pAAVLINKv1-pCALM1-Cre-3'-NCOA1-FLAG

LOCUS       pAAVLINKv1-pCALM1-Cre-3'-NCOA1-FLAG             6318 bp DNA     circular SYN 24-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6318)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 9, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     promoter        145..265
                     /label=pCALM1
     CDS             277..1308
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     intron          1309..1349
                     /label=Splicing Donor
     protein_bind    1356..1389
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1390..1481
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1482..3031
                     /codon_start=1
                     /gene="NCOA1"
                     /gene_synonym="bHLHe42; bHLHe74; F-SRC-1; KAT13A; 
                     RIP160;SRC1"
                     /product="nuclear receptor coactivator 1 isoform 1"
                     /EC_number="2.3.1.48"
                     /label=NCOA1
                     /note="isoform 1 is encoded by transcript variant 1;steroid
                     receptor coactivator-1; Hin-2 protein; renal carcinoma 
                     antigen NY-REN-52; class E basic helix-loop-helix protein 
                     74"
                     /db_xref="CCDS:CCDS1712.1"
                     /db_xref="GeneID:8648"
                     /db_xref="HGNC:HGNC:7668"
                     /db_xref="MIM:602691"
                     /protein_id="NP_003734.3"
                     /translation="RNDEKALLEQLVSFLSGKDETELAELDRALGIDKLVQGGGLDVLS
                     ERFPPQQATPPLIMEERPNLYSQPYSSPSPTANLPSPFQGMVRQKPSLGTMPVQVTPPR
                     GAFSPGMGMQPRQTLNRPPAAPNQLRLQLQQRLQGQQQLIHQNRQAILNQFAATAPVGI
                     NMRSGMQQQITPQPPLNAQMLAQRQRELYSQQHRQRQLIQQQRAMLMRQQSFGNNLPPS
                     SGLPVQMGNPRLPQGAPQQFPYPPNYGTNPGTPPASTSPFSQLAANPEASLANRNSMVS
                     RGMTGNIGGQFGTGINPQMQQNVFQYPGAGMVPQGEANFAPSLSPGSSMVPMPIPPPQS
                     SLLQQTPPASGYQSPDMKAWQQGAIGNNNVFSQAVQNQPTPAQPGVYNNMSITVSMAGG
                     NTNVQNMNPMMAQMQMSSLQMPGMNTVCPEQINDPALRHTGLYCNQLSSTDLLKTEADG
                     TQQVQQVQVFADVQCTVNLVGGDPYLNQPGPLGTQKPTSGPQTPQAQQKSLLQQLLTE"
     misc_feature    2347..2352
                     /label=SDS
     misc_feature    2489..2494
                     /label=SDS
     misc_feature    2492..2497
                     /label=SDS
     misc_feature    3007..3012
                     /label=SDS
     CDS             3038..3103
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3107..3538
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3251..3256
                     /label=SDS
     repeat_region   3581..3721
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    3581..3710
                     /label=AAV2 ITR
     repeat_region   3582..3722
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      3796..4251
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4533..4637
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4638..5498
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5669..6257
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
      181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
      241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
      301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
      361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
      421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
      481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
      541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
      601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
      661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
      721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
      781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
      841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
      901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
      961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
     1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
     1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
     1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
     1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
     1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
     1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
     1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
     1441 tattggtctt actgacatcc actttgcctt tctctccaca gggagaaatg atgagaaggc
     1501 tcttcttgaa cagctggtat ccttccttag tggcaaagat gaaactgagc tagctgaact
     1561 agacagagct ctgggaattg acaaacttgt tcaggggggt ggattagatg tattatcaga
     1621 gagatttcca ccacaacaag caacgccacc tttgatcatg gaagaaagac ccaaccttta
     1681 ttcccagcct tactcttctc cttctcctac tgccaatctc cctagccctt tccaaggcat
     1741 ggtcaggcaa aaaccttcac tggggacgat gcctgttcaa gtaacacctc cccgaggtgc
     1801 tttttcacct ggcatgggca tgcagcccag gcaaactcta aacagacctc cggctgcacc
     1861 taaccagctt cgacttcaac tacagcagcg attacaggga caacagcagt tgatacacca
     1921 aaatcggcaa gctatcttaa accagtttgc agcaactgct cctgttggca tcaatatgag
     1981 atcaggcatg caacagcaaa ttacacctca gccacccctg aatgctcaaa tgttggcaca
     2041 acgtcagcgg gaactgtaca gtcaacagca ccgacagagg cagctaatac agcagcaaag
     2101 agccatgctt atgaggcagc aaagctttgg gaacaacctc cctccctcat ctggactacc
     2161 agttcaaatg gggaaccccc gtcttcctca gggtgctcca cagcaattcc cctatccacc
     2221 aaactatggt acaaatccag gaaccccacc tgcttctacc agcccgtttt cacaactagc
     2281 agcaaatcct gaagcatcct tggccaaccg caacagcatg gtgagcagag gcatgacagg
     2341 aaacatagga ggacagtttg gcactggaat caatcctcag atgcagcaga atgtcttcca
     2401 gtatccagga gcaggaatgg ttccccaagg tgaggccaac tttgctccat ctctaagccc
     2461 tgggagctcc atggtgccga tgccaatccc tcctcctcag agttctctgc tccagcaaac
     2521 tccacctgcc tccgggtatc agtcaccaga catgaaggcc tggcagcaag gagcgatagg
     2581 aaacaacaat gtgttcagtc aagctgtcca gaaccagccc acgcctgcac agccaggagt
     2641 atacaacaac atgagcatca ccgtttccat ggcaggtgga aatacgaatg ttcagaacat
     2701 gaacccaatg atggcccaga tgcagatgag ctctttgcag atgccaggaa tgaacactgt
     2761 gtgccctgag cagataaatg atcccgcact gagacacaca ggcctctact gcaaccagct
     2821 ctcatccact gaccttctca aaacagaagc agatggaacc cagcaggtgc aacaggttca
     2881 ggtgtttgct gacgtccagt gtacagtgaa tctggtaggc ggggaccctt acctgaacca
     2941 gcctggtcca ctgggaactc aaaagcccac gtcaggacca cagacccccc aggcccagca
     3001 gaagagcctc cttcagcagc tactgactga atgtacagac tacaaagacc atgacggtga
     3061 ttataaagat catgacatcg actacaagga tgacgatgac aagtaactcg agataatcaa
     3121 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     3181 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     3241 ttcattttct cctccttgta taaatcctgg ttagttcttg ccacggcgga actcatcgcc
     3301 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     3361 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccatctagct ttatttgtga
     3421 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     3481 caacaattgc attcatttta tgtttcaggt tcagggggag atgtgggagg ttttttaaag
     3541 atctggagac cggtgtggca cgtgcggacc gagcggccgc aggaacccct agtgatggag
     3601 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc
     3661 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag
     3721 gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt
     3781 caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     3841 cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc
     3901 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     3961 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
     4021 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     4081 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct
     4141 attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga
     4201 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca
     4261 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     4321 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     4381 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     4441 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     4501 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     4561 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     4621 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     4681 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     4741 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     4801 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     4861 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     4921 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     4981 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     5041 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     5101 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     5161 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     5221 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     5281 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     5341 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     5401 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     5461 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     5521 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     5581 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     5641 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     5701 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     5761 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     5821 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     5881 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     5941 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     6001 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     6061 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     6121 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     6181 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     6241 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     6301 ggccttttgc tcacatgt
//