pCDH-EF1-mVenus-p27K

LOCUS       pCDH-EF1-mVenus-p27K−        8438 bp DNA     circular SYN 

25-AUG-2024
DEFINITION  It encodes an mVenus fused to a mutant 

p27K-, which lacks binding 
            affinity to Cdk. This 

reporter helps to identify quiescent 
            disseminated 

tumor cells (in G0 phase of cell cycle)..
ACCESSION   .
VERSION     

.
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  

synthetic DNA construct
REFERENCE   1  (bases 1 to 8438)
  AUTHORS   

Correia AL, Guimaraes JC, Auf der Maur P, De Silva D, Trefny MP, 
  

          Okamoto R, Bruno S, Schmidt A, Mertz K, Volkmann K, 

Terracciano L, 
            Zippelius A, Vetter M, Kurzeder C, 

Weber WP, Bentires-Alj M
  TITLE     Hepatic stellate cells 

suppress NK cell-sustained breast cancer 
            dormancy.
  

JOURNAL   Nature. 2021 Jun;594(7864):566-571. doi: 10.1038/s41586

-021-03614-z.
            Epub 2021 Jun 2.
  PUBMED    34079127
REFERENCE   2  (bases 1 to 8438)
  AUTHORS   .
  TITLE     Direct 

Submission
  JOURNAL   Exported Aug 25, 2024 from SnapGene Server 

7.0.3
            https://www.biofeng.com
COMMENT     SGRef: 

number: 1; type: "Journal Article"; doi: 
            

"10.1038/s41586-021-03614-z"; journalName: "Nature"; date: 
        

    "2021-06"; volume: "594"; issue: "7864"; pages: "566-571"
FEATURES             Location/Qualifiers
     source          

1..8438
                     /mol_type="other DNA"
                  

   /organism="synthetic DNA construct"
     primer_bind     66..88
  

                   /label=M13/pUC Forward
                     

/note="In lacZ gene"
     primer_bind     80..97
                    

 /label=M13 Forward
                     /note="In lacZ gene. Also 

called M13-F20 or M13 (-21) 
                     Forward"
     

primer_bind     81..97
                     /label=M13 fwd
          

           /note="common sequencing primer, one of multiple 

similar 
                     variants"
     promoter        

112..338
                     /label=RSV promoter
                   

  /note="Rous sarcoma virus enhancer/promoter"
     LTR             

339..519
                     /label=5' LTR (truncated)
             

        /note="truncated 5' long terminal repeat (LTR) from HIV-

1"
     misc_feature    566..691
                     /label=HIV-1 

Psi
                     /note="packaging signal of human 

immunodeficiency virus 
                     type 1"
     

misc_feature    1184..1417
                     /label=RRE
          

           /note="The Rev response element (RRE) of HIV-1 allows 

for 
                     Rev-dependent mRNA export from the 

nucleus to the 
                     cytoplasm."
     CDS            

 1602..1646
                     /codon_start=1
                     

/product="antigenic peptide corresponding to amino acids 
          

           655 to 669 of the HIV envelope protein gp41 (Lutje 

Hulsik 
                     et al., 2013)"
                     

/label=gp41 peptide
                     /note="recognized by the 

2H10 single-chain llama nanobody"
                     

/translation="KNEQELLELDKWASL"
     misc_feature    1913..2029
      

               /label=cPPT/CTS
                     /note="central 

polypurine tract and central termination 
                     

sequence of HIV-1"
     promoter        2072..2283
                  

   /label=EF-1-alpha core promoter
                     /note="core 

promoter for human elongation factor 
                     EF-1-

alpha"
     LTR             2296..2564
                     

/label=5' LTR (truncated)
                     /note="truncated 5' 

long terminal repeat (LTR) from human 
                     T-cell 

leukemia virus (HTLV) type 1"
     CDS             2592..3308
       

              /codon_start=1
                     /product="Venus 

YFP with monomerizing A206K mutation (Nagai
                     et 

al., 2002; Kremers et al., 2006)"
                     

/label=mVenus
                     /note="mammalian codon-

optimized"
                     

/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
        

             

KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
        

             

GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
        

             

ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
        

             EFVTAAGITLGMDELYK"
     primer_bind     complement

(2637..2658)
                     /label=EGFP-N
                     

/note="EGFP, reverse primer"
     primer_bind     complement

(2898..2917)
                     /label=EXFP-R
                     

/note="For distinguishing EGFP variants, reverse primer"
     

primer_bind     3245..3266
                     /label=EGFP-C
       

              /note="EGFP, forward primer"
     CDS             

3951..4004
                     /codon_start=1
                     

/product="2A peptide from Thosea asigna virus capsid 
              

       protein"
                     /label=T2A
                     

/note="Eukaryotic ribosomes fail to insert a peptide bond 
         

            between the Gly and Pro residues, yielding separate 
   

                  polypeptides."
                     

/translation="EGRGSLLTCGDVEENPGP"
     CDS             4011..4610
   

                  /codon_start=1
                     /gene="pac 

from Streptomyces alboniger"
                     

/product="puromycin N-acetyltransferase"
                     

/label=PuroR
                     /note="confers resistance to 

puromycin"
                     

/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
        

             

VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
        

             

AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
        

             APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind 

    complement(4011..4030)
                     /label=Puro-R
       

              /note="Puromycin resistance gene, reverse primer. 

Also 
                     called puro-variant-R"
     primer_bind   

  4507..4527
                     /label=Puro-F
                     

/note="Puromycin resistance gene, forward primer"
     misc_feature 

   4623..5211
                     /label=WPRE
                     

/note="woodchuck hepatitis virus posttranscriptional 
              

       regulatory element"
     primer_bind     complement

(4676..4696)
                     /label=WPRE-R
                     

/note="WPRE, reverse primer"
     CDS             complement

(5094..5105)
                     /codon_start=1
                    

 /product="Factor Xa recognition and cleavage site"
                

     /label=Factor Xa site
                     

/translation="IEGR"
     LTR             5285..5518
                 

    /label=3' LTR (Delta-U3)
                     /note="self-

inactivating 3' long terminal repeat (LTR) from
                    

 HIV-1"
     polyA_signal    5590..5711
                     

/label=SV40 poly(A) signal
                     /note="SV40 

polyadenylation signal"
     primer_bind     complement

(5627..5646)
                     /label=SV40pA-R
                   

  /note="SV40 polyA, reverse primer"
     primer_bind     

5681..5700
                     /label=EBV-rev
                     

/note="SV40 polyA terminator, reverse primer"
     rep_origin      

5751..5886
                     /label=SV40 ori
                     

/note="SV40 origin of replication"
     primer_bind     complement

(5919..5935)
                     /label=M13 rev
                    

 /note="common sequencing primer, one of multiple similar 
         

            variants"
     primer_bind     complement(5919..5935)
   

                  /label=M13 Reverse
                     /note="In 

lacZ gene. Also called M13-rev"
     primer_bind     complement

(5932..5954)
                     /label=M13/pUC Reverse
            

         /note="In lacZ gene"
     protein_bind    5943..5959
       

              /label=lac operator
                     

/bound_moiety="lac repressor encoded by lacI"
                     

/note="The lac repressor binds to the lac operator to 
             

        inhibit transcription in E. coli. This inhibition can be 
  

                   relieved by adding lactose or 
                  

   isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter    

    complement(5967..5997)
                     /label=lac 

promoter
                     /note="promoter for the E. coli lac 

operon"
     protein_bind    6012..6033
                     

/label=CAP binding site
                     /bound_moiety="E. coli 

catabolite activator protein"
                     /note="CAP 

binding activates transcription in the presence 
                   

  of cAMP."
     primer_bind     complement(6150..6167)
             

        /label=L4440
                     /note="L4440 vector, 

forward primer"
     rep_origin      complement(6321..6909)
         

            /direction=LEFT
                     /label=ori
         

            /note="high-copy-number ColE1/pMB1/pBR322/pUC origin 

of 
                     replication"
     CDS             

complement(7080..7940)
                     /codon_start=1
          

           /gene="bla"
                     /product="beta-

lactamase"
                     /label=AmpR
                     

/note="confers resistance to ampicillin, carbenicillin, and
        

             related antibiotics"
                     

/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
        

             

ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
        

             

PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
        

             

EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
        

             

LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
        

             LIKHW"
     primer_bind     7703..7722
                 

    /label=Amp-R
                     /note="Ampicillin resistance 

gene, reverse primer"
     promoter        complement(7941..8045)
   

                  /gene="bla"
                     /label=AmpR 

promoter
     primer_bind     8113..8131
                     

/label=pBRforEco
                     /note="pBR322 vectors, 

upsteam of EcoRI site, forward 
                     primer"
     

primer_bind     complement(8169..8191)
                     

/label=pGEX 3'
                     /note="pGEX vectors, reverse 

primer"
     primer_bind     8291..8310
                     

/label=pRS-marker
                     /note="pRS vectors, use to 

sequence yeast selectable 
                     marker"
ORIGIN
       

 1 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg 

taacgccagg
       61 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc 

aagctgacgc gtgtagtctt
      121 atgcaatact cttgtagtct tgcaacatgg 

taacgatgag ttagcaacat gccttacaag
      181 gagagaaaaa gcaccgtgca 

tgccgattgg tggaagtaag gtggtacgat cgtgccttat
      241 taggaaggca 

acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca
      301 

gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga
  

    361 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct 

caataaagct
      421 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg 

tgactctggt aactagagat
      481 ccctcagacc cttttagtca gtgtggaaaa 

tctctagcag tggcgcccga acagggactt
      541 gaaagcgaaa gggaaaccag 

aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
      601 cacggcaaga 

ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
      661 

ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
  

    721 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa 

aacatatagt
      781 atgggcaagc agggagctag aacgattcgc agttaatcct 

ggcctgttag aaacatcaga
      841 aggctgtaga caaatactgg gacagctaca 

accatccctt cagacaggat cagaagaact
      901 tagatcatta tataatacag 

tagcaaccct ctattgtgtg catcaaagga tagagataaa
      961 agacaccaag 

gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     1021 

acagcaagcg gccactgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
  

   1081 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca 

cccaccaagg
     1141 caaagagaag agtggtgcag agagaaaaaa gagcagtggg 

aataggagct ttgttccttg
     1201 ggttcttggg agcagcagga agcactatgg 

gcgcagcgtc aatgacgctg acggtacagg
     1261 ccagacaatt attgtctggt 

atagtgcagc agcagaacaa tttgctgagg gctattgagg
     1321 cgcaacagca 

tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     1381 

tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
  

   1441 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa 

tctctggaac
     1501 agatttggaa tcacacgacc tggatggagt gggacagaga 

aattaacaat tacacaagct
     1561 taatacactc cttaattgaa gaatcgcaaa 

accagcaaga aaagaatgaa caagaattat
     1621 tggaattaga taaatgggca 

agtttgtgga attggtttaa cataacaaat tggctgtggt
     1681 atataaaatt 

attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     1741 

tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
  

   1801 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt 

ggagagagag
     1861 acagagacag atccattcga ttagtgaacg gatctcgacg 

gtatcggtta acttttaaaa
     1921 gaaaaggggg gattgggggg tacagtgcag 

gggaaagaat agtagacata atagcaacag
     1981 acatacaaac taaagaatta 

caaaaacaaa ttacaaaatt caaaatttta tcgatactag
     2041 tggatctgcg 

atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     2101 

gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta
  

   2161 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg 

gggagaaccg
     2221 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca 

acgggtttgc cgccagaaca
     2281 cagctgaagc ttcgaggggc tcgcatctct 

ccttcacgcg cccgccgccc tacctgaggc
     2341 cgccatccac gccggttgag 

tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact
     2401 gcgtccgccg 

tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc
     2461 

ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa
  

   2521 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct 

gtgaccggcg
     2581 cctactctag aatggtgagc aagggcgagg agctgttcac 

cggggtggtg cccatcctgg
     2641 tcgagctgga cggcgacgta aacggccaca 

agttcagcgt gtccggcgag ggcgagggcg
     2701 atgccaccta cggcaagctg 

accctgaagc tgatctgcac caccggcaag ctgcccgtgc
     2761 cctggcccac 

cctcgtgacc accctgggct acggcctgca gtgcttcgcc cgctaccccg
     2821 

accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
  

   2881 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg 

aagttcgagg
     2941 gcgacaccct ggtgaaccgc atcgagctga agggcatcga 

cttcaaggag gacggcaaca
     3001 tcctggggca caagctggag tacaactaca 

acagccacaa cgtctatatc accgccgaca
     3061 agcagaagaa cggcatcaag 

gccaacttca agatccgcca caacatcgag gacggcggcg
     3121 tgcagctcgc 

cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     3181 

ccgacaacca ctacctgagc taccagtcca agctgagcaa agaccccaac gagaagcgcg
  

   3241 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc 

atggacgagc
     3301 tgtacaaggg atatccatca cactggcggc cgctcgaggc 

caccatgtca aacgtgagag
     3361 tgtctaacgg gagcccgagc ctggagcgga 

tggacgccag acaagcggag caccccaagc
     3421 cttccgcctg cagaaatctc 

ttcggcccgg tcaatcatga agaactaacc cgggacttgg
     3481 agaagcactg 

ccgggatatg gaagaagcga gtcagcgcaa gtggaatgcc gacgcccaga
     3541 

atcataagcc cctggagggc agatacgagt ggcaggaggt ggagaggggc agcttgcccg
  

   3601 agttctacta caggcccccg cgccccccca agagcgcctg caaggtgctg 

gcgcaggaga
     3661 gccaggatgt cagcgggagc cgccaggcgg tgcctttaat 

tgggtctcag gcaaactctg
     3721 aggaccggca tttggtggac caaatgcctg 

actcgtcaga caatccggct gggttagcgg
     3781 agcagtgtcc agggatgagg 

aagcgacctg ctgcagaaga ttcttcttcg caaaacaaaa
     3841 gggccaacag 

aacagaagaa aatgtttcag acggttcccc gaacgctggc actgtggagc
     3901 

agacgcccaa gaagcccggc cttcgacgcc agacgggatc cgcggccgct gagggcagag
  

   3961 gaagtcttct aacatgcggt gacgtggagg agaatcccgg cccttccggg 

atgaccgagt
     4021 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc 

cagggccgta cgcaccctcg
     4081 ccgccgcgtt cgccgactac cccgccacgc 

gccacaccgt cgatccggac cgccacatcg
     4141 agcgggtcac cgagctgcaa 

gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     4201 tgtgggtcgc 

ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     4261 

cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
  

   4321 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag 

cccgcgtggt
     4381 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa 

gggtctgggc agcgccgtcg
     4441 tgctccccgg agtggaggcg gccgagcgcg 

ccggggtgcc cgccttcctg gagacctccg
     4501 cgccccgcaa cctccccttc 

tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     4561 tgcccgaagg 

accgcgcacc tggtgcatga cccgcaagcc cggtgcctga atctaggtcg
     4621 

acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
  

   4681 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct 

attgcttccc
     4741 gtatggcttt cattttctcc tccttgtata aatcctggtt 

gctgtctctt tatgaggagt
     4801 tgtggcccgt tgtcaggcaa cgtggcgtgg 

tgtgcactgt gtttgctgac gcaaccccca
     4861 ctggttgggg cattgccacc 

acctgtcagc tcctttccgg gactttcgct ttccccctcc
     4921 ctattgccac 

ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     4981 

tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
  

   5041 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc 

ccttcggccc
     5101 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc 

tctgcggcct cttccgcgtc
     5161 ttcgccttcg ccctcagacg agtcggatct 

ccctttgggc cgcctccccg cctggtacct
     5221 ttaagaccaa tgacttacaa 

ggcagctgta gatcttagcc actttttaaa agaaaagggg
     5281 ggactggaag 

ggctaattca ctcccaacga agataagatc tgctttttgc ttgtactggg
     5341 

tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
  

   5401 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg 

tctgttgtgt
     5461 gactctggta actagagatc cctcagaccc ttttagtcag 

tgtggaaaat ctctagcagt
     5521 agtagttcat gtcatcttat tattcagtat 

ttataacttg caaagaaatg aatatcagag
     5581 agtgagagga acttgtttat 

tgcagcttat aatggttaca aataaagcaa tagcatcaca
     5641 aatttcacaa 

ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     5701 

aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
  

   5761 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 

tttatttatg
     5821 cagaggccga ggccgcctcg gcctctgagc tattccagaa 

gtagtgagga ggcttttttg
     5881 gaggcctaga cttttgcaga gaccaaattc 

gtaatcatgt catagctgtt tcctgtgtga
     5941 aattgttatc cgctcacaat 

tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     6001 tggggtgcct 

aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     6061 

cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
  

   6121 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 

ctcggtcgtt
     6181 cggctgcggc gagcggtatc agctcactca aaggcggtaa 

tacggttatc cacagaatca
     6241 ggggataacg caggaaagaa catgtgagca 

aaaggccagc aaaaggccag gaaccgtaaa
     6301 aaggccgcgt tgctggcgtt 

tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     6361 cgacgctcaa 

gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     6421 

cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
  

   6481 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 

gtatctcagt
     6541 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 

aaccccccgt tcagcccgac
     6601 cgctgcgcct tatccggtaa ctatcgtctt 

gagtccaacc cggtaagaca cgacttatcg
     6661 ccactggcag cagccactgg 

taacaggatt agcagagcga ggtatgtagg cggtgctaca
     6721 gagttcttga 

agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     6781 

gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
  

   6841 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 

cagaaaaaaa
     6901 ggatctcaag aagatccttt gatcttttct acggggtctg 

acgctcagtg gaacgaaaac
     6961 tcacgttaag ggattttggt catgagatta 

tcaaaaagga tcttcaccta gatcctttta
     7021 aattaaaaat gaagttttaa 

atcaatctaa agtatatatg agtaaacttg gtctgacagt
     7081 taccaatgct 

taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     7141 

gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
  

   7201 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 

agcaataaac
     7261 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 

ctttatccgc ctccatccag
     7321 tctattaatt gttgccggga agctagagta 

agtagttcgc cagttaatag tttgcgcaac
     7381 gttgttgcca ttgctacagg 

catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     7441 agctccggtt 

cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     7501 

gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
  

   7561 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 

atgcttttct
     7621 gtgactggtg agtactcaac caagtcattc tgagaatagt 

gtatgcggcg accgagttgc
     7681 tcttgcccgg cgtcaatacg ggataatacc 

gcgccacata gcagaacttt aaaagtgctc
     7741 atcattggaa aacgttcttc 

ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     7801 agttcgatgt 

aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     7861 

gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
  

   7921 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 

ttatcagggt
     7981 tattgtctca tgagcggata catatttgaa tgtatttaga 

aaaataaaca aataggggtt
     8041 ccgcgcacat ttccccgaaa agtgccacct 

gacgtctaag aaaccattat tatcatgaca
     8101 ttaacctata aaaataggcg 

tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
     8161 ggtgaaaacc 

tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     8221 

gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
  

   8281 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg 

gtgtgaaata
     8341 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 

attcgccatt caggctgcgc
     8401 aactgttggg aagggcgatc ggtgcgggcc 

tcttcgct
//