Srr-ires-GFP

价格：元

规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg

相关技术服务：质粒构建    基因合成    质粒大提

联系方式：I47-825O-882O

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Srr-ires-GFP载体质粒基本信息

质粒名称: Srr-ires-GFP
出品公司: Wekwikgene
目录编号: 0002358
产品用途: express serine racemase in mamalian cells
存储实验室: Neurodegeneration and Neurorepair

载体骨架基本信息
载体名称: pIRES-EGFP
表达系统: 哺乳动物表达
启动子: CMV
拷贝数: 未拷贝
载体抗性: 卡那霉素
生长温度: 37 ℃
宿主菌: DH5alpha

插入基因信息
插入基因名称: srr
物种来源: mus musculus
密码子优化: 未优化

文献引用方法
参考文献部分:
Microglial serine racemase knockout alleviates Alzheimer-like neuropathology and behavioral deficit via lactylation-mediated anti-inflammation
Zhou, J., Yang, Y., Liu, S. et al.
Commun Biol (2026)

Srr-ires-GFP质粒图谱载体图谱和Srr-ires-GFP载体序列质粒序列多克隆位点信息

Srr-ires-GFP载体图谱

Srr-ires-GFP质粒载体简介

Srr-ires-GFP质粒序列载体序列

LOCUS       Srr-ires-GFP           6338 bp DNA     circular SYN 07-APR-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Srr-ires-GFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6338)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 27, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..6338
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..213
                     /label=IRES (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.5"
                     /note="/match_length=46.0"
                     /note="/other=misc_feature"
     CDS             227..946
                     /codon_start=1
                     /label=EGFP
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             963..1046
                     /codon_start=1
                     /label=large T antigen (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.9"
                     /note="/other=CDS"
                     /translation="DHNQPYHICRGFTCFKKPPTPPPEPET"
     polyA_signal    1069..1190
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     rep_origin      complement(1224..1652)
                     /direction=LEFT
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     promoter        1679..1783
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     promoter        1785..2142
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             2177..2971
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2992..3144
                     /codon_start=1
                     /label=ble (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=40.5"
                     /note="/other=CDS"
                     /translation="MTDQATPNLPSRDFDSTAAFYERLGFGIVFRDAGWMILQRGDLML
                     EFFAHP"
     polyA_signal    3203..3250
                     /label=HSV TK poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=98.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     polyA_signal    complement(3341..3357)
                     /label=bGH poly(A) signal (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.2"
                     /note="/other=polyA_signal"
     rep_origin      3579..4167
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     enhancer        4342..4645
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
     promoter        4646..4849
                     /label=CMV promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    4872..4895
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=29.6"
                     /note="/other=misc_feature"
     CDS             4902..5918
                     /codon_start=1
                     /label=Srr
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MCAQYCISFADVEKAHINIQDSIHLTPVLTSSILNQIAGRNLFFK
                     CELFQKTGSFKIRGALNAIRGLIPDTPEEKPKAVVTHSSGNHGQALTYAAKLEGIPAYI
                     VVPQTAPNCKKLAIQAYGASIVYCDPSDESREKVTQRIMQETEGILVHPNQEPAVIAGQ
                     GTIALEVLNQVPLVDALVVPVGGGGMVAGIAITIKALKPSVKVYAAEPSNADDCYQSKL
                     KGELTPNLHPPETIADGVKSSIGLNTWPIIRDLVDDVFTVTEDEIKYATQLVWGRMKLL
                     IEPTAGVALAAVLSQHFQTVSPEVKNVCIVLSGGNVDLTSLNWVGQAERPAPYQTVSV"
     misc_feature    5949..5980
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=60.4"
                     /note="/other=misc_feature"
     misc_feature    5987..6338
                     /label=IRES (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.7"
                     /note="/match_length=62.1"
                     /note="/other=misc_feature"
ORIGIN
        1 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
       61 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtac
      121 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg
      181 gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccatgg tgagcaaggg
      241 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
      301 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
      361 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
      421 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
      481 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
      541 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
      601 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
      661 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
      721 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
      781 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
      841 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
      901 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccgcgactc
      961 tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     1021 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     1081 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     1141 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat
     1201 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     1261 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     1321 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     1381 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     1441 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     1501 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     1561 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     1621 cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg
     1681 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     1741 ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac
     1801 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     1861 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     1921 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     1981 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     2041 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     2101 aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg
     2161 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     2221 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     2281 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     2341 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     2401 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     2461 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     2521 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     2581 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     2641 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     2701 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     2761 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     2821 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     2881 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     2941 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     3001 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     3061 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     3121 atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata
     3181 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg
     3241 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
     3301 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt
     3361 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca
     3421 ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     3481 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     3541 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     3601 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     3661 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     3721 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     3781 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     3841 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     3901 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     3961 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     4021 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     4081 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     4141 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     4201 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     4261 aaccgtatta ccgccatgca ttagttatta atagtaatca attacggggt cattagttca
     4321 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     4381 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     4441 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     4501 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     4561 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     4621 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     4681 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     4741 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     4801 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa
     4861 ccgtcagatc cgctagcgct accggactca gatctgccac catgtgtgct cagtactgca
     4921 tctcctttgc tgatgttgaa aaagctcata tcaacattca agactctatc cacctcaccc
     4981 cagtgctaac aagctccatt ttgaatcaaa tagcagggcg caatcttttc ttcaaatgtg
     5041 agctcttcca gaaaactggg tcttttaaga ttcgaggtgc ccttaatgcc atcagaggct
     5101 taattcctga cacgccagaa gagaagccca aagccgtagt tactcacagc agcggaaacc
     5161 atggccaagc tctcacctat gctgctaaac tggaagggat tcctgcttac attgtggttc
     5221 cccaaacagc tcccaactgc aagaaactgg caatccaagc ctatggagca tcgatagtat
     5281 actgtgaccc aagtgacgag tccagagaaa aggtcactca aagaattatg caagaaacag
     5341 aaggcatctt ggtccatccc aaccaggagc cagcagtgat agctggacaa ggaacaattg
     5401 ccctggaagt gctgaaccag gttcccttgg tagatgcact ggtggttcca gtaggaggag
     5461 gaggaatggt tgctggaata gccattacaa ttaaggccct gaaacctagt gtgaaggtat
     5521 acgctgctga gccctcgaat gcagatgact gctaccagtc taaactgaaa ggagaactga
     5581 cccccaatct tcatcctcca gaaaccatag cagatggtgt caaatccagc attggcttga
     5641 atacctggcc tattataaga gaccttgtgg atgatgtctt cactgtcacc gaagatgaaa
     5701 tcaagtatgc aacccagctg gtgtggggga gaatgaaact gctcattgag ccgactgctg
     5761 gcgtggcact ggctgctgtg ctgtctcagc atttccaaac agtctctcca gaagtaaaga
     5821 acgtctgcat tgtactcagt ggggggaatg tagacctaac ctccctgaac tgggtggggc
     5881 aggctgaacg gccagctcct taccagacgg tttctgttta cccatacgac gtcccagact
     5941 acgcttaact gcagtcgacg gtaccgcggg cccgggatcc gcccctctcc ctccccccta
     6001 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt
     6061 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
     6121 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
     6181 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
     6241 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
     6301 aagatacacc tgcaaaggcg gcacaacccc agtgccac
//

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