| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-PHRF1-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001519 |
| 产品用途: | 3' AAVLINK plasmid for PHRF1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | PHRF1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-PHRF1-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-PHRF1-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-PHRF1-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-PHRF1-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-PHRF1-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-PHRF1-FLAG 6491 bp DNA circular SYN 17-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6491)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 14, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6491
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3204
/codon_start=1
/label=PHRF1
/translation="GAQAGPLGPQPEDVPVAVGEPWQLFL*AL*E*EEEEKEISVQTSG
KGVLPHQQPGEALQAQASAGTQPRAARQEGECGVAPRPEEAEVPVPKLGAQGTGAQAAS
VP*EVAADPVPFPREEGGCEGGFPSTPCTGGARAGRPPHQVASLGGSTCLAGGGYGRQG
PPAGSPCPGGGS*V*AGRPGPGLWRLRGGRTRL**FLKRRRFHPAR*HELATFSRKHRL
FPRARLPTEACVAPSQPGRGRHPEGGVIDAR*RPFAAPTPARGHPGATFAQAGRG*EG*
GTQFPGCGACGEGRQPLCEWEGTGGSPA*GGGFADPPAAVQSPGEAGHLEPAGVGEQRP
RRGQSPPGTTSQATEAPRRSLGHGGCGPHRGQAGVLRAALSQSRASGTRVPRHRPLSGL
QPRPAACPGPALKHPTLRTGQPAHGPVHPSGEPAASGLWGSTDPGPSARCPDPSLRASQ
SSHCSQQLGGEDPGPQASCGENQEGGVHEEAAHAGACCGGGEAGHQALLPEEGGDQGGV
QGHPAQGRAEDLPQQEWRDQPREGGQPGEGVRGQVQAHAQAQETRGRGGAAHAGGRG"
misc_feature 2393..2398
/label=SDS
misc_feature 2411..2416
/label=SDS
misc_feature 2927..2932
/label=SDS
misc_feature 2972..2977
/label=SDS
misc_feature 3023..3028
/label=SDS
CDS 3211..3276
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3280..3711
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3424..3429
/label=SDS
repeat_region 3754..3894
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3754..3883
/label=AAV2 ITR
rep_origin 3969..4424
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4706..4810
/gene="bla"
/label=AmpR promoter
CDS 4811..5671
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5842..6430
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca gggagcacag gcggggcccc
1501 tggggccaca gccggaggac gtcccggtcg cggtcgggga gccctggcag ctcttcctat
1561 gagcactatg agagtaggaa gaagaagaaa aggagatcag cgtccagacc tcggggaagg
1621 gagtgctccc ccaccagcag cctggagagg ctctgcaggc acaagcatca gcgggaacgc
1681 agccacgagc ggccagacag gaaggagagt gtggcgtggc cccgagaccg gaggaagcgg
1741 aggtcccggt ccccaagctc ggagcacagg gcacgggagc acaggcggcc tcggtcccgt
1801 gagaagtggc cgcagacccg gtcccattcc ccagagagga agggggctgt gagggaggct
1861 tccccagcac cccttgcaca gggggagcca gggcgggaag acctccccac caggttgcca
1921 gccttggggg aagcacatgt ctcgccggag gtggctacgg ccgacaaggc ccccctgcag
1981 gctccccctg tcctggaggt ggcagctgag tgtgagccgg acgacctgga cctggattat
2041 ggcgactccg tggaggccgg acacgtcttt gatgatttct caagcgacgc cgttttcatc
2101 cagctcgatg acatgagctc gccaccttct cccgaaagca cagactcttc cccagagcga
2161 gacttcccac tgaagcctgc gttgccccca gccagcctgg ccgtggccgc catccagagg
2221 gaggtgtcat tgatgcacga tgaagaccct tcgcagcccc cacccctgcc agagggcacc
2281 caggagccac atttgctcag gccggacgcg gctgagaagg ctgaggcacc cagttccccg
2341 gatgtggcgc ctgcggggaa ggaagacagc ccctctgcga gtgggagggt acaggaggca
2401 gcccggcctg aggaggtggt ttcgcagacc cccctgctgc ggtccagagc cctggtgaag
2461 cgggtcacct ggaacctgca ggagtcggag agcagcgccc ccgccgagga cagagccccc
2521 cgggcaccac ttcacaggcc acagaagccc cgagaaggag cctgggacat ggaggatgtg
2581 gcccccacag gggtcaggca ggcgttctcc gagctgccct ttcccagtca cgtgcttccg
2641 gaacccgggt tcccagacac agacccctct caggtttaca gccccggcct gccgcctgcc
2701 ccggcccagc cctcaagcat cccaccctgc gcactggtca gccagcccac ggtccagttc
2761 atccttcagg ggagcctgcc gctagtgggc tgtggggcag cacagaccct ggccccagtg
2821 cccgctgccc tgaccccagc ctcagagcca gccagtcaag ccactgcagc cagcaactcg
2881 gaggagaaga ccccggcccc caggctagct gcggagaaaa ccaagaagga ggagtacatg
2941 aagaagctgc acatgcagga gcgtgctgtg gaggaggtga agctggccat caagcccttc
3001 taccagaaga gggaggtgac caaggaggag tacaaggaca tcctgcgcaa ggccgtgcag
3061 aagatctgcc acagcaagag tggagagatc aaccccgtga aggtggccaa cctggtgaag
3121 gcgtacgtgg acaagtacag gcacatgcgc aggcacaaga aaccagaggc cggggaggag
3181 ccgcccacgc agggggccga gggctgtaca gactacaaag accatgacgg tgattataaa
3241 gatcatgaca tcgactacaa ggatgacgat gacaagtaac tcgagataat caacctctgg
3301 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
3361 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
3421 tctcctcctt gtataaatcc tggttagttc ttgccacggc ggaactcatc gccgcctgcc
3481 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgtttattt
3541 gtgaaatttg tgatgctatt gctttatttg taaccatcta gctttatttg tgaaatttgt
3601 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
3661 tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggtttttta aagatctgga
3721 gaccggtgtg gcacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca
3781 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc
3841 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc
3901 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca
3961 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
4021 cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt
4081 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
4141 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg
4201 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
4261 taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt
4321 tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca
4381 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag
4441 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
4501 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
4561 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
4621 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
4681 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
4741 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
4801 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
4861 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
4921 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
4981 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
5041 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
5101 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
5161 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
5221 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
5281 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
5341 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
5401 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
5461 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
5521 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
5581 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
5641 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
5701 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
5761 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
5821 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
5881 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
5941 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
6001 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
6061 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
6121 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
6181 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
6241 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
6301 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
6361 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
6421 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
6481 tgctcacatg t
//
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