| 质粒名称: | pHybrid-RGEPO2 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000769 |
| 产品用途: | Expressing the red potassium indicator in the cytoplasm of mammalian cells or E.coli |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pHybrid |
| 表达系统: | 哺乳动物表达、细菌表达 |
| 启动子: | rhaB, CMV, SV40 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | RGEPO2, mScarlet |
| 物种来源: | bacterium |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Sensitive red fluorescent indicators for real-time visualization of potassium ion dynamics in vivo.
Yang L., Pathiranage V., Zhou S., Sun X., Zhang H., Lai C., Gu C., Subach F.V., Drobizhev M., Walker A.R., Piatkevich K.D.
PLOS Biol. 23, e3002993 (2025)
|
买家导航
pHybrid-RGEPO2载体质粒基本信息
pHybrid-RGEPO2质粒图谱载体图谱和pHybrid-RGEPO2载体序列质粒序列多克隆位点信息
pHybrid-RGEPO2质粒载体简介
pHybrid-RGEPO2质粒序列载体序列
LOCUS pHybrid-RGEPO2 6053 bp DNA circular SYN 18-JUN-2025
DEFINITION TSN20241110-0571-00010_769_20241111-BAN3_C01_C01_i22.1 (6053 bp).
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6053)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 15, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6053
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 177..295
/label=rhaB promoter
/note="promoter of the E. coli rhaBAD operon, conferring
tight induction with L-rhamnose and repression with
D-glucose in the presence of RhaR and RhaS (Giacalone et
al., 2006)"
CDS 336..353
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
regulatory 384..393
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 390..842
/codon_start=1
/label=N-mApple
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
FQTARLKVTKGGPLPFAWDILSPQFMYGSKAYIKHPADIPDYFKLSFPEGFRWERVMSF
EDGGIIHVNQDSSLQDGVFIYKVKLLGTNFPPDGPVMQKKTMGWEAS"
CDS 843..845
/codon_start=1
/label=Linker
/translation="L"
CDS 846..1277
/codon_start=1
/label=YgaU_Hydrothermal
/translation="GIWDFVKSAGRKIGIGSEEAAPAADDLKKELDSYKLGTDDVKVEV
VGDKAVITGNVADQSILEKAIVAVGNTLGISKVESNVEVPDAKEPVFHTVKKGDTLWAI
ASKQYGNVSKYNAIFEANKPMLSHPDKIYPGQVLRIPDLG"
CDS 1278..1286
/codon_start=1
/label=Linker
/translation="SNP"
CDS 1287..1544
/codon_start=1
/label=C-Apple
/translation="VSEWMYPEDGALKSEIRKGLRLKDGGHYAAEVKTTYKAKKPVQLP
GAYIVDIELDILSHNEDYTIVEQCERAVGRHPTGGTDALYK"
terminator 1568..1654
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
polyA_signal 1678..1799
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter 1840..2050
/label=SV40 promoter
/note="SV40 early promoter"
promoter 1862..2058
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 1909..2044
/label=SV40 ori
/note="SV40 origin of replication"
regulatory 2059..2068
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 2065..2760
/codon_start=1
/product="bright monomeric red fluorescent protein evolved
from a synthetic template (Bindels et al., 2016)"
/label=mScarlet-I
/note="matures faster than mScarlet"
/translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
STGGMDELYK"
polyA_signal 2765..2812
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 2892..3347
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3679..3783
/gene="bla"
/label=AmpR promoter
CDS 3784..4644
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 4815..5403
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
enhancer 5614..5993
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter join(5994..6053,1..144)
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
ORIGIN
1 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
61 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
121 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcactag aagcttcacc
181 acaattcagc aaattgtgaa catcatcacg ttcatctttc cctggttgcc aatggcccat
241 tttcctgtca gtaacgagaa ggtcgcgaat tcaggcgctt tttagactgg tcgtaacccg
301 tttttggcta acaggaggaa ttaaccatgg ggagccacca tcaccatcac catgctagcc
361 tcgagaattc acgcgtggta ccagccacca tggtgagcaa gggcgaggag gataacatgg
421 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
481 agttcgagat cgagggcgag ggcgagggcc gcccctacga ggccttccag accgctaggc
541 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
601 tgtacggctc caaggcctac attaagcacc cagccgacat ccccgactac ttcaagctgt
661 ccttccccga gggcttcagg tgggagcgcg tgatgagctt cgaggacggc ggcattattc
721 acgttaatca ggactcctcc ctgcaggacg gcgtattcat ctacaaggtg aagctgctcg
781 gcaccaactt cccccccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggctt
841 ccctgggcat ctgggacttc gtgaagagcg ccggcaggaa gatcggcatc ggcagcgagg
901 aggccgcccc cgccgccgac gacctgaaga aggagctgga cagctacaag ctgggcaccg
961 acgacgtgaa ggtggaggtg gtgggcgaca aggccgtgat caccggcaac gtggccgacc
1021 agagcatcct ggagaaggcc atcgtggccg tgggcaacac cctgggcatc agcaaggtgg
1081 agagcaacgt ggaggtgccc gacgccaagg agcccgtgtt ccacaccgtg aagaagggcg
1141 acaccctgtg ggccatcgcc agcaagcagt acggcaacgt cagcaagtac aacgccatct
1201 tcgaggccaa caagcccatg ctgagccacc ccgacaagat ctaccccggc caggtgctga
1261 ggatccccga cctgggctct aacccggttt ccgagtggat gtaccccgag gacggcgccc
1321 tgaagagcga gatcaggaag gggctgaggc tgaaggacgg cggccactac gccgccgagg
1381 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac atcgtcgaca
1441 tcgagttgga catcttgtcc cacaacgagg actacaccat cgtggaacag tgcgaacgcg
1501 ccgtgggccg ccaccccacc ggtggcacgg acgcgctgta caagtagtct agagtcgacc
1561 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
1621 tttgtcggtg aacgctctcc tgagtaggac aaatgcggcc gcttcgagca gacatgataa
1681 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
1741 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
1801 acaacaacaa ttgggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
1861 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
1921 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
1981 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
2041 ggcctaggct tttgcaaagc caccatggtg agcaagggcg aggcagtgat caaggagttc
2101 atgcggttca aggtgcacat ggagggctcc atgaacggcc acgagttcga gatcgagggc
2161 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
2221 ggccccctgc ccttctcctg ggacatcctg tcccctcagt tcatgtacgg ctccagggcc
2281 ttcatcaagc accccgccga catccccgac tactataagc agtccttccc cgagggcttc
2341 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgccg tgaccgtgac ccaggacacc
2401 tccctggagg acggcaccct gatctacaag gtgaagctcc gcggcaccaa cttccctcct
2461 gacggccccg taatgcagaa gaagacaatg ggctgggaag cgtccaccga gcggttgtac
2521 cccgaggacg gcgtgctgaa gggcgacatt aagatggccc tgcgcctgaa ggacggcggc
2581 cgctacctgg cggacttcaa gaccacctac aaggccaaga agcccgtgca gatgcccggc
2641 gcctacaacg tcgaccgcaa gttggacatc acctcccaca acgaggacta caccgtggtg
2701 gaacagtacg aacgctccga gggccgccac tccaccggcg gcatggacga gctgtacaag
2761 tgaccggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcggatccgg
2821 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
2881 atggcgaatg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
2941 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
3001 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
3061 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
3121 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
3181 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
3241 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
3301 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttcct gatgcggtat
3361 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
3421 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
3481 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
3541 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
3601 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
3661 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
3721 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
3781 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
3841 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
3901 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
3961 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
4021 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
4081 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
4141 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
4201 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
4261 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
4321 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
4381 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
4441 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggc
4501 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
4561 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
4621 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
4681 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
4741 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
4801 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
4861 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
4921 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
4981 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
5041 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
5101 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
5161 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
5221 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
5281 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
5341 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
5401 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
5461 atggctcgac agatcttcaa tattggccat tagccatatt attcattggt tatatagcat
5521 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
5581 atattggctc atgtccaata tgaccgccat gttggcattg attattgact agttattaat
5641 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
5701 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
5761 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
5821 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
5881 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
5941 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
6001 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga ttt
//
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