pHGZ-GKH

LOCUS       pHGZ-GKH            7163 bp DNA     circular SYN 22-JUL-2026
DEFINITION  2763_TSM20260703-0571-00691_20260704-0571-BAN17-1_C09.1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7163)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 30, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7163
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        1008..1083
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     terminator      1087..1106
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     rep_origin      complement(1444..2032)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2203..3063)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     terminator      3449..3468
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     misc_RNA        complement(3472..3547)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(3568..3836)
                     /label=SNR52 promoter
                     /note="promoter for the S. cerevisiae small nucleolar RNA 
                     gene SNR52"
     rep_origin      4152..5029
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     gene            complement(5532..6888)
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     terminator      5532..5729
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(5735..6544)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        complement(6545..6888)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     promoter        6895..7163
                     /label=SNR52 promoter
                     /note="promoter for the S. cerevisiae small nucleolar RNA 
                     gene SNR52"
ORIGIN
        1 tgagacccag gtcgaccgac agccttccaa atgttcttct caaacggaat cgtcgtatcc
       61 agcctactcg ctattgtcct caatgccgta ttaaatcata aaaagaaata agaaaaagag
      121 gtgcgagcct cttttttgtg tgacaaaata aaaacatcta cctattcata tacgctagtg
      181 tcatagtcct gaaaatcatc tgcatcaaga acaatttcac aactcttata cttttctctt
      241 acaagtcgtt cggcttcatc tggattttca gcctctatac ttactaaacg tgataaagtt
      301 tctgtaattt ctactgtatc gacctgcaga ctggctgtgt ataagggagc ctgacattta
      361 tattccccag aacatcaggt taatggcgtt ttgatgtcat tttcgcggtg gctgagatca
      421 gccacttctt ccccgataac ggagaccggc acactggcca tatcggtggt catcatgcgc
      481 cagctttcat ccccgatatg caccaccggg taaagttcac gggagacttt atctgacagc
      541 agacgtgcac tggccagggg gatcaccatc cgtcgcccgg gcgtgtcaat aatatcactc
      601 tgtacatcca caaacagacg ataacggctc tctcttttat aggtgtaaac cttaaactgc
      661 atttcaccag cccctgttct cgtcagcaaa agagccgttc atttcaataa accgggcgac
      721 ctcagccatc ccttcctgat tttccgcttt ccagcgttcg gcacgcagac gacgggcttc
      781 attctgcatg gttgtgctta ccagaccgga gatattgaca tcatatatgc cttgagcaac
      841 tgatagctgt cgctgtcaac tgtcactgta atacgctgct tcatagcata cctctttttg
      901 acatacttcg ggtatacata tcagtatata ttcttatacc gcaaaaatca gcgcgcaaat
      961 acgcatactg ttatctggct tttagtaagc cggatccctg ggtctcggtt ttagagctag
     1021 aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
     1081 tggtgctttt tttgtttttt atgtctagat cctaggatcc aagcttggta ccgagctcgt
     1141 cgacgaattc tgcagatatc catcacactg gcggccgctc gagcatgcat ctagagggcc
     1201 gcaggccctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     1261 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     1321 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     1381 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     1441 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     1501 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     1561 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     1621 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     1681 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     1741 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     1801 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     1861 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     1921 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     1981 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     2041 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     2101 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     2161 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     2221 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     2281 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     2341 gcgtgaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     2401 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     2461 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     2521 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     2581 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     2641 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     2701 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     2761 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     2821 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     2881 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     2941 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     3001 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     3061 catactcttc ctttttcaat gggtaataac tgatataatt aaattgaagc tctaatttgt
     3121 gagtttagta tacatgcatt tacttataat acagtttttt gaaaagctag cttatcgatg
     3181 ataagctgtc aaacatgaga attaattcca cggactatag actataccta gtatactccg
     3241 tctactgtac gatacacttc cgctcaggtc cttgtccttt aacgaggcct taccactctt
     3301 ttgttactct attgatccag ctcagcaaag gcagtgtgat ctaagattct atcttcgcga
     3361 tgtagtaaaa ctagctagac cgagaaagag actagaaatg caaaaggcac ttctacaatg
     3421 gctgccatca ttattatccg atgtgacgag acataaaaaa caaaaaaagc accaccgact
     3481 cggtgccact ttttcaagtt gataacggac tagccttatt ttaacttgct atttctagct
     3541 ctaaaacaga gctgcatcag gtcggaggat catttatctt tcactgcgga gaagtttcga
     3601 acgccgaaac atgcgcacca actttcactt ctacagcgtt tgaccaaaat cttttgaaca
     3661 gaacattgta gggtgtgaaa aaatgcgcac ctttaccgct agcccaagag ggcactacaa
     3721 aatctagagt tgtacttcaa acgtacatgt aatcaccttg tatatactcg aaagaaaaca
     3781 tcaagtttct gtataaatat gagtgaaagc ataatcatac attatctttt caaagactgc
     3841 agcttctcaa tgatattcga atacgctttg aggagataca gcctaatatc cgacaaactg
     3901 ttttacagat ttacgatcgt acttgttacc catcattgaa ttttgaacat ccgaacctgg
     3961 gagttttccc tgaaacagat agtatatttg aacctgtata ataatatata gtctagcgct
     4021 ttacggaaga caatgtatgt atttcggttc ctggagaaac tattgcatct attgcatagg
     4081 taatcttgca cgtcgcatcc ccggttcatt ttctgcgttt ccatcttgca cttcaatagc
     4141 atatctttgt taacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag
     4201 cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga
     4261 aagcgctatt ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc
     4321 gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa
     4381 cgcgagagcg ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc
     4441 atcccgagag cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc
     4501 gctctataat gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa
     4561 ggctactttg gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt
     4621 actgattact agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat
     4681 tctataccga tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct
     4741 tcattggtca gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg
     4801 aaatgtttac attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt
     4861 ttttgtctaa agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg
     4921 caagttcaag gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata
     4981 tagcaaagag atacttttga gcaatgtttg tggaagcggt attcgcaatg ggaagctcca
     5041 ccccggttga taatcagaaa agccccaaaa acaggaagat tgtataagca aatatttaaa
     5101 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     5161 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     5221 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     5281 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     5341 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     5401 gatttagagc ttgacgggga aagccctgat gaatatcttg tggtaggggt ttgggaaaat
     5461 cattcgagtt tgatgttttt cttggtattt cccactcctc ttcagagtac agaagattaa
     5521 gtgagagatc ccagtatagc gaccagcatt cacatacgat tgacgcatga tattactttc
     5581 tgcgcactta acttcgcatc tgggcagatg atgtcgaggc gaaaaaaaat ataaatcacg
     5641 ctaacatttg attaaaatag aacaactaca atataaaaaa actatacaaa tgacaagttc
     5701 ttgaaaacaa gaatcttttt attgtcagta ctgattagaa aaactcatcg agcatcaaat
     5761 gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa agccgtttct
     5821 gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt
     5881 ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa
     5941 ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagct
     6001 tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac
     6061 tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat
     6121 cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca
     6181 gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt
     6241 tgccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga
     6301 tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat
     6361 cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat
     6421 acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat
     6481 ataaatcagc atccatgttg gaatttaatc gcggcctcga aacgtgagtc ttttccttac
     6541 ccatggttgt ttatgttcgg atgtgatgtg agaactgtat cctagcaaga ttttaaaagg
     6601 aagtatatga aagaagaacc tcagtggcaa atcctaacct tttatatttc tctacagggg
     6661 cgcggcgtgg ggacaattca acgcgtctgt gaggggagcg tttccctgct cgcaggtccg
     6721 cagcgaggag ccgtaatttt tgcttcgcgc cgtgcggcca tcaaaatgta tggatgcaaa
     6781 tgattataca tggggatgta tgggctaaat gtacgggcga cagtcacatc atgcccctga
     6841 gctgcgcacg tcaagactgt caaggagggt attctgggcc tccatgtcac tagttctttg
     6901 aaaagataat gtatgattat gctttcactc atatttatac agaaacttga tgttttcttt
     6961 cgagtatata caaggtgatt acatgtacgt ttgaagtaca actctagatt ttgtagtgcc
     7021 ctcttgggct agcggtaaag gtgcgcattt tttcacaccc tacaatgttc tgttcaaaag
     7081 attttggtca aacgctgtag aagtgaaagt tggtgcgcat gtttcggcgt tcgaaacttc
     7141 tccgcagtga aagataaatg atc
//