pAAVLINKv2-SCP1-Cre-UDeg3a-3'-UBN2-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-3'-UBN2-FLAG                6837 bp DNA     circular SYN 24-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6837)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 10, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3550
                     /codon_start=1
                     /gene="UBN2"
                     /product="ubinuclein-2"
                     /label=UBN2
                     /db_xref="GI:236459851"
                     /db_xref="CCDS:CCDS43655.2"
                     /db_xref="GeneID:254048"
                     /db_xref="HGNC:HGNC:21931"
                     /db_xref="MIM:613841"
                     /protein_id="NP_775840.3"
                     /translation="VIPAPKPKVKEVMVKTLPLHSFPTMLKECSPKKDQKTPTSLVASV
                     SGPPTSSSTAAIAAASSSSAPAQETICLDDSLDEDLSFHSPSLDLVSEALAVINNGNKG
                     PPVGSRISMPTTKPRPGLREEKLASIMSKLPLATPKKLDSTQTTHSSSLIAGHTGPVPK
                     KPQDLAHTGISSGLIAGSSIQNPKVSLEPLPARLLQQGLQRSSQIHTSSSSQTHVSSSS
                     QAQIAASSHALGTSEAQDASSLTQVTKVHQHSAVQQNYVSPLQATISKSQTNPVVKLSN
                     NPQLSCSSSLIKTSDKPLMYRLPLSTPSPGNGSQGSHPLVSRTVPSTTTSSNYLAKAMV
                     SQISTQGFKSPFSMAASPKLAASPKPATSPKPLPSPKPSASPKPSLSAKPSVSTKLISK
                     SNPTPKPTVSPSSSSPNALVAQGSHSSTNSPVHKQPSGMNISRQSPTLNLLPSSRTSGL
                     PPTKNLQAPSKLTNSSSTGTVGKNSLSGIAMNVPASRGSNLNSSGANRTSLSGGTGSGT
                     QGATKPLSTPHRPSTASGSSVVTASVQSTAGASLLANASPLTLMTSPLSVTNQNVTPFG
                     MLGGLVPVTMPFQFPLEIFGFGTDTAGVTTTSGSTSAAFHHSLTQNLLKGLQPGGAQHA
                     ATLSHSPLPAHLQQAFHDGGQSKGDTKLPRKSQ"
     misc_feature    1575..1580
                     /label=SDS
     misc_feature    2409..2414
                     /label=SDS
     misc_feature    3433..3438
                     /label=SDS
     CDS             3557..3622
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3626..4057
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3770..3775
                     /label=SDS
     repeat_region   4100..4240
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4100..4229
                     /label=AAV2 ITR
     repeat_region   4101..4241
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4315..4770
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5052..5156
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5157..6017
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6188..6776
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtga ttcctgcacc
     1561 taaacccaaa gtaaaggagg tgatggtaaa gacccttcct ctccattctt tccccactat
     1621 gcttaaggag tgtagtccaa aaaaggacca gaaaactcca acatccctgg tggcttcggt
     1681 tagcggtcct ccaacgagct ccagcacagc tgccattgct gcagctagct ctagctctgc
     1741 accagcccaa gaaaccatct gcctcgacga ctcactagat gaagaccttt ctttccattc
     1801 accttcactg gatcttgttt ctgaagcttt agcggttatc aacaatggga acaagggccc
     1861 tccagttggc tcaaggataa gcatgccaac cacaaagcct cgtccaggac tgagagaaga
     1921 aaaattagca agtatcatga gtaagctgcc actagctact cccaaaaaac tagattctac
     1981 tcagactaca cattcttcaa gtcttattgc tggtcacaca gggccagtac caaagaaacc
     2041 ccaggattta gctcatactg gcatctcttc aggccttatt gctggttctt ccattcagaa
     2101 ccctaaagtt tctttagaac ctttgccagc caggctactt caacaaggac ttcagaggtc
     2161 aagccagatt cacacttctt cctcttcaca gacccatgtc tcctcttctt cccaagccca
     2221 aattgctgcc tcttctcatg ctctgggaac atccgaggcc caagatgctt cttcgttaac
     2281 acaagtaaca aaggtgcacc agcattcagc tgtccagcag aactatgtgt ctccattaca
     2341 ggccaccatc agtaaatccc agaccaaccc cgtcgtgaag ttaagtaata atccccaact
     2401 ctcctgttcc tcctcactta ttaagacttc agataagcca cttatgtacc gccttccctt
     2461 atctaccccc tcacctggaa atggttctca agggtcccac cccctggttt ctaggacagt
     2521 acctagcacc actacctcca gtaactattt agccaaggct atggtgtcac agatctccac
     2581 gcagggtttc aaatctccct tctcgatggc tgcctcccca aaacttgccg catctcccaa
     2641 gcctgccaca tctcctaaac ccctgccctc gcctaagcct tctgcctcac ccaagccctc
     2701 tctgtcagct aagccttcag tatcaactaa acttatttct aaatccaacc caactcccaa
     2761 gcctactgta tccccaagta gttccagtcc aaatgcacta gttgcccagg gtagccactc
     2821 cagcactaac agcccagtcc ataaacagcc cagtggaatg aacatcagca gacagtctcc
     2881 caccttgaat ttattgccct ctagtcgcac ttcaggcctt ccacctacaa aaaatcttca
     2941 ggccccctca aagctaacaa actcatcatc cactggaact gttgggaaga acagcttgag
     3001 tggaattgca atgaatgtac ctgccagcag aggtagcaac cttaactcaa gcggagctaa
     3061 taggactagt ctgtctgggg gaacaggaag tggaacacag ggtgctacca aaccattgtc
     3121 tactccacat agaccatcca ctgcctcagg gtcttcagtg gtaacagcca gtgtgcagtc
     3181 cacagcagga gcatcattat tggctaatgc ctcacctctg actctcatga catcaccttt
     3241 gtctgtaaca aatcaaaatg tgactccttt tgggatgctg ggtggccttg ttccagtgac
     3301 catgcccttc cagtttccct tggagatatt tggctttgga acggacacag ctggagtgac
     3361 aaccacctcg ggatctacct cagccgcttt ccaccatagc ctaactcaga atttactaaa
     3421 gggtttacag ccaggaggag ctcagcatgc agcaacgctt tcccactcac ctctgcctgc
     3481 acacttacag caagcatttc acgatggagg ccaaagtaaa ggggacacta aattaccacg
     3541 gaaatctcag tgtacagact acaaagacca tgacggtgat tataaagatc atgacatcga
     3601 ctacaaggat gacgatgaca agtaactcga gataatcaac ctctggatta caaaatttgt
     3661 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     3721 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     3781 aaatcctggt tagttcttgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     3841 acaggggctc ggctgttggg cactgacaat tccgtggtgt ttatttgtga aatttgtgat
     3901 gctattgctt tatttgtaac catctagctt tatttgtgaa atttgtgatg ctattgcttt
     3961 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     4021 gtttcaggtt cagggggaga tgtgggaggt tttttaaaga tctggagacc ggtgtggcac
     4081 gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc
     4141 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg
     4201 gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt
     4261 ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc
     4321 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     4381 ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     4441 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     4501 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc
     4561 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     4621 tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga
     4681 ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     4741 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg
     4801 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     4861 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     4921 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     4981 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     5041 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     5101 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     5161 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     5221 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     5281 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     5341 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     5401 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     5461 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     5521 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     5581 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     5641 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     5701 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     5761 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     5821 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     5881 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     5941 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     6001 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     6061 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     6121 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     6181 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     6241 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     6301 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     6361 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     6421 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     6481 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     6541 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     6601 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6661 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6721 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     6781 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//