pCAGGS-RSV-P(A2)

LOCUS       pCAGGS-RSV-P(A2)              5557 bp DNA     circular SYN 16-JUL-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Westlake University
  TITLE     Direct Submission
  JOURNAL   Exported Aug 27, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..5557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..385
                     /label=CMV
                     /note="/vntifkey=30"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..737
                     /label=Chicken beta-actin promoter
                     /label=Chicken\beta-actin\promoter
                     /note="/vntifkey=29"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     intron          738..1629
                     /label=intron
                     /note="/vntifkey=15"
     primer_bind     1680..1697
                     /label=JL5 pACGGS vector F
     misc_feature    1741..1749
                     /label=Kozak sequence consensus
     CDS             1750..2475
                     /codon_start=1
                     /label=P
                     /translation="MEKFAPEFHGEDANNRATKFLESIKGKFTSPKDPKKKDSIISVNS
                     IDIEVTKESPITSNSTIINPTNETDDTAGNKPNYQRKPLVSFKEDPTPSDNPFSKLYKE
                     TIETFDNNEEESSYSYEEINDQTNDNITARLDRIDEKLSEILGMLHTLVVASAGPTSAR
                     DGIRDAMIGLREEMIEKIRTEALMTNDRLEAMARLRNEESEKMAKDTSDEVSLNPTSEK
                     LNNLLEGNDSDNDLSLEDF"
     primer_bind     1750..1773
                     /label=RSV-P-F
     primer_bind     complement(2447..2475)
                     /label=RSV-P-R
     primer_bind     complement(2473..2495)
                     /label=Primer 1
     polyA_signal    2497..3037
                     /label=Rabbit beta-globin poly-A
                     /label=Rabbit\beta-globin\poly-A
                     /note="/vntifkey=25"
     primer_bind     2497..2516
                     /label=pCAGGS-F
     primer_bind     complement(2524..2544)
                     /label=JY412
     polyA_signal    2644..2699
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3021..3037)
                     /label=pCAGGS-R
     primer_bind     complement(3060..3076)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3084..3100
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3108..3138)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3153..3174
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    3228..3564
                     /label=SV40
                     /note="/vntifkey=21"
     promoter        3233..3428
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3279..3414
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3434..3568
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3807..4395)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4566..5426)
                     /codon_start=1
                     /label=Amp
                     /note="/vntifkey=4"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5427..5531)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcgcggccgc
     1741 gccgccacca tggaaaagtt tgctcctgaa ttccatggag aagatgcaaa caacagggct
     1801 actaaattcc tagaatcaat aaagggcaaa ttcacatcac ccaaagatcc caagaaaaaa
     1861 gatagtatca tatctgtcaa ctcaatagat atagaagtaa ccaaagaaag ccctataaca
     1921 tcaaattcaa ctattatcaa cccaacaaat gagacagatg atactgcagg gaacaagccc
     1981 aattatcaaa gaaaacctct agtaagtttc aaagaagacc ctacaccaag tgataatccc
     2041 ttttctaaac tatacaaaga aaccatagaa acatttgata acaatgaaga agaatccagc
     2101 tattcatacg aagaaataaa tgatcagaca aacgataata taacagcaag attagatagg
     2161 attgatgaaa aattaagtga aatactagga atgcttcaca cattagtagt ggcaagtgca
     2221 ggacctacat ctgctcggga tggtataaga gatgccatga ttggtttaag agaagaaatg
     2281 atagaaaaaa tcagaactga agcattaatg accaatgaca gattagaagc tatggcaaga
     2341 ctcaggaatg aggaaagtga aaagatggca aaagacacat cagatgaagt gtctctcaat
     2401 ccaacatcag agaaattgaa caacctattg gaagggaatg atagtgacaa tgatctatca
     2461 cttgaagatt tctgataggt accatcgata ccggtgaatt cactcctcag gtgcaggctg
     2521 cctatcagaa ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc
     2581 tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg
     2641 gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac
     2701 tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga
     2761 gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc
     2821 atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt
     2881 gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat
     2941 tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag
     3001 ctgtccctct tctcttatgg agatccctcg acctgcagcc caagcttggc gtaatcatgg
     3061 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     3121 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     3181 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ccgcatctca
     3241 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     3301 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     3361 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     3421 tttgcaaaaa gctaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     3481 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     3541 catcaatgta tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg
     3601 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     3661 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     3721 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     3781 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     3841 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     3901 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     3961 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     4021 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     4081 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     4141 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     4201 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     4261 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     4321 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     4381 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     4441 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     4501 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     4561 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     4621 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     4681 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     4741 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     4801 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     4861 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     4921 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     4981 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     5041 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     5101 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     5161 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     5221 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     5281 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     5341 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     5401 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     5461 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     5521 ggggttccgc gcacatttcc ccgaaaagtg ccacctg
//
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