出品公司: | -- |
---|---|
载体名称: | pCS22+ |
质粒类型: | 克隆载体;哺乳动物表达载体;广宿主载体;体外表达载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | simian CMV IE94、lac、T3、SP6 |
载体大小: | 4046 bp |
5' 测序引物及序列: | SP6 fwd(MCS1)、T3 fwd(MCS2) |
3' 测序引物及序列: | EBV rev(MCS1)、SV40 polyA rev(MCS2) |
载体标签: | 无 |
载体抗性: | 氨苄青霉素 |
克隆菌株: | DH10B |
表达细胞: | 原核细胞、哺乳动物、非洲爪蟾、鸟类、斑马鱼。 |
备注: | pCS22+载体能够在多种细胞中进行高水平的瞬表达。pCS22+载体同时也能进行体外转录和翻译。 |
产品目录号: | -- |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
pCS22+
价格:2500元
联系方式:I47-825O-882O
买家导航
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS Exported 4046 bp ds-DNA circular SYN 31-JUL-2016 DEFINITION Vector that allows high-level transient expression in vertebrate cells as well as in vitro transcription/translation. KEYWORDS pCS22+ SOURCE Biofeng Lab. ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4046) AUTHORS I.M.A.G.E. Consortium COMMENT This file is created by Vector NTI http://www.biofeng.com/ COMMENT ORIGDB|GenBank COMMENT VNTAUTHORNAME|biofeng.com| COMMENT VNTNAME|pCS22+| FEATURES Location/Qualifiers source 1..4046 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 34..52 /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" misc_feature 69..101 /note="MCS1" promoter complement(103..121) /note="T3 promoter" /note="promoter for bacteriophage T3 RNA polymerase" polyA_signal 128..262 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" misc_feature 330..362 /note="MCS2" protein_bind 405..421 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(429..459) /note="lac promoter" /note="promoter for the E. coli lac operon" rep_origin complement(783..1371) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1542..2402) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(2403..2507) /gene="bla" /note="AmpR promoter" rep_origin complement(2533..2988) /direction=LEFT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 3062..4046 /note="CMV IE94 promoter" /note="enhancer/promoter region of simian cytomegalovirus major immediate early transcription unit IE94" ORIGIN 1 cgccattctg cctggggacg tcggagcaag ctttaatacg actcactata gggcaataca 61 agctacttgt ttaaactctg cagggatccg aattcctcga gccctttagt gagggttaat 121 ttacgtagat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat 181 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat 241 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca 301 gggggaggtg tgggaggttt tttaattcgc ggccgcggcg ccaatgcatt gggcccggta 361 ccgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 421 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 481 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 541 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 601 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 661 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 721 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 781 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 841 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 901 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 961 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 1021 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 1081 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 1141 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 1201 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt 1261 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 1321 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 1381 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 1441 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 1501 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 1561 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 1621 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 1681 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 1741 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 1801 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 1861 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 1921 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 1981 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 2041 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 2101 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 2161 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 2221 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 2281 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 2341 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 2401 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 2461 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 2521 aaagtgccac ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt 2581 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag 2641 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga 2701 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg 2761 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc 2821 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg 2881 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc 2941 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat 3001 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagt 3061 cgaccatagc caattcaata tggcgtatat ggactcatgc caattcaata tggtggatct 3121 ggacctgtgc caattcaata tggcgtatat ggactcgtgc caattcaata tggtggatct 3181 ggaccccagc caattcaata tggcggactt ggcaccatgc caattcaata tggcggactt 3241 ggcactgtgc caactgggga ggggtctact tggcacggtg ccaagtttga ggaggggtct 3301 tggccctgtg ccaagtccgc catattgaat tggcatggtg ccaataatgg cggccatatt 3361 ggctatatgc caggatcaat atataggcaa tatccaatat ggccctatgc caatatggct 3421 attggccagg ttcaatacta tgtattggcc ctatgccata tagtattcca tatatgggtt 3481 ttcctattga cgtagatagc ccctcccaat gggcggtccc atataccata tatggggctt 3541 cctaataccg cccatagcca ctcccccatt gacgtcaatg gtctctatat atggtctttc 3601 ctattgacgt catatgggcg gtcctattga cgtatatggc gcctccccca ttgacgtcaa 3661 ttacggtaaa tggcccgcct ggctcaatgc ccattgacgt caataggacc acccaccatt 3721 gacgtcaatg ggatggctca ttgcccattc atatccgttc tcacgccccc tattgacgtc 3781 aatgacggta aatggcccac ttggcagtac atcaatatct attaatagta acttggcaag 3841 tacattacta ttggaaggac gccagggtac attggcagta ctcccattga cgtcaatggc 3901 ggtaaatggc ccgcgatggc tgccaagtac atccccattg acgtcaatgg ggaggggcaa 3961 tgacgcaaat gggcgttcca ttgacgtaaa tgggcggtag gcgtgcctaa tgggaggtct 4021 atataagcaa tgctcgttta gggaac //