| 质粒名称: | pYK262 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000809 |
| 产品用途: | Mammalian PhCMV-driven constitutive SEAP expression vector containing 14 tandem TetR-specific tetO repeats in the 5’-promoter region (tetO14-PhCMV-SEAP-pA). |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | tetO14-SEAP |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Programmable solid-state condensates for spatiotemporal control of mammalian gene expression
Wang Y., Jiang J., Xiong Q., Li S., Shao J., Xie M., Zeng A.P.
Nat. Chem. Biol. 2025 1–10 (2025)
|
pYK262
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pYK262载体质粒基本信息
pYK262质粒图谱载体图谱和pYK262载体序列质粒序列多克隆位点信息
pYK262质粒载体简介
pYK262质粒序列载体序列
LOCUS pYK262 7616 bp DNA circular SYN 11-FEB-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7616)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 27, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7616
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(111..135)
/label=oYK262_V_R
primer_bind 116..162
/label=oYK262_tet_F
primer_bind 116..137
/label=oYK340_TET-F
primer_bind 135..145
/label=oYK244_tetO7_F
primer_bind 135..144
/label=oYW05
misc_feature 151..444
/label=TET07
protein_bind 164..434
/label=tetracycline response element
/bound_moiety="tetracycline repressor TetR"
/note="contains seven copies of the tetracycline operator
tetO"
primer_bind complement(168..187)
/label=sgRNA to tetR
primer_bind complement(210..229)
/label=sgRNA to tetR
primer_bind complement(252..271)
/label=sgRNA to tetR
primer_bind complement(294..313)
/label=sgRNA to tetR
primer_bind complement(336..355)
/label=sgRNA to tetR
primer_bind complement(378..397)
/label=sgRNA to tetR
primer_bind complement(420..439)
/label=sgRNA to tetR
primer_bind complement(440..479)
/label=oYK262_tet_R
primer_bind complement(440..460)
/label=oYK244_tetO7_R
primer_bind complement(440..459)
/label=oYW06
misc_feature 445..460
/label=hcmvmin
primer_bind 460..502
/label=oYK244_tetO7_F
primer_bind 482..501
/label=oYW05
primer_bind 492..519
/label=oYK262_tet_F
misc_feature 508..801
/label=TET07
protein_bind 521..791
/label=tetracycline response element
/bound_moiety="tetracycline repressor TetR"
/note="contains seven copies of the tetracycline operator
tetO"
primer_bind complement(525..544)
/label=sgRNA to tetR
primer_bind complement(567..586)
/label=sgRNA to tetR
primer_bind complement(609..628)
/label=sgRNA to tetR
primer_bind complement(651..670)
/label=sgRNA to tetR
primer_bind complement(693..712)
/label=sgRNA to tetR
primer_bind complement(735..754)
/label=sgRNA to tetR
primer_bind complement(777..796)
/label=sgRNA to tetR
primer_bind complement(797..837)
/label=oYK244_tetO7_R
primer_bind complement(797..817)
/label=oYK262_tet_R
primer_bind complement(797..816)
/label=oYW06
misc_feature 802..817
/label=hcmvmin
primer_bind complement(819..840)
/label=oYK340_TET-R
misc_feature 841..1428
/label=CMV
primer_bind complement(881..904)
/label=262-R
primer_bind 1472..1491
/label=T7
primer_bind 1490..1526
/label=oYK55-SEAP-F
primer_bind 1490..1509
/label=oYK06-ANR-mEGFP-F
primer_bind 1509..1536
/label=oDJ273-1
misc_feature 1510..3069
/label=SEAP
primer_bind complement(3048..3069)
/label=oDJ273-2
primer_bind complement(3050..3115)
/label=oYK98-SEAP-R
primer_bind complement(3050..3069)
/label=oYK55-SEAP-R
primer_bind complement(3051..3103)
/label=pYK62-SEAP-R
primer_bind complement(3053..3070)
/label=Oscl31-4
primer_bind complement(3053..3069)
/label=oSCL083
polyA_signal 3061..3069
/label=SV40 late polyA
misc_feature 3070..3095
/label=pA-SV40 (begin)
terminator 3210..3437
/label=bGH PA
misc_feature 3483..3911
/label=f1 origin
misc_feature 3916..4259
/label=SV40 early promoter and origin
misc_feature 4105..4182
/label=SV40 ORI
CDS 4324..5115
/codon_start=1
/label=Kan/neoR
/translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
misc_feature 5289..5419
/label=SV40 early polyadenylation signal
primer_bind complement(5455..5475)
/label=M13-rev
misc_binding complement(5481..5503)
/label=LacO
promoter complement(5508..5537)
/label=lac
misc_feature complement(5802..6475)
/label=pUC origin
CDS complement(6623..7282)
/label=AmpR
promoter complement(7522..7550)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagccctt tcgtcttcac tcgagtttac cactccctat cagtgataga
181 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
241 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
301 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
361 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
421 atcagtgata gagaaaagtg aaagtcgagc tcggtacccg gcaaggcttg accgacaatt
481 ggtatcacga ggccctttcg tcttcactcg agtttaccac tccctatcag tgatagagaa
541 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
601 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
661 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
721 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
781 agtgatagag aaaagtgaaa gtcgagctcg gtacccgcga tgtacgggcc agatatacgc
841 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
901 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
961 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
1021 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
1081 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
1141 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
1201 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
1261 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
1321 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
1381 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc tggctaacta
1441 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
1501 ctggctagca tgctgctgct gctgctgctg ctgggcctga ggctacagct ctccctgggc
1561 atcatcccag ttgaggagga gaacccggac ttctggaacc gcgaggcagc cgaggccctg
1621 ggtgccgcca agaagctgca gcctgcacag acagccgcca agaacctcat catcttcctg
1681 ggcgatggga tgggggtgtc tacggtgaca gctgccagga tcctaaaagg gcagaagaag
1741 gacaaactgg ggcctgagat acccctggcc atggaccgct tcccatatgt ggctctgtcc
1801 aagacataca atgtagacaa acatgtgcca gacagtggag ccacagccac ggcctacctg
1861 tgcggggtca agggcaactt ccagaccatt ggcttgagtg cagccgcccg ctttaaccag
1921 tgcaacacga cacgcggcaa cgaggtcatc tccgtgatga atcgggccaa gaaagcaggg
1981 aagtcagtgg gagtggtaac caccacacga gtgcagcacg cctcgccagc cggcacctac
2041 gcccacacgg tgaaccgcaa ctggtactcg gacgccgacg tgcctgcctc ggcccgccag
2101 gaggggtgcc aggacatcgc tacgcagctc atctccaaca tggacattga cgtgatccta
2161 ggtggaggcc gaaagtacat gtttcgcatg ggaaccccag accctgagta cccagatgac
2221 tacagccaag gtgggaccag gctggacggg aagaatctgg tgcaggaatg gctggcgaag
2281 cgccagggtg cccggtatgt gtggaaccgc actgagctca tgcaggcttc cctggacccg
2341 tctgtgaccc atctcatggg tctctttgag cctggagaca tgaaatacga gatccaccga
2401 gactccacac tggacccctc cctgatggag atgacagagg ctgccctgcg cctgctgagc
2461 aggaaccccc gcggcttctt cctcttcgtg gagggtggtc gcatcgacca tggtcatcat
2521 gaaagcaggg cttaccgggc actgactgag acgatcatgt tcgacgacgc cattgagagg
2581 gcgggccagc tcaccagcga ggaggacacg ctgagcctcg tcactgccga ccactcccac
2641 gtcttctcct tcggaggcta ccccctgcga gggagctcca tcttcgggct ggcccctggc
2701 aaggcccggg acaggaaggc ctacacggtc ctcctatacg gaaacggtcc aggctatgtg
2761 ctcaaggacg gcgcccggcc ggatgttacc gagagcgaga gcgggagccc cgagtatcgg
2821 cagcagtcag cagtgcccct ggacgaagag acccacgcag gcgaggacgt ggcggtgttc
2881 gcgcgcggcc cgcaggcgca cctggttcac ggcgtgcagg agcagacctt catagcgcac
2941 gtcatggcct tcgccgcctg cctggagccc tacaccgcct gcgacctggc gccccccgcc
3001 ggcaccaccg acgccgcgca cccgggttac tctagagtcg gggcggccgg ccgcttcgag
3061 cagacatgat aagatacatt gatgagtttg gacaaaagct tggtaccgag ctcggatcca
3121 ctagtccagt gtggtggaat tctgcagata tccagcacag tggcggccgc tcgagtctag
3181 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
3241 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
3301 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
3361 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
3421 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
3481 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
3541 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
3601 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
3661 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
3721 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
3781 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
3841 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
3901 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
3961 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
4021 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
4081 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
4141 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
4201 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
4261 ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc
4321 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
4381 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
4441 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
4501 caggacgagg cagcgcggct atcgtggctg gccacgacag gcgttccttg cgcagctgtg
4561 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
4621 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
4681 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
4741 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
4801 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
4861 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
4921 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
4981 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
5041 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
5101 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
5161 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
5221 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
5281 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
5341 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
5401 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
5461 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
5521 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
5581 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
5641 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
5701 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
5761 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
5821 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
5881 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
5941 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
6001 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
6061 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
6121 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
6181 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
6241 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
6301 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
6361 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
6421 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
6481 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
6541 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
6601 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
6661 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
6721 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
6781 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
6841 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
6901 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
6961 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
7021 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
7081 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
7141 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
7201 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
7261 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
7321 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
7381 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
7441 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
7501 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
7561 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc
//
pYK262载体图谱质粒图谱pdf版和pYK262载体序列质粒序列等相关资料下载
pYK262质粒载体应用举例
- 上一篇：pYK250
- 下一篇：没有了
