pNCSY-mBiyu

LOCUS       pNCSY-mBiyu              3638 bp DNA     circular SYN 12-MAY-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   ustc
  TITLE     Direct Submission
  JOURNAL   Exported Sep 28, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..3638
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     20..39
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             122..144
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             152..277
                     /codon_start=1
                     /label=6xHis-tag
                     /translation="MRGSHHHHHHGMASMTGGQQMGRDLYDDDDNDPLEENLYFQG"
     CDS             164..181
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             185..217
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             257..277
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             278..1024
                     /codon_start=1
                     /label=mBiyu
                     /translation="MVSKGEEDNMASYGALLFREKIPYVVEMEGDVEGMKFSVRGKGHG
                     DANTGKIEASFICTTGELPVPWSSILTTVTYGAQCFAKYPNDIKDYLKSTMPEGYVQER
                     TITFENDGIYKTRAEVTYEKGSVYNRVTLNGSGFKKGGNILGKKLEFNFNASRTYVLPD
                     VENNGIKCYINIRHNVIGGGQIRAEHQQLNTPLGGGPVDIPHYHAIQTHTILSKDPKET
                     RDHMNVVEVFRAIDCKTAYAGMDELYK"
     terminator      1096..1143
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1240..1695
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1327..1346)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1537..1558
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1721..1825
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1826..2686
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2044..2063)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2857..3402
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
ORIGIN
        1 gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct
       61 agaatcaccg agtttattct tgacacctga tgcgatgaat gatataatag gaaagtactg
      121 ttttgtttaa ctttaagaag gagatataca tatgcggggt tctcatcatc atcatcatca
      181 tggtatggct agcatgactg gtggacagca aatgggtcgg gatctgtacg acgatgacga
      241 taacgatccg ctcgaggaaa acctgtactt ccagggtatg gtgagcaaag gcgaagaaga
      301 taacatggcg agttacggag cacttttgtt cagagaaaaa atcccctatg ttgttgagat
      361 ggaaggtgat gttgagggaa tgaagttttc agtaagaggt aaaggacatg gtgatgctaa
      421 cactggtaaa attgaagcca gtttcatctg cacaactggt gaactaccag taccttggtc
      481 gagcattcta acaactgtca catacggtgc acagtgtttt gcaaaatatc caaatgatat
      541 caaagactac ctaaaaagta ctatgccaga aggttacgtt caagagcgaa caatcacttt
      601 tgaaaacgac ggcatttata agacacgcgc tgaagtcacg tacgagaaag gatcagttta
      661 taaccgagtt acgttgaacg gttcaggatt taaaaaaggc ggtaacatct tgggcaaaaa
      721 attggaattt aacttcaacg cttcgcgcac ttatgttctt ccagatgtag aaaataatgg
      781 aatcaaatgc tacatcaaca ttcgtcataa cgtaattgga ggaggacaaa tacgagctga
      841 gcaccaacaa ttaaatactc ctcttggcgg tggaccagtc gacattccac actaccatgc
      901 gatacaaacg cacacaatac tttctaaaga tcccaaagaa acaagagatc atatgaacgt
      961 agtagaggtg ttcagagcta tcgattgcaa aaccgcatat gcaggcatgg atgaactgta
     1021 taaatgacgt ttgatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
     1081 accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
     1141 ttgctgaaag gaggaactat atccggatct ggcgtaatag cgaagaggcc cgcaccgatc
     1201 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     1261 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     1321 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     1381 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     1441 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     1501 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     1561 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     1621 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     1681 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     1741 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     1801 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     1861 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     1921 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     1981 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     2041 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     2101 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     2161 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     2221 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     2281 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     2341 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     2401 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     2461 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     2521 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     2581 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     2641 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     2701 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     2761 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     2821 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatcgttttg gtctgcgcgt
     2881 aatctcttgc tctgaaaacg aaaaaaccgc cttgcagggc ggtttttcga aggttctctg
     2941 agctaccaac tctttgaacc gaggtaactg gcttggagga gcgcagtcac caaaacttgt
     3001 cctttcagtt tagccttaac cggcgcatga cttcaagact aactcctcta aatcaattac
     3061 cagtggctgc tgccagtggt gcttttgcat gtctttccgg gttggactca agacgatagt
     3121 taccggataa ggcgcagcgg tcggactgaa cggggggttc gtgcatacag tccagcttgg
     3181 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacaaacg cggccataac
     3241 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccgcca
     3301 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccaccactg atttgagcgt
     3361 cagatttcgt gatgcttgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3421 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3481 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3541 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     3601 ccgcctctcc ccgcgcgttg gccgattcat taatgcag
//