttga tagacccgcc ggcgtggcct
     5221 gtgacgcctc tagaagaatc gtggtggccg acaaggacaa tcacaggatt caaatcttta
     5281 ccttcgaggg ccagtttctg ctgaagttcg gcgagaaggg cacaaagaac ggccagttta
     5341 actacccttg ggatgtggcc gtgaatagcg agggcaagat cctggtgagc gataccagga
     5401 accacagaat ccagctgttt ggccctgatg gcgtgttcct gaacaagtac ggctttgagg
     5461 gcgccctgtg gaagcacttt gatagcccta ggggcgtggc ctttaaccac gagggccacc
     5521 tggtggtgac cgattttaac aaccacaggc tgctggtcat tcaccccgac tgccagagcg
     5581 ccaggttcct gggcagcgag ggcacaggca atggccagtt cctgaggcct cagggcgtgg
     5641 ccgtggatca ggagggcaga atcatcgtgg ctgactccag gaatcacagg gtgcagatgt
     5701 ttgagagcaa tggctccttt ctgtgtaagt ttggcgccca gggctccggc tttggccaga
     5761 tggataggcc tagcggcatc gccatcaccc ccgatggcat gatcgtggtg gtggactttg
     5821 gcaataacag gatcttggtg ttctgaggat ccaatcaacc tctggattac aaaatttgtg
     5881 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     5941 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     6001 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     6061 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     6121 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     6181 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     6241 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     6301 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     6361 tgctgccggc tctgcggcct cttccgcgtc tccgccttcg ccctcagacg agtcggatct
     6421 ccctttggcc gcctccccgc cggtaccttt aagaccaatg acttacaagg cagctgtaga
     6481 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaa
     6541 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     6601 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     6661 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     6721 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     6781 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     6841 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     6901 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     6961 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     7021 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     7081 ttttttggag gcctagactt ttgcagagac ggcccaaatt cgtaatcatg gtcatagctg
     7141 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     7201 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     7261 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     7321 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     7381 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     7441 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     7501 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     7561 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     7621 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     7681 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     7741 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     7801 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     7861 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     7921 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
     7981 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     8041 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     8101 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     8161 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     8221 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     8281 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     8341 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     8401 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     8461 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     8521 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     8581 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     8641 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     8701 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     8761 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     8821 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     8881 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     8941 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     9001 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     9061 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     9121 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     9181 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     9241 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     9301 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
     9361 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     9421 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     9481 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     9541 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca
     9601 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     9661 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     9721 tcacgacgtt gtaaaacgac ggccagtgcc aagctg
//