| 质粒名称: | plenti-nCas9-BFP |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002239 |
| 产品用途: | Lentiviral vector used to express nCas9 with BFP selection marker. |
| 存储实验室: | Zhuobin Liang Lab 梁卓斌课题组 |
|
载体骨架基本信息 |
|
| 载体名称: | pLenti |
| 表达系统: | 慢病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | nCas9-BFP |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
TRU-PE: A Universal, Trackable Prime Editor Toolkit for Robust Single-and Multi-Locus Genome Engineering
Zhichao Qiu, Keke Sun, Qingwei Zeng, Ziwei Luo, Xinran Liu, Yaping Li, Xiang Lei, Ruilin Zhao, Zhen Zhang, Damia Romero-Moya, Yuan Yang, Xuan Wang, Ehsan Hashemi, Joe Z. Zhang, Xiaolong Wang, Alessandra Giorgetti, Zhuobin Liang
bioRxiv 2026.02.20.706938
|
买家导航
plenti-nCas9-BFP载体质粒基本信息
plenti-nCas9-BFP质粒图谱载体图谱和plenti-nCas9-BFP载体序列质粒序列多克隆位点信息
plenti-nCas9-BFP质粒载体简介
plenti-nCas9-BFP质粒序列载体序列
LOCUS plenti-nCas9-BFP 13740 bp DNA circular SYN 08-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13740)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 24, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..13740
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 47..66
/label=OL1919
primer_bind 228..256
/label=OL2624
primer_bind 231..256
/label=OL2675
enhancer 238..617
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
primer_bind 252..279
/label=OL1151
primer_bind complement(257..281)
/label=OL1047
primer_bind complement(319..346)
/label=OL1815
primer_bind 469..506
/label=OL1892
primer_bind complement(520..539)
/label=OL1815
primer_bind 539..558
/label=OL064
promoter 619..817
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 720..739
/label=OL790
primer_bind 766..787
/label=OL096
primer_bind 772..792
/label=OL391
primer_bind 772..791
/label=OL1335
primer_bind complement(773..790)
/label=OL400
primer_bind 773..790
/label=OL443
primer_bind 791..814
/label=OL152
primer_bind 791..814
/label=OL159
LTR 835..1015
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(1006..1025)
/label=OL898
misc_feature 1062..1187
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
primer_bind 1419..1439
/label=OL2370
primer_bind 1502..1521
/label=OL2348
primer_bind complement(1502..1521)
/label=OL2349
misc_feature 1680..1913
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
primer_bind complement(1835..1855)
/label=OL2371
CDS 2098..2142
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
primer_bind 2255..2277
/label=OL1570
primer_bind 2320..2339
/label=OL1050
primer_bind 2401..2420
/label=OL 5097
primer_bind complement(2423..2439)
/label=OL1168
misc_feature 2440..2557
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
primer_bind complement(2494..2513)
/label=OL1936
primer_bind complement(2500..2524)
/label=OL1571
primer_bind complement(2578..2607)
/label=OL 5080
primer_bind 2588..2623
/label=OL 5082
promoter 2608..3786
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
primer_bind 2608..2624
/label=OL2675
primer_bind 2608..2624
/label=OL2712
primer_bind 2627..2644
/label=OL2624
primer_bind complement(2726..2745)
/label=EF-1a
intron 2839..3777
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 2861..2878
/label=ef1a-seq1
primer_bind complement(3074..3093)
/label=OL 5118
primer_bind 3119..3132
/label=OL1324
primer_bind 3637..3656
/label=ef1a-seq2
primer_bind 3734..3754
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
primer_bind complement(3758..3811)
/label=OL2676
primer_bind complement(3787..3815)
/label=OL2625
primer_bind 3793..3812
/label=T7
/note="T7 promoter, forward primer"
promoter 3793..3811
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 3793..3811
/label=OL1124
primer_bind complement(3808..3827)
/label=OL1206
primer_bind complement(3855..3881)
/label=OL1013
primer_bind complement(3859..3881)
/label=OL1036
CDS 3861..3881
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
primer_bind 3862..3903
/label=OL1039
primer_bind 3862..3901
/label=OL1296
primer_bind complement(3862..3881)
/label=OL1298
CDS 3882..7982
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9(R221K N394K H840A)
/note="generates RNA-guided double strand breaks in DNA"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
primer_bind complement(3886..3905)
/label=OL1051
primer_bind 4110..4134
/label=OL2715
primer_bind complement(4110..4134)
/label=OL2716
primer_bind complement(4330..4349)
/label=OL1336
primer_bind 4350..4364
/label=OL1386
primer_bind complement(4418..4437)
/label=OL989
primer_bind complement(4490..4500)
/label=OL900
primer_bind complement(4523..4552)
/label=OL1321
primer_bind 4528..4557
/label=OL1322
primer_bind 4690..4704
/label=OL1270
primer_bind complement(5043..5077)
/label=OL1323
primer_bind 5043..5077
/label=OL1324
primer_bind 5174..5193
/label=OL1447
primer_bind complement(5377..5396)
/label=OL1446
primer_bind 5602..5616
/label=OL1271
primer_bind 5992..6011
/label=OL2835
primer_bind 6100..6134
/label=OL2384
primer_bind complement(6102..6134)
/label=OL1893
primer_bind 6348..6362
/label=OL1272
primer_bind complement(6381..6412)
/label=OL1316
primer_bind 6381..6412
/label=OL1317
primer_bind complement(6435..6454)
/label=OL1921
primer_bind 6526..6542
/label=OL2390
primer_bind 7455..7474
/label=OL2391
primer_bind 7685..7704
/label=OL1328
primer_bind 7685..7704
/label=OL1328a
CDS 7983..8060
/label=SGGSx2-sv40NLS-SGGSx2
/note="/created_by=chenp"
primer_bind 7983..8005
/label=OL1326
primer_bind 8022..8038
/label=OL1153
primer_bind complement(8030..8046)
/label=OL1154
primer_bind complement(8034..8060)
/label=OL1015
primer_bind 8039..8060
/label=OL1014
primer_bind 8040..8084
/label=OL1313
CDS 8040..8060
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
primer_bind complement(8045..8060)
/label=OL1040
CDS 8061..8117
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
primer_bind 8061..8117
/label=OL866
primer_bind complement(8061..8117)
/label=OL867
primer_bind complement(8061..8091)
/label=OL1019
primer_bind 8068..8081
/label=OL2447
primer_bind 8085..8118
/label=OL1687
primer_bind 8098..8143
/label=OL868
primer_bind complement(8098..8118)
/label=OL308
primer_bind 8099..8118
/label=OL309
CDS 8118..8813
/codon_start=1
/product="monomeric blue fluorescent protein"
/label=TagBFP
/note="mammalian codon-optimized"
/translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
LPSKLGHKLN"
primer_bind 8448..8468
/label=OL5119
primer_bind complement(8791..8816)
/label=OL1018
primer_bind complement(8792..8836)
/label=OL5084
primer_bind complement(8792..8819)
/label=OL1314
primer_bind complement(8792..8816)
/label=OL2711
primer_bind 8817..8840
/label=OL3078
primer_bind 8817..8839
/label=OL 5081
misc_feature 8832..9420
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(8933..8952)
/label=OL392
primer_bind complement(9087..9106)
/label=OL2270
CDS complement(9303..9314)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 9492..9725
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
primer_bind complement(9716..9755)
/label=OL898
polyA_signal 9757..9981
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind 9757..9776
/label=OL1407
primer_bind complement(9762..9781)
/label=OL1410
primer_bind complement(9801..9818)
/label=OL698
primer_bind complement(9803..9822)
/label=OL1938
primer_bind complement(9962..9981)
/label=OL1684
rep_origin 10027..10455
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 10469..10798
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind 10502..10539
/label=OL2448
primer_bind complement(10506..10543)
/label=OL2447
primer_bind 10510..10553
/label=OL2442
primer_bind 10574..10611
/label=OL2448
primer_bind complement(10578..10615)
/label=OL2447
primer_bind 10582..10625
/label=OL2442
rep_origin 10649..10784
/label=SV40 ori
/note="SV40 origin of replication"
promoter 10846..10893
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 10912..11286
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
primer_bind complement(11118..11136)
/label=OL900
primer_bind complement(11119..11136)
/label=PE004
primer_bind complement(11119..11136)
/label=OL1833
primer_bind complement(11119..11136)
/label=OL2710
primer_bind complement(11119..11136)
/label=OL3081
primer_bind 11120..11134
/label=OL2807
primer_bind complement(11123..11136)
/label=OL1205
primer_bind complement(11123..11136)
/label=OL1215
primer_bind complement(11124..11136)
/label=OL1146
primer_bind complement(11124..11136)
/label=OL2847
primer_bind complement(11125..11136)
/label=OL1038
polyA_signal 11416..11537
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(11501..11537)
/label=OL1698
primer_bind complement(11586..11602)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(11586..11602)
/label=OL390
protein_bind 11610..11626
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(11634..11664)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 11679..11700
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(11988..12576)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(12068..12087)
/label=OL1949
primer_bind complement(12170..12189)
/label=OL1041
primer_bind complement(12303..12322)
/label=OL631
primer_bind 12359..12378
/label=OL798
primer_bind complement(12600..12639)
/label=OL660
primer_bind 12620..12639
/label=OL659
CDS complement(12747..13607)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 13204..13223
/label=OL1922
primer_bind complement(13370..13389)
/label=OL1923
primer_bind 13370..13389
/label=OL1970
primer_bind 13523..13542
/label=OL1033
primer_bind complement(13523..13542)
/label=OL1034
promoter complement(13608..13712)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
2581 agatccagtt tggttaatta aggtaccggc tccggtgccc gtcagtgggc agagcgcaca
2641 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga
2701 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
2761 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
2821 tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg
2881 ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat
2941 cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct
3001 tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg
3061 tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga
3121 tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg
3181 cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc
3241 acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct
3301 caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg
3361 gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc
3421 cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca
3481 cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag
3541 taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta
3601 ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa
3661 gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga
3721 tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg
3781 tcgtgaggcc gctaatacga ctcactatag ggagagccgc caccatgaaa cggacagccg
3841 acggaagcga gttcgagtca ccaaagaaga agcggaaagt cgacaagaag tacagcatcg
3901 gcctggacat cggcaccaac tctgtgggct gggccgtgat caccgacgag tacaaggtgc
3961 ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag aagaacctga
4021 tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg aagagaaccg
4081 ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag atcttcagca
4141 acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc ttcctggtgg
4201 aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac gaggtggcct
4261 accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac agcaccgaca
4321 aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc cggggccact
4381 tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg ttcatccagc
4441 tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc ggcgtggacg
4501 ccaaggccat cctgtctgcc agactgagca agagcagaaa gctggaaaat ctgatcgccc
4561 agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg agcctgggcc
4621 tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg cagctgagca
4681 aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac cagtacgccg
4741 acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac atcctgagag
4801 tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga tacgacgagc
4861 accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct gagaagtaca
4921 aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac ggcggagcca
4981 gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac ggcaccgagg
5041 aactgctcgt gaagctgaag agagaggacc tgctgcggaa gcagcggacc ttcgacaacg
5101 gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg cggcaggaag
5161 atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg accttccgca
5221 tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg atgaccagaa
5281 agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag ggcgcttccg
5341 cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac gagaaggtgc
5401 tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg accaaagtga
5461 aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag aaaaaggcca
5521 tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg aaagaggact
5581 acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa gatcggttca
5641 acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag gacttcctgg
5701 acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca ctgtttgagg
5761 acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac gacaaagtga
5821 tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg aagctgatca
5881 acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag tccgacggct
5941 tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt aaagaggaca
6001 tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt gccaatctgg
6061 ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg gacgagctcg
6121 tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc agagagaacc
6181 agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc gaagagggca
6241 tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc cagctgcaga
6301 acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg gaccaggaac
6361 tggacatcaa ccggctgtcc gactacgatg tggacgctat cgtgcctcag agctttctga
6421 aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg ggcaagagcg
6481 acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg cagctgctga
6541 acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag agaggcggcc
6601 tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc cggcagatca
6661 caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac gagaatgaca
6721 agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc gatttccgga
6781 aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc cacgacgcct
6841 acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg gaaagcgagt
6901 tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag agcgagcagg
6961 aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac tttttcaaga
7021 ccgagattac cctggccaac ggcgagatcc ggaagcggcc tctgatcgag acaaacggcg
7081 aaaccgggga gatcgtgtgg gataagggcc gggattttgc caccgtgcgg aaagtgctga
7141 gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc ttcagcaaag
7201 agtctatcct gcccaagagg aacagcgata agctgatcgc cagaaagaag gactgggacc
7261 ctaagaagta cggcggcttc gacagcccca ccgtggccta ttctgtgctg gtggtggcca
7321 aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa agagctgctg gggatcacca
7381 tcatggaaag aagcagcttc gagaagaatc ccatcgactt tctggaagcc aagggctaca
7441 aagaagtgaa aaaggacctg atcatcaagc tgcctaagta ctccctgttc gagctggaaa
7501 acggccggaa gagaatgctg gcctctgccg gcgaactgca gaagggaaac gaactggccc
7561 tgccctccaa atatgtgaac ttcctgtacc tggccagcca ctatgagaag ctgaagggct
7621 cccccgagga taatgagcag aaacagctgt ttgtggaaca gcacaagcac tacctggacg
7681 agatcatcga gcagatcagc gagttctcca agagagtgat cctggccgac gctaatctgg
7741 acaaagtgct gtccgcctac aacaagcacc gggataagcc catcagagag caggccgaga
7801 atatcatcca cctgtttacc ctgaccaatc tgggagcccc tgccgccttc aagtactttg
7861 acaccaccat cgaccggaag aggtacacca gcaccaaaga ggtgctggac gccaccctga
7921 tccaccagag catcaccggc ctgtacgaga cacggatcga cctgtctcag ctgggaggtg
7981 actccggcgg aagctctggt ggcagcaagc ggaccgccga cggctctgaa ttcgagagcc
8041 ctaagaagaa aagaaaggtg gctactaact tcagcctgct gaagcaggct ggagacgtgg
8101 aggagaaccc tggacctagc gagctgatta aggagaacat gcacatgaag ctgtacatgg
8161 agggcaccgt ggacaaccat cacttcaagt gcacatccga gggcgaaggc aagccctacg
8221 agggcaccca gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg
8281 acatcctggc tactagcttc ctctacggca gcaagacctt catcaaccac acccagggca
8341 tccccgactt cttcaagcag tccttccctg agggcttcac atgggagaga gtcaccacat
8401 acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccaggac ggctgcctca
8461 tctacaacgt caagatcaga ggggtgaact tcacatccaa cggccctgtg atgcagaaga
8521 aaacactcgg ctgggaggcc ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag
8581 gcagaaacga catggccctg aagctcgtgg gcgggagcca tctgatcgca aacatcaaga
8641 ccacatatag atccaagaaa cccgctaaga acctcaagat gcctggcgtc tactatgtgg
8701 actacagact ggaaagaatc aaggaggcca acaacgagac ctacgtcgag cagcacgagg
8761 tggcagtggc cagatactgc gacctcccta gcaaactggg gcacaagctt aattagacgc
8821 gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
8881 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
8941 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
9001 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
9061 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
9121 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
9181 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
9241 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
9301 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
9361 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
9421 gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
9481 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
9541 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
9601 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
9661 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
9721 tagcagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
9781 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
9841 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
9901 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
9961 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
10021 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
10081 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
10141 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
10201 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
10261 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
10321 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
10381 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
10441 aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
10501 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
10561 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
10621 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
10681 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
10741 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
10801 gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca
10861 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag
10921 ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg
10981 accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg
11041 gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg
11101 gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc
11161 acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg
11221 cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag
11281 gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
11341 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
11401 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
11461 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
11521 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
11581 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
11641 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
11701 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
11761 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
11821 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
11881 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
11941 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
12001 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
12061 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
12121 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
12181 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
12241 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
12301 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
12361 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
12421 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
12481 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
12541 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
12601 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
12661 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
12721 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
12781 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
12841 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
12901 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
12961 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
13021 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
13081 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
13141 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
13201 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
13261 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
13321 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
13381 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
13441 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
13501 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
13561 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
13621 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
13681 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
//
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