pXlone_GRAB_HTR6_3xGGGGS_HaloTag

LOCUS      pXlone_GRAB_HTR6_3xGGGGS_HaloTag               8763 bp DNA     circular SYN 28-DEC-2023
DEFINITION  synthetic circular DNA
ACCESSION   VB200909-1152ryz
VERSION     .
KEYWORDS    VB200909-1152ryz.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 29, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8763
                     /organism="synthetic DNA construct"
     misc_feature    9..376
                     /label=TRE3GS promoter
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=TRE3GS promoter"
                     /note="/official_designation=TRE3GS promoter"
                     /note="/uuid=ef3841bf7e82447ab6010004abb7bfb1"
                     /note="/vntifkey=21"
     misc_feature    377..382
                     /label=KpnI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=KpnI"
                     /note="/official_designation=KpnI"
                     /note="/uuid=0ca3b9c82c704e30800d68808aa4b838"
                     /note="/vntifkey=21"
     misc_feature    389..394
                     /label=Kozak
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates translation 
                     initiation ofATG start codon downstream of the Kozak 
                     sequence."
                     /note="/full_name=Kozak"
                     /note="/name=Kozak"
                     /note="/official_designation=Kozak"
                     /note="/uuid=5f18b25a1234443494a618363f09f052"
                     /note="/vntifkey=21"
     misc_feature    395..2602
                     /label=GRAB_HTR6(new)
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=GRAB_HTR6(new)"
                     /note="/official_designation=GRAB_HTR6(new)"
                     /note="/uuid=c5d5fb56dd4b4aa899e37bd30c4230e7"
                     /note="/vntifkey=21"
     primer_bind     395..412
                     /label=引物 1
     misc_feature    2603..2650
                     /label=3xGGGGS
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Commonly used flexible linker 
                     forfusing two proteins."
                     /note="/full_name=3 tandem GGGGS"
                     /note="/name=3xGGGGS"
                     /note="/official_designation=3xGGGGS"
                     /note="/uuid=061d4a7627b844ccbc3750d338a8fad3"
                     /note="/vntifkey=21"
     misc_feature    2651..3544
                     /label=Halo tag
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=Halo tag"
                     /note="/official_designation=Halo tag"
                     /note="/uuid=c3d8b9ce6fc24cd0a89642a642a26fa0"
                     /note="/vntifkey=21"
     misc_feature    3545..3550
                     /label=SpeI
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=SpeI"
                     /note="/official_designation=SpeI"
                     /note="/uuid=fc8372cb4c1249e8a1abfca3c3762c5b"
                     /note="/vntifkey=21"
     misc_feature    3725..3859
                     /label=SV40 poly(A) signal
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=SV40 poly(A) signal"
                     /note="/official_designation=SV40 poly(A) signal"
                     /note="/uuid=db7a5a52bd3d4fac809cac839d0aafe5"
                     /note="/vntifkey=21"
     primer_bind     complement(3810..3839)
                     /label=SV40-R-SEQ
     misc_feature    complement(3861..4173)
                     /label=5' ITR
                     /label=nonstandard type:  ITR
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Recognized by PBase transposase; 
                     DNAflanked by piggyBac 5' ITR and 3' ITR can be transposed 
                     by PBase into TTAA sites."
                     /note="/description=piggyBac 5' inverted terminal repeat"
                     /note="/name=5' ITR"
                     /note="/official_designation=5' ITR"
                     /note="/uuid=23d6038d45c04788be6b660fdc405f37"
                     /note="/vntifkey=21"
     misc_feature    3861..3866
                     /label=EcoRV
                     /note="/__SeqFeature__=True"
                     /note="/__level__=1"
                     /note="/name=EcoRV"
                     /note="/official_designation=EcoRV"
                     /note="/uuid=52960f2c9920488b9b15415f353f3884"
                     /note="/vntifkey=21"
     misc_feature    4775..5635
                     /label=AmpR
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows E. coli to be resistant 
                     toampicillin."
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/name=AmpR"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=6a7994dc58024d80b36e4a85ad2e1d99"
                     /note="/vntifkey=21"
     misc_feature    5806..6394
                     /label=ori
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates plasmid replication 
                     in E.coli; regulates high-copy plasmid number (500-700)."
                     /note="/full_name=pUC origin of replication"
                     /note="/name=ori"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=b978ad4ff785460db448caf63b728237"
                     /note="/vntifkey=21"
     misc_feature    6818..7052
                     /label=3' ITR
                     /label=nonstandard type:  ITR
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Recognized by PBase transposase; 
                     DNAflanked by piggyBac 5' ITR and 3' ITR can be transposed 
                     by PBase into TTAA sites."
                     /note="/description=piggyBac 3' inverted terminal repeat"
                     /note="/name=3' ITR"
                     /note="/official_designation=3' ITR"
                     /note="/uuid=a9f9e08c756b4ff799b34ebdcdbd03a7"
                     /note="/vntifkey=21"
     misc_feature    complement(7060..7141)
                     /label=SV40 poly(A) signal
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=SV40 poly(A) signal"
                     /note="/official_designation=SV40 poly(A) signal"
                     /note="/uuid=98b1d2bc4a484ea09f93bf5f72628170"
                     /note="/vntifkey=21"
     primer_bind     complement(7092..7121)
                     /label=SV40-R-SEQ
     misc_feature    complement(7258..7656)
                     /label=BSD
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows cells to be resistant 
                     toblasticidin."
                     /note="/full_name=Blasticidin resistance gene"
                     /note="/name=BSD"
                     /note="/official_designation=Bsd"
                     /note="/uuid=679779db5a5941d0a0a88aa2fee4e5c4"
                     /note="/vntifkey=21"
     misc_feature    complement(7657..7722)
                     /label=P2A
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=P2A"
                     /note="/official_designation=P2A"
                     /note="/uuid=c8cabe774f3542e4b15d560bc36097d9"
                     /note="/vntifkey=21"
     misc_feature    complement(7723..8466)
                     /label=Tet-On 3G
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=It binds to TRE3G promoter to 
                     activategene transcription only in the presence of 
                     tetracycline or its analogs (e.g. doxycycline). It has 
                     higher sensitivity to the inducing drug compared to its 
                     predecessor, rtTA."
                     /note="/name=Tet-On 3G"
                     /note="/official_designation=Tet3G"
                     /note="/uuid=57b0b1d4a3734c518dcb447a93f70011"
                     /note="/vntifkey=21"
     misc_feature    complement(8467..8493)
                     /label=FLAG
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=FLAG"
                     /note="/official_designation=FLAG"
                     /note="/uuid=23ca82346bdd495ab492ca6d1ce7af9e"
                     /note="/vntifkey=21"
     misc_feature    complement(8494..8499)
                     /label=Kozak
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Facilitates translation 
                     initiation ofATG start codon downstream of the Kozak 
                     sequence."
                     /note="/full_name=Kozak"
                     /note="/name=Kozak"
                     /note="/official_designation=Kozak"
                     /note="/uuid=ba4f837cfff042e38e7561244b24cfa6"
                     /note="/vntifkey=21"
     misc_feature    complement(8519..8730)
                     /label=EF-1alpha core promoter
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/name=EF-1alpha core promoter"
                     /note="/official_designation=EF-1alpha core promoter"
                     /note="/uuid=7da7ee7458db409aaab468938100361e"
                     /note="/vntifkey=21"
ORIGIN
        1 atcacctcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc
       61 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt
      121 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa
      181 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc
      241 agtgatagag aacgtataag ctttgcttat gtaaaccagg gcgcctataa aagagtgctg
      301 attttttgag taaacttcaa ttccacaaca cttttgtctt ataccaactt tccgtaccac
      361 ttcctaccct cgtaaaggta ccacgcgtgc caccatggtg cccgagccgg gcccaactgc
      421 taacagcacc ccagcttggg gggctggtcc tccaagcgcc ccaggtggca gtggttgggt
      481 tgctgcagca ttgtgcgtgg ttatcgccct gacagctgcc gcaaacagcc tgctcattgc
      541 tcttatttgt acccaacccg cactccggaa tacgtctaat tttttcctgg tctcactgtt
      601 cacgagcgac cttatggtcg ggctcgtagt catgcctcct gctatgttga acgccctgta
      661 tgggagatgg gtcctggcaa ggggtctttg ccttctgtgg accgcatttg acgtcatgtg
      721 ctgtagtgcc agcatcttga acctctgttt gattagtctg gatagatatc tgctgattct
      781 gagcccactt agatacaagc tgcggatgac tccgctccgg gccctcgctt tggtgctggg
      841 cgcctggtct ctggcagcac tggcttcatt cttgcctttg ctgctgggct ggcacgaatt
      901 ggggcacgca aggccgccag ttccaggcca gtgtaggctg cttgcctccc tgcccttcgt
      961 gttggtggct tccggcctca cattctttct gcctagtggt gccatctgct tcacttactg
     1021 ccggatactt ctggcagcta gaaaacaagc cgttcaggtt gcctccctca ccaccggcat
     1081 ggcaagccag cggggtccgg acgccgtcgc cgcgccgccg gggggcaccg agcgcaggcc
     1141 caacggtctg ggccccgagc gcagcgcggg cccggggggc gcagaggccg aaccgctgcc
     1201 cacccagctc aacggcgccc ctggcgagcc cgcgccggcc gggccgcgcg acaccgacgc
     1261 gctggacctg gaggagggag gaaacgtcta tatcaaggcc gacaagcaga agaacggcat
     1321 caaggcgaac ttccacatcc gccacaacat cgaggacggc ggcgtgcagc tcgcctacca
     1381 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     1441 gagcgtgcag tccaaacttt cgaaagaccc caacgagaag cgcgatcaca tggtcctgct
     1501 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agggcggtac
     1561 cggagggagc atggtgagaa agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     1621 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gtgagggcga
     1681 tgccaccgag ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     1741 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     1801 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctaca tccaggagcg
     1861 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     1921 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     1981 cctggggcac aagctggagt acaacaccgg agcagcagca cgctggcact caagaaaggc
     2041 gctgaaagct tcattgagct tgggcatctt gctgggcatg ttcttcgtaa catggctccc
     2101 attttttgtg gccaatattg tccaggcggt gtgcgactgc atctcaccag gcctgttcga
     2161 tgtgctcact tggctggggt attgcaattc cactatgaac cccatcatat atcccctgtt
     2221 catgagagat ttcaagcgag cactcggaag gtttctgcca tgcccacggt gcccacgcga
     2281 aaggcaagca agtctggcct ccccctcact ccggacctcc cattcaggac ccagaccagg
     2341 actttcactc cagcaagttc tgcccctgcc acttcctcca gattcagact ctgactccga
     2401 tgcgggttca ggtggctcta gcggactccg gcttacggcc cagcttttgc ttcccgggga
     2461 agcaactcag gacccaccat tgcctactcg ggctgccgcc gccgtcaatt tcttcaacat
     2521 tgatcctgca gagccagaac tcagaccgca tcctctgggt ataccaacca ttaggctagc
     2581 gcccctctcc ctcccccccc cctctggtgg cggaggctcg ggcggaggtg ggtcgggtgg
     2641 cggcggatca atggccgaga tcggcaccgg cttccccttc gacccccact acgtggaggt
     2701 gctgggcgag cgcatgcact acgttgacgt gggcccccgc gacggcaccc ccgtgctgtt
     2761 cctgcacggc aaccccacca gcagctacgt gtggcgcaac atcatccccc acgtggcccc
     2821 cacccaccgc actatcgccc ccgacctgat cggcatgggc aagagcgaca agcccgacct
     2881 gggctacttc ttcgacgacc acgtgcgctt catggacgcc ttcatcgagg ccctgggcct
     2941 ggaggaggtg gtgctggtga tccacgactg gggcagcgcc ctgggcttcc actgggccaa
     3001 gcgcaacccc gagcgcgtga agggcatcgc cttcatggag ttcatccgcc ccatccccac
     3061 ctgggacgag tggcccgagt tcgcccgcga gaccttccag gccttccgca ccaccgacgt
     3121 gggccgcaag ctgatcatcg accagaacgt gttcatcgag ggcaccctgc ccatgggcgt
     3181 ggtgcgcccc ctgaccgagg tggagatgga ccactaccgc gagcccttcc tgaaccccgt
     3241 ggaccgcgag cccctgtggc gcttccccaa cgagctgccc atcgccggcg agcccgccaa
     3301 catcgtggcc ctggtggagg agtacatgga ctggctgcac cagagccccg tgcccaagct
     3361 gctgttctgg ggcacccccg gcgtgctgat cccccccgcc gaggccgccc gcctggccaa
     3421 gagcctgccc aacgttaagg ccgtggacat cggccccggc ctgaacctgc tccaggagga
     3481 caaccccgac ctgatcggca gcgagatcgc ccgctggctg agcaccctgg agatcagcgg
     3541 ctgaactagt agaccacctc ccctgcgagc taagctggac agccaatgac gggtaagaga
     3601 gtgacatttt tcactaacct aagacaggag ggccgtcaga gctactgcct aatccaaaga
     3661 cgggtaaaag tgataaaaat gtatcactcc aacctaagac aggcgcagct tccgagggat
     3721 ttgagatcca gacatgataa gatacattga tgagtttgga caaaccaaaa ctagaatgca
     3781 gtgaaaaaaa tgccttattt gtgaaatttg tgatgctatt gccttatttg taaccattat
     3841 aagctgcaat aaacaagttt gatatctata acaagaaaat atatatataa taagttatca
     3901 cgtaagtaga acatgaaata acaatataat tatcgtatga gttaaatctt aaaagtcacg
     3961 taaaagataa tcatgcgtca ttttgactca cgcggtcgtt atagttcaaa atcagtgaca
     4021 cttaccgcat tgacaagcac gcctcacggg agctccaagc ggcgactgag atgtcctaaa
     4081 tgcacagcga cggattcgcg ctatttagaa agagagagca atatttcaag aatgcatgcg
     4141 tcaattttac gcagactatc tttctagggt taagaattca ctggccgtcg ttttacaacg
     4201 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     4261 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     4321 cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     4381 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
     4441 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     4501 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
     4561 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     4621 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     4681 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     4741 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     4801 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     4861 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     4921 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     4981 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     5041 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     5101 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     5161 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     5221 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     5281 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     5341 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     5401 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     5461 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     5521 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     5581 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     5641 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     5701 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     5761 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     5821 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     5881 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     5941 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6001 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6061 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6121 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6181 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6241 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6301 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     6361 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     6421 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     6481 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     6541 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     6601 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     6661 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     6721 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     6781 aggaaacagc tatgaccatg attacgccaa ggtcgactta accctagaaa gataatcata
     6841 ttgtgacgta cgttaaagat aatcatgcgt aaaattgacg catgtgtttt atcggtctgt
     6901 atatcgaggt ttatttatta atttgaatag atattaagtt ttattatatt tacacttaca
     6961 tactaataat aaattcaaca aacaatttat ttatgtttat ttatttatta aaaaaaaaca
     7021 aaaactcaaa atttcttcta taaagtaaca aaacttttag cagtgaaaaa aatgctttat
     7081 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     7141 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     7201 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcctc tggagattta
     7261 gccctcccac acataaccag agggcagcaa ttcacgaatc ccaactgccg tcggctgtcc
     7321 atcactgtcc ttcactatgg ctttgatccc aggatgcaga tcgagaagca cctgtcggca
     7381 ccgtccgcag gggctcaaga tgcccctgtt ctcatttccg atcgcgacga tacaagtcag
     7441 gttgccagct gccgcagcag cagcagtgcc cagcaccacg agttctgcac aaggtccccc
     7501 agtaaaatga tatacattga caccagtgaa gatgcggccg tcgctagaga gagctgcgct
     7561 ggcgacgctg tagtcttcag agatggggat gctgttgatt gtagccgttg ctctttcaat
     7621 gagggtggat tcttcttgag acaaaggctt ggccatgggg ccggggttct cctccacgtc
     7681 gccggcctgc ttcagcaggc tgaagttggt ggcgccgctg cccccgggga gcatgtcaag
     7741 gtcaaaatcg tcaagagcgt cagcaggcag catatcaagg tcaaagtcgt caagggcatc
     7801 ggctgggagc atgtctaagt caaaatcgtc aagggcgtcg gtcggcccgc cgctttcgca
     7861 ctttagctgt ttctccaggc cacatatgat tagttccagg ccgaaaagga aggcaggttc
     7921 ggctccctgc cggtcgaaca gctcaattgc ttgtttcaga agtgggggca tagaatcggt
     7981 ggtaggtgtc tctctttcct cttttgctac ttgatgctcc tgttcctcca atacgcagcc
     8041 cagtgtaaag tggcccacgg cggacagagc gtacagtgcg ttctccaggg agaagccttg
     8101 ctgacacagg aacgcgagct gattttccag ggtttcgtac tgtttctctg ttgggcgggt
     8161 gccgagatgc actttagccc cgtcgcgatg tgagaggaga gcacagcggt atgacttggc
     8221 gttgttccgc agaaagtctt gccatgactc gccttccagg gggcaggagt gggtatgatg
     8281 cctgtccagc atctcgattg gcagggcatc gagcagggcc cgcttgttct tcacgtgcca
     8341 gtacagggta ggctgctcaa ctcccagctt ttgagcgagt ttccttgtcg tcaggccttc
     8401 gataccgact ccattgagta attccagagc agagtttatg actttgctct tgtccagtct
     8461 agacatctta tcgtcatcgt ctttgtaatc catggtggcg gatcccgcgt cacgacacct
     8521 gtgttctggc ggcaaacccg ttgcgaaaaa gaacgttcac ggcgactact gcacttatat
     8581 acggttctcc cccaccctcg ggaaaaaggc ggagccagta cacgacatca ctttcccagt
     8641 ttaccccgcg ccaccttctc taggcaccgg ttcaattgcc gacccctccc cccaacttct
     8701 cggggactgt gggcgatgtg cgctctgccc actgacgggc accggagcca ctcgagtgga
     8761 att
//