pYK164

LOCUS       pYK164           7326 bp DNA     circular SYN 15-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 27, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7326
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    232..819
                     /label=CMV
     primer_bind     863..882
                     /label=T7
     primer_bind     881..985
                     /label=oYK428_PB1_NES_F
     primer_bind     881..980
                     /label=oYK52-MTS-PB1-AG-F
     primer_bind     881..900
                     /label=oYK06-ANR-mEGFP-F
     primer_bind     881..900
                     /label=oYK30-NS3a_Head-F
     primer_bind     887..904
                     /label=oYK661_Nhe_F
     primer_bind     902..921
                     /label=oYK809_NLS_F
     misc_feature    905..964
                     /label=NLS2
     primer_bind     complement(943..967)
                     /label=oYK163_NLS_R
     primer_bind     complement(945..987)
                     /label=oYK164_NLS_R
     primer_bind     complement(945..972)
                     /label=oYK54-NLS-R
     primer_bind     complement(945..972)
                     /label=oYK57-NLS-R
     misc_feature    968..1270
                     /label=PB1
     primer_bind     968..985
                     /label=oYK164_PB_F
     primer_bind     968..985
                     /label=oYK509_PB1_F
     regulatory      968
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1271..1501
                     /label=Long_Linker
     primer_bind     complement(1368..1408)
                     /label=EGFP-TM-2-reverse
     misc_feature    1502..2176
                     /label=AG
     primer_bind     1950..1960
                     /label=oSCL1104
     primer_bind     complement(2162..2176)
                     /label=oYK224_AG_R
     primer_bind     complement(2164..2199)
                     /label=oYK65-AG-R
     primer_bind     2176..2194
                     /label=oYK464_NS3a2_F
     misc_feature    2177..2184
                     /label=Short_Linker
     primer_bind     2180..2194
                     /label=oYK65-NS3a-F
     primer_bind     2180..2191
                     /label=oYK29-NS3a-F
     primer_bind     complement(2191..2216)
                     /label=oYK03-02-R
     primer_bind     complement(2191..2208)
                     /label=oYK283_PB-R
     primer_bind     complement(2193..2207)
                     /label=EGFP-TM-2-reverse
     primer_bind     2197..2227
                     /label=oyk03-03-F
     misc_feature    2201..2206
                     /label=linker
     primer_bind     2204..2242
                     /label=oSCL1082
     misc_feature    2205
                     /label=kozak
     primer_bind     2206..2227
                     /label=oYK04-ns3a-F
     misc_feature    2206
                     /label=linker
     CDS             2207..2794
                     /codon_start=1
                     /label=NS3a H1
                     /translation="KKKGSVVIVGRINLSGDTAYSQQTRGLEGCQETSQTGRDKNQVEG
                     EVQVVSTATQSFLATSINGVLWTVYHGAGTRTIASPKGPVTQMYTNVDKDLVGWQAPQG
                     SRSLTPCTCGSSDLYLVTRHADVIPVRGRGDSRGSLLSPRPISYLKGSSGGPLLCPAGH
                     AVGIFRAAVSTRGVAKAVDFIPVESLETTMRSP"
     primer_bind     2207..2227
                     /label=oYK153_NS3a_F
     primer_bind     complement(2207..2226)
                     /label=oYK163_NLS_R
     primer_bind     2207..2225
                     /label=oYK25-NS3a-F
     primer_bind     2207..2224
                     /label=oYK30-NS3a_Head-F
     primer_bind     2224..2282
                     /label=oSCL1083
     primer_bind     2263..2321
                     /label=oSCL1084
     primer_bind     2300..2358
                     /label=oSCL1085
     primer_bind     2337..2395
                     /label=oSCL1086
     primer_bind     complement(2365..2384)
                     /label=oSCL1106
     primer_bind     2366..2404
                     /label=oSCL1104
     primer_bind     complement(2601..2659)
                     /label=oSCL1105
     primer_bind     complement(2641..2712)
                     /label=oSCL1087
     primer_bind     2643..2664
                     /label=oSCL1107
     primer_bind     2694..2712
                     /label=oSCL1088
     primer_bind     complement(2776..2823)
                     /label=oSCL1089
     primer_bind     complement(2779..2826)
                     /label=oYK30-NS3a_head-R
     primer_bind     complement(2782..2826)
                     /label=oYK433_NS3a_R
                     /note="GCTAGCGGGGCTTCTCATG"
     primer_bind     complement(2782..2800)
                     /label=oYK809_NS3a_R
     primer_bind     complement(2783..2826)
                     /label=oYK428_Ns3a_R
     primer_bind     complement(2795..2843)
                     /label=oYK03-03-R
     primer_bind     complement(2795..2814)
                     /label=oSCL1130
     primer_bind     complement(2803..2823)
                     /label=olyl382
     primer_bind     complement(2824..2846)
                     /label=oYK57-MCP-mCherry-R
     primer_bind     complement(2824..2843)
                     /label=oYK25-NS3a-R
     primer_bind     complement(2824..2843)
                     /label=oYK224_AG_R
     primer_bind     complement(2848..2867)
                     /label=oSCL1089
     terminator      2920..3147
                     /label=bGH PA
     misc_feature    3193..3621
                     /label=f1 origin
     misc_feature    3626..3969
                     /label=SV40 early promoter and origin
     misc_feature    3815..3892
                     /label=SV40 ORI
     CDS             4034..4825
                     /label=Kan/neoR
     misc_feature    4999..5129
                     /label=SV40 early polyadenylation signal
     primer_bind     5103..5122
                     /label=olyl382
     primer_bind     complement(5165..5185)
                     /label=M13-rev
     misc_binding    complement(5191..5213)
                     /label=LacO
     promoter        complement(5218..5247)
                     /label=lac
     misc_feature    complement(5512..6185)
                     /label=pUC origin
     CDS             complement(6333..6992)
                     /label=AmpR
     primer_bind     6649..6660
                     /label=oyk03-03-F
     primer_bind     6649..6660
                     /label=oYK04-ns3a-F
     primer_bind     6649..6660
                     /label=oYK153_NS3a_F
     promoter        complement(7232..7260)
                     /label=Amp prom
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 caccatgccc aagaagaagc gcaaggtgga agctagcgct tccccgaaga aaaagcggaa
      961 agtcatggcg tcgctcaccg tgaaggccta ccttctgggc aaggaggacg cggcgcgcga
     1021 gattcgccgc ttcagcttct gttgcagccc cgagcctgag gcggaagccg aggctgcggc
     1081 gggtccggga ccctgcgagc ggctgctgag ccgggtggcc gccctgttcc ccgcgctgcg
     1141 gcctggcggc ttccaggcgc actaccgcga tgaggacggg gacttggttg ccttttccag
     1201 tgacgaggaa ttgacaatgg ccatgtccta cgtgaaggat gacatcttcc gaatctacat
     1261 taaagagaaa accggttctg ggagtggcgg tagtggtgct ggaggcagcg caggctctgg
     1321 agcaggcggc agtgccggaa gtggcgctgg agggtctgcc ggatctggag ccggtggttc
     1381 tgccggctcc ggcgctggtg ggagcgcttc cggaagtagt cccggtaatt ccgctgacgg
     1441 cggcggagga tcgggtggta gtggtggttc aggaggagga tcgacccaag gaggatccat
     1501 ggtgagcgtg atcaagcccg agatgaagat caagctgtgc atgaggggca ccgtgaacgg
     1561 ccacaacttc gtgatcgagg gcgagggcaa gggcaacccc tacgagggca cccagatcct
     1621 ggacctgaac gtgaccgagg gcgcccccct gcccttcgcc tacgacatcc tgaccaccgt
     1681 gttccagtac ggcaacaggg ccttcaccaa gtaccccgcc gacatccagg actacttcaa
     1741 gcagaccttc cccgagggct accactggga gaggagcatg acctacgagg accagggcat
     1801 ctgcaccgcc accagcaaca tcagcatgag gggcgactgc ttcttctacg acatcaggtt
     1861 cgacggcgtg aacttccccc ccaacggccc cgtgatgcag aagaagaccc tgaagtggga
     1921 gcccagcacc gagaagatgt acgtgaggga cggcgtgctg aagggcgacg tgaacatggc
     1981 cctgctgctg gagggcggcg gccactacag gtgcgacttc aagaccacct acaaggccaa
     2041 gaaggacgtg aggctgcccg actaccactt cgtggaccac aggatcgaga tcctgaagca
     2101 cgacaaggac tacaacaagg tgaagctgta cgagaacgcc gtggccaggt acagcatgct
     2161 gcccagccag gccaagggtg gcggtgggag cggaggcggt gggtccaaga agaagggcag
     2221 cgtggtgatc gtgggcagaa tcaacctgag cggcgacacc gcctacagcc agcagaccag
     2281 aggcctggag ggctgccagg agaccagcca gaccggcaga gacaagaacc aggtggaggg
     2341 cgaggtgcag gtggtgagca ccgccaccca gagcttcctg gccaccagca tcaacggcgt
     2401 gctgtggacc gtgtaccacg gcgccggcac cagaaccatc gccagcccca agggccccgt
     2461 gacccagatg tacacgaacg tggacaagga cttagtgggc tggcaggccc cccaaggcag
     2521 cagaagcctg accccctgca cctgcggcag cagcgacctg tacctggtga ccagacacgc
     2581 cgacgtgatc cccgtgagag gaagaggcga cagcagaggc agcctgctga gccccagacc
     2641 catcagctat ctgaaaggct caagcggcgg ccccctgttg tgtcccgccg gccatgccgt
     2701 gggcatcttc agagccgccg tgagcaccag aggcgtggcc aaggccgtgg acttcatccc
     2761 cgtggagagc ctggagacca ccatgagaag ccccgctagc tagaaattct gcagatatcc
     2821 agcggatcca ctagtccagt gtggtggaat tctgcagata tccagcacag tggcggccgc
     2881 tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     2941 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     3001 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     3061 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     3121 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     3181 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     3241 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     3301 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     3361 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     3421 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     3481 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     3541 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     3601 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     3661 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     3721 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     3781 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     3841 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     3901 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     3961 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg
     4021 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     4081 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     4141 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     4201 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     4261 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     4321 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     4381 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     4441 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     4501 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     4561 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     4621 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     4681 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     4741 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     4801 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     4861 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     4921 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     4981 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     5041 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     5101 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     5161 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     5221 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     5281 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     5341 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     5401 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     5461 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     5521 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     5581 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     5641 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     5701 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     5761 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     5821 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     5881 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     5941 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     6001 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     6061 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     6121 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     6181 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     6241 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     6301 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     6361 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     6421 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     6481 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     6541 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     6601 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     6661 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     6721 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     6781 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     6841 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     6901 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     6961 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     7021 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     7081 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     7141 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     7201 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     7261 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     7321 gacgtc
//