| 质粒名称: | pDAC565 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 195242 |
| 存储实验室: | Jennifer Doudna |
|
载体骨架基本信息 |
|
| 载体名称: | pUC19 |
| 载体出品公司: | Clontech |
| 完整质粒大小: | 11297bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | LAG-NLS-Csm1; FLAG-NLS-Csm2, FLAG-NLS-Csm3(RNase mut)-GFP; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA |
| 物种来源: | Streptococcus thermophilus |
|
文献引用方法 |
|
| 材料和方法部分: | pDAC565 was a gift from Jennifer Doudna (Addgene plasmid # 195242 ; http://n2t.net/addgene:195242 ; RRID:Addgene_195242) |
| 参考文献部分: | Precise transcript targeting by CRISPR-Csm complexes. Colognori D, Trinidad M, Doudna JA. Nat Biotechnol. 2023 Jan 23. doi: 10.1038/s41587-022-01649-9. 10.1038/s41587-022-01649-9 PubMed 36690762 |
买家导航
pDAC565载体质粒基本信息
pDAC565质粒图谱载体图谱和pDAC565载体序列质粒序列多克隆位点信息
pDAC565质粒载体简介
pDAC565质粒序列载体序列
LOCUS pDAC565 11297 bp DNA circular SYN 14-APR-2025
DEFINITION Mammalian expression of Csm-GFP complex (RNase mut) from separate
promoters (Used for RNA imaging).
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11297)
AUTHORS Colognori D, Trinidad M, Doudna JA
TITLE Precise transcript targeting by CRISPR-Csm complexes.
JOURNAL Nat Biotechnol. 2023 Jan 23. doi: 10.1038/s41587-022-01649-9.
PUBMED 36690762
REFERENCE 2 (bases 1 to 11297)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat
Biotechnol. 2023 Jan 23. doi: 10.1038/s41587-022-01649-9."
COMMENT Sequence Label: pDAC565
FEATURES Location/Qualifiers
source 1..11297
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 49..252
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 292..309
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
CDS 316..339
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS 346..366
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 2650..2697
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
promoter 2728..2931
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
regulatory 2962..2971
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 2971..2994
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS 3001..3021
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 3409..3456
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
promoter 3487..3690
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
regulatory 3721..3730
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 3730..3753
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS 3760..3780
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS join(4465..4467,4468..4470,4471..5184)
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS join(4465..4467,4468..4470,4471..5181)
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(4510..4531)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(4771..4790)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 5118..5139
/label=EGFP-C
/note="EGFP, forward primer"
polyA_signal 5185..5232
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
promoter 5263..5466
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
regulatory 5497..5506
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 5506..5529
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS 5536..5556
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 6463..6510
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
promoter 6541..6744
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
regulatory 6775..6784
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 6784..6807
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS 6814..6834
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 7915..7962
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
promoter 7993..8196
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
regulatory 8227..8236
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 8236..8259
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS 8266..8286
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 9025..9072
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
promoter 9103..9351
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA
(Domitrovich & Kunkel, 2003)"
primer_bind 9103..9123
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 9274..9293
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
primer_bind complement(9505..9523)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 9591..9695
/gene="bla"
/label=AmpR promoter
CDS join(9696..9764,9765..10556)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(9914..9933)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin join(10727..11297,1..18)
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 11216..11235
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tcttaagcgt gatgcggttt
61 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
121 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
181 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
241 ataagcagag ctcgtttagt gaaccgtcag atctctagag ccgccaccat gcaccatcac
301 catcaccatt ccggcgatta caaagacgat gacgataaga tggcgcctaa gaagaaacgc
361 aaagtgcggg gcatgaagaa agaaaagatt gatctgtttt acggagccct gctgcacgac
421 atcggaaagg tcatccagcg agcaaccgga gagcggaaga aacacgcact tgtgggcgcc
481 gactggttcg acgagatcgc cgacaaccaa gtcatctcgg atcagatccg gtaccatatg
541 gccaactacc agtctgataa gctcggcaac gatcacctgg cttacatcac ctacattgcc
601 gacaacatcg cctccggtgt cgaccgccgg caatccaacg aagagtcaga cgaagatacc
661 tccgcaaaga tctgggacac ctacacgaac caggccgaca tctttaacgt gttcggagcg
721 cagaccgata agcggtactt caagcctacc gtgctgaatc tcaagtcgaa gcccaacttc
781 gcgtccgcca cttacgaacc ctttagcaag ggcgattacg ctgccatcgc cacccggatt
841 aagaacgaac tggccgagtt cgagttcaac caagtccaga ttgactccct gctcaacctt
901 ttcgaggcta ctctctcctt cgtgccgtca agcaccaaca ctaaggaaat cgccgacatc
961 tccctggccg accattcccg cttgactgct gccttcgctc tggcgatcta cgactacctg
1021 gaggacaagg gtcggcacaa ctacaaagag gacctgttca ccaaagtgtc agcgttctat
1081 gaagaagaag ccttcctgct ggcctccttc gacctgtcgg gaatccagga ctttatctac
1141 aacattaaca tcgcaactaa cggcgcggcg aagcagctga aggcccggag cctctacctg
1201 gactttatgt ccgagtacat cgccgatagc ctgctggaca agctgggact gaacagggct
1261 aacatgcttt acgtcggcgg cggacacgcc tacttcgtcc tggccaacac cgaaaagact
1321 gtggaaaccc tggtgcagtt tgagaaggat ttcaaccagt tcctgttggc aaacttccag
1381 acccgcctct atgtggcctt tggctggggt tccttcgcgg ccaaggacat catgtccgag
1441 ctgaatagcc ccgagtccta ccgccaagtg taccaaaagg cttcgcgcat gatctccaaa
1501 aagaaaatct ccagatacga ctaccagaca ctgatgctcc tgaatcgcgg tggaaagtcc
1561 tcagagagag agtgcgagat ttgccactcc gtggagaacc tggtgtccta ccacgaccag
1621 aaagtctgtg acatttgccg gggactgtac cagttctcga aagaaattgc ccatgaccac
1681 ttcatcatta ccgaaaatga ggggctgccg attggaccaa acgcgtgctt aaagggcgtg
1741 gcattcgaaa agctgtccca agaagcgttc agccgggtct acgtgaagaa tgactataag
1801 gccggtaccg tgaaggctac gcatgtgttc gtgggggatt accagtgcga cgagatctac
1861 aactacgccg ccctgagcaa gaacgagaac ggcctaggca tcaagagact ggccgtggtc
1921 cggctcgacg tggatgactt gggcgccgcc ttcatggccg gtttcagcca gcagggaaac
1981 ggacaatact ccactctgtc aagatcggcc acattctccc ggagcatgtc gctgttcttc
2041 aaagtgtaca ttaaccagtt cgcctccgac aagaagctga gcattatcta cgcgggcggc
2101 gatgacgtgt tcgccattgg atcgtggcag gatatcatcg cgttcactgt ggaacttcgc
2161 gaaaacttca tcaagtggac caacgggaag ctcaccctct ccgcggggat agggttgttc
2221 gccgacaaga ctcctattag cctgatggct caccagaccg gggaactgga agaggccgcc
2281 aagggcaacg aaaaggactc catctcgctg ttctcaagcg actacacttt caagtttgat
2341 aggttcatca ctaacgtgta cgacgacaaa ctggaacaga ttagatactt cttcaaccat
2401 caagacgaga ggggaaagaa cttcatctat aagcttattg agcttttgag gaaccacgac
2461 cgcatgaata tggcacgcct cgcctattac ctcactcgcc tggaagaact gacccgggag
2521 actgacaggg acaagttcaa gaccttcaag aacctgttct actcctggta caccaacaag
2581 aacgataagg accggaagga agccgagctc gcgctcctgc tgtacatcta cgaaatcaga
2641 aaggattaac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcgca
2701 cacattagct agccgtcagc acacattgtg atgcggtttt ggcagtacat caatgggcgt
2761 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
2821 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
2881 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
2941 aaccgtcaga tctctagagc cgccaccatg gattacaaag acgatgacga taagatggcg
3001 cctaagaaga aacgcaaagt gcggggcatg accatcctga ccgacgagaa ctacgtggac
3061 atcgccgaga aagccatcct gaagctggaa agaaacacca gaaatagaaa gaaccctgat
3121 gccttcttcc tgaccacatc taagctgcgg aacctgctga gcctgacaag caccctgttc
3181 gacgagagca aggtgaagga atacgacgcc ctgctggaca gaatcgctta tctgagagtg
3241 cagttcgtgt accaggccgg cagagagatc gccgtgaaag atctgatcga gaaggcccag
3301 atcctggaag ctctgaaaga gatcaaggac cgggaaaccc tgcagagatt ctgcagatac
3361 atggaagccc tggtggccta cttcaagttc tacggcggca aggactgacg gcaataaaaa
3421 gacagaataa aacgcacggt gttgggtcgt ttgttcagtt cttttgcctt actttcaatg
3481 catgcggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
3541 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
3601 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
3661 gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat ctctagagcc
3721 gccaccatgg attacaaaga cgatgacgat aagatggcgc ctaagaagaa acgcaaagtg
3781 cggggcatga ccttcgccaa gatcaaattc agcgcccaga tccggctgga aaccggcctg
3841 cacatcggag gatctgatgc ctttgccgct atcggcgcca tcgccagccc tgtgatcaag
3901 gaccccatca ccaacctgcc tatcatcccc ggctctagcc tgaagggcaa gatgagaaca
3961 ctgctggcca aggtgtacaa cgaaaaggtg gccgagaagc ctagcgacga cagcgacatc
4021 ctgagcagac tgttcggaaa tagcaaggat aagcggttca agatgggcag actgatcttc
4081 cgggacgcct tcctgagcaa cgccgacgag ctggattctc tgggcgtgcg gagctacacc
4141 gaggtgaagt tcgagaacac catcgataga atcaccgccg aggccaatcc tagacagatc
4201 gagagagcca ttcggaactc aacattcgac ttcgagctga tctacgagat cactgatgag
4261 aatgagaacc aggtcgagga agatttcaag gtgatcagag acggcctgaa gctgctggaa
4321 ctggactacc tgggcggaag cggctccaga ggctacggca aagtggcttt tgagaacctg
4381 aaagccacca cagtgttcgg caactacgac gtgaaaaccc tgaacgagct gctgaccgcc
4441 gaagtgggcg gaggtgctgc cgcaatggtg agcaagggcg aggagctgtt caccggggtg
4501 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
4561 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
4621 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
4681 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
4741 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
4801 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
4861 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
4921 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
4981 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
5041 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
5101 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
5161 ggcatggacg agctgtacaa gtgacggcaa taaaaagaca gaataaaacg cacggtgttg
5221 ggtcgtttgt tctggattgc gagaatggac tagtagcaaa ctgtgatgcg gttttggcag
5281 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
5341 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
5401 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
5461 agagctcgtt tagtgaaccg tcagatctct agagccgcca ccatggatta caaagacgat
5521 gacgataaga tggcgcctaa gaagaaacgc aaagtgcggg gcatgactta caagctctac
5581 attatgacct ttcaaaacgc ccacttcggt tccggcactc tggactcatc gaagctgacc
5641 ttctccgcgg atagaatctt ctcggcactc gtgctcgagg ctctgaagat gggaaagctc
5701 gacgccttct tggccgaggc caaccaggat aagttcactc tgaccgacgc gttcccattc
5761 caattcggtc ctttcctgcc gaaaccgatt ggttacccca agcacgacca gatcgaccag
5821 tctgtggacg tgaaggaagt ccgccgccaa gcgaagctgt ccaaaaagct ccagttcctg
5881 gctctggaaa acgtcgacga ctacctgaac ggagagctgt ttgagaatga ggaacacgcc
5941 gtgatcgaca cagtgaccaa gaaccagccc cataaagatg ataatctgta ccaagtggcc
6001 accactcggt tctcgaacga cacctccctt tacgtgatcg ccaacgaatc cgatctgctg
6061 aacgaactga tgagcagcct tcagtactcc gggctgggcg gcaaaaggtc ctcaggattc
6121 ggcagatttg agctggacat ccagaacatt cccttggaac tgtccgaccg gctgacgaag
6181 aaccacagcg acaaggtcat gtcacttacc accgccctcc cggtggacgc tgatctcgag
6241 gaagcgatgg aagatggcca ttacctgttg accaagtcgt ccggattcgc attctcccac
6301 gccaccaacg aaaactatcg gaagcaggac ctgtacaagt tcgcctccgg gagcaccttc
6361 agcaagactt tcgagggaca gatcgtggac gtgcgccctc tcgatttccc tcacgccgtg
6421 ctgaactacg ccaagccgct gttctttaag ctcgaagtct aacggcaata aaaagacaga
6481 ataaaacgca cggtgttggg tcgtttgttc gcacattcaa aaacaggcaa ttggacaagc
6541 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
6601 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
6661 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
6721 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatctctag agccgccacc
6781 atggattaca aagacgatga cgataagatg gcgcctaaga agaaacgcaa agtgcggggc
6841 atgaaaaatg actaccggac cttcaagctg agcctgctga ccctggctcc tatccacatc
6901 ggcaacggcg agaagtacac cagcagagaa ttcatctacg agaacaagaa gttctacttc
6961 cccgacatgg gcaagttcta caacaagatg gtggaaaaga gactggccga gaagttcgag
7021 gccttcctga tccagaccag acccaacgcc agaaacaacc ggctgatttc ttttctgaac
7081 gacaacagaa tcgccgaaag atcttttggc ggctacagca tcagtgaaac cggcctggaa
7141 tctgataaga accctaacag cgccggagct atcaacgagg tgaacaaatt catccgggac
7201 gccttcggaa atccttacat cccaggcagc agcctgaagg gcgccatccg caccatcctg
7261 atgaacacca cacctaagtg gaacaacgag aacgccgtga acgacttcgg cagattccca
7321 aaggaaaaca agaacctgat cccttgggga cctaagaaag gcaaggaata cgacgacctg
7381 ttcaacgcca tcagagtgtc cgacagcaag cccttcgaca acaaaagcct gatcctcgtg
7441 cagaagtggg actacagcgc caaaaccaac aaggccaagc ctctgcctct gtacagagag
7501 tctatcagcc ctctgaccaa gatcgagttc gagataacaa caaccactga tgaggccggc
7561 agactgatcg aggaactggg aaagcgggcc caggcctttt ataaggacta caaggccttt
7621 ttcctgtctg aattccctga tgataagatc caggctaatc tgcaataccc catctacctg
7681 ggcgccggca gcggcgcttg gacaaagacc ctgtttaagc aggccgacgg catcctgcag
7741 cggagatact ccagaatgaa aaccaagatg gtcaagaagg gcgtgctgaa gctgacaaag
7801 gcccctctga aaacagtgaa gatccccagc ggcaaccaca gcctggtgaa gaatcacgag
7861 agcttctacg agatgggcaa agccaacttc atgatcaagg aaatcgacaa gtgacggcaa
7921 taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcatgtgtgc cggccggaga
7981 ataaaagtct aagtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
8041 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
8101 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
8161 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatctct
8221 agagccgcca ccatggatta caaagacgat gacgataaga tggcgcctaa gaagaaacgc
8281 aaagtgcggg gcatgaaaaa gctcgtgttc acctttaagc ggatcgacca ccctgctcag
8341 gacctggccg tgaaattcca cggcttcctg atggaacagc tggatagcga ctacgtggac
8401 tacctgcacc agcagcagac caacccctac gccacaaagg tgatccaggg caaagagaac
8461 acccagtggg tcgtgcatct gctgacagac gacatcgagg acaaggtgtt catgaccctg
8521 ctgcagatca aggaagtgtc cctgaacgac ctgcctaagt tgtctgtgga aaaggtggaa
8581 atccaggagc tgggcgctga taagctgctc gagatcttca acagcgagga aaaccagacc
8641 tacttcagca tcatcttcga gacacctaca ggctttaaaa gccagggcag ctacgtgatc
8701 ttccccagca tgcggctgat ctttcagagc ctgatgcaga agtacggcag actggtggaa
8761 aaccagcctg agatcgagga agataccctg gactacctga gcgagcacag caccatcacc
8821 aattacagac tggaaacaag ctacttcaga gtgcatagac agagaatccc cgccttccgg
8881 ggcaagctga ccttcaaggt gcagggagcc cagacactga aggcctacgt gaagatgctg
8941 ctgaccttcg gcgagtacag cggcctgggc atgaaaacca gcctgggaat gggcggcatc
9001 aagctggaag aaagaaagga ctgacggcaa taaaaagaca gaataaaacg cacggtgttg
9061 ggtcgtttgt tcgtgcgata gagggatcca gtgcaccggt tggagggcct atttcccatg
9121 attccttcat atttgcatat acgatacaag gctgttagag agataattag aattaatttg
9181 actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg
9241 tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga
9301 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac cgatataaac
9361 ctaattacct cgagagggga cggaaacccg tcttcgatga agcgattcag aagacttgat
9421 ataaacctaa ttacctcgag aggggacttt tttacatgtg tcagaggttt tcaccgtcat
9481 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
9541 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg ctcggaaccc
9601 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
9661 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
9721 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
9781 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
9841 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
9901 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
9961 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
10021 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
10081 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
10141 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
10201 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
10261 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
10321 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
10381 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
10441 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
10501 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
10561 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
10621 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
10681 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
10741 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
10801 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
10861 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
10921 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
10981 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
11041 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
11101 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
11161 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
11221 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
11281 tgtgatgctc gtcaggg
//
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