pAAVLINKv2-SCP1-Cre-UDeg3a-ATR-C-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-ATR-C-FLAG               7148 bp DNA     circular SYN 26-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7148)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 9, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7148
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3861
                     /codon_start=1
                     /label=ATR
                     /translation="VTVLKKIL*TG*LD*K*PRIPTEPRSLSWLSGGLY*ASTKDQITM
                     KWLENAGCRVPG*LERLVTTRQPTMLSLMQGNHDSLNCTWKGQSGSGPRVMFTRH*LFF
                     KKVLNYVFLKMKPHLRVRTC*SMVELCY*WADLWKKQLTLKAMQL*KNIRM*PRACQNG
                     RMGIFTLPSTMTN*CPWSQTTKWKSKVISSGI*FFILADLYNMEISSYISQCHEC*LYG
                     LIMVQRHMNGKKLAAPIVYK*GMIWVK*TRLSQSIQTI*LHINF*LLFHN*SLEFVILT
                     MKFLLS*WK**PKYF*PILNKQCG**QLCQSHLIPCV*TDARKSSIKLFI*KNP*RSLL
                     EMQLA*QISF*NCAINRLMEVVPH*A*ALILKCLKSW*KKQHLVKSSFLYNQS*YLHFH
                     QFWVPMLTMLAMNHFLDIGPILQGLMIWWKFLLLFRNQRRFL*KAQMESSTS*CVSQKM
                     T*ERIVD*WNSIP*LISA*EKMQSLVEENFIFEHMQLFH*MMNVGLLNG*TTLLV*DLF
                     *PNYIKKRECI*QEKNFASVCYQSQQLYLKNSKYSENFSCPGILLFFMSGF*EHSLILH
                     HGTVVDQLTAVPLQ*CQWLVIFWGLETVMVKIFSLIL*LVNAYM*ISIVFSIREKPLKF
                     QKLCHFA*LIIWLMEWVLWEQRVFFEEHVKLQ*G*CVISESL**VS*RLFYMILLWNGV
                     NQ*KGIPKRH*MKLEKLSMKRPRPMFLTLSSDYKV*SRLEIE*QDCRYLLKDMCITLYR
                     KLLMKTYYARCILVGLHI"
     misc_feature    3226..3231
                     /label=SDS
     CDS             3868..3933
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3937..4368
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    4081..4086
                     /label=SDS
     repeat_region   4411..4551
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4411..4540
                     /label=AAV2 ITR
     rep_origin      4626..5081
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5363..5467
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5468..6328
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6499..7087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtga cagttctcaa
     1561 gaagattctc taaactgggt agctcgacta gaaatgaccc agaattccta cagagccaag
     1621 gagcctatcc tggctctccg gagggcttta ctaagcctca acaaaagacc agattacaat
     1681 gaaatggttg gagaatgctg gctgcagagt gccagggtag ctagaaaggc tggtcaccac
     1741 cagacagcct acaatgctct ccttaatgca ggggaatcac gactcgctga actgtacgtg
     1801 gaaagggcaa agtggctctg gtccaagggt gatgttcacc aggcactaat tgttcttcaa
     1861 aaaggtgttg aattatgttt tcctgaaaat gaaaccccac ctgagggtaa gaacatgtta
     1921 atccatggtc gagctatgct actagtgggc cgatttatgg aagaaacagc taactttgaa
     1981 agcaatgcaa ttatgaaaaa atataaggat gtgaccgcgt gcctgccaga atgggaggat
     2041 gggcattttt accttgccaa gtactatgac aaattgatgc ccatggtcac agacaacaaa
     2101 atggaaaagc aaggtgatct catccggtat atagttcttc attttggcag atctctacaa
     2161 tatggaaatc agttcatata tcagtcaatg ccacgaatgt taactctatg gcttgattat
     2221 ggtacaaagg catatgaatg ggaaaaagct ggccgctccg atcgtgtaca aatgaggaat
     2281 gatttgggta aaataaacaa ggttatcaca gagcatacaa actatttagc tccatatcaa
     2341 tttttgactg ctttttcaca attgatctct cgaatttgtc attctcacga tgaagttttt
     2401 gttgtcttga tggaaataat agccaaagta tttctagcct atcctcaaca agcaatgtgg
     2461 atgatgacag ctgtgtcaaa gtcatcttat cccatgcgtg tgaacagatg caaggaaatc
     2521 ctcaataaag ctattcatat gaaaaaatcc ttagagaagt ttgttggaga tgcaactcgc
     2581 ctaacagata agcttctaga attgtgcaat aaaccggttg atggaagtag ttccacatta
     2641 agcatgagca ctcattttaa aatgcttaaa aagctggtag aagaagcaac atttagtgaa
     2701 atcctcattc ctctacaatc agtcatgata cctacacttc catcaattct gggtacccat
     2761 gctaaccatg ctagccatga accatttcct ggacattggg cctatattgc agggtttgat
     2821 gatatggtgg aaattcttgc ttctcttcag aaaccaaaga agatttcttt aaaaggctca
     2881 gatggaaagt tctacatcat gatgtgtaag ccaaaagatg acctgagaaa ggattgtaga
     2941 ctaatggaat tcaattcctt gattaataag tgcttaagaa aagatgcaga gtctcgtaga
     3001 agagaacttc atattcgaac atatgcagtt attccactaa atgatgaatg tgggattatt
     3061 gaatgggtga acaacactgc tggtttgaga cctattctga ccaaactata taaagaaaag
     3121 ggagtgtata tgacaggaaa agaacttcgc cagtgtatgc taccaaagtc agcagcttta
     3181 tctgaaaaac tcaaagtatt ccgagaattt ctcctgccca ggcatcctcc tatttttcat
     3241 gagtggtttc tgagaacatt ccctgatcct acatcatggt acagtagtag atcagcttac
     3301 tgccgttcca ctgcagtaat gtcaatggtt ggttatattc tggggcttgg agaccgtcat
     3361 ggtgaaaata ttctctttga ttctttgact ggtgaatgcg tacatgtaga tttcaattgt
     3421 cttttcaata agggagaaac ctttgaagtt ccagaaattg tgccatttcg cctgactcat
     3481 aatatggtta atggaatggg tcctatggga acagagggtc tttttcgaag agcatgtgaa
     3541 gttacaatga ggctgatgcg tgatcagcga gagcctttaa tgagtgtctt aaagactttt
     3601 ctacatgatc ctcttgtgga atggagtaaa ccagtgaaag ggcattccaa agcgccactg
     3661 aatgaaactg gagaagttgt caatgaaaag gccaagaccc atgttcttga cattgagcag
     3721 cgactacaag gtgtaatcaa gactcgaaat agagtgacag gactgccgtt atctattgaa
     3781 ggacatgtgc attaccttat acaggaagct actgatgaaa acttactatg ccagatgtat
     3841 cttggttgga ctccatatat gtgtacagac tacaaagacc atgacggtga ttataaagat
     3901 catgacatcg actacaagga tgacgatgac aagtaactcg agataatcaa cctctggatt
     3961 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     4021 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     4081 cctccttgta taaatcctgg ttagttcttg ccacggcgga actcatcgcc gcctgccttg
     4141 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg tttatttgtg
     4201 aaatttgtga tgctattgct ttatttgtaa ccatctagct ttatttgtga aatttgtgat
     4261 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     4321 attcatttta tgtttcaggt tcagggggag atgtgggagg ttttttaaag atctggagac
     4381 cggtgtggca cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc
     4441 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg
     4501 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga
     4561 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc
     4621 atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     4681 gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc cttcctttct
     4741 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     4801 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag
     4861 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     4921 tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct attcttttga
     4981 tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga tttaacaaaa
     5041 atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac
     5101 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     5161 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     5221 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     5281 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     5341 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     5401 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     5461 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     5521 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     5581 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     5641 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     5701 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     5761 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     5821 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     5881 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     5941 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     6001 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     6061 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     6121 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     6181 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     6241 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     6301 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     6361 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     6421 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     6481 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     6541 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     6601 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     6661 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     6721 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     6781 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     6841 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     6901 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     6961 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     7021 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     7081 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     7141 tcacatgt
//