| 质粒名称: |
pAAVLINK-EF1a-HA-5'-PRR12
|
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001428 |
| 产品用途: | 5' AAVLINK plasmid for PRR12 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: |
PRR12
|
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINK-EF1a-HA-5'-PRR12载体质粒基本信息
pAAVLINK-EF1a-HA-5'-PRR12质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-PRR12载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-PRR12质粒载体简介
pAAVLINK-EF1a-HA-5'-PRR12质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-PRR12 7259 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7259)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 7, 2026 from SnapGene 6.0.2
https:///www.biofeng.com
FEATURES Location/Qualifiers
source 1..7259
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..141
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..141
/label=AAV2 ITR
promoter 169..711
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 198..409
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 422..690
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 516..521
/label=SDS
CDS 727..753
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 760..4399
/codon_start=1
/label=PRR12
/translation="DRNYPSAGFGDPLGAGAGWSYERSAKASLVYGSSRTSHPETDILH
RQAYAAPHPLQSYATNHHPAGLSGLFDTGLHHAGSAGPDASVMNLISALESRGPQPGPS
ASSLLSQFRSPSWQTAMHTPGPTELFISGALPGSSTFPSSSALSAYQHPASFGSRPFPV
PSSLSLQDPPFSPPANGLLSPHDVLHLKPSQAPTVPSSLGFERLAGGGVLGPAGLGPAQ
TPPYRPGPPDPPPPPRHLPTQFNLLASSSAAAAAAEQSSPQLYNFSGAAPGPPPPERAL
PRQDTVIKHYQRPASAQPPPPPPPAHALQHYLSCGGSYPSMGHRANLACSPLGGGEPSP
GAGEPSKAGPSGATAGASGRATGPEAAGGGGAGGGGGGYRPIIQSPGYKTGKGGYGAAA
GGATRPPPPRSTATPKCQSLGGPAAAYATGKASGAGGAGGQAYSPGQPQGLLGPQAYGQ
GFGGGQAQDLSKAPSYSGGPPQPPSGPPPPGLATCQSYSPDQLQGQLYGVQGEPYPGPA
AHSQGLPTASPSLSYSTGHSPALSGHGGGWGPSSLGGGGEASPSHIIRPLQSPPATGRP
PGVGSPGAPGKYLSSVLASAPFLAPPGAGSYAAGAGGYKGKGDGSELLAGPGGPPAERT
EDEEFLIQHLLQAPSPPRTSGADGLVGEDGAADASKGLGGSGGAGGPPGTPYELAKEDP
QRYHLQSVIRTSASLDEGATAALELGLGRLKEKKKGPERGGETPEGLATSVVHYGAGAK
ELGAFLQKSPPPPPPTAQSTQPTPHGLLLEAGGPDLPLVLPPPPPQLLPSVLSHAPSPS
PSASKVGVHLLEPATRDGAPQPPPPPPPPPPPMPLQLEAHLRSHGLEPAAPSPRLRPEE
SLDPPGAMQELLGALEPLPPAPGDTGVGPPNSEGKDPAGAYRSPSPQGTKAPRFVPLTS
ICFPDSLLQDEERSFFPTMEEMFGGGAADDYGKAGPPEDEGDPKAGAGPPPGPPAYDPY
GPYCPGRASGAGPETPGLGLDPNKPPELPSTVNAEPLGLIQSGPHQAAPPPPPPPPPPP
APASEPKGGLTSPIFCSTKPKKLLKTSSFHLLRRRDPPFQTPKKLYAQEYEFEADEDKA
DVPADIRLNPRRLPDLVSSCRSRPALSPLGDIDFCPPNPGPDGPRRRGRKPTKAKRDGP
PRPRGRPRIRPLEVPTTAGPASASTPTDGAKKPRGRGRGRGRKAEEA"
misc_feature 1073..1078
/label=SDS
misc_feature 1465..1470
/label=SDS
misc_feature 2215..2220
/label=SDS
misc_feature 2218..2223
/label=SDS
misc_feature 2647..2652
/label=SDS
misc_feature 2710..2715
/label=SDS
misc_feature 3096..3101
/label=SDS
misc_feature 3222..3227
/label=SDS
misc_feature 3983..3988
/label=SDS
misc_feature 4391..4396
/label=SDS
intron 4400..4440
/label=Splicing Donor
misc_feature 4444..4477
/label=lox2272-JT1571
repeat_region 4522..4662
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4522..4651
/label=AAV2 ITR
rep_origin 4737..5192
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5474..5578
/gene="bla"
/label=AmpR promoter
CDS 5579..6439
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6610..7198
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
721 accatgtacc cttatgacgt acctgactat gctggatctg acaggaacta ccccagcgcc
781 ggcttcgggg acccgctcgg cgccggggcg ggatggagtt acgagaggtc agcgaaagct
841 agcttggttt atggcagctc caggacctcg caccccgaga cggacatctt acaccgccag
901 gcctatgcgg ccccccaccc actgcaaagc tatgccacca accaccaccc ggcaggcctc
961 tctggactct tcgacactgg cctccaccac gcgggctcag cagggcccga cgcctccgtc
1021 atgaacctca tctcggccct ggaatcccgg ggcccccagc ctggcccctc cgcctcctct
1081 ctcctctccc agttccgcag tccttcctgg caaacagcca tgcacacgcc aggccccacg
1141 gagctcttca tctcgggtgc cctgccgggt tccagcacct ttccgtcctc atctgccctg
1201 tcggcttacc aacacccggc ttccttcggc agccgcccct tcccagtgcc ctcgtccctc
1261 agcctccagg accccccatt cagccctcca gctaacgggc tcctgtcccc tcatgacgtg
1321 ctgcacctga agccctcgca ggcacccacg gtgccctctt cactgggctt cgagcgcctg
1381 gcagggggcg gtgtcttggg gccagctggt ctcggtccag cccagacccc cccttaccgc
1441 cctggccccc cagacccacc accacctcct cgccacctcc caactcagtt caacctgctg
1501 gcttcctctt ccgctgccgc cgccgctgcc gagcagtcct ccccacagct ctataacttc
1561 tcgggtgctg ccccgggccc accgccacct gagcgggccc tgccacgcca ggacacggtc
1621 atcaagcact accagcggcc agccagtgcc cagcccccac cacccccgcc accagcccat
1681 gcgctccagc actatctgag ctgtggaggc agctacccct ccatgggcca ccgggccaac
1741 ctggcctgca gccccctggg tggtggggag ccctccccgg gtgctgggga gcctagcaag
1801 gctggtccca gcggagccac ggctggggca tctggccggg ccacgggccc tgaggcagca
1861 gggggcggtg gggctggggg tggtggtgga ggttaccgcc ccatcattca gtcgcctggg
1921 tacaagacgg gcaaaggtgg ttatggagca gctgccgggg gtgccaccag gcccccccca
1981 ccccgttcga ccgccacccc caaatgtcag agcctgggtg ggccagcagc cgcctatgcc
2041 actgggaagg cctctggggc tggaggggca gggggccagg cttattcccc cggtcagcct
2101 caagggcttc tgggacccca ggcctacggg caagggtttg gaggggggca ggcacaggac
2161 ttgagcaaag cccccagcta ctcagggggc cccccacagc cccccagcgg cccccctcct
2221 cctggcctgg ccacatgtca gagctactcc ccggaccagc tgcaggggca gctgtatggg
2281 gtgcagggcg agccataccc agggccagcc gcccactccc aggggctgcc cacagccagc
2341 ccctcgctca gctacagtac cggccattcc ccagcgctct cgggccatgg gggtggctgg
2401 ggacccagct ccctgggagg cggcggtgag gccagcccat ctcacatcat tcgtccgctc
2461 cagtcaccgc ctgccaccgg ccgtccacct ggagtcggct ctccaggagc ccctggcaaa
2521 tacctgagct cagtcttggc ctcagcgcct ttcctggcac ctccgggagc tggcagctat
2581 gcagctggag caggtggcta caagggcaag ggggatggct cggagctgct ggcgggccca
2641 ggtgggcctc ctgcggagcg cacagaggat gaggagttcc ttatccagca cctcttgcag
2701 gcgcccagcc ctcctcggac ctcaggggcg gacggcttgg tgggcgagga cggggcagca
2761 gatgcctcta agggacttgg ggggagtggc ggggccgggg gaccaccggg tacaccctac
2821 gagttggcca aggaagaccc ccagaggtac cacctgcaga gtgtcatccg caccagtgcc
2881 agcctggatg agggtgccac tgcggcactg gagctgggcc tggggaggct gaaggagaag
2941 aagaaagggc cagagcgggg tggcgagacc cccgaggggc tggccacctc tgttgtccac
3001 tacggggcag gcgccaagga gctgggggcc ttcttgcaaa agagccctcc gcccccacct
3061 cccacggccc agtctaccca gcccactccc catggcctcc ttctggaggc cgggggccct
3121 gacctcccac tggtgctgcc tccgcctccc ccccagctgc tcccctcggt cctcagccat
3181 gcccccagtc cctctcccag cgcctccaaa gtcggcgtcc acctccttga gccagccacc
3241 cgcgatgggg caccccagcc acctccaccg ccacccccgc ctccaccacc catgcccctg
3301 cagctcgagg cccacctccg cagccatggc ctggagcccg cggcccccag cccccgcctg
3361 cgacccgagg agagcctgga tccgccaggc gccatgcagg aattgctcgg ggctctggag
3421 ccgctgcccc cggcgcctgg ggatactggc gtaggcccgc caaactcgga gggcaaggat
3481 cccgcaggcg cctaccgcag ccccagcccg caaggcacca aggcgccgcg tttcgtgccg
3541 ctcacctcca tctgcttccc tgactccttg ctccaagacg aggagcgcag cttcttcccc
3601 accatggagg agatgttcgg tggaggggcc gcggacgact acggcaaggc cgggccacct
3661 gaggacgagg gggaccccaa ggctggcgct gggccacccc ccggcccccc tgcttatgat
3721 ccctatgggc cctactgtcc tggccgggcg tcgggagccg ggcccgagac accgggcctg
3781 ggcctggacc ccaacaaacc gcctgaactg ccctccacgg tcaacgccga gccgctgggc
3841 ctgatccaga gtggccccca ccaggcggcg ccaccacccc cgcctccgcc accgccgcct
3901 cccgcgccgg cctccgaacc caagggtggc ctcacctcgc ccatcttctg ctctaccaag
3961 ccaaagaagc tgctcaagac atcctccttc cacctgctgc ggcgccgcga cccacccttc
4021 cagaccccca agaagctgta cgcccaggag tacgagttcg aggcggacga ggacaaggcc
4081 gatgttcccg ccgacatccg cctcaacccc cggcgcttgc ctgacctggt ctccagctgc
4141 cgctcccgtc cggccctctc gccactgggg gacatcgact tctgcccacc caacccagga
4201 cccgatggcc cccggcgccg tggccgcaag cccacgaagg cgaaacgtga tgggccaccc
4261 cggccacggg ggaggccccg gatccgcccc ctggaggtcc cgaccactgc ggggcccgcc
4321 tcggcctcca cgcccaccga tggcgccaag aaaccccggg gccggggccg aggccggggt
4381 cgaaaggctg aggaggcagg taagtatcaa ggttacaaga caggtttaag gagaccaata
4441 agctattatt cgtataggat actttatacg aagttattaa gttatggaga ccggtgtggc
4501 acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc
4561 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc
4621 gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt
4681 ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc
4741 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
4801 acttgccagc gccttagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
4861 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
4921 tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc
4981 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
5041 cttgttccaa actggaacaa cactcaactc tatctcgggc tattcttttg atttataagg
5101 gattttgccg atttcggtct attggttaaa aaatgagctg atttaacaaa aatttaacgc
5161 gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc
5221 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
5281 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
5341 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
5401 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
5461 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
5521 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
5581 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
5641 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
5701 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
5761 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
5821 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
5881 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
5941 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
6001 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
6061 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
6121 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
6181 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
6241 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
6301 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
6361 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
6421 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
6481 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
6541 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
6601 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
6661 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
6721 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
6781 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
6841 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
6901 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
6961 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
7021 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
7081 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
7141 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
7201 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt
//
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pAAVLINK-EF1a-HA-5'-PRR12质粒载体应用举例
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