pGEX-2T-hsG3BP1

LOCUS       pGEX-2T-hsG3BP1                6351 bp DNA     circular SYN 24-NOV-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6351)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported Apr 17, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6351
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        183..211
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    219..235
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             258..911
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             918..938
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             942..2342
                     /codon_start=1
                     /label=hsG3BP1 no codon optimzed
                     /translation="MVMEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYVHGGL
                     DSNGKPADAVYGQKEIHRKVMSQNFTNCHTKIRHVDAHATLNDGVVVQVMGLLSNNNQA
                     LRRFMQTFVLAPEGSVANKFYVHNDIFRYQDEVFGGFVTEPQEESEEEVEEPEERQQTP
                     EVVPDDSGTFYDQAVVSNDMEEHLEEPVAEPEPDPEPEPEQEPVSEIQEEKPEPVLEET
                     APEDAQKSSSPAPADIAQTVQEDLRTFSWASVTSKNLPPSGAVPVTGIPPHVVKVPASQ
                     PRPESKPESQIPPQRPQRDQRVREQRINIPPQRGPRPIREAGEQGDIEPRRMVRHPDSH
                     QLFIGNLPHEVDKSELKDFFQSYGNVVELRINSGGKLPNFGFVVFDDSEPVQKVLSNRP
                     IMFRGEVRLNVEEKKTRAAREGDRRDNRLRGPGGPRGGLGGGMRGPPRGGMVQKPGFGV
                     GRGLAPRQ"
     promoter        2654..2758
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2759..3619
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3790..4378
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        4622..4699
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             4700..5782
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     protein_bind    5795..5816
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5831..5861
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5869..5885
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5893..5909
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5905..6351
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGI"
     primer_bind     complement(5925..5941)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
       61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
      121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
      181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
      241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
      301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
      361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
      421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
      481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
      541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
      601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
      661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
      721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
      781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
      841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
      901 atcctccaaa atcggatgaa aacctgtatt ttcagggatc catggtgatg gagaagccta
      961 gtcccctgct ggtcgggcgg gaatttgtga gacagtatta cacactgctg aaccaggccc
     1021 cagacatgct gcatagattt tatggaaaga actcttctta tgtccatggg ggattggatt
     1081 caaatggaaa gccagcagat gcagtctacg gacagaaaga aatccacagg aaagtgatgt
     1141 cacaaaactt caccaactgc cacaccaaga ttcgccatgt tgatgctcat gccacgctaa
     1201 atgatggtgt ggtagtccag gtgatggggc ttctctctaa caacaaccag gctttgagga
     1261 gattcatgca aacgtttgtc cttgctcctg aggggtctgt tgcaaataaa ttctatgttc
     1321 acaatgatat cttcagatac caagatgagg tctttggtgg gtttgtcact gagcctcagg
     1381 aggagtctga agaagaagta gaggaacctg aagaaagaca gcaaacacct gaggtggtac
     1441 ctgatgattc tggaactttc tatgatcagg cagttgtcag taatgacatg gaagaacatt
     1501 tagaggagcc tgttgctgaa ccagagcctg atcctgaacc agaaccagaa caagaacctg
     1561 tatctgaaat ccaagaggaa aagcctgagc cagtattaga agaaactgcc cctgaggatg
     1621 ctcagaagag ttcttctcca gcacctgcag acatagctca gacagtacag gaagacttga
     1681 ggacattttc ttgggcatct gtgaccagta agaatcttcc acccagtgga gctgttccag
     1741 ttactgggat accacctcat gttgttaaag taccagcttc acagccccgt ccagagtcta
     1801 agcctgaatc tcagattcca ccacaaagac ctcagcggga tcaaagagtg cgagaacaac
     1861 gaataaatat tcctccccaa aggggaccca gaccaatccg tgaggctggt gagcaaggtg
     1921 acattgaacc ccgaagaatg gtgagacacc ctgacagtca ccaactcttc attggcaacc
     1981 tgcctcatga agtggacaaa tcagagctta aagatttctt tcaaagttat ggaaacgtgg
     2041 tggagttgcg cattaacagt ggtgggaaat tacccaattt tggttttgtt gtgtttgatg
     2101 attctgagcc tgttcagaaa gtccttagca acaggcccat catgttcaga ggtgaggtcc
     2161 gtctgaatgt cgaagagaag aagactcgag ctgccaggga aggcgaccga cgagataatc
     2221 gccttcgggg acctggaggc cctcgaggtg ggctgggtgg tggaatgaga ggccctcccc
     2281 gtggaggcat ggtgcagaaa ccaggatttg gagtgggaag ggggcttgcg ccacggcagt
     2341 gagaattcat cgtgactgac tgacgatctg cctcgcgcgt ttcggtgatg acggtgaaaa
     2401 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     2461 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac
     2521 ccagtcacgt agcgatagcg gagtgtataa ttcttgaaga cgaaagggcc tcgtgatacg
     2581 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     2641 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     2701 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     2761 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     2821 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     2881 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     2941 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     3001 tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     3061 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     3121 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     3181 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     3241 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     3301 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     3361 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     3421 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     3481 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     3541 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     3601 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     3661 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     3721 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3781 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3841 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3901 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     3961 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     4021 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     4081 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     4141 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     4201 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4261 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4321 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     4381 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     4441 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     4501 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     4561 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcataaattc
     4621 cgacaccatc gaatggtgca aaacctttcg cggtatggca tgatagcgcc cggaagagag
     4681 tcaattcagg gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg
     4741 tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac
     4801 gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca
     4861 acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca
     4921 cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt
     4981 ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct
     5041 tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat
     5101 tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac
     5161 acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct
     5221 ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc
     5281 gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc
     5341 ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa
     5401 tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat
     5461 gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga
     5521 cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg
     5581 cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa
     5641 gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac
     5701 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     5761 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     5821 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     5881 aacaatttca cacaggaaac agctatgacc atgattacgg attcactggc cgtcgtttta
     5941 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     6001 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     6061 cgcagcctga atggcgaatg gcgctttgcc tggtttccgg caccagaagc ggtgccggaa
     6121 agctggctgg agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc aaactggcag
     6181 atgcacggtt acgatgcgcc catctacacc aacgtaacct atcccattac ggtcaatccg
     6241 ccgtttgttc ccacggagaa tccgacgggt tgttactcgc tcacatttaa tgttgatgaa
     6301 agctggctac aggaaggcca gacgcgaatt atttttgatg gcgttggaat t
//