| 质粒名称: | mcherry p62 delta UBA silent |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 187984 |
| 质粒用途: | mcherry p62 lacking UBA domain (aa389-434) but it has the silent mutations to be resistant to sip62. Internal reference: SMC572 |
| 存储实验室: | Sascha Martens |
|
载体骨架基本信息 |
|
| 载体名称: | pmCherry-C1 Vector |
| 载体出品公司: | Clontech #632524 |
| 空载体大小: | 4722 bp |
| 完整质粒大小: | 5707 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 卡那霉素，50μg/mL |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | p62 |
| 物种来源: | H. sapiens (human) |
| 基因突变: | Lacking UBA domain (aa389-434) but it has the silent mutations to be resistant to sip62 #5 |
| GenBank编号 | 23636 NM_153719 |
| 标签/融合蛋白: | mCherry (N terminal on insert) |
| 克隆方法: | 未知 |
| 5’测序引物 | AAATGGCGTCGCTCACCGTGAAGGCCT |
| 3’测序引物 | ggTCACAACGGCGGGGGATGCTTTGGC |
|
文献引用方法 |
|
| 材料和方法部分: | mcherry p62 delta UBA silent was a gift from Sascha Martens (Addgene plasmid # 187984 ; http://n2t.net/addgene:187984 ; RRID:Addgene_187984) |
| 参考文献部分: | Oligomerization of p62 allows for selection of ubiquitinated cargo and isolation membrane during selective autophagy. Wurzer B, Zaffagnini G, Fracchiolla D, Turco E, Abert C, Romanov J, Martens S. Elife. 2015 Sep 28;4:e08941. doi: 10.7554/eLife.08941. 10.7554/eLife.08941 PubMed 26413874 |
mcherry p62 delta UBA silent
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
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mcherry p62 delta UBA silent载体质粒基本信息
mcherry p62 delta UBA silent质粒图谱载体图谱和mcherry p62 delta UBA silent载体序列质粒序列多克隆位点信息
mcherry p62 delta UBA silent质粒载体简介
mcherry p62 delta UBA silent质粒序列载体序列
LOCUS mcherry p62 delta UBA silent 5875 bp DNA circular SYN 04-SEP-2026
DEFINITION mcherry p62 lacking UBA domain (aa389-434) but it has the silent
mutations to be resistant to sip62. Internal reference: SMC572.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5875)
AUTHORS Wurzer B, Zaffagnini G, Fracchiolla D, Turco E, Abert C, Romanov J,
Martens S
TITLE Oligomerization of p62 allows for selection of ubiquitinated cargo
and isolation membrane during selective autophagy.
JOURNAL Elife. 2015 Sep 28;4:e08941. doi: 10.7554/eLife.08941.
PUBMED 26413874
REFERENCE 2 (bases 1 to 5875)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 4, 2026 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Elife.";
date: "2015-09-28"; volume: "4:e08941. doi"; pages: "
10.7554/eLife.08941"
COMMENT Sequence Label: mcherry p62 delta UBA silent
FEATURES Location/Qualifiers
source 1..5875
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 83..386
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 387..590
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 541..561
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 629..638
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 635..1342
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
primer_bind complement(780..798)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind 1048..1067
/label=mCherry-F
/note="mCherry, forward primer"
polyA_signal 2685..2806
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2722..2741)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2776..2795
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(2813..3268)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2950..2971)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 3162..3181
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 3295..3399
/gene="bla"
/label=AmpR promoter
promoter 3401..3758
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
enhancer 3401..3592
/label=SV40 enhancer
/note="enhancer for the SV40 early promoter (Herr, 1993)"
primer_bind complement(3424..3444)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 3609..3744
/label=SV40 ori
/note="SV40 origin of replication"
CDS 3793..4587
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
CDS 3793..4587
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3847..3866)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4457..4476
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(4775..4794)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 4819..4866
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 5195..5783
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5684..5703
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 taaccgtatt accgccatgc attagttatt aatagtaatc aattacgggg tcattagttc
61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga
601 accgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggaggataa
661 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
721 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
781 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
841 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
901 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
961 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
1021 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
1081 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
1141 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
1201 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
1261 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
1321 cggcatggac gagctgtaca agtccggact cagatctcga gctcaaatgg cgtcgctcac
1381 cgtgaaggcc taccttctgg gcaaggagga cgcggcgcgc gagattcgcc gcttcagctt
1441 ctgctgcagc cccgagcctg aggcggaagc cgaggctgcg gcgggtccgg gaccctgcga
1501 gcggctgctg agccgggtgg ccgccctgtt ccccgcgctg cggcctggcg gcttccaggc
1561 gcactaccgc gatgaggacg gggacttggt tgccttttcc agtgacgagg aattgacaat
1621 ggccatgtcc tacgtgaagg atgacatctt ccgaatctac attaaagaga aaaaagagtg
1681 ccggcgggac caccgcccac cgtgtgctca ggaggcgccc cgcaacatgg tgcaccccaa
1741 tgtgatctgc gatggctgca atgggcctgt ggtaggaacc cgctacaagt gcagcgtctg
1801 cccagactac gacttgtgta gcgtctgcga gggaaagggc ttgcaccggg ggcacaccaa
1861 gctcgcattc cccagcccct tcgggcacct gtctgagggc ttctcgcaca gccgctggct
1921 ccggaaggtg aaacacggac acttcgggtg gccaggatgg gaaatgggtc caccaggaaa
1981 ctggagccca cgtcctcctc gtgcagggga ggcccgccct ggccccacgg cagaatcagc
2041 ttctggtcca tcggaggatc cgagtgtgaa tttcctgaag aacgttgggg agagtgtggc
2101 agctgccctt agccctctgg gcattgaagt tgatatcgat gtggagcacg gagggaaaag
2161 aagccgcctg acccccgtct ctccagagag ttccagcaca gaggagaaga gcagctcaca
2221 gccaagcagc tgctgctctg accccagcaa gccgggtggg aatgttgagg gcgccacgca
2281 gtctctggcg gagcagatga ggaagatcgc cttggagtcc gaggggcgcc ctgaggagca
2341 aatggaatcc gacaactgtt caggaggaga tgatgactgg acccatctgt cttcaaaaga
2401 agtggacccg tctacaggtg aactccagtc cctacagatg ccagaatccg aagggccaag
2461 ctctctggac ccctcccagg agggacccac agggctgaag gaagctgcct tgtacccaca
2521 tctcccgcca aagcatcccc cgccgttgtg acccgggatc caccggatct agataactga
2581 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
2641 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
2701 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
2761 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta
2821 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
2881 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
2941 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
3001 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
3061 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
3121 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
3181 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
3241 gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga
3301 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
3361 ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc
3421 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
3481 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
3541 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
3601 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
3661 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
3721 agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga
3781 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
3841 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
3901 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
3961 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
4021 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
4081 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
4141 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
4201 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
4261 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
4321 agcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
4381 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
4441 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
4501 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
4561 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
4621 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
4681 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
4741 tggagttctt cgcccaccct agggggaggc taactgaaac acggaaggag acaataccgg
4801 aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggt gttgggtcgt
4861 ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcgatacc ccaccgagac
4921 cccattgggg ccaatacgcc cgcgtttctt ccttttcccc accccacccc ccaagttcgg
4981 gtgaaggccc agggctcgca gccaacgtcg gggcggcagg ccctgccata gcctcaggtt
5041 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
5101 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
5161 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
5221 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
5281 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
5341 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
5401 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
5461 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
5521 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
5581 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
5641 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
5701 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
5761 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
5821 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtgga
//
