| 质粒名称: | pAAVLINK-EF1a-HA-CHD7-A |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001731 |
| 产品用途: | 3' AAVLINK plasmid for CHD7 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | CHD7 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-CHD7-A
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-CHD7-A载体质粒基本信息
pAAVLINK-EF1a-HA-CHD7-A质粒图谱载体图谱和pAAVLINK-EF1a-HA-CHD7-A载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-CHD7-A质粒载体简介
pAAVLINK-EF1a-HA-CHD7-A质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-CHD7-A 7112 bp DNA circular SYN 21-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7112)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 4, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7112
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 146..688
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 175..386
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 399..667
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 493..498
/label=SDS
CDS 704..730
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 737..4252
/codon_start=1
/label=CHD7
/translation="ADPGMMSLFGEDGNIFSEGLEGLGECGYPENPVNPMGQQMPIDQG
FASLQPSLHHPSTNQNQTKLTHFDHYNQYEQQKMHLMDQPNRMMSNTPGNGLASPHSQY
HTPPVPQVPHGGSGGGQMGVYPGMQNERHGQSFVDSSSMWGPRAVQVPDQIRAPYQQQQ
PQPQPPQPAPSGPPAQGHPQHMQQMGSYMARGDFSMQQHGQPQQRMSQFSQGQEGLNQG
NPFIATSGPGHLSHVPQQSPSMAPSLRHSVQQFHHHPSTALHGESVAHSPRFSPNPPQQ
GAVRPQTLNFSSRSQTVPSPTINNSGQYSRYPYSNLNQGLVNNTGMNQNLGLTNNTPMN
QSVPRYPNAVGFPSNSGQGLMHQQPIHPSGSLNQMNTQTMHPSQPQGTYASPPPMSPMK
AMSNPAGTPPPQVRPGSAGIPMEVGSYPNMPHPQPSHQPPGAMGIGQRNMGPRNMQQSR
PFIGMSSAPRELTGHMRPNGCPGVGLGDPQAIQERLIPGQQHPGQQPSFQQLPTCPPLQ
PHPGLHHQSSPPHPHHQPWAQLHPSPQNTPQKVPVHQHSPSEPFLEKPVPDMTQVSGPN
AQLVKSDDYLPSIEQQPQQKKKKKKNNHIVAEDPSKGFGKDDFPGGVDNQELNRNSLDG
SQEEKKKKKRSKAKKDPKEPKEPKEKKEPKEPKTPKAPKIPKEPKEKKAKTATPKPKSS
KKSSNKKPDSEASALKKKVNKGKTEGSENSDLDKTPPPSPPPEEDEDPGVQKRRSSRQV
KRKRYTEDLEFKISDEEADDADAAGRDSPSNTSQSEQQESVDAEGPVVEKIMSSRSVKK
QESGEEVEIEEFYVKYKNFSYLHCQWASIEDLEKDKRIQQKIKRFKAKQGQNKFLSEIE
DELFNPDYVEVDRIMDFARSTDDRGEPVTHYLVKWCSLPYEDSTWERRQDIDQAKIEEF
EKLMSREPETERVERPPADDWKKSESSREYKNNNKLREYQLEGVNWLLFNWYNMRNCIL
ADEMGLGKTIQSITFLYEIYLKGIHGPFLVIAPLSTIPNWEREFRTWTELNVVVYHGSQ
ASRRTIQLYEMYFKDPQGRVIKGSYKFHAIITTFEMILTDCPELRNIPWRCVVIDEAHR
LKNRNCKLLEGLKMMDLEHKVLLTGTPLQNTVEELFSLLHFLEPSRFPSETTFMQEFGD
LKTEEQ"
regulatory 1075..1084
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
misc_feature 1958..1963
/label=SDS
misc_feature 2936..2941
/label=SDS
misc_feature 2939..2944
/label=SDS
misc_feature 3042..3047
/label=SDS
misc_feature 3186..3191
/label=SDS
misc_feature 3572..3577
/label=SDS
intron 4253..4293
/label=Splicing Donor
misc_feature 4297..4330
/label=loxP-JT1571
repeat_region 4375..4515
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4375..4504
/label=AAV2 ITR
rep_origin 4590..5045
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5327..5431
/gene="bla"
/label=AmpR promoter
CDS 5432..6292
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6463..7051
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
721 tgactatgct ggatctgcag atccaggaat gatgagtctt tttggcgagg atgggaatat
781 tttcagtgaa ggtcttgaag gcctcggaga atgtggttac ccggaaaatc cagtaaatcc
841 tatgggtcag caaatgccaa tagaccaagg ctttgcctct ttacagccat cccttcatca
901 tccttcaact aatcaaaatc aaacaaagct gacacatttt gatcactata atcagtatga
961 acaacaaaag atgcatctga tggatcagcc gaacagaatg atgagcaaca cccctgggaa
1021 cggactcgcg tctccgcact cgcagtatca cacccctccc gttcctcagg tgccccatgg
1081 tggcagtggt ggcggtcaga tgggtgtcta ccctggcatg cagaatgaga ggcatgggca
1141 atcctttgtg gacagcagct ccatgtgggg ccccagggct gttcaggtac cagaccagat
1201 acgagccccc taccagcagc agcagccaca gccgcagcca ccgcagccgg ctccgtcggg
1261 gccccctgca cagggccacc ctcagcacat gcagcagatg ggcagctata tggcacgtgg
1321 ggatttttcc atgcagcagc atggtcagcc acagcagagg atgagccagt tttcccaagg
1381 ccaagagggc ctcaatcagg gaaatccttt tattgccacc tcaggacctg gccacttgtc
1441 ccacgtgccc cagcagagtc ccagcatggc accttccttg cgtcactcgg tgcagcagtt
1501 ccatcaccac ccctctactg ctctccatgg agaatccgtt gcccacagtc ccagattctc
1561 cccgaatcct ccccaacaag gggctgttag gccgcaaacc cttaacttta gttctcggag
1621 ccagacagtc ccctctccta ctataaacaa ctcagggcag tattctcgat atccttacag
1681 taacctaaat cagggattag ttaacaatac agggatgaat caaaatttag gccttacaaa
1741 taatactcca atgaatcagt ccgtaccaag ataccccaat gctgtaggat tcccatcaaa
1801 cagtggtcaa ggactaatgc accagcagcc catccacccc agtggctcac ttaaccaaat
1861 gaacacacaa actatgcatc cttcacagcc tcagggaact tatgcctctc cacctcccat
1921 gtcacccatg aaagcaatga gtaatccagc aggcactcct cctccacaag tcaggccggg
1981 aagtgctggg ataccaatgg aagttggcag ttatccaaat atgccccatc ctcagccatc
2041 tcaccagccc cctggtgcca tgggaatcgg acagaggaat atgggcccca gaaacatgca
2101 gcagtctcgt ccatttatag gcatgtcctc ggcaccaagg gaattgactg ggcacatgag
2161 gccaaatggt tgtcctggtg ttggccttgg agacccacaa gcaatccagg aacgactgat
2221 acctggccaa caacatcctg gtcaacagcc atcttttcag cagttgccaa cctgtcctcc
2281 actgcagcct cacccgggct tgcaccacca gtcttcacct ccacaccctc atcaccagcc
2341 ttgggcacag ctccacccat caccccagaa caccccgcag aaagtgcctg tgcatcagca
2401 ttccccgtcg gagccctttc tagagaaacc agtgccggat atgactcagg ttagtggacc
2461 gaatgctcag ctagtgaaga gtgatgatta cctgccatca atagaacagc agccacaaca
2521 aaagaagaag aaaaagaaaa acaaccacat tgtagcagag gatcccagta aaggttttgg
2581 taaagatgac ttccctggtg gggtagataa ccaagaacta aataggaact cactggatgg
2641 gtcccaagaa gaaaaaaaga aaaagaaaag gtcaaaggca aaaaaagacc cgaaggaacc
2701 gaaagaaccc aaggagaaaa aagagcccaa ggaacccaag accccgaaag cccctaagat
2761 tcccaaagag ccaaaggaaa agaaagcaaa aactgccacg ccaaaaccca aatccagcaa
2821 aaagtcaagt aataagaaac ctgactcaga agcaagtgct ttgaagaaaa aggtcaacaa
2881 gggaaaaaca gaaggttctg aaaattcaga cttagacaaa acacccccac catctcctcc
2941 tcctgaagaa gatgaggacc caggtgttca gaagagacgg tccagcagac aggtgaagag
3001 aaagcgctac actgaagacc tggagttcaa gatttctgat gaggaggcag atgatgcaga
3061 tgctgctggg agggattccc cctccaacac ctcccagtca gaacagcagg aatctgttga
3121 tgcagaaggc ccagtggtag aaaaaattat gagcagtcgt tcagtaaaaa agcaggaatc
3181 tggagaggag gtagaaattg aggaattcta tgtgaaatac aaaaacttct cttatcttca
3241 ttgtcagtgg gcatctatag aagatctgga aaaagataag agaattcagc aaaaaattaa
3301 acgatttaag gcaaagcagg gccagaacaa gttcctttca gagattgagg atgagctttt
3361 taatccagat tatgtggagg ttgaccggat aatggacttt gcacgtagca cagatgaccg
3421 gggagagcct gtgactcact atctggtgaa gtggtgttca cttccttatg aagacagcac
3481 gtgggagcgg aggcaggaca tagatcaagc aaagatcgag gagtttgaga aactaatgtc
3541 cagggagccg gaaacagagc gtgtggagcg acctcctgct gatgattgga agaaatcgga
3601 gagttccagg gagtataaaa acaataacaa actcagggaa taccagttgg agggagtaaa
3661 ctggctactt ttcaattggt acaacatgcg aaactgcatt ttagcagatg aaatgggttt
3721 gggaaaaact atccagtcca ttacatttct ctatgagata tatttgaaag gaatccatgg
3781 ccctttttta gtaattgccc cattgtccac aatccccaac tgggaaaggg aattccgaac
3841 ctggacagag ttgaacgtgg ttgtgtatca tgggagtcaa gctagtcgtc ggaccattca
3901 gttgtatgaa atgtacttca aagatcccca gggtcgagtg ataaaggggt cctataagtt
3961 tcatgccatc atcactacat ttgagatgat tttgactgat tgtcctgagc tgcggaatat
4021 tccatggcgc tgtgtagtca ttgatgaagc ccacaggctg aagaacagga actgcaagct
4081 gttggaggga ctcaagatga tggacttgga acacaaagtg ctgctgacgg gaaccccact
4141 ccagaacact gtggaagaac tcttcagctt gcttcatttc ttggaaccaa gtcgcttccc
4201 ttcagaaacc acatttatgc aagaatttgg tgatctaaaa acagaagagc aggtaagtat
4261 caaggttaca agacaggttt aaggagacca ataagctatt attcgtatag catacattat
4321 acgaagttat taagttatgg agaccggtgt ggcacgtgcg gaccgagcgg ccgcaggaac
4381 ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc
4441 gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc
4501 gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
4561 cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc
4621 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc
4681 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
4741 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
4801 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
4861 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
4921 ctctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg tctattggtt
4981 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
5041 aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
5101 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
5161 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
5221 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
5281 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
5341 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
5401 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
5461 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
5521 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
5581 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
5641 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
5701 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
5761 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
5821 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
5881 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
5941 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
6001 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
6061 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
6121 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
6181 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
6241 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
6301 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
6361 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
6421 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
6481 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
6541 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
6601 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
6661 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
6721 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
6781 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
6841 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
6901 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
6961 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
7021 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
7081 cctggccttt tgctggcctt ttgctcacat gt
//
