| 质粒名称: | pAAVLINK-EF1a-HA-5'-SETD5 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001443 |
| 产品用途: | 5' AAVLINK plasmid for SETD5 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | SETD5 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINK-EF1a-HA-5'-SETD5载体质粒基本信息
pAAVLINK-EF1a-HA-5'-SETD5质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-SETD5载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-SETD5质粒载体简介
pAAVLINK-EF1a-HA-5'-SETD5质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-SETD5 5945 bp DNA circular SYN 13-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5945)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 8, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5945
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 146..688
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 175..386
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 399..667
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 493..498
/label=SDS
CDS 704..730
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 737..3107
/codon_start=1
/label=SETD5
/translation="SIAIPLGVTTSDTSYSDMAAGSDPESVEASPAVNEKSVYSTHNYG
TTQRHGCRGLPYATIIPRSDLNGLPSPVEERCGDSPNSEGETVPTWCPCGLSQDGFLLN
CDKCRGMSRGKVIRLHRRKQDNISGGDSSATESWDEELSPSTVLYTATQHTPTSITLTV
RRTKPKKRKKSPEKGRAAPKTKKIKNSPSEAQNLDENTTEGWENRIRLWTDQYEEAFTN
QYSADVQNALEQHLHSSKEFVGKPTILDTINKTELACNNTVIGSQMQLQLGRVTRVQKH
RKILRAARDLALDTLIIEYRGKVMLRQQFEVNGHFFKKPYPFVLFYSKFNGVEMCVDAR
TFGNDARFIRRSCTPNAEVRHMIADGMIHLCIYAVSAITKDAEVTIAFDYEYSNCNYKV
DCACHKGNRNCPIQKRNPNATELPLLPPPPSLPTIGAETRRRKARRKELEMEQQNEASE
ENNDQQSQEVPEKVTVSSDHEEVDNPEEKPEEEKEEVIDDQENLAHSRRTREDRKVEAI
MHAFENLEKRKKRRDQPLEQSNSDVEITTTTSETPVGEETKTEAPESEVSNSVSNVTIP
STPQSVGVNTRRSSQAGDIAAEKLVPKPPPAKPSRPRPKSRISRYRTSSAQRLKRQKQA
NAQQAELSQAALEEGGSNSLVTPTEAGSLDSSGENRPLTGSDPTVVSITGSHVNRAASK
YPKTKKYLVTEWLNDKAEKQECPVECPLRITTDPTVLATTLNMLPGLIHSPLICTTPKH
YIRFGSPFIPERRRRPLLPDGTFSSCKKRWIKQALEE"
misc_feature 2015..2020
/label=SDS
misc_feature 2252..2257
/label=SDS
intron 3108..3148
/label=Splicing Donor
misc_feature 3152..3185
/label=lox2272-JT1571
repeat_region 3230..3348
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3230..3337
/label=AAV2 ITR
rep_origin 3423..3878
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4160..4264
/gene="bla"
/label=AmpR promoter
CDS 4265..5125
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5296..5884
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
721 tgactatgct ggatctagca ttgcaatccc tctgggagtc accacatcag atacatccta
781 ctcagatatg gctgctggat cagaccctga atctgtggag gctagtccag cagttaatga
841 gaagagcgtg tattccactc ataattatgg gaccactcag aggcatgggt gtcgaggact
901 gccttatgct acgatcatcc ctcgttctga cctgaatggc ctgccgtcgc ctgtagagga
961 acgctgtgga gacagcccga actctgaagg agaaactgta cctacctggt gtccttgtgg
1021 tctttctcag gatggcttcc ttctcaactg tgacaagtgc aggggaatga gcagggggaa
1081 ggttattaga cttcatcggc ggaagcagga caacatatca ggtggggata gcagtgcaac
1141 agaaagctgg gatgaggagc tttctccttc cactgtgttg tatacagcaa cacagcacac
1201 acctacaagc atcaccttaa ctgttagaag aaccaaaccc aagaagcgga aaaagagtcc
1261 agaaaagggt cgtgcagcac caaagacgaa gaaaatcaag aattctccct ctgaagcaca
1321 gaatttagat gagaatacaa ctgagggctg ggaaaatcgg ataagactat ggactgacca
1381 gtatgaagaa gctttcacta atcagtacag tgcagatgta cagaacgcgc ttgaacaaca
1441 cctacattct agcaaggaat ttgtgggcaa acctactatt ttagacacta ttaataagac
1501 tgaattggcc tgtaataaca cagttattgg ttcccaaatg cagttacagc tgggaagagt
1561 cactcgtgtt caaaagcacc ggaagatcct gagggctgca agagatttgg ctttggacac
1621 tcttataata gagtatcgtg ggaaagtcat gttacgacag caatttgagg tcaatgggca
1681 tttcttcaaa aaaccatacc cctttgtgct cttctactca aaattcaatg gtgtagagat
1741 gtgtgtggat gcccgtactt tcggtaatga tgctcggttc atcagaagat catgtacacc
1801 aaatgcagag gtgcgacaca tgattgcaga tgggatgatt cacctgtgca tctatgctgt
1861 gtctgccatc accaaggatg ctgaggtcac catagcattt gattatgagt atagtaactg
1921 taattataaa gtggactgtg cctgtcacaa gggaaaccgg aattgtccta tacaaaaaag
1981 gaatcctaat gctacagaac tgccactcct accacctcct ccaagcctac ccaccattgg
2041 agcagagact agacgtagaa aagcacgacg gaaagagcta gagatggagc agcagaatga
2101 ggcttcagag gagaataatg accagcaatc acaagaagtt ccagaaaaag taactgtatc
2161 cagtgatcat gaggaagtag acaatccaga agaaaaacca gaagaagaga aagaagaggt
2221 tatagatgac caggagaacc tagctcatag caggaggacc agggaagata gaaaggtaga
2281 agccatcatg catgcttttg aaaacttaga gaaaagaaag aagcggcggg atcagccctt
2341 ggaacagagc aactctgatg tagagattac tacaaccacc tcagagactc ctgttggtga
2401 agagacaaaa actgaagccc ctgaatctga agttagcaac tctgtttcaa atgttaccat
2461 cccaagcacc ccacagagtg ttggtgtgaa tacccggagg tcttcccaag caggggatat
2521 tgctgcagaa aaactagtcc ccaagccacc tccagcaaag ccttctaggc cccggccgaa
2581 gagtcgaatt tctcggtaca ggaccagttc agcccaaaga ctaaagcgtc agaagcaggc
2641 caatgcacag caggcagaat tgtcacaagc tgccttggaa gagggaggaa gtaacagttt
2701 agtaactcct actgaagctg gaagtctaga cagttcagga gaaaacaggc cattaacagg
2761 gtctgaccca actgtggtgt caattactgg atcccatgtc aaccgtgctg catctaaata
2821 ccccaaaacc aaaaagtatc tagttacaga atggttgaat gacaaagcag agaagcaaga
2881 gtgccctgtt gagtgccctt tacgtatcac aacggatcca actgtactgg caacgaccct
2941 aaacatgtta ccaggtctta tccattcccc gttaatttgc accaccccca aacactacat
3001 tcgctttggc tcacccttta tccctgagag acgtcgaagg ccccttctgc ctgatggcac
3061 attcagctcc tgtaagaagc gctggataaa acaagcctta gaagaaggta agtatcaagg
3121 ttacaagaca ggtttaagga gaccaataag ctattattcg tataggatac tttatacgaa
3181 gttattaagt tatggagacc ggtgtggcac gtgcggaccg agcggccgca ggaaccccta
3241 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca
3301 aaggtcgccc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc
3361 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata
3421 gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
3481 cgctacactt gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
3541 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
3601 tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg
3661 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
3721 tggactcttg ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt
3781 ataagggatt ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt
3841 taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat
3901 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
3961 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
4021 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
4081 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
4141 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
4201 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
4261 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
4321 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
4381 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
4441 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
4501 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
4561 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
4621 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
4681 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
4741 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
4801 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
4861 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
4921 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
4981 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
5041 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
5101 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
5161 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
5221 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
5281 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
5341 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
5401 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
5461 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
5521 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
5581 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
5641 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
5701 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
5761 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
5821 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
5881 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
5941 catgt
//
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pAAVLINK-EF1a-HA-5'-SETD5质粒载体应用举例
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