pMal-c5x-Ectsil1-17260

LOCUS       pMal-c5x-Ectsil1-17260            7298 bp DNA     circular SYN 30-SEP-2024
DEFINITION  11_Ectsil1-17260_pMAL-c5x.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7298)
  AUTHORS   Westlake University
  TITLE     Direct Submission
  JOURNAL   Exported Sep 26, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7298
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..80
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             81..1163
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        1406..1434
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1442..1458
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1528..2628
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     CDS             2677..2688
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    2698..4377
                     /label=CDS
     terminator      4384..4470
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4562..4589
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        4617..4707
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4708..5568
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5656..6244
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6614..6805)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga
       61 gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg
      121 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa
      181 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac
      241 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc
      301 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg
      361 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc
      421 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca
      481 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga
      541 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc
      601 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg
      661 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag
      721 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga
      781 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa
      841 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatttcggta gtgggatacg
      901 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc
      961 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
     1021 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata
     1081 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     1141 cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagct cactcattag
     1201 gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg
     1261 tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg
     1321 tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt
     1381 ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga
     1441 attgtgagcg gataacaatt tcacacagga aacagccagt ccgtttaggt gttttcacga
     1501 gcaattgacc aacaaggacc atagattatg aaaatcgaag aaggtaaact ggtaatctgg
     1561 attaacggcg ataaaggcta taacggtctc gctgaagtcg gtaagaaatt cgagaaagat
     1621 accggaatta aagtcaccgt tgagcatccg gataaactgg aagagaaatt cccacaggtt
     1681 gcggcaactg gcgatggccc tgacattatc ttctgggcac acgaccgctt tggtggctac
     1741 gctcaatctg gcctgttggc tgaaatcacc ccggacaaag cgttccagga caagctgtat
     1801 ccgtttacct gggatgccgt acgttacaac ggcaagctga ttgcttaccc gatcgctgtt
     1861 gaagcgttat cgctgattta taacaaagat ctgctgccga acccgccaaa aacctgggaa
     1921 gagatcccgg cgctggataa agaactgaaa gcgaaaggta agagcgcgct gatgttcaac
     1981 ctgcaagaac cgtacttcac ctggccgctg attgctgctg acgggggtta tgcgttcaag
     2041 tatgaaaacg gcaagtacga cattaaagac gtgggcgtgg ataacgctgg cgcgaaagcg
     2101 ggtctgacct tcctggttga cctgattaaa aacaaacaca tgaatgcaga caccgattac
     2161 tccatcgcag aagctgcctt taataaaggc gaaacagcga tgaccatcaa cggcccgtgg
     2221 gcatggtcca acatcgacac cagcaaagtg aattatggtg taacggtact gccgaccttc
     2281 aagggtcaac catccaaacc gttcgttggc gtgctgagcg caggtattaa cgccgccagt
     2341 ccgaacaaag agctggcaaa agagttcctc gaaaactatc tgctgactga tgaaggtctg
     2401 gaagcggtta ataaagacaa accgctgggt gccgtagcgc tgaagtctta cgaggaagag
     2461 ttggtgaaag atccgcgtat tgccgccact atggaaaacg cccagaaagg tgaaatcatg
     2521 ccgaacatcc cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt gatcaacgcc
     2581 gccagcggtc gtcagactgt cgatgaagcc ctgaaagacg cgcagactaa ttcgagctcg
     2641 aacaacaaca acaataacaa taacaacaac ctcgggatcg agggaaggat ttcacatatg
     2701 acactacagg tacaagaagg aagttcagct gcggcggccg aagcgttggc ggcgcgaagc
     2761 gcagctgcgt ctgcggtcgc caaaccgttc gtgtcccgca agctgggttc ctttgaaaag
     2821 atgttgacca aaactcgtga tggcgcaggc ccagctgagg atggcgttcg taccctgacc
     2881 accccgcatg tttgggccgc tgtgatcgac ggcgacctgc cgcgtagcag cctcaagcgt
     2941 gggatcgaag cggtgctggc acgccacccg atgctgcgtg cctgcatccg cacgccggat
     3001 ggcccaaaag aaccgctgat taacattctg ggtgaagagc gggaggacgg cgacccgctg
     3061 tatttttgcg aaagtgaatg tgagagcctg ggagcgctgg ccgaacgcgt cctggctccg
     3121 gaagaggacg agatcagcgg cgacgaagcc tttacccgtg agtggcgtgg tcgtctggag
     3181 cgcaacttag ataacgcacg cttaccggtt agcgcgggcc cgaattggcg tgtggaagcc
     3241 atccgtctgt ccggtagagg tcgcccaggt cctcgtcgcc gccgtacggc tcttgtgtgt
     3301 accatgaatc atgcgctgga ggaccaacgt agcagcaacg tgatgctgca aggtatcctg
     3361 gaggcggctg cgggtaaagc aagcggtccg gcccgttctg cggatgatgc aggcggcttc
     3421 gacctgcctc cgagcatgga agcggcgatt gttgagggaa agcagttccg tcgtaacacc
     3481 ctgggttata tgtgggatca ggctaccgtt gcgattgcac agccgatggt cgtgccggat
     3541 ggcctgccgt ctgtggacga gcgtagcgaa ggtggtgacg aaggcccgtt cggcgtttcc
     3601 agcagaaaga gcgcgtgcga gttcagcagc gttccggctg acgacgtatc ggttatgctg
     3661 caggcgtgcc gtgaacgcgg tctgacgctg tccggcgcgt tgagtgcggc gtgcctgatg
     3721 gcatgcagcg acgttgcgca cgcggagggc tcaagaggct ctaaccgtta caaattcttg
     3781 ttggcggttg atctgcggcg ttttggcact ggtgactaca gcgatatgga tgactggacc
     3841 ggtggcacgg ttgcttgcgc aggtggtgca atagactacg cagttaaagt tccggctggc
     3901 agcggttcac gcctggtggg tcgtgatggt gaggaggctg cccgcgtggc gcgtgaagaa
     3961 ttctggcgat tggcggaacg ttgtaaaacg gcgacccgtg atatggtcga gaaagagaca
     4021 ctgcgtgagg ccgtggcggt atttgactgg gcaatggaga acatgagcat ttgggcaagc
     4081 gtggatatcg agagccgcaa tgccaagacc cttgggcgtg cgtacacctg tggtgtttcc
     4141 aacatgggtc gttacccgtt cgatacccag gtcggtaatc tgtcgcttaa ggcagtgcac
     4201 tatggtacga gccaaagcgc atgcggttcc ctgtttcagc tctcttgtgg caccattgac
     4261 ggcgaattgt tcatgaccct gcaatttgca gagccggtgg tcacccgcga ggctggcgaa
     4321 gcgtatttgc gtggtatcat tgataatctc cgcgcggcct gcgttatctc gcaataaaag
     4381 cttcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     4441 tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag
     4501 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaattaag
     4561 cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactctttc ggtccgttgt
     4621 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     4681 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     4741 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     4801 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     4861 ggtaagatcc ttgagagttt tcgccccgaa gaacgtttcc caatgatgag cacttttaaa
     4921 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     4981 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     5041 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     5101 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     5161 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     5221 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     5281 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     5341 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     5401 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     5461 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     5521 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     5581 gtttactcat atatacttta gattgatttc cttaggactg agcgtcaacc ccgtagaaaa
     5641 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     5701 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     5761 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     5821 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     5881 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     5941 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     6001 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     6061 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     6121 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     6181 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     6241 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     6301 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     6361 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     6421 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     6481 ataaggtgca ctgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg
     6541 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     6601 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt
     6661 aaagctcatc agcgtggtcg tgcagcgatt cacagatgtc tgcctgttca tccgcgtcca
     6721 gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa
     6781 gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg
     6841 gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac
     6901 atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc
     6961 agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac
     7021 agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact
     7081 tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg
     7141 tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct
     7201 gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca
     7261 tgcgcacccg tggccaggac ccaacgctgc ccgaaatt
//