| 质粒名称: | pAAVLINK-EF1a-HA-5'-GLI3 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001596 |
| 产品用途: | 5' AAVLINK plasmid for GLI3 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | GLI3 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-5'-GLI3
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-5'-GLI3载体质粒基本信息
pAAVLINK-EF1a-HA-5'-GLI3质粒图谱载体图谱和pAAVLINK-EF1a-HA-5'-GLI3载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-5'-GLI3质粒载体简介
pAAVLINK-EF1a-HA-5'-GLI3质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-5'-GLI3 6938 bp DNA circular SYN 22-JUL-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6938)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 19, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6938
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..141
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..141
/label=AAV2 ITR
promoter 169..711
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 198..409
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 422..690
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 516..521
/label=SDS
CDS 727..753
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
misc_feature 760..4078
/label=GLI3
misc_feature 1129..1134
/label=SDS
misc_feature 1156..1161
/label=SDS
misc_feature 2144..2149
/label=SDS
misc_feature 3570..3575
/label=SDS
misc_feature 3688..3693
/label=SDS
misc_feature 3766..3771
/label=SDS
intron 4079..4119
/label=Splicing Donor
misc_feature 4123..4156
/label=lox2272-JT1571
repeat_region 4201..4341
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4201..4330
/label=AAV2 ITR
rep_origin 4416..4871
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5153..5257
/gene="bla"
/label=AmpR promoter
CDS 5258..6118
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6289..6877
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
121 actccatcac taggggttcc tgcggccgca cgcgtgtcga ctctagagga tctgcgatcg
181 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
241 aggggtcggc aattgaacgg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
301 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
361 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagc tgaagcttcg
421 aggggctcgc atctctcctt cacgcgcccg ccgccctacc tgaggccgcc atccacgccg
481 gttgagtcgc gttctgccgc ctcccgcctg tggtgcctcc tgaactgcgt ccgccgtcta
541 ggtaagttta aagctcaggt cgagaccggg cctttgtccg gcgctccctt ggagcctacc
601 tagactcagc cggctctcca cgctttgcct gaccctgctt gctcaactct acgtctttgt
661 ttcgttttct gttctgcgcc gttacagatc caagctgtga ccggcgccta ccgtacggcc
721 accatgtacc cttatgacgt acctgactat gctggatcta tggaggccca gtcccacagc
781 tccacgacca ctgaaaagaa aaaagttgag aattccatag tgaagtgctc cactcgaaca
841 gatgtgagcg agaaagccgt tgcctccagc accacttcta atgaggatga aagtcctgga
901 cagacttatc acagagagag aagaaacgca atcactatgc agccacagaa tgtccagggg
961 ctcagcaaag tcagtgagga accttcaaca tcgagtgacg agagggcctc attgatcaag
1021 aaagagatcc atgggtccct gccacacgtg gcggagccct ctgtgccgta ccgcgggacg
1081 gtgtttgcca tggaccccag gaatggttac atggagcccc actaccaccc tcctcatctt
1141 ttccctgcct tccatcctcc tgtaccaatt gatgccagac atcatgaggg ccgttaccat
1201 tacgatccat ctccgattcc tccattgcat atgacttccg ccttatctag tagccctacg
1261 tatccggacc tgcccttcat taggatctcc ccacaccgga accccactgc tgcttccgag
1321 tctcccttca gccctccaca tccctacatt aatccctaca tggactatat ccgctccttg
1381 cacagcagcc catcgctctc catgatctca gcaacccgtg ggctgagccc tacagatgcg
1441 ccccatgcag gagtcagccc agcagaatac tatcatcaga tggccctgct aactggccag
1501 cgcagcccct atgcagacat tattccctca gctgccaccg ccggcacggg ggccatccac
1561 atggaatatc ttcatgctat ggatagcacc agattctcca gccccaggct gtcagccagg
1621 ccgagccgaa aacgtacact gtccatatca ccactctccg atcatagctt tgaccttcag
1681 accatgataa ggacgtctcc caactccttg gtcacgattc tcaataattc ccgtagcagc
1741 tcttcagcaa gtggctccta tggtcactta tctgcaagtg caatcagccc tgccttgagc
1801 ttcacctact cttccgcgcc cgtctctctc cacatgcatc agcagatcct aagccgacaa
1861 cagagcttag gttcagcctt tggacacagc cctccactca tccaccctgc cccaactttt
1921 ccaacacaga ggcctattcc agggatccct acggttctga accccgtcca ggtcagctcc
1981 ggcccttctg agtcctcaca gaacaagccc acgagtgagt ctgcagtgag cagcactggt
2041 gacccgatgc acaacaagag gtccaagatc aaacccgatg aagacctccc cagcccaggg
2101 gctcgggggc agcaggaaca gcccgaagga acaacccttg tcaaggagga aggggacaaa
2161 gatgaaagca aacaggagcc tgaagtcatc tatgagacaa actgccactg ggaaggctgc
2221 gcgagggagt tcgacaccca agagcagctt gtgcaccata taaataacga ccatattcat
2281 ggagagaaga aggagttcgt gtgcaggtgg ctggactgct caagagagca gaaacccttc
2341 aaagcccagt atatgttggt agtgcatatg agaagacaca cgggcgagaa gcctcacaaa
2401 tgcacttttg aaggttgcac aaaggcctac tcgagactag aaaacttgaa aacacacttg
2461 agatctcaca ctggagagaa accatacgtc tgtgagcacg aaggttgcaa caaggctttc
2521 tcaaatgcct ctgatcgcgc caaacaccaa aacagaacgc attccaatga gaaaccatat
2581 gtgtgcaaaa tcccaggctg cactaagcgt tacacagacc caagctccct ccggaaacat
2641 gtgaagacag tgcatggccc agaggctcat gtcaccaaga agcagcgagg ggacatccat
2701 cctcggccgc cacccccgag agattccggc agccattcac agtccaggtc gcctggccga
2761 ccgactcagg gagcccttgg tgagcagcag gacctcagca acactacctc aaagcgggaa
2821 gaatgcctcc aggtgaaaac cgtcaaggca gagaagccaa tgacatctca gccaagccct
2881 ggtggtcagt cttcatgcag cagccaacag tcccccatca gcaactattc caacagtggg
2941 ctcgagcttc ctctgaccga tggaggtagt ataggagacc tcagtgccat cgatgaaacc
3001 ccaatcatgg actcaaccat ttccactgca accacagccc ttgctttgca agccaggaga
3061 aacccggcag ggaccaaatg gatggagcac gtaaaactag aaaggctaaa acaagtgaat
3121 ggaatgtttc cgcgactgaa ccccattcta ccccctaaag cccctgcggt ctctcctctc
3181 ataggaaatg gcacacagtc caacaacacc tgcagcttgg gtgggcccat gacgcttctc
3241 ccgggcagaa gcgacctctc tggggtggac gtcactatgc tgaacatgct caacagaagg
3301 gacagcagcg ccagcaccat cagctcggcc tacctgagca gccgccgctc ctcagggatc
3361 tcgccctgct tctccagccg ccgctccagc gaggcgtcac aggccgaggg ccggccgcag
3421 aacgtgagcg tggccgactc ctacgacccc atctccaccg acgcctcgcg ccgctccagc
3481 gaagccagcc agagcgacgg cctgcccagc ctgctcagcc tcacgcccgc ccagcagtac
3541 cgcctcaagg ccaagtacgc ggctgccaca ggagggccgc cgccgacgcc cctgcccaac
3601 atggagagga tgagcctgaa gacgcgcctg gcgctgctcg gggatgccct cgagcctggc
3661 gtggccctgc ctccagttca tgccccgagg aggtgcagcg acgggggagc ccacggctac
3721 gggcggcgcc acctgcagcc gcacgatgcg ccgggccacg gcgtgaggag ggccagcgac
3781 ccggtgcgga caggctccga gggcctggcc ctgcctcgtg tgccgcgctt cagcagcctc
3841 agcagctgca accccccggc gatggccacg tccgcggaga agcgcagtct cgtgcttcag
3901 aattacacgc ggcccgaggg cggccagtcc cgaaacttcc actcgtcccc ctgtcctccc
3961 agcatcaccg agaacgtcac cctggagtcc ctgaccatgg acgctgatgc caacctgaac
4021 gatgaggatt tcctgccgga cgacgtggtg cagtatttaa attcccagaa ccaagcaggt
4081 aagtatcaag gttacaagac aggtttaagg agaccaataa gctattattc gtataggata
4141 ctttatacga agttattaag ttatggagac cggtgtggca cgtgcggacc gagcggccgc
4201 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg
4261 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc
4321 gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg
4381 gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag
4441 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
4501 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
4561 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
4621 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
4681 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
4741 actcaaccct atctcgggct attcttttga tttataaggg attttgccga tttcggccta
4801 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
4861 gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
4921 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
4981 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
5041 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
5101 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
5161 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
5221 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
5281 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
5341 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
5401 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
5461 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
5521 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
5581 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
5641 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
5701 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
5761 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
5821 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
5881 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
5941 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
6001 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
6061 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
6121 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
6181 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
6241 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
6301 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
6361 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
6421 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
6481 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
6541 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
6601 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
6661 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
6721 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
6781 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
6841 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
6901 acggttcctg gccttttgct ggccttttgc tcacatgt
//
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