| 质粒名称: | pAAVLINK-EF1a-HA-5'-TNRC6B |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001563 |
| 产品用途: | 5' AAVLINK plasmid for TNRC6B expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | TNRC6B |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-TNRC6B
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-TNRC6B载体质粒基本信息
pAAVLINK-EF1a-HA-5'-TNRC6B质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-TNRC6B载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-TNRC6B质粒载体简介
pAAVLINK-EF1a-HA-5'-TNRC6B质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-TNRC6B 6686 bp DNA circular SYN 17-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6686)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 18, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6686
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 187..398
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 411..679
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 505..510
/label=SDS
CDS 716..742
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 749..3826
/codon_start=1
/label=TNRC6B
/translation="REKEQEREEQLMEDKKRKKEDKKKKEATQKVTEQKTKVPEVTKPS
LSQPTAASPIGSSPSPPVNGGNNAKRVAVPNGQPPSAARYMPREVPPRFRCQQDHKVLL
KRGQPPPPSCMLLGGGAGPPPCTAPGANPNNAQVTGALLQSESGTAPDSTLGGAAASNY
ANSTWGSGASSNNGTSPNPIHIWDKVIVDGSDMEEWPCIASKDTESSSENTTDNNSASN
PGSEKSTLPGSTTSNKGKGSQCQSASSGNECNLGVWKSDPKAKSVQSSNSTTENNNGLG
NWRNVSGQDRIGPGSGFSNFNPNSNPSAWPALVQEGTSRKGALETDNSNSSAQVSTVGQ
TSREQQSKMENAGVNFVVSGREQAQIHNTDGPKNGNTNSLNLSSPNPMENKGMPFGMGL
GNTSRSTDAPSQSTGDRKTGSVGSWGAARGPSGTDTVSGQSNSGNNGNNGKEREDSWKG
ASVQKSTGSKNDSWDNNNRSTGGSWNFGPQDSNDNKWGEGNKMTSGVSQGEWKQPTGSD
ELKIGEWSGPNQPNSSTGAWDNQKGHPLPENQGNAQAPCWGRSSSSTGSEVGGQSTGSN
HKAGSSDSHNSGRRSYRPTHPDCQAVLQTLLSRTDLDPRVLSNTGWGQTQIKQDTVWDI
EEVPRPEGKSDKGTEGWESAATQTKNSGGWGDAPSQSNQMKSGWGELSASTEWKDPKNT
GGWNDYKNNNSSNWGGGRPDEKTPSSWNENPSKDQGWGGGRQPNQGWSSGKNGWGEEVD
QTKNSNWESSASKPVSGWGEGGQNEIGTWGNGGNASLASKGGWEDCKRSPAWNETGRQP
NSWNKQHQQQQPPQQPPPPQPEASGSWGGPPPPPPGNVRPSNSSWSSGPQPATPKDEEP
SGWEEPSPQSISRKMDIDDGTSAWGDPNSYNYKNVNLWDKNSQGGPAPREPNLPTPMTS
KSASVWSKSTPPAPDNGTSAWGEPNESSPGWGEMDDTGASTTGWGNTPANAPNAMKPNS
KSMQDGWGESDGPVTGARHPSWEEEEDGGVWNTTGSQ"
misc_feature 1115..1120
/label=SDS
misc_feature 1263..1268
/label=SDS
misc_feature 2830..2835
/label=SDS
misc_feature 2875..2880
/label=SDS
misc_feature 3067..3072
/label=SDS
misc_feature 3786..3791
/label=SDS
misc_feature 3789..3794
/label=SDS
intron 3827..3867
/label=Splicing Donor
misc_feature 3871..3904
/label=lox2272-JT1571
repeat_region 3949..4089
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3949..4078
/label=AAV2 ITR
rep_origin 4164..4619
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4901..5005
/gene="bla"
/label=AmpR promoter
CDS 5006..5866
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6037..6625
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
721 ttatgacgta cctgactatg ctggatctag agagaaggag caagaaaggg aagaacagtt
781 aatggaagac aagaaaagga agaaagagga taaaaagaaa aaagaagcca ctcagaaggt
841 cacggaacaa aaaaccaaag tgcccgaagt gacgaaacca agtttaagcc aaccaacggc
901 cgccagccca attggcagct ctccatcgcc accagtcaat ggtggcaaca atgccaaaag
961 ggtggcagtg ccgaacggac aaccgccaag cgccgcccgc tacatgcctc gggaggtgcc
1021 gccgcgattc cgttgccagc aggaccacaa agtgttacta aaacgtgggc agccccctcc
1081 accgtcctgc atgctccttg ggggtggggc agggcctcct ccctgcacag cacctggagc
1141 aaacccaaac aacgcacaag tgacaggagc gctgctgcag agtgagagtg ggactgcgcc
1201 agactcaacc cttggaggtg ctgctgcttc aaattatgca aattccactt ggggctcggg
1261 agcctcctcc aacaacggca cctcccccaa cccaattcac atctgggaca aggtgattgt
1321 agacgggtct gacatggaag agtggccttg tattgccagc aaagacactg aatcttcttc
1381 cgaaaacacc accgataaca acagtgcctc gaaccctggc tctgagaaga gcactctgcc
1441 aggaagcacc actagtaaca aaggaaaagg gagccagtgc cagtctgcaa gttctgggaa
1501 cgaatgtaat cttggggtct ggaaatctga ccctaaggct aaatctgttc aatcttccaa
1561 ctctactaca gagaacaaca atggactagg aaattggagg aatgtgagtg gtcaggatag
1621 aattggacct ggctctggct tcagcaactt taacccaaat agcaacccat ctgcctggcc
1681 agcactggtc caagaaggaa cttctaggaa aggggcattg gaaacagata atagtaattc
1741 cagtgcacag gttagcacag taggtcagac atccagggaa cagcagtcaa agatggaaaa
1801 tgcgggtgtt aattttgttg tctctggcag agaacaggct caaattcata acactgatgg
1861 accaaaaaat ggaaacacta actccttgaa cttaagttca ccaaacccca tggagaataa
1921 gggaatgccc tttggaatgg gcttggggaa cacctccagg agcactgatg ccccttcaca
1981 aagcactgga gatcgaaaga ctgggagtgt tggatcttgg ggtgcagcta gggggccttc
2041 tggaactgac acagtctctg gacaaagcaa ttctggaaac aatgggaaca atggaaaaga
2101 gagagaggac tcctggaaag gagcttctgt tcagaaatca actgggtcaa aaaatgactc
2161 ttgggacaac aataacaggt ctacgggtgg gtcctggaac tttggccccc aggactctaa
2221 tgacaacaaa tggggtgaag ggaacaaaat gacatctggg gtctctcagg gagaatggaa
2281 acagccgact gggtctgatg agttgaaaat tggagaatgg agtggtccaa accaaccaaa
2341 ttctagcact ggagcatggg acaatcaaaa gggccacccc ctccctgaaa accaaggcaa
2401 tgcccaggct ccctgttggg gaagatcttc cagctccaca ggaagtgaag ttggaggtca
2461 aagcactgga agcaaccaca aagcaggaag tagtgacagt cataactctg gccgtcggtc
2521 gtacaggccc acacatcctg attgtcaggc tgtcttgcag actcttttga gccgaactga
2581 tttggacccc agggtgctct caaacactgg ctggggccaa actcaaatta agcaggacac
2641 agtgtgggac attgaagagg tgccaaggcc tgaggggaaa tctgacaaag gaactgaggg
2701 gtgggagagc gctgccacac agaccaagaa ctcagggggc tggggagatg cacccagcca
2761 aagcaatcaa atgaagtctg gatgggggga gctctcagcc tctacagagt ggaaagaccc
2821 caagaacaca ggaggctgga atgactacaa gaacaacaac tcttccaact ggggaggagg
2881 acgacctgat gaaaagacac cttcctcttg gaatgagaat cccagcaagg atcaggggtg
2941 gggaggtgga cgccagccca atcaaggatg gtcttctgga aagaatggtt ggggggagga
3001 agtcgatcag acaaaaaaca gcaattggga aagttctgca agtaaacctg tgtctgggtg
3061 gggtgaagga gggcagaatg aaatcgggac ttggggtaat ggtggcaatg caagcctagc
3121 ttcaaaaggt gggtgggagg attgcaaaag atccccagca tggaatgaga cgggccgaca
3181 gcccaattcc tggaataaac aacaccaaca gcagcagccc ccacagcagc cgccgccacc
3241 acaaccagag gcttctggtt cgtggggagg cccaccccca ccacctccag gcaacgttcg
3301 accttccaat tccagctgga gcagcgggcc acagcctgca acacctaagg atgaggaacc
3361 cagtggttgg gaagagccat ccccacagtc aattagtcgg aaaatggaca ttgatgatgg
3421 cacttcagca tggggagacc ctaacagtta taactacaag aatgtgaatc tgtgggataa
3481 gaattcccaa gggggcccag cacctcgaga accaaacctg cccaccccaa tgaccagtaa
3541 atcggcatca gtctggagca aaagcacacc acctgctcca gataatggta cttccgcttg
3601 gggtgagcca aatgaaagca gtcctgggtg gggcgagatg gatgatacag gagcatcgac
3661 cacaggctgg gggaacacgc ccgccaacgc tcccaatgcc atgaagccta attccaaatc
3721 tatgcaagac ggctgggggg agagtgacgg gccagtcaca ggagctcgcc atcccagctg
3781 ggaagaggag gaggatggag gagtctggaa caccactggc tctcaggtaa gtatcaaggt
3841 tacaagacag gtttaaggag accaataagc tattattcgt ataggatact ttatacgaag
3901 ttattaagtt atggagaccg gtgtggcacg tgcggaccga gcggccgcag gaacccctag
3961 tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa
4021 aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct
4081 gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
4141 gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
4201 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc
4261 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
4321 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt
4381 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
4441 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaactctat
4501 ctcgggctat tcttttgatt tataagggat tttgccgatt tcggtctatt ggttaaaaaa
4561 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt
4621 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
4681 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
4741 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
4801 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
4861 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
4921 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
4981 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
5041 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
5101 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
5161 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
5221 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
5281 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
5341 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
5401 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
5461 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
5521 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
5581 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
5641 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
5701 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
5761 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
5821 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
5881 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
5941 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
6001 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
6061 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
6121 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
6181 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
6241 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
6301 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
6361 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
6421 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
6481 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
6541 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
6601 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
6661 cttttgctgg ccttttgctc acatgt
//
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