| 质粒名称: | pKeratin-Electra2 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000214 |
| 产品用途: | Expresses Electra2 fused to C-terminus of human keratin |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | keratin, Electra2 |
| 物种来源: | Homo sapiens, Synthetic |
| 密码子优化: | 哺乳动物表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Dual-expression system for blue fluorescent protein optimization
Papadaki S., Wang X., Wang Y., Zhang H., Jia S., Liu S., Yang M., Zhang D., Jia J., Köster R.W., Namikawa K., Piatkevich K.D.
Sci. Reports 2022 121 12, 1–16 (2022)
|
pKeratin-Electra2
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pKeratin-Electra2载体质粒基本信息
pKeratin-Electra2质粒图谱载体图谱和pKeratin-Electra2载体序列质粒序列多克隆位点信息
pKeratin-Electra2质粒载体简介
pKeratin-Electra2质粒序列载体序列
LOCUS pKeratin-Electra2 5977 bp DNA circular SYN 29-MAY-2023
DEFINITION Keratin labeling with near-infrared fluorescent protein
miRFP670nano.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5977)
AUTHORS Oliinyk OS, Shemetov AA, Pletnev S, Shcherbakova DM, Verkhusha VV
TITLE Smallest near-infrared fluorescent protein evolved from
cyanobacteriochrome as versatile tag for spectral multiplexing.
JOURNAL Nat Commun. 2019 Jan 17;10(1):279. doi: 10.1038/s41467-018-08050-8.
PUBMED 30655515
REFERENCE 2 (bases 1 to 5977)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jun 30, 2026 from SnapGene 6.0.2
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat
Commun."; date: "2019-01-17"; volume: "10(1):279. doi"; pages: "
10.1038/s41467-018-08050-8"
FEATURES Location/Qualifiers
source 1..5977
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 86..389
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 390..593
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 625..1914
/codon_start=1
/label=Keratin
/translation="MSFTTRSTFSTNYRSLGSVQAPSYGARPVSSAASVYAGAGGSGSR
ISVSRSTSFRGGMGSGGLATGIAGGLAGMGGIQNEKETMQSLNDRLASYLDRVRSLETE
NRRLESKIREHLEKKGPQVRDWSHYFKIIEDLRAQIFANTVDNARIVLQIDNARLAADD
FRVKYETELAMRQSVENDIHGLRKVIDDTNITRLQLETEIEALKEELLFMKKNHEEEVK
GLQAQIASSGLTVEVDAPKSQDLAKIMADIRAQYDELARKNREELDKYWSQQIEESTTV
LTTQSAEVGAAETTLTELRRTVQSLEIDLDSMRNLKASLENSLREVEARYALQMEQLNG
ILLHLESELAQTRAEGQRQAQEYEALLNIKVKLEAEIATYRRLLEDGEDFNLGDALDSS
NSMQTIQKTTTRRIVDGKVVSETNDTKVLRH"
CDS 1939..2652
/codon_start=1
/label=Electra2
/translation="MVSKGEELIEENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
RIKVIEGGPLPFAFDILATSFLFGSKTFIKYPADIPDFFEQSFPEGFTWERVTRYEDGG
VVTVTQDTSLEDGGLVYNVKVRGVNFHSKGPVMQKKTEGWEPFTEMMYPADGGLRGYTD
IALKVDGGGHLHANIVTTYRSKKTVGNIKMPGVHAVDYRLERIEESDNETYVVLREVAV
AKYSNLGGGMDELFK"
polyA_signal 2790..2911
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(2918..3373)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3400..3504
/gene="bla"
/label=AmpR promoter
promoter 3506..3863
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3714..3849
/label=SV40 ori
/note="SV40 origin of replication"
CDS 3898..4692
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 4924..4971
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 5300..5888
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 ggataaccgt attaccgcca tgcattagtt attaatagta atcaattacg gggtcattag
61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
181 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
481 gtttgttttg gcaccaaaat caacgggact tttcaaaatg tcgtaacaac tccgccccat
541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
601 tgaaccgtca gatccgctag caccatgagc ttcaccactc gctccacctt ctccaccaac
661 taccggtccc tgggctctgt ccaggcgccc agctacggcg cccggccggt cagcagcgcg
721 gccagcgtct atgcaggcgc tgggggctct ggttcccgga tctccgtgtc ccgctccacc
781 agcttcaggg gcggcatggg gtccgggggc ctggccaccg ggatagccgg gggtctggca
841 ggaatgggag gcatccagaa cgagaaggag accatgcaaa gcctgaacga ccgcctggcc
901 tcttacctgg acagagtgag gagcctggag accgagaacc ggaggctgga gagcaaaatc
961 cgggagcact tggagaagaa gggaccccag gtcagagact ggagccatta cttcaagatc
1021 atcgaggacc tgagggctca gatcttcgca aatactgtgg acaatgcccg catcgttctg
1081 cagattgaca atgcccgtct tgctgctgat gactttagag tcaagtatga gacagagctg
1141 gccatgcgcc agtctgtgga gaacgacatc catgggctcc gcaaggtcat tgatgacacc
1201 aatatcacac gactgcagct ggagacagag atcgaggctc tcaaggagga gctgctcttc
1261 atgaagaaga accacgaaga ggaagtaaaa ggcctacaag cccagattgc cagctctggg
1321 ttgaccgtgg aggtagatgc ccccaaatct caggacctcg ccaagatcat ggcagacatc
1381 cgggcccaat atgacgagct ggctcggaag aaccgagagg agctagacaa gtactggtct
1441 cagcagattg aggagagcac cacagtgctc accacacagt ctgctgaggt tggagctgct
1501 gagacgacgc tcacagagct gagacgtaca gtccagtcct tggagatcga cctggactcc
1561 atgagaaatc tgaaggccag cttggagaac agcctgaggg aggtggaggc ccgctacgcc
1621 ctacagatgg agcagctcaa cgggatcctg ctgcaccttg agtcagagct ggcacagacc
1681 cgggcagagg gacagcgcca ggcccaggag tatgaggccc tgctgaacat caaggtcaag
1741 ctggaggctg agatcgccac ctaccgccgc ctgctggaag atggcgagga ctttaatctt
1801 ggtgatgcct tggacagcag caactccatg caaaccatcc aaaagaccac cacccgccgg
1861 atagtggatg gcaaagtggt gtctgagacc aatgacacca aagttctgag gcatcgaatt
1921 ctgcagtcga cggtacccat ggtgtctaag ggcgaagagc tgatcgagga aaatatgcgt
1981 atgaaggtgg tcatggaagg ttcggtcaac ggccaccaat tcaaatgcac aggtgaagga
2041 gaaggcagac cgtacgaggg agtgcaaacc atgaggatca aagtcatcga gggaggaccc
2101 ctgccatttg cctttgacat tcttgccacg tcgttcctgt tcggcagcaa gacctttatc
2161 aagtacccgg ccgacatccc tgatttcttc gaacagtcct ttcctgaggg ctttacttgg
2221 gaaagagtta cgagatacga agatggtgga gtagtcaccg tcacgcagga caccagcctt
2281 gaggatggtg ggctcgtcta caacgtcaag gtcagagggg taaactttca ctccaagggt
2341 cccgtgatgc agaagaagac cgagggttgg gagcctttca cagagatgat gtatccagca
2401 gatggtggtc tgagaggata taccgacatc gcactgaaag ttgatggtgg tggccatctg
2461 cacgccaaca tcgtgacaac ttacaggtca aaaaagaccg tcgggaacat caagatgccc
2521 ggtgtccatg ccgttgatta ccgcctggaa aggatcgaag agagtgacaa tgaaacctac
2581 gtagtgctaa gagaagtggc agttgccaaa tacagcaacc ttggtggtgg catggacgag
2641 ctgttcaagt aagatatcca gcacagtggc ggccgcgact ctagatcata atcagccata
2701 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
2761 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
2821 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
2881 gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg
2941 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
3001 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
3061 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
3121 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
3181 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
3241 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
3301 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
3361 ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
3421 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
3481 caataatatt gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc
3541 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
3601 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
3661 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
3721 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
3781 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
3841 ttggaggcct aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg
3901 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
3961 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
4021 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa
4081 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
4141 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
4201 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
4261 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
4321 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
4381 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc
4441 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
4501 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
4561 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
4621 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
4681 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
4741 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
4801 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
4861 accctagggg gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta
4921 tgacggcaat aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg
4981 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat
5041 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc
5101 tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt
5161 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
5221 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
5281 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
5341 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
5401 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
5461 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
5521 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
5581 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
5641 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
5701 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
5761 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
5821 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
5881 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
5941 ctcacatgtt ctttcctgcg ttatcccctg attctgt
//
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