| 质粒名称: | pCMV-Flag-RIN |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000985 |
| 产品用途: | Express proteins in cells |
| 存储实验室: | Peiguo YANG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV, AmpR, SV40 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | RIN |
| 物种来源: | Drosophila |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
The divergent effects of G3BP orthologs on human stress granule assembly imply a centric role for the core protein interaction network
Yao Z., Liu Y., Chen Q., Chen X., Zhu Z., Song S., Ma X., Yang P.
Cell Rep. 43, 114617 (2024)
|
pCMV-Flag-RIN
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pCMV-Flag-RIN载体质粒基本信息
pCMV-Flag-RIN质粒图谱载体图谱和pCMV-Flag-RIN载体序列质粒序列多克隆位点信息
pCMV-Flag-RIN质粒载体简介
pCMV-Flag-RIN质粒序列载体序列
LOCUS pCMV-Flag-RIN 6075 bp DNA circular SYN 04-MAR-2022
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6075)
AUTHORS Triple Threat
TITLE Direct Submission
JOURNAL Exported Aug 2, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6075
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
regulatory 607..615
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 613..615
/codon_start=1
/product="enhanced GFP with monomerizing A206K mutation
(Zacharias et al., 2002)"
/label=initiation coden
/note="mammalian codon-optimized"
/translation="M"
CDS 616..639
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
misc_feature 646..684
/label=Primer F
CDS 664..2733
/codon_start=1
/label=Rin
/translation="MVMDATQSQQPSPQSVGREFVRQYYTLLNKAPNHLHRFYNHNSSY
IHGESKLVVGQREIHNRIQQLNFNDCHAKISQVDAQATLGNGVVVQVTGELSNDGQPMR
RFTQTFVLAAQSPKKYYVHNDIFRYQDLYIEDEQDGESRSENDEEHDVQVVGGTVDQAV
QVAGDVVAQQPQQQQAPPQPQAQVVVPQQVAPVAAGGAAPQQIFYPLPAGAAAGRPVAV
LPPAPQAAAVPLPAQFTVPPPQPIQNGVVSHEELQQQQVLVPPSVSPLVQQQQPTGTPV
LPIVPVPSAGFQQSPLVAPQLIQQQQQQQPAQLPPQQQQQQPIQQQQPLQQQQQPLAEP
EEVEVAPRQQKPPTPVEDFKTIHEQQQQEKYEAAKQQQNEPKTYANLFKSTSSSPSGFV
NAALQQQQQLQQQQQQQQSISSNTYNSSSATSGGGNGSQVSYSTTASSYNNSNGNSRLD
NGGPLPQRNNSIRNNKGDFEQRRSSNTQQFGDNQQLFLGNIPHHASEDDLREIFSRFGN
VLELRILSKAGNKVPPGMRSPLNYGFITYDDPEAVQKCLANCPLYFPENSPDGQKLNVE
EKKPRVRNDMVPRQQIGGNNLNNNMGRLGGNNGPQSRPMGNNNGSGGGMMRNNAVGNNL
RQGGGGGNGAPRLGGGGGFGQRQDNRTGGGNNQSNGPLRGAGNGQSGGGNYGRR"
misc_feature complement(2713..2751)
/label=Primer R
polyA_signal 2863..2984
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(2991..3446)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3473..3577
/gene="bla"
/label=AmpR promoter
promoter 3579..3936
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3787..3922
/label=SV40 ori
/note="SV40 origin of replication"
CDS 3971..4765
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 4997..5044
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 5373..5961
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggtcgcca ccatggatta caaggacgac gatgacaagt actcagatct cgagctcaag
661 cttatggtca tggatgcgac ccaatcgcag cagccttcgc cgcaatctgt gggacgcgag
721 tttgtccgcc agtactacac gctgttgaac aaggcgccca accacttgca tcgcttctat
781 aaccacaact cgtcgtacat ccatggagag tccaagctgg tggtcggtca gcgggagatc
841 cacaaccgaa tccagcagct gaacttcaac gattgccacg cgaagatcag ccaggttgat
901 gcccaggcca ctttgggcaa cggtgtggtg gttcaggtca ccggggagct atccaatgat
961 ggccagccga tgcggcgttt tacccagacg ttcgttctgg ccgctcagtc gccgaagaag
1021 tactacgtgc acaacgacat cttccgctat caggatctct acatcgagga cgagcaggat
1081 ggcgagtcgc gatcggagaa cgatgaggag cacgatgtgc aggtcgtcgg tggcactgtg
1141 gatcaagctg tgcaggtggc cggcgatgta gttgcccagc agccgcagca acagcaggct
1201 cctccgcagc cacaggctca ggtcgtggtg ccccagcagg tagctcctgt agcggccggt
1261 ggtgctgccc cacagcagat cttctaccca ttgccagcgg gtgctgccgc cggacgtcct
1321 gttgctgttt tgccaccagc tccgcaagcc gctgctgttc cactgcccgc gcagttcacc
1381 gttccgccgc cacagcccat ccagaacggt gtggtgtcgc acgaggagct gcagcagcag
1441 caggtgctgg tcccacccag cgtgtctccc ctggtacagc agcagcagcc gaccggcacg
1501 cctgtgttgc ccattgttcc agtgccaagc gcgggcttcc aacagtcgcc actggtggca
1561 ccacagctca tccagcagca gcagcagcag caaccggcac agctgccccc gcagcagcag
1621 cagcagcagc ctatccagca gcagcagcct ttgcaacagc agcaacaacc gctggctgag
1681 cccgaggaag tggaggtggc gccaaggcag cagaagcctc ccacgcccgt cgaggatttc
1741 aagaccattc acgagcagca gcagcaggag aagtacgagg ccgccaagca gcagcagaat
1801 gagcccaaga cgtacgcgaa tctcttcaag tccacgtcct cctcgcccag cggctttgtg
1861 aacgccgcac tgcagcagca gcaacagctc cagcagcaac agcagcagca gcagtccatc
1921 agcagcaaca cctataactc ttcctcggca acctcgggtg gcggtaatgg cagtcaggtt
1981 agctactcca ccaccgcttc gtcgtacaac aacagcaacg gcaactcgcg actggacaac
2041 ggtggcccgc tgccgcagcg aaacaactcg atccgcaaca acaagggtga ctttgagcag
2101 cgtcgctcga gcaacacgca gcagttcggt gataatcagc agctgttttt gggcaatata
2161 ccgcaccacg ccagcgagga tgatctgcgc gagatcttct cacgcttcgg aaatgtcctg
2221 gagctgcgca ttttgtccaa ggctggcaac aaggtgccac caggcatgcg cagtccgctg
2281 aactacggat tcattaccta cgacgatcct gaggcggtgc aaaagtgcct ggcgaactgc
2341 cccctatact tcccggagaa ctcacccgat ggccagaagt tgaatgtgga ggagaagaag
2401 ccgcgtgtcc gcaacgacat ggtgccgcgc cagcagatcg gtggcaacaa cctgaacaac
2461 aacatgggcc gtttgggcgg caacaatggt ccgcagtcgc gtccgatggg caacaacaat
2521 ggcagcggcg gtggcatgat gcgcaacaat gccgttggca acaatctgcg ccagggcggc
2581 ggcggaggaa acggtgctcc tcgcctggga ggcggcggtg gattcggaca gcgccaggat
2641 aaccgcaccg gtggcggcaa caaccagtct aacggaccgc tgcgcggtgc cggaaatgga
2701 cagagtggtg gtggcaacta cggacgtcgc taaggatcca ccggatctag ataactgatc
2761 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
2821 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
2881 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
2941 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaacgcgt aaattgtaag
3001 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
3061 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
3121 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
3181 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
3241 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
3301 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
3361 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
3421 gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac
3481 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
3541 ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg
3601 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
3661 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
3721 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
3781 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
3841 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
3901 tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg
3961 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
4021 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
4081 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
4141 gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
4201 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
4261 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
4321 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
4381 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
4441 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag
4501 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
4561 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
4621 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
4681 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
4741 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
4801 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
4861 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
4921 gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa
4981 ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt
5041 gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc
5101 cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt
5161 gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac
5221 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
5281 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
5341 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
5401 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
5461 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
5521 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
5581 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
5641 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
5701 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
5761 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
5821 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
5881 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
5941 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
6001 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
6061 attaccgcca tgcat
//
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