出品公司: | -- |
---|---|
载体名称: | pCS2(-) |
质粒类型: | 克隆载体;哺乳动物表达载体;广宿主载体;体外表达载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | simian CMV IE94、lac、T3、SP6 |
载体大小: | 4095 bp |
5' 测序引物及序列: | SP6 fwd(MCS1)、T3 fwd(MCS2) |
3' 测序引物及序列: | EBV rev(MCS1)、SV40 polyA rev(MCS2) |
载体标签: | 无 |
载体抗性: | 氨苄青霉素 |
克隆菌株: | DH10B |
表达细胞: | 原核细胞、哺乳动物、非洲爪蟾、鸟类、斑马鱼。 |
备注: | pCS2(-)载体能够在多种细胞中进行高水平的瞬表达。pCS2(-)载体同时也能进行体外转录和翻译。 |
产品目录号: | -- |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
pCS2-
价格:2500元
联系方式:I47-825O-882O
买家导航
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCS2- 4095 bp DNA SYN DEFINITION pCS2- ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI http://www.biofeng.com/ COMMENT ORIGDB|GenBank COMMENT VNTAUTHORNAME|biofeng.com| COMMENT VNTNAME|pCS2(-)| FEATURES Location/Qualifiers source 1..4095 /organism="pCS2-" /mol_type="other DNA" misc_feature 35..52 /label="Sp6_primer" terminator complement(154..273) /label="SV40_PA_terminator" misc_feature complement(168..187) /label="EBV_rev_primer" promoter complement(390..409) /label="T3_promoter" promoter complement(426..444) /label="M13_reverse_primer" misc_feature complement(443..465) /label="M13_pUC_rev_primer" promoter complement(479..508) /label="lac_promoter" gene complement(1591..2451) /label="Ampicillin" /gene="Ampicillin" CDS complement(1591..2451) /label="ORF frame 1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" promoter complement(2493..2521) /label="AmpR_promoter" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" rep_origin 2600..2906 /label="f1_origin" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" misc_feature 4013..4054 /label="CMV_fwd_primer" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" ORIGIN 1 CGCCATTCTG CCTGGGGACG TCGGAGCAAG CTTGATTTAG GTGACACTAT AGAATACAAG 61 CTACTTGTTC TTTTTGCAGG ATCCCATCGA TTCGAATTCA AGGCCTCTCG AGCCTCTAGA 121 ACTATAGTGA GTCGTATTAC GTAGATCCAG ACATGATAAG ATACATTGAT GAGTTTGGAC 181 AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG 241 CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT 301 TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA ATTCGCGGCC GCGGCGCCAA 361 TGCATTGGGC CCGGTACCCA GCTTTTGTTC CCTTTAGTGA GGGTTAATTG CGCGCTTGGC 421 GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA 481 CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC 541 ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA 601 TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC 661 CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC 721 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 781 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 841 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 901 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 961 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 1021 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 1081 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 1141 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 1201 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 1261 CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 1321 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 1381 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 1441 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 1501 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 1561 AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT 1621 CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC 1681 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG 1741 CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG 1801 TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT 1861 AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT 1921 GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT 1981 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT 2041 CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT 2101 TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT 2161 CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC 2221 CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA 2281 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA 2341 CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA 2401 AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT 2461 TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA 2521 ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC 2581 TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC 2641 CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC 2701 CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT 2761 TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG 2821 GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG 2881 TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT 2941 ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT 3001 TAACGCGAAT TTTAACAAAA TATTAACGCT TACAATTTCC ATTCGCCATT CAGGCTGCGC 3061 AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGTC GACCATAGCC 3121 AATTCAATAT GGCGTATATG GACTCATGCC AATTCAATAT GGTGGATCTG GACCTGTGCC 3181 AATTCAATAT GGCGTATATG GACTCGTGCC AATTCAATAT GGTGGATCTG GACCCCAGCC 3241 AATTCAATAT GGCGGACTTG GCACCATGCC AATTCAATAT GGCGGACTTG GCACTGTGCC 3301 AACTGGGGAG GGGTCTACTT GGCACGGTGC CAAGTTTGAG GAGGGGTCTT GGCCCTGTGC 3361 CAAGTCCGCC ATATTGAATT GGCATGGTGC CAATAATGGC GGCCATATTG GCTATATGCC 3421 AGGATCAATA TATAGGCAAT ATCCAATATG GCCCTATGCC AATATGGCTA TTGGCCAGGT 3481 TCAATACTAT GTATTGGCCC TATGCCATAT AGTATTCCAT ATATGGGTTT TCCTATTGAC 3541 GTAGATAGCC CCTCCCAATG GGCGGTCCCA TATACCATAT ATGGGGCTTC CTAATACCGC 3601 CCATAGCCAC TCCCCCATTG ACGTCAATGG TCTCTATATA TGGTCTTTCC TATTGACGTC 3661 ATATGGGCGG TCCTATTGAC GTATATGGCG CCTCCCCCAT TGACGTCAAT TACGGTAAAT 3721 GGCCCGCCTG GCTCAATGCC CATTGACGTC AATAGGACCA CCCACCATTG ACGTCAATGG 3781 GATGGCTCAT TGCCCATTCA TATCCGTTCT CACGCCCCCT ATTGACGTCA ATGACGGTAA 3841 ATGGCCCACT TGGCAGTACA TCAATATCTA TTAATAGTAA CTTGGCAAGT ACATTACTAT 3901 TGGAAGGACG CCAGGGTACA TTGGCAGTAC TCCCATTGAC GTCAATGGCG GTAAATGGCC 3961 CGCGATGGCT GCCAAGTACA TCCCCATTGA CGTCAATGGG GAGGGGCAAT GACGCAAATG 4021 GGCGTTCCAT TGACGTAAAT GGGCGGTAGG CGTGCCTAAT GGGAGGTCTA TATAAGCAAT 4081 GCTCGTTTAG GGAAC //