pGL3-D/EM/ER-Nicking/PegRNA-PGK-puroR-mRFP1

LOCUS       pGL3-D/EM/ER-Nicking/PegRNA-PGK-puroR-mRFP1               6506 bp DNA     circular SYN 07-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 21, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6506
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..61
                     /label=AG001
     misc_feature    41..115
                     /label=hCtRNA
     primer_bind     complement(98..118)
                     /label=AG016
     primer_bind     complement(98..116)
                     /label=AG008
     primer_bind     complement(98..115)
                     /label=AG002
     primer_bind     complement(98..115)
                     /label=AG010
     primer_bind     complement(98..115)
                     /label=AG014
     primer_bind     complement(98..115)
                     /label=OL465
     primer_bind     complement(101..116)
                     /label=OL2643
     primer_bind     complement(101..116)
                     /label=OL2648
     primer_bind     complement(101..116)
                     /label=OL2651
     primer_bind     complement(101..115)
                     /label=OL2646
     primer_bind     complement(101..115)
                     /label=OL2653
     primer_bind     complement(102..136)
                     /label=OL2641
     primer_bind     116..164
                     /label=OL2722
     primer_bind     116..152
                     /label=OL2642
     misc_feature    116..136
                     /label=DNMT1 nicking
     primer_bind     134..172
                     /label=AG017
     primer_bind     136..172
                     /label=AG022
     primer_bind     136..172
                     /label=AG011
     primer_bind     136..165
                     /label=OL2724
     primer_bind     136..165
                     /label=OL2726
     primer_bind     136..152
                     /label=OL2647
     primer_bind     136..152
                     /label=OL2652
     misc_RNA        137..212
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     137..172
                     /label=AG005
     primer_bind     137..172
                     /label=AG009
     primer_bind     137..172
                     /label=AG015
     primer_bind     141..178
                     /label=AG003
     primer_bind     complement(195..231)
                     /label=OL463
     primer_bind     complement(195..212)
                     /label=AG006
     primer_bind     complement(195..212)
                     /label=AG012
     primer_bind     complement(195..212)
                     /label=AG018
     misc_feature    213..287
                     /label=hCtRNA
     primer_bind     213..233
                     /label=AG001
     primer_bind     complement(270..316)
                     /label=AG002
     primer_bind     complement(270..288)
                     /label=AG008
     primer_bind     complement(270..287)
                     /label=AG010
     primer_bind     complement(270..287)
                     /label=AG014
     primer_bind     complement(270..287)
                     /label=AG016
     primer_bind     complement(270..287)
                     /label=OL465
     primer_bind     complement(273..307)
                     /label=OL2643
     primer_bind     complement(273..293)
                     /label=OL2648
     primer_bind     complement(273..288)
                     /label=OL2651
     primer_bind     complement(273..287)
                     /label=OL2646
     primer_bind     complement(273..287)
                     /label=OL2653
     primer_bind     complement(274..288)
                     /label=OL2641
     primer_bind     288..311
                     /label=OL2644
     misc_feature    288..307
                     /label=DNMT1-spacer
     primer_bind     292..307
                     /label=OL2645
     primer_bind     307..343
                     /label=AG005
     primer_bind     307..343
                     /label=AG015
     misc_RNA        308..383
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     308..343
                     /label=AG009
     primer_bind     308..343
                     /label=AG022
     primer_bind     308..343
                     /label=AG017
     primer_bind     308..343
                     /label=AG011
     primer_bind     308..336
                     /label=OL2724
     primer_bind     308..336
                     /label=OL2726
     primer_bind     308..335
                     /label=OL2722
     primer_bind     308..323
                     /label=OL2642
     primer_bind     308..323
                     /label=OL2647
     primer_bind     308..323
                     /label=OL2652
     primer_bind     312..349
                     /label=AG003
     primer_bind     complement(366..419)
                     /label=AG018
     primer_bind     complement(366..383)
                     /label=AG006
     primer_bind     complement(366..383)
                     /label=AG012
     primer_bind     complement(366..383)
                     /label=OL463
     misc_feature    384..394
                     /label=RT template
     primer_bind     complement(388..430)
                     /label=OL2723
     misc_feature    390
                     /label=C352A
     primer_bind     complement(392..430)
                     /label=OL2645
     primer_bind     392..407
                     /label=OL2643
     misc_feature    395..407
                     /label=PBS
     primer_bind     408..434
                     /label=AG007
     primer_bind     408..434
                     /label=AG013
     primer_bind     444..468
                     /label=AG001
     misc_feature    448..522
                     /label=hCtRNA
     primer_bind     complement(505..551)
                     /label=AG002
     primer_bind     complement(505..527)
                     /label=AG008
     primer_bind     complement(505..523)
                     /label=AG014
     primer_bind     complement(505..522)
                     /label=AG010
     primer_bind     complement(505..522)
                     /label=AG016
     primer_bind     complement(505..522)
                     /label=OL465
     primer_bind     complement(508..542)
                     /label=OL2646
     primer_bind     complement(508..522)
                     /label=OL2643
     primer_bind     complement(508..522)
                     /label=OL2648
     primer_bind     complement(508..522)
                     /label=OL2651
     primer_bind     complement(508..522)
                     /label=OL2653
     primer_bind     complement(509..522)
                     /label=OL2641
     primer_bind     522..578
                     /label=AG009
     primer_bind     523..571
                     /label=OL2724
     primer_bind     523..558
                     /label=OL2647
     misc_feature    523..542
                     /label=EMX1 nicking
     primer_bind     542..578
                     /label=AG022
     primer_bind     542..578
                     /label=AG017
     primer_bind     542..578
                     /label=AG011
     primer_bind     542..571
                     /label=OL2726
     primer_bind     542..570
                     /label=OL2722
     primer_bind     542..558
                     /label=OL2642
     primer_bind     542..558
                     /label=OL2652
     misc_RNA        543..618
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     543..578
                     /label=AG005
     primer_bind     543..578
                     /label=AG015
     primer_bind     547..584
                     /label=AG003
     primer_bind     complement(601..637)
                     /label=OL463
     primer_bind     complement(601..618)
                     /label=AG006
     primer_bind     complement(601..618)
                     /label=AG012
     primer_bind     complement(601..618)
                     /label=AG018
     misc_feature    619..693
                     /label=hCtRNA
     primer_bind     619..639
                     /label=AG001
     primer_bind     complement(676..694)
                     /label=AG008
     primer_bind     complement(676..693)
                     /label=AG002
     primer_bind     complement(676..693)
                     /label=AG010
     primer_bind     complement(676..693)
                     /label=AG014
     primer_bind     complement(676..693)
                     /label=AG016
     primer_bind     complement(676..693)
                     /label=OL465
     primer_bind     complement(679..713)
                     /label=OL2648
     primer_bind     complement(679..699)
                     /label=OL2643
     primer_bind     complement(679..694)
                     /label=OL2651
     primer_bind     complement(679..693)
                     /label=OL2646
     primer_bind     complement(679..693)
                     /label=OL2653
     primer_bind     complement(680..694)
                     /label=OL2641
     primer_bind     694..717
                     /label=OL2649
     misc_feature    694..713
                     /label=EMX1-spacer
     primer_bind     698..713
                     /label=OL2650
     primer_bind     711..749
                     /label=AG005
     primer_bind     713..749
                     /label=AG015
     misc_RNA        714..789
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     714..749
                     /label=AG009
     primer_bind     714..749
                     /label=AG022
     primer_bind     714..749
                     /label=AG017
     primer_bind     714..749
                     /label=AG011
     primer_bind     714..742
                     /label=OL2724
     primer_bind     714..742
                     /label=OL2726
     primer_bind     714..741
                     /label=OL2722
     primer_bind     714..729
                     /label=OL2642
     primer_bind     714..729
                     /label=OL2647
     primer_bind     714..729
                     /label=OL2652
     primer_bind     718..755
                     /label=AG003
     primer_bind     complement(772..790)
                     /label=AG018
     primer_bind     complement(772..789)
                     /label=AG006
     primer_bind     complement(772..789)
                     /label=AG012
     primer_bind     complement(772..789)
                     /label=OL463
     misc_feature    790..811
                     /label=RTT
     primer_bind     complement(806..847)
                     /label=OL2725
     primer_bind     complement(809..847)
                     /label=OL2650
     primer_bind     809..824
                     /label=OL2648
     primer_bind     811..851
                     /label=AG007
     misc_feature    812..824
                     /label=PBS
     primer_bind     820..851
                     /label=AG013
     primer_bind     861..885
                     /label=AG001
     misc_feature    865..939
                     /label=hCtRNA
     primer_bind     complement(922..942)
                     /label=AG008
     primer_bind     complement(922..939)
                     /label=AG002
     primer_bind     complement(922..939)
                     /label=AG010
     primer_bind     complement(922..939)
                     /label=AG014
     primer_bind     complement(922..939)
                     /label=AG016
     primer_bind     complement(922..939)
                     /label=OL465
     primer_bind     complement(925..959)
                     /label=OL2651
     primer_bind     complement(925..942)
                     /label=OL2646
     primer_bind     complement(925..940)
                     /label=OL2643
     primer_bind     complement(925..940)
                     /label=OL2648
     primer_bind     complement(925..939)
                     /label=OL2653
     primer_bind     complement(926..940)
                     /label=OL2641
     primer_bind     940..988
                     /label=OL2726
     primer_bind     940..975
                     /label=OL2652
     misc_feature    940..959
                     /label=ERCC6 nicking
     primer_bind     958..995
                     /label=AG022
     primer_bind     959..995
                     /label=AG017
     primer_bind     959..995
                     /label=AG011
     primer_bind     959..988
                     /label=OL2724
     primer_bind     959..987
                     /label=OL2722
     primer_bind     959..975
                     /label=OL2642
     primer_bind     959..975
                     /label=OL2647
     misc_RNA        960..1035
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     960..995
                     /label=AG005
     primer_bind     960..995
                     /label=AG009
     primer_bind     960..995
                     /label=AG015
     primer_bind     964..1001
                     /label=AG003
     primer_bind     complement(1018..1054)
                     /label=OL463
     primer_bind     complement(1018..1035)
                     /label=AG006
     primer_bind     complement(1018..1035)
                     /label=AG012
     primer_bind     complement(1018..1035)
                     /label=AG018
     misc_feature    1036..1110
                     /label=hCtRNA
     primer_bind     1036..1056
                     /label=AG001
     primer_bind     complement(1093..1111)
                     /label=AG010
     primer_bind     complement(1093..1111)
                     /label=OL465
     primer_bind     complement(1093..1110)
                     /label=AG002
     primer_bind     complement(1093..1110)
                     /label=AG008
     primer_bind     complement(1093..1110)
                     /label=AG014
     primer_bind     complement(1093..1110)
                     /label=AG016
     primer_bind     complement(1096..1130)
                     /label=OL2653
     primer_bind     complement(1096..1110)
                     /label=OL2643
     primer_bind     complement(1096..1110)
                     /label=OL2646
     primer_bind     complement(1096..1110)
                     /label=OL2648
     primer_bind     complement(1096..1110)
                     /label=OL2651
     primer_bind     complement(1097..1110)
                     /label=OL2641
     primer_bind     1111..1134
                     /label=OL2654
     misc_feature    1111..1130
                     /label=ERCC6-spacer
     primer_bind     1111..1125
                     /label=OL2655
     primer_bind     1128..1166
                     /label=AG017
     primer_bind     1128..1158
                     /label=OL2722
     primer_bind     1128..1146
                     /label=OL2642
     primer_bind     1129..1166
                     /label=AG015
     primer_bind     1130..1166
                     /label=AG005
     misc_RNA        1131..1206
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     1131..1166
                     /label=AG009
     primer_bind     1131..1166
                     /label=AG022
     primer_bind     1131..1166
                     /label=AG011
     primer_bind     1131..1159
                     /label=OL2724
     primer_bind     1131..1159
                     /label=OL2726
     primer_bind     1131..1146
                     /label=OL2647
     primer_bind     1131..1146
                     /label=OL2652
     primer_bind     1135..1172
                     /label=AG003
     primer_bind     complement(1189..1251)
                     /label=AG018
     primer_bind     complement(1189..1224)
                     /label=AG006
     primer_bind     complement(1189..1207)
                     /label=OL463
     primer_bind     complement(1189..1206)
                     /label=AG012
     misc_feature    1207..1221
                     /label=RTT
     primer_bind     complement(1217..1263)
                     /label=OL2727
     misc_feature    1222..1239
                     /label=PBS
     primer_bind     complement(1222..1238)
                     /label=OL2654
     primer_bind     complement(1224..1263)
                     /label=OL2655
     primer_bind     complement(1240..1263)
                     /label=OL2645
     primer_bind     complement(1240..1263)
                     /label=OL2723
     primer_bind     complement(1240..1263)
                     /label=OL2650
     primer_bind     complement(1240..1263)
                     /label=OL2725
     misc_feature    1283..1400
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1449..1959
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1543..1562)
                     /label=AG019_OL1068
     primer_bind     1733..1749
                     /label=OL446
     primer_bind     1878..1896
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             1981..2577
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1981..2000)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2477..2497
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     2548..2602
                     /label=OL901
     primer_bind     complement(2554..2577)
                     /label=OL306
     primer_bind     complement(2563..2585)
                     /label=OL384
     primer_bind     2570..2601
                     /label=OL379
     CDS             2578..2634
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     2578..2634
                     /label=OL866
     primer_bind     complement(2578..2634)
                     /label=OL867
     primer_bind     2615..2658
                     /label=OL902
     primer_bind     complement(2615..2642)
                     /label=OL966
     RBS             2617..2624
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             2635..3312
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(3290..3337)
                     /label=OL903
     primer_bind     3311..3329
                     /label=OL965
     primer_bind     3313..3335
                     /label=OL305
     polyA_signal    3459..3580
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3470..3489)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3524..3543
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3769..3792)
                     /label=OL1043
     primer_bind     3770..3792
                     /label=OL1044
     primer_bind     complement(3828..3845)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3999..4587)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4079..4098)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(4181..4200)
                     /label=OL1041
     primer_bind     complement(4314..4333)
                     /label=OL631
     primer_bind     4370..4389
                     /label=OL798
     primer_bind     complement(4611..4650)
                     /label=OL660
     primer_bind     4631..4650
                     /label=OL659
     CDS             complement(4758..5618)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5381..5400
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     5534..5553
                     /label=OL1033
     primer_bind     complement(5534..5553)
                     /label=OL1034
     promoter        complement(5619..5723)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5750..6205
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5775..5784)
                     /label=OL305
     primer_bind     complement(5837..5856)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6047..6068
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6258..6277
                     /label=AG021
     polyA_signal    6336..6384
                     /note="synthetic polyadenylation signal"
     misc_feature    6398..6489
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     6438..6457
                     /label=AG020_RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 agtgaacgga tctcgacggt atcgatcacg agactagcct agagggggta tagctcagtg
       61 gtagagcatt tgactgcaga tcaagaggtc cccggttcaa atccgggtgc cccctgccct
      121 tcagctaaaa taaagggttt tagagctaga aatagcaagt taaaataagg ctagtccgtt
      181 atcaacttga aaaagtggca ccgagtcggt gcagaggggg tatagctcag tggtagagca
      241 tttgactgca gatcaagagg tccccggttc aaatccgggt gccccctgat tcctggtgcc
      301 agaaacagtt ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg
      361 aaaaagtggc accgagtcgg tgcgtcacca ctgtttctgg caccaggttt ttttctcgac
      421 ctcgagacaa gatcacgaga ctagcctaga gggggtatag ctcagtggta gagcatttga
      481 ctgcagatca agaggtcccc ggttcaaatc cgggtgcccc ctctccccat tggcctgctt
      541 cggttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa
      601 gtggcaccga gtcggtgcag agggggtata gctcagtggt agagcatttg actgcagatc
      661 aagaggtccc cggttcaaat ccgggtgccc cctgagtccg agcagaagaa gaagttttag
      721 agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg
      781 agtcggtgcg ttgatgtgat gggagccgtt cttcttctgc tcggtttttt tctcgacctc
      841 gagacaagat cacgagacta gcctagaggg ggtatagctc agtggtagag catttgactg
      901 cagatcaaga ggtccccggt tcaaatccgg gtgccccctg tcagtgatta gtggaaatgg
      961 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
     1021 gcaccgagtc ggtgcagagg gggtatagct cagtggtaga gcatttgact gcagatcaag
     1081 aggtccccgg ttcaaatccg ggtgccccct tcaatgcttt catcaccaga gttttagagc
     1141 tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt
     1201 cggtgcactt ctatgccatc cggtgatgaa agcattgatt ttttttctcg acctcgagac
     1261 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     1321 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     1381 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggccgc
     1441 ggctcgaggg ggttggggtt gcgccttttc caaggcagcc ctgggtttgc gcagggacgc
     1501 ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg accctgggac tcgcacattc
     1561 ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg ccccccggcg
     1621 acgcttcctg ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg
     1681 tgacaaacgg aagccgcacg tctcactagt accctcgcag acggacagcg ccagggagca
     1741 atggcagcgc gccgaccgcg atgggctgtg gccaatagcg gctgctcagc agggcgcgcc
     1801 gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc
     1861 ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc
     1921 ggctccctcg ttgaccgaat caccgacctc tctccccagg gggatccacc ggagcttacc
     1981 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     2041 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     2101 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     2161 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     2221 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     2281 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     2341 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     2401 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     2461 gaaacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     2521 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgccgct
     2581 actaacttca gcctgctgaa gcaggctgga gacgtggagg agaaccctgg acctatggcg
     2641 agtagcgaag acgttatcaa agagttcatg cgtttcaaag ttcgtatgga aggttccgtt
     2701 aacggtcacg agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga aggtacccag
     2761 accgctaaac tgaaagttac caaaggtggt ccgctgccgt tcgcttggga catcctgtcc
     2821 ccgcagttcc agtacggttc caaagcttac gttaaacacc cggctgacat cccggactac
     2881 ctgaaactgt ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt cgaagacggt
     2941 ggtgttgtta ccgttaccca ggactcctcc ctgcaagacg gtgagttcat ctacaaagtt
     3001 aaactgcgtg gtaccaactt cccgtccgac ggtccggtta tgcagaaaaa aaccatgggt
     3061 tgggaagctt ccaccgaacg tatgtacccg gaagacggtg ctctgaaagg tgaaatcaaa
     3121 atgcgtctga aactgaaaga cggtggtcac tacgacgctg aagttaaaac cacctacatg
     3181 gctaaaaaac cggttcagct gccgggtgct tacaaaaccg acatcaaact ggacatcacc
     3241 tcccacaacg aagactacac catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc
     3301 accggtgctt aacgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc
     3361 catgcatcgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     3421 ctagagtcgg ggcggccggc cgcttcgagc agacatgata agatacattg atgagtttgg
     3481 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     3541 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     3601 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
     3661 caaatgtggt aaaatcgata aggatccgtc gaccgatgcc cttgagagcc ttcaacccag
     3721 tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct
     3781 ttatcatgca actcgtagga caggtgccgg cagcgctctt ccgcttcctc gctcactgac
     3841 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     3901 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     3961 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     4021 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     4081 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     4141 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     4201 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     4261 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     4321 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     4381 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     4441 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     4501 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     4561 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     4621 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     4681 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     4741 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     4801 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     4861 ggcttaccat ctggccccag tgctgcaatg ataccgcggg acccacgctc accggctcca
     4921 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     4981 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     5041 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     5101 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     5161 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     5221 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     5281 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     5341 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     5401 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     5461 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     5521 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     5581 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     5641 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     5701 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt
     5761 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     5821 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     5881 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     5941 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     6001 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     6061 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     6121 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     6181 aacaaaatat taacgcttac aatttgccat tcgccattca ggctgcgcaa ctgttgggaa
     6241 gggcgatcgg tgcgggcctc ttcgctatta cgccagccca agctaccatg ataagtaagt
     6301 aatattaagg tacgggaggt acttggagcg gccgcaataa aatatcttta ttttcattac
     6361 atctgtgtgt tggttttttg tgtgaatcga tagtactaac atacgctctc catcaaaaca
     6421 aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa
     6481 catttctcta tcgataggta ccgatt
//