pAAVLINK-EF1a-HA-5'-KAT6A

LOCUS       pAAVLINK-EF1a-HA-5'-KAT6A                7280 bp DNA     circular SYN 18-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7280)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported May 20, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7280
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     promoter        187..398
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             411..679
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     misc_feature    505..510
                     /label=SDS
     CDS             716..742
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             749..4420
                     /codon_start=1
                     /label=KAT6A
                     /translation="MVKLANPLYTEWILEAIKKVKKQKQRPSEERICNAVSSSHGLDRK
                     TVLEQLELSVKDGTILKVSNKGLNSYKDPDNPGRIALPKPRNHGKLDNKQNVDWNKLIK
                     RAVEGLAESGGSTLKSIERFLKGQKDVSALFGGSAASGFHQQLRLAIKRAIGHGRLLKD
                     GPLYRLNTKATNVDGKESCESLSCLPPVSLLPHEKDKPVAEPIPICSFCLGTKEQNREK
                     KPEELISCADCGNSGHPSCLKFSPELTVRVKALRWQCIECKTCSSCRDQGKNADNMLFC
                     DSCDRGFHMECCDPPLTRMPKGMWICQICRPRKKGRKLLQKKAAQIKRRYTNPIGRPKN
                     RLKKQNTVSKGPFSKVRTGPGRGRKRKITLSSQSASSSSEEGYLERIDGLDFCRDSNVS
                     LKFNKKTKGLIDGLTKFFTPSPDGRKARGEVVDYSEQYRIRKRGNRKSSTSDWPTDNQD
                     GWDGKQENEERLFGSQEIMTEKDMELFRDIQEQALQKVGVTGPPDPQVRCPSVIEFGKY
                     EIHTWYSSPYPQEYSRLPKLYLCEFCLKYMKSRTILQQHMKKCGWFHPPANEIYRKNNI
                     SVFEVDGNVSTIYCQNLCLLAKLFLDHKTLYYDVEPFLFYVLTQNDVKGCHLVGYFSKE
                     KHCQQKYNVSCIMILPQYQRKGYGRFLIDFSYLLSKREGQAGSPEKPLSDLGRLSYMAY
                     WKSVILECLYHQNDKQISIKKLSKLTGICPQDITSTLHHLRMLDFRSDQFVIIRREKLI
                     QDHMAKLQLNLRPVDVDPECLRWTPVIVSNSVVSEEEEEEAEEGENEEPQCQERELEIS
                     VGKSVSHENKEQDSYSVESEKKPEVMAPVSSTRLSKQVLPHDSLPANSQPSRRGRWGRK
                     NRKTQERFGDKDSKLLLEETSSAPQEQYGECGEKSEATQEQYTESEEQLVASEEQPSQD
                     GKPDLPKRRLSEGVEPWRGQLKKSPEALKCRLTEGSERLPRRYSEGDRAVLRGFSESSE
                     EEEEPESPRSSSPPILTKPTLKRKKPFLHRRRRVRKRKHHNSSVVTETISETTEVLDEP
                     FEDSDSERPMPRLEPTFEIDEEEEEEDENELFPREYFRRLSSQDVLRCQSSSKRKSKDE
                     EEDEESDDADDTPILKPVSLLRKRDVKNSPLEPDTSTPLKKKKGWPKGKSRKPIHWKKR
                     PGRKPGFKLSREIMPVSTQACVIEPIVSIPKAGRKPKIQESEETVEPKEDMPLPEERK"
     misc_feature    2441..2446
                     /label=SDS
     misc_feature    3111..3116
                     /label=SDS
     misc_feature    3123..3128
                     /label=SDS
     misc_feature    3714..3719
                     /label=SDS
     misc_feature    3717..3722
                     /label=SDS
     misc_feature    3720..3725
                     /label=SDS
     misc_feature    3806..3811
                     /label=SDS
     misc_feature    3960..3965
                     /label=SDS
     intron          4421..4461
                     /label=Splicing Donor
     misc_feature    4465..4498
                     /label=lox2272-JT1571
     repeat_region   4543..4683
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4543..4672
                     /label=AAV2 ITR
     rep_origin      4758..5213
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5495..5599
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5600..6460
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6631..7219
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
      181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
      421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
      481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
      541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
      601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
      661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
      721 ttatgacgta cctgactatg ctggatctat ggtaaaactc gcaaacccgc tttatactga
      781 gtggattttg gaggccatca aaaaagtgaa aaagcagaaa cagcgtcctt cagaagaaag
      841 gatatgcaat gctgtgtctt catcccatgg cttggatcgt aaaactgttt tagaacaatt
      901 ggagttgagt gttaaagatg gaacaatttt aaaagtctca aataaaggac tcaattccta
      961 taaagatcct gataatcctg ggcgaatagc acttcctaag cctcggaacc atggaaaatt
     1021 ggataataaa caaaatgtgg attggaataa actgataaag cgggcagttg agggcttggc
     1081 agagtctggt ggctcaactt tgaaaagcat tgaacgtttt ttgaaaggtc agaaggatgt
     1141 gtctgcatta ttcggaggca gtgctgcctc tggctttcac cagcagttac gattggctat
     1201 caaacgtgcc attggccacg gcagactcct taaagatgga cctctttatc ggctcaacac
     1261 taaagcaacc aacgtggatg ggaaagagag ttgtgagtct ctttcctgtt tacctccagt
     1321 gtcccttctt ccacatgaaa aggataagcc ggttgctgaa ccaatcccca tctgtagttt
     1381 ctgtcttggt acaaaagaac aaaaccgaga aaagaagcca gaggaactca tctcctgtgc
     1441 cgactgtggc aacagtggcc atccatcctg tttaaagttt tcccctgaac taacggttcg
     1501 agtgaaggcc ttacggtggc agtgcatcga gtgtaaaaca tgcagctcct gtcgagatca
     1561 aggcaaaaat gcggataaca tgctcttttg tgattcatgt gaccgaggtt ttcacatgga
     1621 gtgttgtgat ccgccactca cccgtatgcc aaaaggcatg tggatatgtc aaatatgtcg
     1681 acctaggaaa aaaggacgaa aacttctaca aaagaaggca gcacagataa aacggcgcta
     1741 tactaatcca ataggacgtc caaaaaacag gttaaagaaa caaaacacgg tatcaaaagg
     1801 tcccttcagc aaagttcgaa ctggccctgg aaggggtagg aaacgaaaaa tcactctttc
     1861 cagccaatca gcatcatcat catcagaaga aggatattta gagcggatag atggcttgga
     1921 cttctgcaga gatagcaatg tctccttgaa gttcaacaag aaaaccaaag ggctcattga
     1981 tggccttacc aaatttttta ccccttcccc tgatgggcgg aaagctcggg gggaagtggt
     2041 ggactactct gagcaatatc gaatcagaaa gaggggcaac aggaaatcaa gcacttcaga
     2101 ttggcccaca gacaatcagg atggctggga tggcaaacaa gaaaatgagg agcgactttt
     2161 tgggagccag gaaatcatga ctgagaaaga tatggaatta tttcgtgata tccaagaaca
     2221 agcactgcag aaagttggag tgactggtcc ccctgatcca caagtccgct gtccctctgt
     2281 cattgagttt gggaagtatg aaattcacac ctggtactcc tccccatatc ctcaagaata
     2341 ctcaaggctg cccaaattgt atctttgtga attttgtcta aaatatatga aaagtagaac
     2401 tattctgcag cagcacatga agaaatgtgg ttggttccat cctcctgcca atgagattta
     2461 cagaaagaat aatatttctg tctttgaggt tgatgggaat gtgagtacca tttattgtca
     2521 aaacctgtgt cttttggcaa agttgtttct tgaccacaaa accctctatt acgatgtgga
     2581 gccatttctt ttttatgtac taacacagaa tgatgtcaag ggctgccacc ttgttggcta
     2641 cttttctaag gaaaagcact gccaacagaa gtacaatgtt tcctgtataa tgattcttcc
     2701 tcaataccag cgtaagggct atggcaggtt tctcatcgat ttcagttatt tgttatcaaa
     2761 gcgtgaaggc caagcagggt ctccagagaa accgttatct gatctgggtc gtctttccta
     2821 catggcatat tggaaaagtg taatattgga gtgcctttat caccaaaatg acaagcagat
     2881 cagcattaag aagttaagca agttgactgg aatctgccct caagacatca cttccacact
     2941 ccaccaccta cgaatgctgg acttccgtag tgaccaattt gtgattatcc gccgggaaaa
     3001 acttatccag gatcacatgg caaagcttca gctgaatttg cgacctgtag atgtagatcc
     3061 agaatgtttg cgctggactc cagtcatagt gtccaactct gtggtctcag aggaggaaga
     3121 agaggaggct gaggaaggag aaaacgaaga gccacagtgc caggaaagag aattagagat
     3181 cagtgtggga aagtctgtgt ctcatgagaa caaagaacaa gattcttatt cagtagaaag
     3241 tgaaaagaaa ccagaagtta tggctccagt cagttctaca cgtttgagca aacaagtcct
     3301 tcctcatgat agtcttcctg caaatagcca gccatctcgg aggggccgct gggggaggaa
     3361 gaacagaaaa acccaggaac gttttggtga taaagattct aaactgctct tggaagagac
     3421 gtcttcagct cctcaggaac aatatggaga atgtggggag aaatcagaag ccacccagga
     3481 acaatacact gaaagtgaag aacagctggt ggcttctgag gagcagccaa gccaggacgg
     3541 gaaacctgac cttcccaaga gaagactcag tgagggggtt gagccctggc gaggacagct
     3601 caagaaaagc cctgaggctc tgaagtgcag attaacagaa ggaagtgaga ggctgccccg
     3661 tcgctacagt gagggtgaca gggctgtcct caggggcttc agtgagagca gcgaggagga
     3721 ggaggagccg gaaagccctc ggtcaagctc gccaccaatt ctcacaaagc ccacgctgaa
     3781 gcgaaagaaa ccatttctcc accgaaggag gagagtccga aagcgcaaac accacaatag
     3841 cagtgtagtc acagaaacta tttctgagac cactgaagtg ttagatgaac cttttgaaga
     3901 ttctgactcc gagaggccaa tgccaagatt agaacccacg tttgagatcg atgaagaaga
     3961 ggaggaagag gatgaaaatg aacttttccc tagagaatac ttccgtcgtt tgtcttcgca
     4021 ggatgtactc aggtgtcagt cctcttctaa gaggaagtct aaagatgaag aagaagatga
     4081 agagtcagat gatgctgatg acactcctat cttaaagcca gtatctcttt tgcgaaaacg
     4141 tgatgtgaag aattctcctc ttgagccaga tacatccaca cctttgaaaa agaaaaaggg
     4201 atggcccaaa ggcaagagcc gcaaaccaat ccactggaag aaaagacctg gtcgaaaacc
     4261 aggatttaag ttgagtcggg aaatcatgcc agtttctact caagcatgcg tcattgagcc
     4321 catcgtttcc attcctaaag ctggacgtaa acccaagatc caggagagtg aagaaactgt
     4381 tgagccaaaa gaagacatgc ccctacccga ggagaggaag gtaagtatca aggttacaag
     4441 acaggtttaa ggagaccaat aagctattat tcgtatagga tactttatac gaagttatta
     4501 agttatggag accggtgtgg cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg
     4561 agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg
     4621 cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc
     4681 aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
     4741 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     4801 tacgcgcagc gtgaccgcta cacttgccag cgccttagcg cccgctcctt tcgctttctt
     4861 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     4921 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
     4981 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     5041 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaact ctatctcggg
     5101 ctattctttt gatttataag ggattttgcc gatttcggtc tattggttaa aaaatgagct
     5161 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg
     5221 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     5281 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     5341 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     5401 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     5461 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     5521 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     5581 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     5641 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     5701 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     5761 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     5821 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     5881 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     5941 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     6001 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     6061 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     6121 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     6181 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     6241 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     6301 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     6361 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     6421 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     6481 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     6541 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     6601 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     6661 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     6721 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     6781 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     6841 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     6901 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     6961 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     7021 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     7081 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     7141 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     7201 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     7261 ctggcctttt gctcacatgt
//