| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-COL4A5-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001896 |
| 产品用途: | 3' AAVLINK plasmid for COL4A5 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | COL4A5 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-pCALM1-Cre-3'-COL4A5-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-pCALM1-Cre-3'-COL4A5-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-COL4A5-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-COL4A5-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-COL4A5-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-COL4A5-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-COL4A5-FLAG 6727 bp DNA circular SYN 10-AUG-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6727)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 12, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6727
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3440
/codon_start=1
/gene="COL4A5"
/gene_synonym="ASLN; ATS; ATS1; CA54"
/product="collagen alpha-5(IV) chain isoform 2 precursor"
/label=COL4A5
/note="isoform 2 precursor is encoded by transcript
variant 2; collagen of basement membrane, alpha-5 chain;
collagen IV, alpha-5 polypeptide; collagen alpha-5(IV)
chain; dA149D17.3; collagen, type IV, alpha 5; dA24A23.1"
/db_xref="CCDS:CCDS35366.1"
/db_xref="GeneID:1287"
/db_xref="HGNC:HGNC:2207"
/db_xref="MIM:303630"
/protein_id="NP_203699.1"
/translation="GVEGPPGPSGVPGQPGSPGLPGQKGDKGDPGISSIGLPGLPGPKG
EPGLPGYPGNPGIKGSVGDPGLPGLPGTPGAKGQPGLPGFPGTPGPPGPKGISGPPGNP
GLPGEPGPVGGGGHPGQPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGNPGPPGLPGLS
GQKGDGGLPGIPGNPGLPGPKGEPGFHGFPGVQGPPGPPGSPGPALEGPKGNPGPQGPP
GRPGPTGFQGLPGPEGPPGLPGNGGIKGEKGNPGQPGLPGLPGLKGDQGPPGLQGNPGR
PGLNGMKGDPGLPGVPGFPGMKGPSGVPGSAGPEGEPGLIGPPGPPGLPGPSGQSIIIK
GDAGPPGIPGQPGLKGLPGPQGPQGLPGPTGPPGDPGRNGLPGFDGAGGRKGDPGLPGQ
PGTRGLDGPPGPDGLQGPPGPPGTSSVAHGFLITRHSQTTDAPQCPQGTLQVYEGFSLL
YVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMSM
QPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPHCPQGWDSLWIGYSFMMHTSAGAE
GSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDVSDMFSKPQSETLK
AGDLRTRISRCQVCMKRT"
misc_feature 1494..1499
/label=SDS
misc_feature 1752..1757
/label=SDS
misc_feature 1779..1784
/label=SDS
misc_feature 2082..2087
/label=SDS
misc_feature 2142..2147
/label=SDS
misc_feature 2457..2462
/label=SDS
misc_feature 2642..2647
/label=SDS
misc_feature 2749..2754
/label=SDS
CDS 3447..3512
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3516..3947
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3660..3665
/label=SDS
repeat_region 3990..4130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3990..4119
/label=AAV2 ITR
repeat_region 3991..4131
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 4205..4660
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4942..5046
/gene="bla"
/label=AmpR promoter
CDS 5047..5907
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6078..6666
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca gggtgtggaa gggcctcctg
1501 gaccttctgg agttcctgga caacctggct ccccaggatt acctggacag aaaggcgaca
1561 aaggtgatcc tggtatttca agcattggtc ttccaggtct tcctggtcca aagggtgagc
1621 ctggtctgcc tggataccca gggaaccctg gtatcaaagg ttctgtggga gatcctggtt
1681 tgcccggatt accaggaacc cctggagcaa aaggacaacc aggccttcct ggattcccag
1741 gaaccccagg ccctcctgga ccaaaaggta ttagtggccc tcctgggaac cccggccttc
1801 caggagaacc tggtcctgta ggtggtggag gtcatcctgg gcaaccaggg cctccaggcg
1861 aaaaaggcaa acccggtcaa gatggtattc ctggaccagc tggacagaag ggtgaaccag
1921 gtcaaccagg ctttggaaac ccaggacccc ctggacttcc aggactttct ggccaaaagg
1981 gtgatggagg attacctggg attccaggaa atcctggcct tccaggtcca aagggcgaac
2041 caggctttca cggtttccct ggtgtgcagg gtcccccagg ccctcctggt tctccgggtc
2101 cagctctgga aggacctaaa ggcaaccctg ggccccaagg tcctcctggg agaccaggtc
2161 ctacaggttt tcaaggtcta ccaggtccag aaggtcctcc aggtctccct ggaaatggag
2221 gtattaaagg agagaaggga aatccaggcc aacctgggct acctggcttg cctggtttga
2281 aaggagatca aggaccacca ggactccagg gtaatcctgg ccggccgggt ctcaatggaa
2341 tgaaaggaga tcctggtctc cctggtgttc caggattccc aggcatgaaa ggacccagtg
2401 gagtacctgg atcagctggc cctgaggggg aaccgggact tattggtcct ccaggtcctc
2461 ctggattacc tggtccttca ggacagagta tcataattaa aggagatgct ggtcctccag
2521 gaatccctgg ccagcctggg ctaaagggtc taccaggacc ccaaggacct caaggcttac
2581 caggtccaac tggccctcca ggagatcctg gacgcaatgg actccctggc tttgatggtg
2641 caggagggcg caaaggagac ccaggtctgc caggacagcc aggtacccgt ggtttggatg
2701 gtccccctgg tccagatgga ttgcaaggtc ccccaggtcc ccctggaacc tcctctgttg
2761 cacatggatt tcttattaca cgccacagcc agacaacgga tgcaccacaa tgcccacagg
2821 gaacacttca ggtctatgaa ggcttttctc tcctgtatgt acaaggaaat aaaagagccc
2881 acggtcaaga cttggggacg gctggcagct gccttcgtcg ctttagtacc atgcctttca
2941 tgttctgcaa catcaataat gtttgcaact ttgcttcaag aaatgactat tcttactggc
3001 tctctacccc agagcccatg ccaatgagca tgcaacccct aaagggccag agcatccagc
3061 cattcattag tcgatgtgca gtatgtgaag ctccagctgt ggtgatcgca gttcacagtc
3121 agacgatcca gattccccat tgtcctcagg gatgggattc tctgtggatt ggttattcct
3181 tcatgatgca tacaagtgca ggggcagaag gctcaggtca agccctagcc tcccctggtt
3241 cctgcttgga agagtttcgt tcagctccct tcatcgaatg tcatgggagg ggtacctgta
3301 actactatgc caactcctac agcttttggc tggcaactgt agatgtgtca gacatgttca
3361 gtaaacctca gtcagaaacg ctgaaagcag gagacttgag gacacgaatt agccgatgtc
3421 aagtgtgcat gaagaggaca tgtacagact acaaagacca tgacggtgat tataaagatc
3481 atgacatcga ctacaaggat gacgatgaca agtaactcga gataatcaac ctctggatta
3541 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
3601 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
3661 ctccttgtat aaatcctggt tagttcttgc cacggcggaa ctcatcgccg cctgccttgc
3721 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt ttatttgtga
3781 aatttgtgat gctattgctt tatttgtaac catctagctt tatttgtgaa atttgtgatg
3841 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
3901 ttcattttat gtttcaggtt cagggggaga tgtgggaggt tttttaaaga tctggagacc
3961 ggtgtggcac gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc
4021 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
4081 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat
4141 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca
4201 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
4261 accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc
4321 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
4381 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
4441 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
4501 agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat
4561 ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa
4621 tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca
4681 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
4741 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
4801 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
4861 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
4921 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
4981 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
5041 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
5101 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
5161 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
5221 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
5281 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
5341 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
5401 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
5461 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
5521 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
5581 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
5641 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
5701 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
5761 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
5821 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
5881 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
5941 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
6001 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
6061 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
6121 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
6181 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
6241 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
6301 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
6361 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
6421 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
6481 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
6541 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
6601 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
6661 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
6721 cacatgt
//
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