| 质粒名称: | pYK582 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000827 |
| 产品用途: | Mammalian PhCMV-driven constitutive NLS-KRAB-NS3a(H1) expression vector (PhCMV-NLS-KRAB-NS3a(H1)-pA). |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | NLS-KRAB-NS3a(H1) |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Programmable solid-state condensates for spatiotemporal control of mammalian gene expression
Wang Y., Jiang J., Xiong Q., Li S., Shao J., Xie M., Zeng A.P.
Nat. Chem. Biol. 2025 1–10 (2025)
|
pYK582
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pYK582载体质粒基本信息
pYK582质粒图谱载体图谱和pYK582载体序列质粒序列多克隆位点信息
pYK582质粒载体简介
pYK582质粒序列载体序列
LOCUS pYK582 6460 bp DNA circular SYN 11-FEB-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6460)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 28, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6460
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind 881..937
/label=oYK512_KRAB_F
primer_bind 881..899
/label=oYK06-ANR-mEGFP-F
primer_bind 881..899
/label=oYK30-NS3a_Head-F
primer_bind 903..924
/label=oYW1183
CDS 903..923
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 927..947
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 948..1313
/codon_start=1
/label=KRAB(KOX1)
/translation="MDAKSLTAWSRTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLE
NYKNLVSLGYQLTKPDVILRLEKGEEPWLVEREIHQETHPDSETAFEIKSSVSSRSIFK
DKQSCDIKMEGMARNDLW"
primer_bind 948..972
/label=oYK537_KRAB_F
primer_bind 948..971
/label=oYK530_KRAB(kox)_F
primer_bind complement(1289..1313)
/label=oYW1184
primer_bind complement(1290..1333)
/label=oYK514_KRAB_R
primer_bind complement(1290..1314)
/label=oYK512_KRAB_R
primer_bind complement(1290..1313)
/label=oYK537_KRAB_R
primer_bind 1314..1328
/label=oYK65-NS3a-F
primer_bind 1314..1325
/label=oYK29-NS3a-F
misc_feature 1314..1318
/label=Short_Linker
primer_bind complement(1325..1350)
/label=oYK03-02-R
primer_bind complement(1325..1342)
/label=oYK283_PB-R
primer_bind complement(1327..1341)
/label=EGFP-TM-2-reverse
primer_bind 1331..1361
/label=oyk03-03-F
misc_feature 1335..1340
/label=linker
primer_bind 1338..1376
/label=oSCL1082
misc_feature 1339
/label=kozak
primer_bind 1340..1361
/label=oYK04-ns3a-F
misc_feature 1340
/label=linker
CDS 1341..1928
/codon_start=1
/label=NS3a H1
/translation="KKKGSVVIVGRINLSGDTAYSQQTRGLEGCQETSQTGRDKNQVEG
EVQVVSTATQSFLATSINGVLWTVYHGAGTRTIASPKGPVTQMYTNVDKDLVGWQAPQG
SRSLTPCTCGSSDLYLVTRHADVIPVRGRGDSRGSLLSPRPISYLKGSSGGPLLCPAGH
AVGIFRAAVSTRGVAKAVDFIPVESLETTMRSP"
primer_bind 1341..1361
/label=oYK153_NS3a_F
primer_bind 1341..1359
/label=oYK25-NS3a-F
primer_bind 1341..1358
/label=oYK30-NS3a_Head-F
primer_bind 1358..1416
/label=oSCL1083
primer_bind 1397..1455
/label=oSCL1084
primer_bind 1434..1492
/label=oSCL1085
primer_bind 1471..1529
/label=oSCL1086
primer_bind complement(1499..1518)
/label=oSCL1106
primer_bind 1500..1538
/label=oSCL1104
primer_bind complement(1735..1793)
/label=oSCL1105
primer_bind complement(1775..1846)
/label=oSCL1087
primer_bind 1777..1798
/label=oSCL1107
primer_bind 1828..1846
/label=oSCL1088
primer_bind complement(1910..1957)
/label=oSCL1089
primer_bind complement(1913..1960)
/label=oYK30-NS3a_head-R
primer_bind complement(1916..1960)
/label=oYK433_NS3a_R
/note="GCTAGCGGGGCTTCTCATG"
primer_bind complement(1917..1960)
/label=oYK428_Ns3a_R
primer_bind complement(1929..1977)
/label=oYK03-03-R
primer_bind complement(1929..1948)
/label=oSCL1130
primer_bind complement(1937..1957)
/label=olyl382
primer_bind complement(1958..1980)
/label=oYK57-MCP-mCherry-R
primer_bind complement(1958..1977)
/label=oYK25-NS3a-R
primer_bind complement(1982..2001)
/label=oSCL1089
primer_bind complement(2001..2017)
/label=oYK537_KRAB_R
primer_bind complement(2018..2045)
/label=oYW1184
terminator 2054..2281
/label=bGH PA
misc_feature 2327..2755
/label=f1 origin
misc_feature 2760..3103
/label=SV40 early promoter and origin
misc_feature 2949..3026
/label=SV40 ORI
CDS 3168..3959
/label=Kan/neoR
misc_feature 4133..4263
/label=SV40 early polyadenylation signal
primer_bind 4237..4256
/label=olyl382
primer_bind complement(4299..4319)
/label=M13-rev
misc_binding complement(4325..4347)
/label=LacO
promoter complement(4352..4381)
/label=lac
misc_feature complement(4646..5319)
/label=pUC origin
CDS complement(5467..6126)
/label=AmpR
primer_bind 5783..5794
/label=oyk03-03-F
primer_bind 5783..5794
/label=oYK04-ns3a-F
primer_bind 5783..5794
/label=oYK153_NS3a_F
promoter complement(6366..6394)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctaga
901 tgccaaaaaa gaagagaaag gtagatccaa aaaagaagag aaaggtaatg gatgctaagt
961 cactaactgc ctggtcccgg acactggtga ccttcaagga tgtatttgtg gacttcacca
1021 gggaggagtg gaagctgctg gacactgctc agcagatcgt gtacagaaat gtgatgctgg
1081 agaactataa gaacctggtt tccttgggtt atcagcttac taagccagat gtgatcctcc
1141 ggttggagaa gggagaagag ccctggctgg tggagagaga aattcaccaa gagacccatc
1201 ctgattcaga gactgcattt gaaatcaaat catcagtttc cagcaggagc atttttaaag
1261 ataagcaatc ctgtgacatt aaaatggaag gaatggcaag gaatgatctc tggggcggtg
1321 ggagcggagg cggtgggtcc aagaagaagg gcagcgtggt gatcgtgggc agaatcaacc
1381 tgagcggcga caccgcctac agccagcaga ccagaggcct ggagggctgc caggagacca
1441 gccagaccgg cagagacaag aaccaggtgg agggcgaggt gcaggtggtg agcaccgcca
1501 cccagagctt cctggccacc agcatcaacg gcgtgctgtg gaccgtgtac cacggcgccg
1561 gcaccagaac catcgccagc cccaagggcc ccgtgaccca gatgtacacg aacgtggaca
1621 aggacttagt gggctggcag gccccccaag gcagcagaag cctgaccccc tgcacctgcg
1681 gcagcagcga cctgtacctg gtgaccagac acgccgacgt gatccccgtg agaggaagag
1741 gcgacagcag aggcagcctg ctgagcccca gacccatcag ctatctgaaa ggctcaagcg
1801 gcggccccct gttgtgtccc gccggccatg ccgtgggcat cttcagagcc gccgtgagca
1861 ccagaggcgt ggccaaggcc gtggacttca tccccgtgga gagcctggag accaccatga
1921 gaagccccgc tagctagaaa ttctgcagat atccagcgga tccactagtc cagtgtggtg
1981 gaattctgca gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgtttaaacc
2041 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
2101 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
2161 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
2221 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
2281 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
2341 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
2401 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
2461 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
2521 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
2581 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
2641 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
2701 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
2761 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
2821 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
2881 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
2941 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
3001 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
3061 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
3121 ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
3181 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
3241 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
3301 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
3361 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
3421 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
3481 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
3541 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
3601 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
3661 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
3721 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
3781 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
3841 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
3901 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
3961 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
4021 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
4081 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
4141 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
4201 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
4261 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
4321 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
4381 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
4441 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
4501 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
4561 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
4621 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
4681 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
4741 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
4801 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
4861 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
4921 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
4981 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
5041 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
5101 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
5161 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
5221 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
5281 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
5341 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
5401 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
5461 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
5521 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
5581 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
5641 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
5701 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
5761 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
5821 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
5881 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
5941 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
6001 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
6061 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
6121 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
6181 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
6241 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
6301 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
6361 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
6421 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
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