pTCK303-bk1-mCherry-bk2

LOCUS       pTCK303-bk1-mCherry-bk2          16080 bp DNA     circular SYN 09-SEP-2026
DEFINITION  2112 (303-bk1-mCherry-bk2) actual sequencing consensus - features 
            remapped from original SnapGene map.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 22, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..16080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             77..1102
                     /codon_start=1
                     /label=HygR
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    1143..1317
                     /label=CaMV poly(A) signal
     misc_feature    1395..1419
                     /label=LB T-DNA repeat
     CDS             1844..2638
                     /codon_start=1
                     /label=KanR
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      3924..4512
                     /direction=RIGHT
                     /label=ori
     misc_feature    4698..4838
                     /label=bom
     CDS             complement(6944..7573)
                     /codon_start=1
                     /label=pVS1 StaA
                     /translation="SNEF*FNFSGQRLDLAGSVALGF*FKNGCAGGGSAWVAHALRDTG
                     LKNGQLVPGQRLGNLTADARAFDRPRHDKGRL*PSIRDLNALLNQLHQVGGGPYVVRAW
                     LHRNQHEVGCLDRGHSQVRRLGRSVDHYEVAPADGLHVAVNRRAVDADNG*RLIFPHGR
                     PIAGTALGIGID*QNIGPGELQGAG*MGCDGRLA*PAFLVKYSDNLH"
     misc_feature    8874..8898
                     /label=RB T-DNA repeat
     terminator      8920..9172
                     /label=NOS terminator
     CDS             complement(9207..9224)
                     /codon_start=1
                     /label=6xHis
                     /translation="VVVVVV"
     CDS             complement(join(9231..11048,11239..11253))
                     /codon_start=1
                     /label=GUS
                     /translation="LFASLLRFFTEVHASPAFLQQKSRRLRFAVASEDPFLVTANAQYA
                     LRGRKIGEIPYLFTDDGADAIKDAVIHIQPCTLILFTPHVGVH*VQPG*RIHAVFGDDN
                     RLMQFLLPGQKFFFQYLLCRFQIAALDIPSVITVQAQHIKEIADGIGVSVAEHYIDAGD
                     RTRRVEFTRCFRQWREIFPCTLRTGIRFVGNTPHHHAWVVFVTRYQLFNRL*VRLLSFP
                     VDCLFAVQFFRLVARFETNA*REVKADSSSFINHHDAMFICPVEHLFSVRVMRGTVGVG
                     PNPVH*CVVVHHQHVIESFATQVRIFMTTKASKVERFVVNQELFALHCH*PDADAKRVD
                     ITLCLAFGCDAQFIEITFTRLPEVRIHHLQSPASALSSCNHLLIRITQFNADITIGHHL
                     PVNRRVVTVLRDMRHHGDIVHPGVRRGVEHYAAMDSGIVKEIMEVRLLFLAVFVGNHHS
                     RRDSLPVQFVVHTNGDTYTFPGNNIRRDIGFKWRIAALMLHHFLIIDPHFAVMSDRIET
                     QHDTLACPTFRYKDFALIPDVARIITNICIGELIVKTAWHSNCPAFL*RAFPPTLINST
                     VFAIQTECPQAVEFFDFTGWGFYRTDESSVPQIYH"
     intron          11049..11238
                     /label=cat1 intron
     promoter        complement(11270..11615)
                     /label=CaMV 35S promoter
     primer_bind     11992..12008
                     /label=M13 fwd
     promoter        12024..14016
                     /label=Ubi promoter
     misc_feature    13933..13950
                     /label=303F
     CDS             14065..14769
                     /codon_start=1
                     /label=mCherry
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     misc_feature    14776..14796
                     /label=同源臂
     misc_feature    14809..14829
                     /label=同源臂
     CDS             14830..14839
                     /codon_start=1
                     /label=Feature 22
                     /translation="EFP"
     terminator      14840..15092
                     /label=NOS terminator
     misc_feature    14889..14908
                     /label=NOSR
     primer_bind     complement(15109..15125)
                     /label=M13 rev
     protein_bind    15133..15149
                     /label=lac operator
     promoter        complement(join(15157..16080,1..1256))
                     /label=lac promoter
     protein_bind    15202..15223
                     /label=CAP binding site
     promoter        join(15414..16080,1..10)
                     /label=CaMV 35S promoter (enhanced)
ORIGIN
        1 gacacgctga aatcaccagt ctctctctac aaatctatct ctctcgagct ttcgcagatc
       61 cggggggcaa tgagatatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct
      121 gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg
      181 tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga
      241 tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc
      301 ggaagtgctt gacattgggg agtttagcga gagcctgacc tattgcatct cccgccgttc
      361 acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc tacaaccggt
      421 cgcggaggct atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc
      481 attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc
      541 tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc
      601 gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt
      661 gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat
      721 tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg
      781 gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga
      841 gcttgcagga tcgccacgac tccgggcgta tatgctccgc attggtcttg accaactcta
      901 tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc
      961 aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc
     1021 cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac
     1081 tcgtccgagg gcaaagaaat agagtagatg ccgaccggga tctgtcgatc gacaagctcg
     1141 agtttctcca taataatgtg tgagtagttc ccagataagg gaattagggt tcctataggg
     1201 tttcgctcat gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac
     1261 ttctatcaat aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatcccc
     1321 cgaattaatt cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt
     1381 ctaagcgtca atttgtttac accacaatat atcctgccac cagccagcca acagctcccc
     1441 gaccggcagc tcggcacaaa atcaccactc gatacaggca gcccatcagt ccgggacggc
     1501 gtcagcggga gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga
     1561 agaacggcaa ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt
     1621 gattgtaacg atgacagagc gttgctgcct gtgatcaccg cggtttcaaa atcggctccg
     1681 tcgatactat gttatacgcc aactttgaaa acaactttga aaaagctgtt ttctggtatt
     1741 taaggtttta gaatgcaagg aacagtgaat tggagttcgt cttgttataa ttagcttctt
     1801 ggggtatctt taaatactgt agaaaagagg aaggaaataa taaatggcta aaatgagaat
     1861 atcaccggaa ttgaaaaaac tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat
     1921 gtctcctgct aaggtatata agctggtggg agaaaatgaa aacctatatt taaaaatgac
     1981 ggacagccgg tataaaggga ccacctatga tgtggaacgg gaaaaggaca tgatgctatg
     2041 gctggaagga aagctgcctg ttccaaaggt cctgcacttt gaacggcatg atggctggag
     2101 caatctgctc atgagtgagg ccgatggcgt cctttgctcg gaagagtatg aagatgaaca
     2161 aagccctgaa aagattatcg agctgtatgc ggagtgcatc aggctctttc actccatcga
     2221 catatcggat tgtccctata cgaatagctt agacagccgc ttagccgaat tggattactt
     2281 actgaataac gatctggccg atgtggattg cgaaaactgg gaagaagaca ctccatttaa
     2341 agatccgcgc gagctgtatg attttttaaa gacggaaaag cccgaagagg aacttgtctt
     2401 ttcccacggc gacctgggag acagcaacat ctttgtgaaa gatggcaaag taagtggctt
     2461 tattgatctt gggagaagcg gcagggcgga caagtggtat gacattgcct tctgcgtccg
     2521 gtcgatcagg gaggatatcg gggaagaaca gtatgtcgag ctattttttg acttactggg
     2581 gatcaagcct gattgggaga aaataaaata ttatatttta ctggatgaat tgttttagta
     2641 cctagggaag gtgcgaataa gcggggaaat tcttctcggc tgactcagtc atttcatttc
     2701 ttcatgtttg agccgatttt ttctcccgta aatgccttga atcagcctat ttagaccgtt
     2761 tcttcgccat ttaaggcgtt atccccagtt tttagtgaga tctctcccac tgacgtatca
     2821 tttggtccgc ccgaaacagg ttggccagcg tgaataacat cgccagttgg ttatcgtttt
     2881 tcagcaaccc cttgtatctg gctttcacga agccgaactg tcgcttgatg atgcgaaatg
     2941 ggtgctccac cctggcccgg atgctggctt tcatgtattc gatgttgatg gccgttttgt
     3001 tcttgcgtgg atgctgtttc aaggttctta ccttgccggg gcgctcggcg atcagccagt
     3061 ccacatccac ctcggccagc tcctcgcgct gtggcgcccc ttggtagccg gcatcggctg
     3121 agacaaattg ctcctctcca tgcagcagat tacccagctg attgaggtca tgctcgttgg
     3181 ccgcggtggt gaccaggctg tgggtcaggc cactcttggc atcgacacca atgtgggcct
     3241 tcatgccaaa gtgccactga ttgcctttct tggtctgatg catctccgga tcgcgttgct
     3301 gctctttgtt cttggtcgag ctgggtgcct caatgatggt ggcatcgacc aaggtgcctt
     3361 gagtcatcat gacgcctgct tcggccagcc agcgattgat ggtcttgaac aattggcggg
     3421 ccagttgatg ctgctccagc aggtggcgga aattcatgat ggtggtgcgg tccggcaagg
     3481 cgctatccag ggataaccgg gcaaacagac gcatggaggc gatttcgtac agagcatctt
     3541 ccatcgcgcc atcgctcagg ttgtaccaat gctgcatgca gtgaatgcgt agcatggttt
     3601 ccagcggata aggtcgccgg ccattaccag ccttggggta aaacggctcg atgacttcca
     3661 ccatgttttg ccatggcaga atctgctcca tgcgggacaa gaaaatctct tttctggtct
     3721 gacggcgctt actgctgaat tcactgtcgg cgaaggtaag ttgatgactc atgatgaacc
     3781 ctgttctatg gctccagatg acaaacatga tctcatatca gggacttgtt cgcaccttcc
     3841 ctagaatgca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     3901 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     3961 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     4021 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     4081 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     4141 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     4201 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     4261 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     4321 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     4381 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     4441 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     4501 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     4561 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     4621 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     4681 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     4741 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     4801 acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg
     4861 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     4921 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagg
     4981 gtgccttgat gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt
     5041 agattgcctg gccgtaggcc agccattttt gagcggccag cggccgcgat aggccgacgc
     5101 gaagcggcgg ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg cagctcttcg
     5161 gctgtgcgct ggccagacag ttatgcacag gccaggcggg ttttaagagt tttaataagt
     5221 tttaaagagt tttaggcgga aaaatcgcct tttttctctt ttatatcagt cacttacatg
     5281 tgtgaccggt tcccaatgta cggctttggg ttcccaatgt acgggttccg gttcccaatg
     5341 tacggctttg ggttcccaat gtacgtgcta tccacaggaa agagaccttt tcgacctttt
     5401 tcccctgcta gggcaatttg ccctagcatc tgctccgtac attaggaacc ggcggatgct
     5461 tcgccctcga tcaggttgcg gtagcgcatg actaggatcg ggccagcctg ccccgcctcc
     5521 tccttcaaat cgtactccgg caggtcattt gacccgatca gcttgcgcac ggtgaaacag
     5581 aacttcttga actctccggc gctgccactg cgttcgtaga tcgtcttgaa caaccatctg
     5641 gcttctgcct tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga
     5701 tcgatcaaaa agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc
     5761 tcgcggtaca tccaatcagc tagctcgatc tcgatgtact ccggccgccc ggtttcgctc
     5821 tttacgatct tgtagcggct aatcaaggct tcaccctcgg ataccgtcac caggcggccg
     5881 ttcttggcct tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt
     5941 tctaccaggt cgtctttctg ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc
     6001 gcaacgtgtg gacggaacac gcggccgggc ttgtctccct tcccttcccg gtatcggttc
     6061 atggattcgg ttagatggga aaccgccatc agtaccaggt cgtaatccca cacactggcc
     6121 atgccggccg gccctgcgga aacctctacg tgcccgtctg gaagctcgta gcggatcacc
     6181 tcgccagctc gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat gatgctgcga
     6241 ctatcgcggg tgcccacgtc atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc
     6301 ttgggcggct tcctaatcga cggcgcaccg gctgccggcg gttgccggga ttctttgcgg
     6361 attcgatcag cggccgcttg ccacgattca ccggggcgtg cttctgcctc gatgcgttgc
     6421 cgctgggcgg cctgcgcggc cttcaacttc tccaccaggt catcacccag cgccgcgccg
     6481 atttgtaccg ggccggatgg tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc
     6541 cagtgccatt gcagggccgg caggcaaccc agccgcttac gcctggccaa ccgcccgttc
     6601 ctccacacat ggggcattcc acggcgtcgg tgcctggttg ttcttgattt tccatgccgc
     6661 ctcctttagc cgctaaaatt catctactca tttattcatt tgctcattta ctctggtagc
     6721 tgcgcgatgt attcagatag cagctcggta atggtcttgc cttggcgtac cgcgtacatc
     6781 ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga cccgcttcat ggctggcgtg
     6841 tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg gccggcactt
     6901 agcgtgtttg tgcttttgct cattttctct ttacctcatt aactcaaatg agttttgatt
     6961 taatttcagc ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt tctgattcaa
     7021 gaacggttgt gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg atacgggact
     7081 caagaatggg cagctcgtac ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc
     7141 ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct tccatccgtg acctcaatgc
     7201 gctgcttaac cagctccacc aggtcggcgg tggcccatat gtcgtaaggg cttggctgca
     7261 ccggaatcag cacgaagtcg gctgccttga tcgcggacac agccaagtcc gccgcctggg
     7321 gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg
     7381 tcgggcggtc gatgccgaca acggttagcg gttgatcttc ccgcacggcc gcccaatcgc
     7441 gggcactgcc ctggggatcg gaatcgacta acagaacatc ggccccggcg agttgcaggg
     7501 cgcgggctag atgggttgcg atggtcgtct tgcctgaccc gcctttctgg ttaagtacag
     7561 cgataacctt catgcgttcc ccttgcgtat ttgtttattt actcatcgca tcatatacgc
     7621 agcgaccgca tgacgcaagc tgttttactc aaatacacat caccttttta gacggcggcg
     7681 ctcggtttct tcagcggcca agctggccgg ccaggccgcc agcttggcat cagacaaacc
     7741 ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga acacgtaccc
     7801 ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc
     7861 ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct cggcggccgc cagggcgtcg
     7921 gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt gggcgtcact
     7981 tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc
     8041 gcctctttca cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc
     8101 ggggtgaggg tagggcgggg gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg
     8161 ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg caatgccggc gaacacggtc
     8221 aacaccatgc ggccggccgg cgtggtggtg tcggcccacg gctctgccag gctacgcagg
     8281 cccgcgccgg cctcctggat gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc
     8341 aggcggtcta gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg
     8401 gccagctccg ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag cttggtgcag
     8461 ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg
     8521 gcatagccca gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag
     8581 tcgcaagtat tctactttat gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc
     8641 agggcggcag cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc agaagacggc
     8701 tgcactgaac gtcagaagcc gactgcacta tagcagcgga ggggttggat caaagtactt
     8761 tgatcccgag gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     8821 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     8881 cgccaatata tcctgtcaaa cactgatagt ttaattcccg atctagtaac atagatgaca
     8941 ccgcgcgcga taatttatcc tagtttgcgc gctatatttt gttttctatc gcgtattaaa
     9001 tgtataattg cgggactcta atcataaaaa cccatctcat aaataacgtc atgcattaca
     9061 tgttaattat tacatgctta acgtaattca acagaaatta tatgataatc atcgcaagac
     9121 cggcaacagg attcaatctt aagaaacttt attgccaaat gtttgaacga tcggggaaat
     9181 tcgagctggt cacctgtaat tcacacgtgg tggtggtggt ggtggctagc ttgtttgcct
     9241 ccctgctgcg gtttttcacc gaagttcatg ccagtccagc gtttttgcag cagaaaagcc
     9301 gccgacttcg gtttgcggtc gcgagtgaag atccctttct tgttaccgcc aacgcgcaat
     9361 atgccttgcg aggtcgcaaa atcggcgaaa ttccatacct gttcaccgac gacggcgctg
     9421 acgcgatcaa agacgcggtg atacatatcc agccatgcac actgatactc ttcactccac
     9481 atgtcggtgt acattgagtg cagcccggct aacgtatcca cgccgtattc ggtgatgata
     9541 atcggctgat gcagtttctc ctgccaggcc agaagttctt tttccagtac cttctctgcc
     9601 gtttccaaat cgccgctttg gacataccat ccgtaataac ggttcaggca cagcacatca
     9661 aagagatcgc tgatggtatc ggtgtgagcg tcgcagaaca ttacattgac gcaggtgatc
     9721 ggacgcgtcg ggtcgagttt acgcgttgct tccgccagtg gcgcgaaata ttcccgtgca
     9781 ccttgcggac gggtatccgg ttcgttggca atactccaca tcaccacgct tgggtggttt
     9841 ttgtcacgcg ctatcagctc tttaatcgcc tgtaagtgcg cttgctgagt ttccccgttg
     9901 actgcctctt cgctgtacag ttctttcggc ttgttgcccg cttcgaaacc aatgcctaaa
     9961 gagaggttaa agccgacagc agcagtttca tcaatcacca cgatgccatg ttcatctgcc
    10021 cagtcgagca tctcttcagc gtaagggtaa tgcgaggtac ggtaggagtt ggccccaatc
    10081 cagtccatta atgcgtggtc gtgcaccatc agcacgttat cgaatccttt gccacgcaag
    10141 tccgcatctt catgacgacc aaagccagta aagtagaacg gtttgtggtt aatcaggaac
    10201 tgttcgccct tcactgccac tgaccggatg ccgacgcgaa gcgggtagat atcacactct
    10261 gtctggcttt tggctgtgac gcacagttca tagagataac cttcacccgg ttgccagagg
    10321 tgcggattca ccacttgcaa agtcccgcta gtgccttgtc cagttgcaac cacctgttga
    10381 tccgcatcac gcagttcaac gctgacatca ccattggcca ccacctgcca gtcaacagac
    10441 gcgtggttac agtcttgcgc gacatgcgtc accacggtga tatcgtccac ccaggtgttc
    10501 ggcgtggtgt agagcattac gctgcgatgg attccggcat agttaaagaa atcatggaag
    10561 taagactgct ttttcttgcc gttttcgtcg gtaatcacca ttcccggcgg gatagtctgc
    10621 cagttcagtt cgttgttcac acaaacggtg atacgtacac ttttcccggc aataacatac
    10681 ggcgtgacat cggcttcaaa tggcgtatag ccgccctgat gctccatcac ttcctgatta
    10741 ttgacccaca ctttgccgta atgagtgacc gcatcgaaac gcagcacgat acgctggcct
    10801 gcccaacctt tcggtataaa gacttcgcgc tgataccaga cgttgcccgc ataattacga
    10861 atatctgcat cggcgaactg atcgttaaaa ctgcctggca cagcaattgc ccggctttct
    10921 tgtaacgcgc tttcccacca acgctgatca attccacagt tttcgcgatc cagactgaat
    10981 gcccacaggc cgtcgagttt tttgatttca cgggttgggg tttctacagg acggacgagt
    11041 cgtcggttct gtaactatca tcatcatcat agacacacga aataaagtaa tcagattatc
    11101 agttaaagct atgtaatatt tacaccataa ccaatcaatt aaaaaataga tcagtttaaa
    11161 gaaagatcaa agctcaaaaa aataaaaaga gaaaagggtc ctaaccaaga aaatgaagga
    11221 gaaaaactag aaatttaccc tcagatctac catggtcaag agtcccccgt gttctctcca
    11281 aatgaaatga acttccttat atagaggaag ggtcttgcga aggatagtgg gattgtgcgt
    11341 catcccttac gtcagtggag atatcacatc aatccacttg ctttgaagac gtggttggaa
    11401 cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg
    11461 cagaggcatc ttcaacgatg gcctttcctt tatcgcaatg atggcatttg taggagccac
    11521 cttccttttc cactatcttc acaataaagt gacagatagc tgggcaatgg aatccgagga
    11581 ggtttccgga tattaccctt tgttgaaaag tctcaattgc cctttggtct tctgagactg
    11641 tatctttgat atttttggag tagacaagtg tgtcgtgctc caccatgttg acgaagattt
    11701 tcttcttgtc attgagtcgt aagagactct gtatgaactg ttcgccagtc tttacggcga
    11761 gttctgttag gtcctctatt tgaatctttg actccatgaa gctaaactga aggcgggaaa
    11821 cgacaatctg atccaagctc aagctgctct agcattcgcc attcaggctg cgcaactgtt
    11881 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
    11941 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
    12001 cggccagtgc caagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc ccctctctag
    12061 agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt tttgtcacac
    12121 ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact ctacgaataa
    12181 tataatctat agtactacaa taatatcagt gttttagaga atcatataaa tgaacagtta
    12241 gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt ttatcttttt
    12301 agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat acttcatcca
    12361 ttttattagt acatccattt agggtttagg gttaatggtt tttatagact aattttttta
    12421 gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact ctattttagt
    12481 ttttttattt aataatttag atataaaata gaataaaata aagtgactaa aaattaaaca
    12541 aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg agtagataat
    12601 gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa ccagcagcgt
    12661 cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct ggacccctct
    12721 cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg
    12781 gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg gcaccggcag
    12841 ctacggggga ttcctttccc accgctcctt cgctttccct tcctcgcccg ccgtaataaa
    12901 tagacacccc ctccacaccc tctttcccca acctcgtgtt gttcggagcg cacacacaca
    12961 caaccagatc tcccccaaat ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc
    13021 ctcccccccc ccccctctct accttctcta gatcggcgtt ccggtccatg gttagggccc
    13081 ggtagttcta cttctgttca tgtttgtgtt agatccgtgt ttgtgttaga tccgtgctgc
    13141 tagcgttcgt acacggatgc gacctgtacg tcagacacgt tctgattgct aacttgccag
    13201 tgtttctctt tggggaatcc tgggatggct ctagccgttc cgcagacggg atcgatttca
    13261 tgattttttt tgtttcgttg catagggttt ggtttgccct tttcctttat ttcaatatat
    13321 gccgtgcact tgtttgtcgg gtcatctttt catgcttttt tttgtcttgg ttgtgatgat
    13381 gtggtctggt tgggcggtcg ttctagatcg gagtagaatt ctgtttcaaa ctacctggtg
    13441 gatttattaa ttttggatct gtatgtgtgt gccatacata ttcatagtta cgaattgaag
    13501 atgatggatg gaaatatcga tctaggatag gtatacatgt tgatgcgggt tttactgatg
    13561 catatacaga gatgcttttt gttcgcttgg ttgtgatgat gtggtgtggt tgggcggtcg
    13621 ttcattcgtt ctagatcgga gtagaatact gtttcaaact acctggtgta tttattaatt
    13681 ttggaactgt atgtgtgtgt catacatctt catagttacg agtttaagat ggatggaaat
    13741 atcgatctag gataggtata catgttgatg tgggttttac tgatgcatat acatgatggc
    13801 atatgcagca tctattcata tgctctaacc ttgagtacct atctattata ataaacaagt
    13861 atgttttata attattttga tcttgatata cttggatgat ggcatatgca gcagctatat
    13921 gtggattttt ttagccctgc cttcatacgc tatttatttg cttggtactg tttcttttgt
    13981 cgatgctcac cctgttgttt ggtgttactt ctgcaggtcg actctcgagg atccggtacc
    14041 cggtatcgat ttaaaaggcc tatggtgagc aagggcgagg aggataacat ggccatcatc
    14101 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
    14161 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
    14221 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
    14281 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
    14341 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
    14401 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
    14461 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
    14521 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
    14581 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
    14641 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
    14701 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
    14761 ctgtacaaga ggcctcaggt cgactctcga ggatcaacta gtgagctcag tacccggtat
    14821 cgatttaaag aatttccccg atcgttcaaa catttggcaa taaagtttct taagattgaa
    14881 tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt
    14941 aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc
    15001 gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt
    15061 atcgcgcgcg gtgtcatcta tgttactaga tcgggaattc gtaatcatgt catagctgtt
    15121 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
    15181 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
    15241 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
    15301 ggggagaggc ggtttgcgta ttggctagag cagcttgcca acatggtgga gcacgacact
    15361 ctcgtctact ccaagaatat caaagataca gtctcagaag accaaagggc tattgagact
    15421 tttcaacaaa gggtaatatc gggaaacctc ctcggattcc attgcccagc tatctgtcac
    15481 ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca aatgccatca ttgcgataaa
    15541 ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc
    15601 acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga
    15661 tgtgaacatg gtggagcacg acactctcgt ctactccaag aatatcaaag atacagtctc
    15721 agaagaccaa agggctattg agacttttca acaaagggta atatcgggaa acctcctcgg
    15781 attccattgc ccagctatct gtcacttcat caaaaggaca gtagaaaagg aaggtggcac
    15841 ctacaaatgc catcattgcg ataaaggaaa ggctatcgtt caagatgcct ctgccgacag
    15901 tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac
    15961 cacgtcttca aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca
    16021 atcccactat ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag
//