pAAVLINKv2-SCP1-Cre-UDeg3a-3'-OTOF-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-3'-OTOF-FLAG          7329 bp DNA     circular SYN 25-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7329)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 15, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..4042
                     /codon_start=1
                     /gene="OTOF"
                     /gene_synonym="AUNB1; DFNB6; DFNB9; FER1L2; NSRD9"
                     /product="otoferlin isoform a"
                     /label=OTOF
                     /note="isoform a is encoded by transcript variant 
                     1;fer-1-like protein 2; fer-1-like family member 2"
                     /db_xref="GI:34740331"
                     /db_xref="CCDS:CCDS1725.1"
                     /db_xref="GeneID:9381"
                     /db_xref="HGNC:HGNC:8515"
                     /db_xref="MIM:603681"
                     /protein_id="NP_919224.1"
                     /translation="GVQSSLIHNYKKNPNFNTLVKWFEVDLPENELLHPPLNIRVVDCR
                     AFGRYTLVGSHAVSSLRRFIYRPPDRSAPSWNTTVRLLRRCRVLCNGGSSSHSTGEVVV
                     TMEPEVPIKKLETMVKLDATSEAVVKVDVAEEEKEKKKKKKGTAEEPEEEEPDESMLDW
                     WSKYFASIDTMKEQLRQQEPSGIDLEEKEEVDNTEGLKGSMKGKEKARAAKEEKKKKTQ
                     SSGSGQGSEAPEKKKPKIDELKVYPKELESEFDNFEDWLHTFNLLRGKTGDDEDGSTEE
                     ERIVGRFKGSLCVYKVPLPEDVSREAGYDSTYGMFQGIPSNDPINVLVRVYVVRATDLH
                     PADINGKADPYIAIRLGKTDIRDKENYISKQLNPVFGKSFDIEASFPMESMLTVAVYDW
                     DLVGTDDLIGETKIDLENRFYSKHRATCGIAQTYSTHGYNIWRDPMKPSQILTRLCKDG
                     KVDGPHFGPPGRVKVANRVFTGPSEIEDENGQRKPTDEHVALLALRHWEDIPRAGCRLV
                     PEHVETRPLLNPDKPGIEQGRLELWVDMFPMDMPAPGTPLDISPRKPKKYELRVIIWNT
                     DEVVLEDDDFFTGEKSSDIFVRGWLKGQQEDKQDTDVHYHSLTGEGNFNWRYLFPFDYL
                     AAEEKIVISKKESMFSWDETEYKIPARLTLQIWDADHFSADDFLGAIELDLNRFPRGAK
                     TAKQCTMEMATGEVDVPLVSIFKQKRVKGWWPLLARNENDEFELTGKVEAELHLLTAEE
                     AEKNPVGLARNEPDPLEKPNRPDTSFIWFLNPLKSARYFLWHTYRWLLLKLLLLLLLLL
                     LLALFLYSVPGYLVKKILGA"
     misc_feature    1827..1832
                     /label=SDS
     misc_feature    1950..1955
                     /label=SDS
     misc_feature    2001..2006
                     /label=SDS
     misc_feature    2004..2009
                     /label=SDS
     misc_feature    2118..2123
                     /label=SDS
     misc_feature    2385..2390
                     /label=SDS
     misc_feature    2697..2702
                     /label=SDS
     misc_feature    3360..3365
                     /label=SDS
     misc_feature    3724..3729
                     /label=SDS
     misc_feature    3801..3806
                     /label=SDS
     misc_feature    3979..3984
                     /label=SDS
     misc_feature    3982..3987
                     /label=SDS
     CDS             4049..4114
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      4118..4549
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    4262..4267
                     /label=SDS
     repeat_region   4592..4732
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4592..4721
                     /label=AAV2 ITR
     repeat_region   4593..4733
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4807..5262
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5544..5648
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5649..6509
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6680..7268
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggggg tgcagtcgtc
     1561 cctgatccac aattataaga agaaccccaa cttcaacacc ctcgtcaagt ggtttgaagt
     1621 ggacctccca gagaacgagc tgctgcaccc gcccttgaac atccgtgtgg tggactgccg
     1681 ggccttcggt cgctacacac tggtgggctc ccatgccgtc agctccctgc gacgcttcat
     1741 ctaccggccc ccagaccgct cggcccccag ctggaacacc acggtcaggc ttctccggcg
     1801 ctgccgtgtg ctgtgcaatg ggggctcctc ctctcactcc acaggggagg ttgtggtgac
     1861 tatggagcca gaggtaccca tcaagaaact ggagaccatg gtgaagctgg acgcgacttc
     1921 tgaagctgtt gtcaaggtgg atgtggctga ggaggagaag gagaagaaga agaagaagaa
     1981 gggcactgcg gaggagccag aggaggagga gccagacgag agcatgctgg actggtggtc
     2041 caagtacttt gcctccattg acaccatgaa ggagcaactt cgacaacaag agccctctgg
     2101 aattgacttg gaggagaagg aggaagtgga caataccgag ggcctgaagg ggtcaatgaa
     2161 gggcaaggag aaggcaaggg ctgccaaaga ggagaagaag aagaaaactc agagctctgg
     2221 ctctggccag gggtccgagg cccccgagaa gaagaaaccc aagattgatg agcttaaggt
     2281 ataccccaaa gagctggagt ccgagtttga taactttgag gactggctgc acactttcaa
     2341 cttgcttcgg ggcaagaccg gggatgatga ggatggctcc accgaggagg agcgcattgt
     2401 gggacgcttc aagggctccc tctgcgtgta caaagtgcca ctcccagagg acgtgtcccg
     2461 ggaagccggc tacgactcca cctacggcat gttccagggc atcccgagca atgaccccat
     2521 caatgtgctg gtccgagtct atgtggtccg ggccacggac ctgcaccctg ctgacatcaa
     2581 cggcaaagct gacccctaca tcgccatccg gctaggcaag actgacatcc gcgacaagga
     2641 gaactacatc tccaagcagc tcaaccctgt ctttgggaag tcctttgaca tcgaggcctc
     2701 cttccccatg gaatccatgc tgacggtggc tgtgtatgac tgggacctgg tgggcactga
     2761 tgacctcatt ggggaaacca agatcgacct ggagaaccgc ttctacagca agcaccgcgc
     2821 cacctgcggc atcgcccaga cctactccac acatggctac aatatctggc gggaccccat
     2881 gaagcccagc cagatcctga cccgcctctg caaagacggc aaagtggacg gcccccactt
     2941 tgggccccct gggagagtga aggtggccaa ccgcgtcttc actgggccct ctgagattga
     3001 ggacgagaac ggtcagagga agcccacaga cgagcatgtg gcgctgttgg ccctgaggca
     3061 ctgggaggac atcccccgcg caggctgccg cctggtgcca gagcatgtgg agacgaggcc
     3121 gctgctcaac cccgacaagc cgggcatcga gcagggccgc ctggagctgt gggtggacat
     3181 gttccccatg gacatgccag cccctgggac gcctctggac atctcacctc ggaagcccaa
     3241 gaagtacgag ctgcgggtca tcatctggaa cacagatgag gtggtcttgg aggacgacga
     3301 cttcttcaca ggggagaagt ccagtgacat cttcgtgagg gggtggctga agggccagca
     3361 ggaggacaag caggacacag acgtccacta ccactccctc actggcgagg gcaacttcaa
     3421 ctggcgctac ctgttcccct tcgactacct ggcggcggag gagaagatcg tcatctccaa
     3481 gaaggagtcc atgttctcct gggacgagac cgagtacaag atccccgcgc ggctcaccct
     3541 gcagatctgg gatgcggacc acttctccgc tgacgacttc ctgggggcca tcgagctgga
     3601 cctgaaccgg ttcccgcggg gcgcaaagac agccaagcag tgcaccatgg agatggccac
     3661 cggggaggtg gacgtgcccc tcgtgtccat cttcaagcaa aagcgcgtca aaggctggtg
     3721 gcccctcctg gcccgcaatg agaacgatga gtttgagctc acgggcaagg tggaggctga
     3781 gctgcattta ctgacagcag aggaggcaga gaagaaccca gtgggcctgg cccgcaatga
     3841 acctgacccc ctagagaaac ccaaccggcc cgacacgagc ttcatctggt tcctgaaccc
     3901 tctcaagtcg gctcgctact tcttgtggca cacgtatcgc tggctgctcc tcaaactgtt
     3961 gctgctcctg ctgctgctcc tcctcctcgc cctgttcctc tactctgtgc ctggctacct
     4021 ggtcaagaaa atcctcgggg cctgtacaga ctacaaagac catgacggtg attataaaga
     4081 tcatgacatc gactacaagg atgacgatga caagtaactc gagataatca acctctggat
     4141 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     4201 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     4261 tcctccttgt ataaatcctg gttagttctt gccacggcgg aactcatcgc cgcctgcctt
     4321 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gtttatttgt
     4381 gaaatttgtg atgctattgc tttatttgta accatctagc tttatttgtg aaatttgtga
     4441 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     4501 cattcatttt atgtttcagg ttcaggggga gatgtgggag gttttttaaa gatctggaga
     4561 ccggtgtggc acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact
     4621 ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg
     4681 ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg
     4741 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac
     4801 catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     4861 tgaccgctac acttgccagc gccttagcgc ccgctccttt cgctttcttc ccttcctttc
     4921 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     4981 gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta
     5041 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     5101 atagtggact cttgttccaa actggaacaa cactcaactc tatctcgggc tattcttttg
     5161 atttataagg gattttgccg atttcggtct attggttaaa aaatgagctg atttaacaaa
     5221 aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta
     5281 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     5341 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     5401 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     5461 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     5521 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     5581 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     5641 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     5701 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     5761 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     5821 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     5881 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     5941 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     6001 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     6061 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     6121 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     6181 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     6241 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     6301 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     6361 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     6421 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     6481 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     6541 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     6601 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     6661 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     6721 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     6781 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     6841 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     6901 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     6961 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7021 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7081 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7141 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7201 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7261 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     7321 ctcacatgt
//