pEMTB-PSmOrange3

LOCUS       pEMTB-PSmOrange3               5543 bp DNA     circular SYN 20-NOV-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported Jul 21, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..5543
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      271..280
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      271..280
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             280..309
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      1129..1138
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1129..1138
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1135..1842
                     /codon_start=1
                     /product="enhanced orange to far-red photoswitchable 
                     monomeric fluorescent protein (Subach et al., 2012)"
                     /label=PSmOrange3
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGTVNGHEFEVEGKGEGHPYEG
                     FQTVKLKVTKGGPLPFAWDILSPLVTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKMRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEVRMRLKLKDGGHYTSEAKTTYKAKKSVLLPGAYIVGIKLDITSHNEDFTIVEQYERS
                     EARHSTGGKDELYK"
     polyA_signal    1967..2088
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2095..2550)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2577..2681
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2683..3040
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3075..3869
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    4101..4148
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4477..5065
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     enhancer        5240..5543
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
      241 actcagatct cgagctcaag cttcgaattc gccaccatgg agcagaagct catctcagaa
      301 gaagacctcg gcgcgccagt gcgaagcgaa acagcacccg acagctacaa agtgcaagat
      361 aagaaaaatg cctccagccg ccctgcctct gcaatttcag gacaaaataa caaccactca
      421 ggaaataaac cagaccctcc gcctgtgtta cgtgttgatg accggcagcg gctggcccgg
      481 gagcgacgtg aggaacggga gaaacagcta gctgcaagag aaatagtgtg gttagaaaga
      541 gaagagcgag ccaggcagca ctacgagaag cacctggaag agcggaagaa gaggttggag
      601 gagcagaggc agaaggagga gcggaggagg gctgctgtgg aggagaagcg gaggcagaga
      661 cttgaggagg acaaagaacg ccacgaagct gttgtacggc gcacaatgga aaggagccag
      721 aagccaaaac agaagcataa ccgttggtcg tggggaggct ctctccatgg gagccctagc
      781 atccacagtg cagatccaga caggcggtca gtttccacca tgaatctttc gaaatatgtt
      841 gatcccgtca ttagcaagcg gctctcctct tcatctgcaa ctttactaaa ttctccagat
      901 agagctcgcc gcctgcagct cagcccatgg gagagcagcg ttgttaacag actcctgacg
      961 cccacacatt cgttcctggc cagaagtaaa agcacagctg ccttgtctgg agaagcagca
     1021 tcttgcagcc ccatcatcat gccctacaaa gctgcacact ctagaaattc gatggatcga
     1081 ccaaaactct ttgtaacacc acctgagggc tcttcggatc caccggtcgc caccatggtg
     1141 agcaagggcg aggagaataa tatggccatc attaaggagt tcatgcgctt caaggtgcac
     1201 atggagggca ctgtgaacgg ccacgagttc gaggtcgagg gcaagggcga gggccacccc
     1261 tacgagggct ttcagaccgt taagctgaag gtgaccaagg gtggccccct gcccttcgcc
     1321 tgggacatcc tgtcccctct cgtcacctac ggctccaagg cgtacgtgaa gcaccccgcc
     1381 gacatccccg actacttcaa gctgtccttc cccgagggat tcaagtggga gcgtgtgatg
     1441 aactacgagg acggcggcgt ggtgaccgtg acccaggact cctctctgca ggacggcgag
     1501 ttcatctaca aggtgaagat gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
     1561 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
     1621 aagggcgagg tcaggatgag gctgaagctg aaggatggcg gccactacac ctccgaggcc
     1681 aagactacct acaaggccaa gaagtccgtg ctgctgcccg gcgcctacat cgtcggcatc
     1741 aagttggaca ttacctcaca caacgaggac ttcaccatcg tggaacagta tgaacgcagc
     1801 gaggcccgcc actccaccgg cggcaaggac gagctgtaca agtaagcggc cgcgactcta
     1861 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     1921 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     1981 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     2041 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaag gcgtaaattg
     2101 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     2161 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     2221 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     2281 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     2341 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     2401 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     2461 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     2521 ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg
     2581 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     2641 aaccctgata aatgcttcaa taatattgaa aaaggaagag tcctgaggcg gaaagaacca
     2701 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     2761 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     2821 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     2881 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     2941 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     3001 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat
     3061 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     3121 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     3181 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     3241 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     3301 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     3361 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     3421 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     3481 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     3541 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     3601 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     3661 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     3721 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     3781 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     3841 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca
     3901 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt
     3961 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
     4021 gctggagttc ttcgcccacc ctagggggag gctaactgaa acacggaagg agacaatacc
     4081 ggaaggaacc cgcgctatga cggcaataaa aagacagaat aaaacgcacg gtgttgggtc
     4141 gtttgttcat aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag
     4201 accccattgg ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc
     4261 gggtgaaggc ccagggctcg cagccaacgt cggggcggca ggccctgcca tagcctcagg
     4321 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     4381 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     4441 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     4501 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     4561 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     4621 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     4681 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     4741 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     4801 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     4861 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     4921 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     4981 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     5041 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     5101 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     5161 ccgtattacc gccatgcatt agttattaat agtaatcaat tacggggtca ttagttcata
     5221 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     5281 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     5341 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     5401 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     5461 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     5521 tattagtcat cgctattacc atg
//