pH2B-mScarlet-I

LOCUS       pH2B-mScarlet-I            5023 bp DNA     circular SYN 19-AUG-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 10, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..5023
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          990..1685
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             597..974
                     /codon_start=1
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAVTKYTSSK"
     primer_bind     988..1006
                     /label=mScarlet-up
     CDS             990..1685
                     /codon_start=1
                     /label=mScarlet-I
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(1398..1427)
                     /label=mS-S147X RE
     primer_bind     1413..1442
                     /label=mS-S147X UP
     primer_bind     1413..1442
                     /label=mS-S147AE
     primer_bind     1413..1442
                     /label=mS-S147T
     primer_bind     1413..1442
                     /label=mS-S147PQ
     primer_bind     1413..1442
                     /label=147Q-U
     primer_bind     complement(1435..1463)
                     /label=K159Y RE
     primer_bind     complement(1449..1478)
                     /label=M164DE RE
     primer_bind     1449..1478
                     /label=K159Y UP
     primer_bind     1464..1493
                     /label=M164DE up
     primer_bind     1464..1493
                     /label=M164N UP
     primer_bind     1464..1493
                     /label=M164Q UP
     primer_bind     1464..1493
                     
     primer_bind     1464..1493
                     
     primer_bind     1464..1493
                     /label=M164ATP
     primer_bind     1464..1493
                     /label=M164WY
     primer_bind     1464..1493
                     /label=　mSclt-M164A
     primer_bind     1464..1493
                     /label=mSclt-M164Y
     primer_bind     1464..1493
                     /label=mSclt-M164H up
     primer_bind     complement(1663..1688)
                     /label=mScarlet-down
     primer_bind     complement(1665..1696)
                     /label=0907-1040-R
     polyA_signal    1811..1932
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1939..2394)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2421..2525
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2527..2884
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2735..2870
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2919..3713
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3945..3992
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4321..4909
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcatgc
      601 ctgaaccggc aaaatccgct ccggccccta aaaagggctc caagaaagcc gtcaccaaag
      661 cccagaagaa agacggcaag aagcgcaagc gcagccgcaa agagagctac tccatctacg
      721 tgtacaaggt gctgaagcag gtccaccccg acaccggcat ctcgtccaag gccatgggca
      781 tcatgaactc cttcgtcaac gacatcttcg agcgcatcgc gggagaggct tcccgcctgg
      841 cgcactacaa caagcgctcc accatcacat cccgcgagat ccagacggcc gtgcgcctgc
      901 tgctgcccgg cgagctggcc aagcacgccg tgtccgaggg caccaaggcg gtcaccaagt
      961 acaccagctc caagccaccg gtcgccacca tggtgagcaa gggagaggcc gtgatcaagg
     1021 agttcatgag attcaaggtg cacatggagg gaagcatgaa cggacacgag ttcgagatcg
     1081 agggcgaggg cgagggaagg ccatacgagg ggacccagac agcaaagctg aaggtgacaa
     1141 agggcggacc cctgcctttt agctgggaca tcctgagccc acaatttatg tatggcagca
     1201 gagcctttat taagcacccc gccgacatcc ccgactacta caaacaatcc tttcccgagg
     1261 gctttaaatg ggaacgggtg atgaacttcg aagacggcgg cgccgtgacc gtgacccagg
     1321 ataccagcct ggaagacgga accctgatct acaaagtgaa gctcagaggc accaacttcc
     1381 cccccgacgg ccccgttatg cagaagaaga caatgggttg ggaggccagc accgagagac
     1441 tctaccccga ggacggcgtg ctcaagggcg acattaagat ggcactgcgc ctgaaggacg
     1501 gcggaagata cctggccgac ttcaagacca cctacaaggc caaaaagccc gtgcagatgc
     1561 ccggcgcata caacgtggac agaaagctgg acataaccag ccacaacgaa gactacaccg
     1621 tggtggagca atacgagaga agcgaaggaa ggcacagcac aggaggcatg gatgagctgt
     1681 ataaataaag cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt
     1741 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     1801 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     1861 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     1921 caatgtatct taaggcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     1981 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     2041 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     2101 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     2161 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     2221 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     2281 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     2341 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg
     2401 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     2461 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     2521 agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc
     2581 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     2641 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     2701 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     2761 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     2821 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     2881 caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     2941 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     3001 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     3061 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat
     3121 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     3181 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     3241 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     3301 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     3361 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     3421 ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc
     3481 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     3541 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     3601 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     3661 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     3721 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     3781 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     3841 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac
     3901 tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca
     3961 gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg
     4021 gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt
     4081 ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc
     4141 ggcaggccct gccatagcct caggttactc atatatactt tagattgatt taaaacttca
     4201 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     4261 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     4321 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     4381 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     4441 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
     4501 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     4561 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4621 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     4681 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     4741 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4801 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     4861 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     4921 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     4981 gttatcccct gattctgtgg ataaccgtat taccgccatg cat
//