| 质粒名称: | pAAVLINK-CHD9-B |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001740 |
| 产品用途: | Body' AAVLINK plasmid for CHD9 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | Promoterless |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | CHD9 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-CHD9-B
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-CHD9-B载体质粒基本信息
pAAVLINK-CHD9-B质粒图谱载体图谱和pAAVLINK-CHD9-B载体序列质粒序列多克隆位点信息
pAAVLINK-CHD9-B质粒载体简介
pAAVLINK-CHD9-B质粒序列载体序列
LOCUS pAAVLINK-CHD9-B 6362 bp DNA circular SYN 22-JUL-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6362)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6362
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..141
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..141
/label=AAV2 ITR
protein_bind 156..189
/label=loxJTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 190..281
/label=Splicing Acceptor
/note="Splicing acceptor"
misc_feature 282..3518
/label=CHD9
intron 3519..3559
/label=Splicing Donor
misc_feature 3563..3596
/label=lox2272-JT1571
repeat_region 3625..3765
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3625..3754
/label=AAV2 ITR
rep_origin 3840..4295
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4577..4681
/gene="bla"
/label=AmpR promoter
CDS 4682..5542
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5713..6301
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
121 actccatcac taggggttcc tgcggccgca cgcgtataac ttcgtatagc atacattata
181 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
241 tattggtctt actgacatcc actttgcctt tctctccaca ggtttccttc tgaatcaaca
301 tttatgcaag aatttgggga tctgaaaaca gaggaacagg tacagaaact tcaggctatc
361 ctgaaaccaa tgatgttgag acgattaaaa gaagatgtgg aaaagaagtt ggcacctaaa
421 gaagaaacca tcattgaagt agaacttact aatattcaaa agaaatacta ccgggctatc
481 ttggaaaaga acttttcttt tttatccaaa ggagcaggac aaactaatgt acctaacttg
541 gtcaatacca tgatggagct caggaaatgt tgtaatcatc catatcttat aaaaggtgct
601 gaggagaaaa tacttggaga atttagagat acttacaatc cagctgcttc tgattttcat
661 cttcaagcaa tgatccagtc tgctggtaaa ttggtcctta ttgataaatt gcttcccaaa
721 atgaaagccg gaggtcataa agtgctcatc ttctctcaaa tggttcgttg ccttgacatt
781 ctggaggact atctcataca taaaagatac ttatatgagc gaattgatgg cagagtcaga
841 ggaaatcttc ggcaagctgc tatagataga tttagtaaac ctgattcaga tagatttgtt
901 tttctcctgt gtacccgagc tggtgggttg ggcatcaact taactgcagc tgatacatgt
961 ataatttttg attctgattg gaatcctcag aatgatcttc aggcccaagc tcgttgccac
1021 agaattggtc agaacaaagc agttaaagtc tacagactgg taactcgtaa ctcatatgag
1081 agagagatgt ttgaccgagc cagtttgaaa ctgggcctag ataaagctgt gttacagagc
1141 atgagtggaa gagaaagtaa tgttggtggt attcagcagc tttccaaaaa ggaaatagaa
1201 gatctgcttc gaagaggtgc ttatggtgct attatggagg aagaagatga aggctctaaa
1261 ttctgcgaag aggatatcga tcagatttta ctacgtcgta caaaaactat tacaattgaa
1321 tcagaaggac gtgggtcaac atttgccaag gcgagttttg tggcatctgg aaaccggaca
1381 gatatttctt tagatgatcc caacttctgg caaaaatggg ctaaaaaggc agaaatagat
1441 atagaggcca tcagtggcag aaacagcttg gttattgaca ctccaagaat taggaagcaa
1501 acaagacctt ttagtgccac aaaagatgaa ttggctgaat tatctgaagc tgaaagtgaa
1561 ggagatgaaa agcccaaact ccggagaccc tgtgaccgtt ccaatggcta tggaagaact
1621 gaatgcttta gagttgagaa aaacctgcta gtttatgggt ggggccgatg gagagagatt
1681 ctatctcatg gccgtttcaa aaggcagcta aatgaacacg atgtagagat aatttgccga
1741 gctctcttag catattgcct tgttcactac cgaggagatg agaagattaa aggtttcata
1801 tgggatctca ttactccaac tgaagatgga cagacacgag agctacagaa tcatctaggc
1861 ctatcagctc ctgtacccag gggtcgaaaa gggaagaaag taaaaactca aacaagctca
1921 tttgatatac aaaaagcaga atggcttcga aaatataatc ccgagcagct ccttcaagat
1981 gaaggctaca aaaaacatat aaaacaccac tgtaataagg ttttgcttcg tgtgagaatg
2041 ctgtattatc taaagcaaga agttattgga aatgagtgtc agaaagtatt tgatggagtt
2101 gatgcaagtg acattgatgt ttgggtacca gaaccagacc actcagaagt tcctgctgag
2161 tggtgggatt ttgatgctga taagtcactc cttattggag tttttaaaca tggatatgaa
2221 aaatataaca ctattcgagc agacccagca ttatgcttct tggaaagagt gggaaaacct
2281 gatgagaaag cagttgctgc tgaacagaga gcgaatgatt atatggatgg ggatgtggaa
2341 gatccagaat acaaacctgc cccagccatc tttaaagatg atatagagga tgatgtttcc
2401 tcaccaggag atcttgttat agcagatgga gatggtcaac tgatggaggg tgataaagta
2461 tattggccta ctcaatcagc tttaaccaca cgtttgaggc gtctcatcac tgcataccag
2521 cgtactaata aaaacagaca aattcagcag atacaaccga ctttctcggt gcctaccagt
2581 gtaatgcagc ctatttatga ggaagccact cttaatccta aaatggcagc caagatagaa
2641 agacagcaaa gatggacaag aagagaagaa gctgactttt atagggttgt atctacattt
2701 ggagtggttt ttgaccctga cagaggccaa tttgattgga caaaatttag agctatggct
2761 aggctacata agaaaactga tgatagtttg gaaaaatatt tgtacgcatt catgtccatg
2821 tgtcggaggg tttgtcgtct tccttccaaa gaagaattgg tggatccaaa tatttttatc
2881 cagcccatca cagaagaacg tgcttctagg actttgtatc gcattgaact tctaaggaaa
2941 gtacgggaac aggcccttcg acatccacag ttgtttgaac gcttgaagct ttgccatcca
3001 aatccagatt taccagtctg gtgggaatgt ggccctcatg atagggattt gcttattggt
3061 gctgccaaac acggggtgag ccgaacagac tatcacattc ttcgtgatcc tgaactctca
3121 tttatggcag ctcagaggaa ctacagtcaa agtaagatgg ctcattcaag gacttctacc
3181 ccacttctac agcaatatca agtagcactt tctgcttctc ctcttacctc tctacctagg
3241 ctcctagatg ctaaaggtat tattctagag gagatgaaag ttaaaagtga aaaccttaaa
3301 gaggagcctc agtcttctga agaagaatct atgtcttctg tggaaaccag gacactaata
3361 aaatctgagc ctgtaagtcc aaagaatggt gttttaccac aggctactgg agaccagaaa
3421 tctggtggaa aatgtgaaac agacagacgc atggttgcag ccagaacaga acccctaact
3481 ccaaacccag cttctaagaa accaagagtc cacaaaaggt aagtatcaag gttacaagac
3541 aggtttaagg agaccaataa gctattattc gtataggata ctttatacga agttatgaat
3601 tccacgtgcg gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc
3661 tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg
3721 cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt
3781 attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta
3841 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
3901 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
3961 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
4021 tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc
4081 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
4141 actcttgttc caaactggaa caacactcaa ccctatctcg ggctattctt ttgatttata
4201 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
4261 cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg
4321 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
4381 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
4441 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
4501 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
4561 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
4621 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
4681 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
4741 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
4801 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
4861 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
4921 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
4981 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
5041 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
5101 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
5161 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
5221 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
5281 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
5341 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
5401 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
5461 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
5521 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
5581 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
5641 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
5701 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
5761 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
5821 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
5881 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
5941 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
6001 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
6061 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
6121 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
6181 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
6241 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
6301 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
6361 gt
//
