A110-CMV-367Nterm-2A-RT-U6-pegRNA

LOCUS       A110-CMV-367Nterm-2A-RT-U6-pegRNA               7738 bp DNA     circular SYN 02-APR-2026
DEFINITION  AAV genome: expresses the N-terminal of v5 AAV-ABE from the Cbh 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7738)
  AUTHORS   Levy JM, Yeh WH, Pendse N, Davis JR, Hennessey E, Butcher R, Koblan 
            LW, Comander J, Liu Q, Liu DR
  TITLE     Cytosine and adenine base editing of the brain, liver, retina, heart
            and skeletal muscle of mice via adeno-associated viruses.
  JOURNAL   Nat Biomed Eng. 2020 Jan;4(1):97-110. doi: 
            10.1038/s41551-019-0501-5. Epub 2020 Jan 14.
  PUBMED    31937940
REFERENCE   2  (bases 1 to 7738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 4, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41551-019-0501-5"; journalName: "Nat Biomed Eng"; date: 
            "2020-01"; volume: "4"; issue: "1"; pages: "97-110"
FEATURES             Location/Qualifiers
     source          1..7738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
     primer_bind     complement(12..23)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     73..84
                     /label=L4440
                     /note="L4440 vector, forward primer"
     enhancer        153..438
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     primer_bind     complement(386..402)
                     /label=480-CMV-R
     primer_bind     401..439
                     /label=217-WKG-278-F
     promoter        439..642
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     467..492
                     /label=185-WKG-602-CMV-F
     primer_bind     562..582
                     /label=85-CMV-F
     primer_bind     593..613
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             670..723
                     /codon_start=1
                     /label=NLS
                     /translation="KRTADGSEFESPKKKRKV"
     CDS             724..1821
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N-367)
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGA"
     primer_bind     1311..1334
                     /label=67-Cas9-F1
     primer_bind     complement(1311..1334)
                     /label=116-WKG-338-R3
     primer_bind     complement(1440..1456)
                     /label=78-Cas9-R1
     primer_bind     complement(1792..1814)
                     /label=115-WKG-338-R2
     CDS             1822..2127
                     /codon_start=1
                     /label=RmaDnaBIntN
                     /translation="CLAGDTLITLADGRRVPIRELVSQQNFSVWALNPQTYRLERARVS
                     RAFCTGIKPVYRLTTRLGRSIRATANHRFLTPQGWKRVDELQPGDYLALPRRIPTAS"
     CDS             2128..2190
                     /codon_start=1
                     /label=NLS
                     /label=BPNLS
                     /note="/Transferred_From=C-terminal BPNLS"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=jonlevy"
                     /note="/modified_by=jonlevy"
                     /translation="SGGSKRTADGSEFEPKKKRKV"
     CDS             2191..2247
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2248..2301
                     /codon_start=1
                     /label=NLS
                     /translation="KRTADGSEFESPKKKRKV"
     CDS             2302..3789
                     /codon_start=1
                     /label=RT-RnaseH
                     /translation="TLNIEDEYRLHETSKEPDVSLGSTWLSDFPQAWAETGGMGLAVRQ
                     APLIIPLKATSTPVSIKQYPMSQEARLGIKPHIQRLLDQGILVPCQSPWNTPLLPVKKP
                     GTNDYRPVQDLREVNKRVEDIHPTVPNPYNLLSGLPPSHQWYTVLDLKDAFFCLRLHPT
                     SQPLFAFEWRDPEMGISGQLTWTRLPQGFKNSPTLFNEALHRDLADFRIQHPDLILLQY
                     VDDLLLAATSELDCQQGTRALLQTLGNLGYRASAKKAQICQKQVKYLGYLLKEGQRWLT
                     EARKETVMGQPTPKTPRQLREFLGKAGFCRLFIPGFAEMAAPLYPLTKPGTLFNWGPDQ
                     QKAYQEIKQALLTAPALGLPDLTKPFELFVDEKQGYAKGVLTQKLGPWRRPVAYLSKKL
                     DPVAAGWPPCLRMVAAIAVLTKDAGKLTMGQPLVILAPHAVEALVKQPPDRWLSNARMT
                     HYQALLLDTDRVQFGPVVALNPATLLPLPEEGLQHNCL"
     CDS             3802..3852
                     /codon_start=1
                     /product="Better than SV40"
                     /label=BP-SV40 NLS
                     /translation="KRTADGSEFEPKKKRKV"
     misc_feature    3861..4109
                     /label=WPRE
     primer_bind     complement(3917..3937)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     complement(3931..3956)
                     /label=20-WPRE-R
     polyA_signal    4110..4334
                     /label=BGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    complement(4348..4384)
                     /label=evopreq1-1 trimmed
     misc_feature    complement(4421..4506)
                     /label=RNA scaffold
     promoter        complement(4527..4775)
                     /label=U6
     primer_bind     complement(4585..4604)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(4755..4775)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     repeat_region   4799..4928
                     /label=AAV2 ITR
     primer_bind     complement(4845..4856)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     4906..4917
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(4947..4964)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4952..4970)
                     /label=43-WKG254-F
     primer_bind     complement(4956..4978)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5105..5560
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5119..5143)
                     /label=184-WKG-599-R
     primer_bind     complement(5162..5181)
                     /label=121-WKG-450-F1
     primer_bind     complement(5192..5211)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     complement(5194..5214)
                     /label=122-WKG-465-F
     primer_bind     5402..5423
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5404..5423
                     /label=189-WKG-612-F
     promoter        5586..5690
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5691..6551
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5909..5928)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     complement(6183..6205)
                     /label=198-WKG-623-F4
     rep_origin      6722..7310
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6798..6826)
                     /label=197-WKG-623-F3
     primer_bind     7199..7223
                     /label=225-WKG-1079-F
     primer_bind     7211..7230
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7325..7344
                     /label=422-PM1195-F1
     primer_bind     7389..7415
                     /label=79-WKG-304-F1
     primer_bind     complement(7417..7436)
                     /label=196-WKG-623-F2
     primer_bind     7464..7481
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     7530..7564
                     /label=139-WKG-445-R12
     protein_bind    7598..7619
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     7621..7643
                     /label=154-lacUV5 prom-For
     primer_bind     7628..7651
                     /label=140-WKG-448-R1
     primer_bind     7628..7651
                     /label=190-WKG-612-R
     primer_bind     7631..7653
                     /label=41-lac-R
     promoter        7634..7664
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7672..7688
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7677..7699
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7696..7712
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7701..7716
                     /label=152-M13-Rev
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggcctcta gatcagggta cccgttacat aacttacggt aaatggcccg
      181 cctggctgac cgcccaacga cccccgccca ttgacgtcaa tagtaacgcc aatagggact
      241 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
      301 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
      361 cattgtgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
      421 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
      481 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
      541 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
      601 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctctggct aactaccggt
      661 gccaccatga aacggacagc cgacggaagc gagttcgagt caccaaagaa gaagcggaaa
      721 gtcgacaaga agtacagcat cggcctggac atcggcacca actctgtggg ctgggccgtg
      781 atcaccgacg agtacaaggt gcccagcaag aaattcaagg tgctgggcaa caccgaccgg
      841 cacagcatca agaagaacct gatcggagcc ctgctgttcg acagcggcga aacagccgag
      901 gccacccggc tgaagagaac cgccagaaga agatacacca gacggaagaa ccggatctgc
      961 tatctgcaag agatcttcag caacgagatg gccaaggtgg acgacagctt cttccacaga
     1021 ctggaagagt ccttcctggt ggaagaggat aagaagcacg agcggcaccc catcttcggc
     1081 aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca cctgagaaag
     1141 aaactggtgg acagcaccga caaggccgac ctgcggctga tctatctggc cctggcccac
     1201 atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga caacagcgac
     1261 gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga ggaaaacccc
     1321 atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg ccagactgag caagagcaga
     1381 cggctggaaa atctgatcgc ccagctgccc ggcgagaaga agaatggcct gttcggaaac
     1441 ctgattgccc tgagcctggg cctgaccccc aacttcaaga gcaacttcga cctggccgag
     1501 gatgccaaac tgcagctgag caaggacacc tacgacgacg acctggacaa cctgctggcc
     1561 cagatcggcg accagtacgc cgacctgttt ctggccgcca agaacctgtc cgacgccatc
     1621 ctgctgagcg acatcctgag agtgaacacc gagatcacca aggcccccct gagcgcctct
     1681 atgatcaaga gatacgacga gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg
     1741 cagcagctgc ctgagaagta caaagagatt ttcttcgacc agagcaagaa cggctacgcc
     1801 ggctacattg acggcggagc ctgtctggct ggcgatactc tcattaccct ggccgatgga
     1861 cgacgagtgc ctattagaga actggtgtca cagcagaatt tttccgtgtg ggctctgaat
     1921 cctcagactt accgcctgga gagggctaga gtgagtagag ctttctgtac cggcatcaaa
     1981 cctgtgtacc gcctcaccac tagactgggg agatccatta gggccactgc caaccaccga
     2041 tttctcacac ctcagggctg gaaacgagtc gatgaactcc agcctggaga ttacctggct
     2101 ctgcctagga gaatccctac tgcctccagc ggaggcagta aaagaacagc agacgggagt
     2161 gagtttgagc ccaagaaaaa gagaaaggtg gctactaact tcagcctgct gaagcaggct
     2221 ggagacgtgg aggagaaccc tggacctaag aggaccgccg atggctctga gttcgagagc
     2281 cccaagaaga agcggaaggt gaccctaaat atagaagatg agtatcggct acatgagacc
     2341 tcaaaagagc cagatgtttc tctagggtcc acatggctgt ctgattttcc tcaggcctgg
     2401 gcggaaaccg ggggcatggg actggcagtt cgccaagctc ctctgatcat acctctgaaa
     2461 gcaacctcta cccccgtgtc cataaaacaa taccccatgt cacaagaagc cagactgggg
     2521 atcaagcccc acatacagag actgttggac cagggaatac tggtaccctg ccagtccccc
     2581 tggaacacgc ccctgctacc cgttaagaaa ccagggacta atgattatag gcctgtccag
     2641 gatctgagag aagtcaacaa gcgggtggaa gacatccacc ccaccgtgcc caacccttac
     2701 aacctcttga gcgggctccc accgtcccac cagtggtaca ctgtgcttga tttaaaggat
     2761 gcctttttct gcctgagact ccaccccacc agtcagcctc tcttcgcctt tgagtggaga
     2821 gatccagaga tgggaatctc aggacaattg acctggacca gactcccaca gggtttcaaa
     2881 aacagtccca ccctgtttaa tgaggcactg cacagagacc tagcagactt ccggatccag
     2941 cacccagact tgatcctgct acagtacgtg gatgacttac tgctggccgc cacttctgag
     3001 ctagactgcc aacaaggtac tcgggccctg ttacaaaccc tagggaacct cgggtatcgg
     3061 gcctcggcca agaaagccca aatttgccag aaacaggtca agtatctggg gtatcttcta
     3121 aaagagggtc agagatggct gactgaggcc agaaaagaga ctgtgatggg gcagcctact
     3181 ccgaagaccc ctcgacaact aagggagttc ctagggaagg caggcttctg tcgcctcttc
     3241 atccctgggt ttgcagaaat ggcagccccc ctgtaccctc tcaccaaacc ggggactctg
     3301 tttaattggg gcccagacca acaaaaggcc tatcaagaaa tcaagcaagc tcttctaact
     3361 gccccagccc tggggttgcc agatttgact aagccctttg aactctttgt cgacgagaag
     3421 cagggctacg ccaaaggtgt cctaacgcaa aaactgggac cttggcgtcg gccggtggcc
     3481 tacctgtcca aaaagctaga cccagtagca gctgggtggc ccccttgcct acggatggta
     3541 gcagccattg ccgtactgac aaaggatgca ggcaagctaa ccatgggaca gccactagtc
     3601 attctggccc cccatgcagt agaggcacta gtcaaacaac cccccgaccg ctggctttcc
     3661 aacgcccgga tgactcacta tcaggccttg cttttggaca cggaccgggt ccagttcgga
     3721 ccggtggtag ccctgaaccc ggctacgctg ctcccactgc ctgaggaagg gctgcaacac
     3781 aactgccttt ctggcggctc aaaaagaacc gccgacggca gcgaattcga gcccaagaag
     3841 aagaggaaag tctaagatct gataatcaac ctctggatta caaaatttgt gaaagattga
     3901 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     3961 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     4021 tagttcttgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     4081 ggctgttggg cactgacaat tccgtggtgc tgtgccttct agttgccagc catctgttgt
     4141 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     4201 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     4261 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     4321 ggtgggctct atggctcgag aaaaaaattc tagttggttt aacgcgtaac tagatagaac
     4381 cgcggataat tcgtccgagc agaagaagaa gtgctcccat ggaccgactc ggtcccactt
     4441 tttcaagttg ataacggact agccttattt aaacttgcta tgctgtttcc agcatagctc
     4501 ttaaacttct tcttctgctc ggactcggtg tttcgtcctt tccacaagat atataaagcc
     4561 aagaaatcga aatactttca agttacggta agcatatgat agtccatttt aaaacataat
     4621 tttaaaactg caaactaccc aagaaattat tactttctac gtcacgtatt ttgtactaat
     4681 atctttgtgt ttacagtcaa attaattcta attatctctc taacagcctt gtatcgtata
     4741 tgcaaatatg aaggaatcat gggaaatagg ccctcttcct gcccgacctt gcggccgcag
     4801 gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
     4861 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
     4921 gcgcgcagcc ttaattaacc taattcactg gccgtcgttt tacaacgtcg tgactgggaa
     4981 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     5041 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     5101 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     5161 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     5221 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     5281 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     5341 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     5401 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     5461 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     5521 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     5581 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     5641 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     5701 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     5761 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     5821 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     5881 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     5941 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     6001 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     6061 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     6121 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     6181 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     6241 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     6301 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     6361 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     6421 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     6481 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     6541 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     6601 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     6661 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     6721 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     6781 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     6841 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     6901 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     6961 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     7021 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     7081 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     7141 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     7201 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     7261 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     7321 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     7381 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     7441 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     7501 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     7561 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     7621 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     7681 gataacaatt tcacacagga aacagctatg accatgatta cgccagattt aattaagg
//