| 质粒名称: | px330-U6-CasMINI gRNA(Bbs1)-CAG-CasMINI-P2A-mCherry |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002336 |
| 产品用途: | A human codon-optimized CasMINI and chimeric guide RNA expression plasmid. |
| 存储实验室: | Guanghai Xiang lab |
|
载体骨架基本信息 |
|
| 载体名称: | px330 |
| 表达系统: | 哺乳动物表达 |
| 启动子: |
CAG
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Dh5a |
|
插入基因信息 |
|
| 插入基因名称: | Nuclease, mCherry |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Comprehensive assessment of activity, specificity, and safety of hypercompact TnpB systems for gene editing.
Xin C., Xiang G., Cao S., Wang Y., Yuan S., Liu X., Huo Y., Sun J., Wan X., Liu D., Hong J., Hu J., Wang H.
Genome Biol. 2026 (2026)
|
px330-U6-CasMINI gRNA(Bbs1)-CAG-CasMINI-P2A-mCherry
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
px330-U6-CasMINI gRNA(Bbs1)-CAG-CasMINI-P2A-mCherry载体质粒基本信息
px330-U6-CasMINI gRNA(Bbs1)-CAG-CasMINI-P2A-mCherry质粒图谱载体图谱和px330-U6-CasMINI gRNA(Bbs1)-CAG-CasMINI-P2A-mCherry载体序列质粒序列多克隆位点信息
px330-U6-CasMINI gRNA(Bbs1)-CAG-CasMINI-P2A-mCherry质粒载体简介
px330-U6-CasMINI gRNA(Bbs1)-CAG-CasMINI-P2A-mCherry质粒序列载体序列
LOCUS px330-U6-CasMINI gRNA(Bbs1)-CAG-CasMINI-P2A-mCherry 6703 bp DNA circular SYN 20-APR-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6703)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Aug 26, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6703
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 49..68
/label=chicken beta-actin promoter
/note="contains a 21-bp deletion relative to the standard
chicken beta-actin promoter"
intron 207..434
/label=hybrid intron
/note="hybrid between chicken beta-actin (CBA) and minute
virus of mice (MMV) introns (Gray et al., 2011)"
regulatory 446..455
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 452..2089
/codon_start=1
/label=Cas9MINI
/translation="MAPKKKRKVGIHGVPAAMAKNTITKTLKLRIVRPYNSAEVEKIVA
DEKNNREKIALEKNKDKVKEACSKHLKVAAYCTTQVERNACLFCKARKLDDKFYQKLRG
QFPDAVFWQEISEIFRQLQKQAAEIYNQSLIELYYEIFIKGKGIANASSVEHYLSRVCY
RRAAALFKNAAIASGLRSKIKSNFRLKELKNMKSGLPTTKSDNFPIPLVKQKGGQYTGF
EISNHNSDFIIKIPFGRWQVKKEIDKYRPWEKFDFEQVQKSPKPISLLLSTQRRKRNKG
WSKDEGTEAEIKKVMNGDYQTSYIEVKRGSKIGEKSAWMLNLSIDVPKIDKGVDPSIIG
GIDVGVKSPLVCAINNAFSRYSISDNDLFHFNKKMFARRRILLKKNRHKRAGHGAKNKL
KPITILTEKSERFRKKLIERWACEIADFFIKNKVGTVQMENLESMKRKEDSYFNIRLRG
FWPYAEMQNKIEFKLKQYGIEIRKVAPNNTSKTCSKCGHLNNYFNFEYRKKNKFPHFKC
EKCNFKENADYNAALNISNPKLKSTKEEP"
CDS 458..478
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 2090..2137
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
CDS 2147..2203
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 2204..2914
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
DDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
misc_feature 2220..2225
/label=SDS
polyA_signal 2945..3152
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
repeat_region 3161..3301
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3161..3290
/label=AAV2 ITR
rep_origin 3376..3831
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4113..4217
/gene="bla"
/label=AmpR promoter
CDS 4218..5078
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5249..5837
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 5899..6147
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA
(Domitrovich & Kunkel, 2003)"
enhancer 6346..6631
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer;
contains an 18-bp deletion relative to the standard CMV
enhancer"
promoter join(6633..6703,1..206)
/label=chicken beta-actin promoter
/note="contains a 21-bp deletion relative to the standard
chicken beta-actin promoter"
ORIGIN
1 tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggg cgcgcgccag
61 gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca
121 atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct
181 ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc
241 gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg
301 tgagcgggcg ggacggccct tctcctccgg gctgtaatta gctgagcaag aggtaagggt
361 ttaagggatg gttggttggt ggggtattaa tgtttaatta cctggagcac ctgcctgaaa
421 tcactttttt tcaggttgga ccggtgccac catggcccca aagaagaagc ggaaggtcgg
481 tatccacgga gtcccagcag ccatggccaa gaacacaatt acaaagacac tgaagctgag
541 gatcgtgaga ccatacaaca gcgctgaggt cgagaagatt gtggctgatg aaaagaacaa
601 cagggaaaag atcgccctcg agaagaacaa ggataaggtg aaggaggcct gctctaagca
661 cctgaaagtg gccgcctact gcaccacaca ggtggagagg aacgcctgtc tgttttgtaa
721 agctcggaag ctggatgata agttttacca gaagctgcgg ggccagttcc ccgatgccgt
781 cttttggcag gagattagcg agatcttcag acagctgcag aagcaggccg ccgagatcta
841 caaccagagc ctgatcgagc tctactacga gatcttcatc aagggcaagg gcattgccaa
901 cgcctcctcc gtggagcact acctgagcag agtgtgctac agaagagccg ccgctctctt
961 taagaacgcc gctatcgctt ccgggctgag gagcaagatt aagagtaact tccggctcaa
1021 ggagctgaag aacatgaaga gcggcctgcc cactacaaag agcgacaact tcccaattcc
1081 actggtgaag cagaaggggg gccagtacac agggttcgag atttccaacc acaacagcga
1141 ctttattatt aagatcccct ttggcaggtg gcaggtcaag aaggagattg acaagtacag
1201 gccctgggag aagtttgatt tcgagcaggt gcagaagagc cccaagccta tttccctgct
1261 gctgtccaca cagcggcgga agaggaacaa ggggtggtct aaggatgagg ggaccgaggc
1321 cgagattaag aaagtgatga acggcgacta ccagacaagc tacatcgagg tcaagcgggg
1381 cagtaagatt ggcgagaaga gcgcctggat gctgaacctg agcattgacg tgccaaagat
1441 tgataagggc gtggatccca gcatcatcgg agggatcgat gtgggggtca agagccccct
1501 cgtgtgcgcc atcaacaacg ccttcagcag gtacagcatc tccgataacg acctgttcca
1561 ctttaacaag aagatgttcg cccggcggag gattttgctc aagaagaacc ggcacaagcg
1621 ggccggacac ggggccaaga acaagctcaa gcccatcact atcctgaccg agaagagcga
1681 gaggttcagg aagaagctca tcgagagatg ggcctgcgag atcgccgatt tctttattaa
1741 gaacaaggtc ggaacagtgc agatggagaa cctcgagagc atgaagagga aggaggattc
1801 ctacttcaac attcggctga gggggttctg gccctacgct gagatgcaga acaagattga
1861 gtttaagctg aagcagtacg ggattgagat ccggaaggtg gcccccaaca acaccagcaa
1921 gacctgcagc aagtgcgggc acctcaacaa ctacttcaac ttcgagtacc ggaagaagaa
1981 caagttccca cacttcaagt gcgagaagtg caactttaag gagaacgccg attacaacgc
2041 cgccctgaac atcagcaacc ctaagctgaa gagcactaag gaggagccca aaaggccggc
2101 ggccacgaaa aaggccggcc aggcaaaaaa gaaaaaggga agcggagcta ctaacttcag
2161 cctgctgaag caggctggag acgtggagga gaaccctgga cctatggtga gcaagggcga
2221 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
2281 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
2341 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
2401 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
2461 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgatga
2521 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
2581 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaaaaccat
2641 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
2701 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
2761 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
2821 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
2881 ctccaccggc ggcatggacg agctgtacaa gtaagaattc ctagagctcg ctgatcagcc
2941 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
3001 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
3061 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
3121 gattgggaag agaatagcag gcatgctggg gagcggccgc aggaacccct agtgatggag
3181 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc
3241 cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag
3301 gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt
3361 caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
3421 cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc
3481 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
3541 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
3601 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
3661 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct
3721 attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga
3781 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca
3841 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
3901 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
3961 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
4021 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
4081 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
4141 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
4201 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
4261 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
4321 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
4381 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
4441 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
4501 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
4561 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
4621 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
4681 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
4741 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
4801 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
4861 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
4921 ccggtgagcg tggaagccgc ggtatcattg cagcactggg gccagatggt aagccctccc
4981 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
5041 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
5101 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
5161 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
5221 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
5281 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
5341 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
5401 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
5461 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
5521 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
5581 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
5641 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
5701 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
5761 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
5821 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
5881 ggccttttgc tcacatgtga gggcctattt cccatgattc cttcatattt gcatatacga
5941 tacaaggctg ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta
6001 caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt
6061 tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta
6121 tatatcttgt ggaaaggacg aaacaccggg cttcactgat aaagtggaga accgcttcac
6181 caaaagctgt cccttagggg attagaactt gagtgaaggt gggctgcttg catcagccta
6241 atgtcgagaa gtgctttctt cggaaagtaa ccctcgaaac aaattcattt gaatgaagga
6301 atgcaacatc ggatatggta agacatgttt ttttctagag gtacccgtta cataacttac
6361 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caatagtaac
6421 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
6481 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
6541 atggcccgcc tggcattgtg cccagtacat gaccttatgg gactttccta cttggcagta
6601 catctacgta ttagtcatcg ctattaccat ggtcgaggtg agccccacgt tctgcttcac
6661 tctccccatc tcccccccct ccccaccccc aattttgtat tta
//
