| 质粒名称: | pAAV-mTH-Cre |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000858 |
| 产品用途: | express cre in TH+ neurons |
| 存储实验室: | Wenjie BIAN Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒，哺乳动物表达 |
| 启动子: | TH |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
|
插入基因信息 |
|
| 插入基因名称: | mTH-Cre |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Adolescent sleep shapes social novelty preference in mice.
Bian, W. J., Brewer, C. L., Kauer, J. A. & de Lecea, L.
Nat. Neurosci. 2022 257 25, 912–923 (2022)
|
买家导航
pAAV-mTH-Cre载体质粒基本信息
pAAV-mTH-Cre质粒图谱载体图谱和pAAV-mTH-Cre载体序列质粒序列多克隆位点信息
pAAV-mTH-Cre质粒载体简介
pAAV-mTH-Cre质粒序列载体序列
LOCUS pAAV-mTH-Cre 6131 bp DNA circular SYN 17-AUG-2023
DEFINITION An AAV genome that expresses GFP from the mouse TH promoter.
ACCESSION .
VERSION .
KEYWORDS pAAV-mTH-Cre
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6131)
AUTHORS Chan KY, Jang MJ, Yoo BB, Greenbaum A, Ravi N, Wu WL,
Sanchez-Guardado L, Lois C, Mazmanian SK, Deverman BE, Gradinaru V
TITLE Engineered AAVs for efficient noninvasive gene delivery to the
central and peripheral nervous systems.
JOURNAL Nat Neurosci. 2017 Aug;20(8):1172-1179. doi: 10.1038/nn.4593. Epub
2017 Jun 26.
PUBMED 28671695
REFERENCE 2 (bases 1 to 6131)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 1, 2026 from SnapGene 6.0.2
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn.4593";
journalName: "Nat Neurosci"; date: "2017-08"; volume: "20"; issue:
"8"; pages: "1172-1179"
FEATURES Location/Qualifiers
source 1..6131
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 16..145
/label=AAV2 IRT (alternative)
promoter 151..2722
/label=mouse TH/Mbp promoter
primer_bind 571..590
/label=rTH-F1
primer_bind 1265..1284
/label=rTH-F2
primer_bind complement(1339..1358)
/label=mTH-R
primer_bind 1914..1933
/label=mTH-F3
primer_bind 2639..2658
/label=mTH-F4
primer_bind 2723..2751
/label=3-F-NotI
regulatory 2726..2731
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 2732..3763
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
primer_bind complement(3744..3769)
/label=3-R-KpnI
polyA_signal 3785..3906
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3796..3815)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 3850..3869
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
repeat_region 4009..4138
/label=AAV2 ITR
primer_bind complement(4203..4221)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 4289..4393
/gene="bla"
/label=AmpR promoter
CDS 4394..5254
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4612..4631)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 5425..6013
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5914..5933
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc
61 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag agagggagtg
121 gccaactcca tcactagggg ttcctactag ttaaggggcc tcttggagtt gttctcatca
181 aatggggatg ggacacagct aaagtgccca gggcttctct gtgcccacag atgctttaga
241 tcttggcaca gtgtggtcta ccagctgtct ctctctgtgt atatatatgt atttcataga
301 cagtgtacag tggcctggtt tgtgctatca ggctggatat ggacagaggc aagagtttgt
361 ggcagcagtt atctcccaag agagtccaaa gacatcatgt tttcaagttt aggccaggtg
421 ctacttgaga gagctcagac acagacaaag gtctggagag cacatgtcct ccacccccac
481 ctagcttctg ttgcaagcac ctccagccga gacaagagaa cgaattaaaa agcaatattt
541 gtgtcagtgt aagacatttg ccgaaaggtt aaatccacat tcgtgttgct gcagagcagc
601 cccctatgca ggatttgtta gatacagctc cgtcctaccc tgtgccagct gagcaaacgc
661 caggctgggt ggggtggaac ccagcctggg tttgcctcac cctgcaatcc ccccagcacc
721 ctctaaagga ggaccctgtg gtgggcatgc agacctaggg actgggcata gataaccttt
781 gggtttgggc aacagccccc actcctcagg attgaaggct aaggtgcagc cagctctgcc
841 ttcatggtgg gaatgtctcc acgtgacccc tttctgggct gtggagaaca ctcagagaag
901 agtcctggga tgccaggcag gccagggatg tgctgggcat gttgagacag gagtgggcta
961 agccagcaga gttgctgacc caggaagagt tcagaaaggg gcatggaaca tggggagggg
1021 tccatagtga gagagagcag gcagtgcaga gtaaatagtc cctgagctgg gggttatggg
1081 atttgcagga gcttgctcag agaaggcaga ggagagatgc tgcgccaagc tgggtatcac
1141 agagcctcag actcctggaa caggaactgt gggggtcagg tcagcagggg aggttaggga
1201 gtgttccctt tgtactgact tagcatttat cctgcttcta ggggggaagg ggggccagtg
1261 ggggatgcac agcaaggcag tgatgtggca ggcagcctgc gggagctcct ggttcctggt
1321 gtgaaaaagc tgggaaggaa gagggctggg tctggtaagt acagcaggca gttggctcct
1381 gagagtccaa gccctgtcta gagggtggag tgagatttca gagggagagc taaacggggt
1441 gggggctggg gagtccaggc ttctggctcc tgctaatact cagtgtgctg ggtcctcaga
1501 acctcagggt ggccattttc agggtgagag ctctgtcctt tggcacttct gcagactcca
1561 gtatccagag gaataaagat ggtactcttc ctcagttccc ttagtgagag gacacctttc
1621 tctgaagggc ttgggcagtt gtcctgaacc attgcctgaa ggaaggactt gactccaggg
1681 acatagaatg ggctcagcat aagtcccctg tagtagagaa aggtcccctc tctggtctcc
1741 ttagagatcc tgtttccttg gctgaggaag ctagggtgga tctttgtgta agtgggtgtg
1801 gatgctcact ggaaatcaaa aggccccttg gtgttagacc ttggggtgcc atgggagagt
1861 tgatcactga gtgcgccctt acatgggggc cagctgagaa tggggctgcc tctagctcga
1921 gaccatgatg cagggagtga gtgggggagt tcaggatact cttaactaaa gcagaggtct
1981 gtccccccag ggaggggagg tcagaagacc ctagggagat gccaaaggct agggttggca
2041 ccatgttgca ggctgtgtct tcaaggagat gataatcaga ggaatcgaac ctgcaaaagt
2101 gggccagtct tagatacact atagaggaat aatcttctga aacattctgt gtctcatagg
2161 acctgcctga ggacccagcc ccagtgccag cacatacact ggggcagtga gtagatagta
2221 tactttgtta catgggctgg ggggacatgg cctgtgccct ggaggggact tgaagacatc
2281 caaaaagcta gtgagagggc tcctagattt atttgtctcc aagggctata tatagccttc
2341 ctaacatgaa cccttgggta atccagcatg ggcgctccca tatgccctgg tttgattaga
2401 gagctctaga tgtctcctgt cccagaacac cagccagccc ctgtcttcat gtcgtgtcta
2461 gggcggaggg tgattcagag gcaggtgcct gcgacagtgg atgcaattag atctaatggg
2521 acggaggcct ctctcgtccg tcgccctcgc tctgtgccca cccccgcctc cctcaggcac
2581 agcaggcgtg gagaggatgc gcaggaggta ggaggtgggg gacccagagg ggctttgacg
2641 tcagcctggc ctttaagagg ccgcctgcct ggcaagggct gtggagacag aactcgggac
2701 caccagcttg cactatgccc acgcggccgc catgtccaat ttactgaccg tacaccaaaa
2761 tttgcctgca ttaccggtcg atgcaacgag tgatgaggtt cgcaagaacc tgatggacat
2821 gttcagggat cgccaggcgt tttctgagca tacctggaaa atgcttctgt ccgtttgccg
2881 gtcgtgggcg gcatggtgca agttgaataa ccggaaatgg tttcccgcag aacctgaaga
2941 tgttcgcgat tatcttctat atcttcaggc gcgcggtctg gcagtaaaaa ctatccagca
3001 acatttgggc cagctaaaca tgcttcatcg tcggtccggg ctgccacgac caagtgacag
3061 caatgctgtt tcactggtta tgcggcggat ccgaaaagaa aacgttgatg ccggtgaacg
3121 tgcaaaacag gctctagcgt tcgaacgcac tgatttcgac caggttcgtt cactcatgga
3181 aaatagcgat cgctgccagg atatacgtaa tctggcattt ctggggattg cttataacac
3241 cctgttacgt atagccgaaa ttgccaggat cagggttaaa gatatctcac gtactgacgg
3301 tgggagaatg ttaatccata ttggcagaac gaaaacgctg gttagcaccg caggtgtaga
3361 gaaggcactt agcctggggg taactaaact ggtcgagcga tggatttccg tctctggtgt
3421 agctgatgat ccgaataact acctgttttg ccgggtcaga aaaaatggtg ttgccgcgcc
3481 atctgccacc agccagctat caactcgcgc cctggaaggg atttttgaag caactcatcg
3541 attgatttac ggcgctaagg atgactctgg tcagagatac ctggcctggt ctggacacag
3601 tgcccgtgtc ggagccgcgc gagatatggc ccgcgctgga gtttcaatac cggagatcat
3661 gcaagctggt ggctggacca atgtaaatat tgtcatgaac tatatccgta acctggatag
3721 tgaaacaggg gcaatggtgc gcctgctgga agatggcgat tagggtacct tcgagcagac
3781 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
3841 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
3901 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag
3961 gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcaagcttag gaacccctag
4021 tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa
4081 aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagag
4141 agggagtggc caagctagcg ggcgattaag gaaagggcta gatcattctt gaagacgaaa
4201 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
4261 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
4321 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
4381 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
4441 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
4501 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
4561 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
4621 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc
4681 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
4741 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
4801 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
4861 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
4921 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
4981 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
5041 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
5101 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
5161 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
5221 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
5281 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
5341 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
5401 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
5461 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
5521 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
5581 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
5641 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
5701 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
5761 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
5821 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
5881 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
5941 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
6001 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
6061 ttttgctcac atgtaataaa cacacacaca ccaacaaccg tggttggttg ttgtgttggt
6121 ttattctcga g
//
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