出品公司: | -- |
---|---|
载体名称: | pCS108 |
质粒类型: | 克隆载体;哺乳动物表达载体;广宿主载体;体外表达载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | lac、T3、SP6、CMV IE94 |
载体大小: | 4149 bp |
5' 测序引物及序列: | SP6 fwd(MCS1)、T3 fwd(MCS2) |
3' 测序引物及序列: | EBV rev(MCS1)、SV40 polyA rev(MCS2) |
载体标签: | 无 |
载体抗性: | 氨苄青霉素 |
克隆菌株: | DH10B |
表达细胞: | 原核细胞、哺乳动物、非洲爪蟾、鸟类、斑马鱼。 |
备注: | pCS108载体能够在多种细胞中进行高水平的瞬表达。pCS108载体同时也能进行体外转录和翻译。 |
产品目录号: | -- |
稳定性: | 瞬表达 |
组成型/诱导型: | -- |
病毒/非病毒: | 非病毒 |
pCS108
价格:2500元
联系方式:I47-825O-882O
买家导航
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS Exported 4149 bp ds-DNA circular SYN 29-JUL-2016 DEFINITION Vector that allows high-level transient expression in vertebrate cells as well as in vitro transcription/translation. ACCESSION DQ649433 VERSION . KEYWORDS pCS108 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4149) AUTHORS . TITLE Direct Submission FEATURES Location/Qualifiers source 1..4149 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 35..53 /note="SP6 promoter" /note="promoter for bacteriophage SP6 RNA polymerase" misc_feature 79..158 /note="MCS1" polyA_signal 190..324 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" misc_feature 393..428 /note="MCS2" promoter complement(441..459) /note="T3 promoter" /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(480..496) /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" misc_feature complement(482..484) /note="ATG" protein_bind 504..520 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(528..558) /note="lac promoter" /note="promoter for the E. coli lac operon" rep_origin complement(882..1470) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1641..2501) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(2502..2606) /gene="bla" /note="AmpR promoter" rep_origin complement(2632..3087) /direction=LEFT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 3165..4149 /note="CMV IE94 promoter" /note="enhancer/promoter region of simian cytomegalovirus major immediate early transcription unit IE94" ORIGIN 1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag 61 ctacttgttc tttttgcagg atcccatcga ttcgaattcg tcgacaggcc aatctggccg 121 cggccgcaag gcctgatatc tctagaccgc ggctcgagcc tctcgcccta tagtgagtcg 181 tattacgtag atccagacat gataagatac attgatgagt ttggacaaac cacaactaga 241 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc 301 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt 361 cagggggagg tgtgggaggt tttttaattc gcggcgcgcc gcggcgccaa tgcattgggc 421 ccggtaccca gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg 481 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 541 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 601 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 661 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 721 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 781 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 841 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 901 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 961 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 1021 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 1081 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 1141 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 1201 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 1261 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 1321 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 1381 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 1441 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 1501 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 1561 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 1621 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 1681 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 1741 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 1801 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 1861 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 1921 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 1981 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 2041 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 2101 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 2161 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 2221 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 2281 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 2341 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 2401 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 2461 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 2521 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 2581 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa 2641 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc 2701 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga 2761 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag 2821 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt 2881 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta 2941 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag 3001 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg 3061 cgcttaatgc gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg 3121 aagggcgatc ggtgcgggcc tcttcgctat tacgccagtc gatcgaccat agccaattca 3181 atatggcgta tatggactca tgccaattca atatggtgga tctggacctg tgccaattca 3241 atatggcgta tatggactcg tgccaattca atatggtgga tctggacccc agccaattca 3301 atatggcgga cttggcacca tgccaattca atatggcgga cttggcactg tgccaactgg 3361 ggaggggtct acttggcacg gtgccaagtt tgaggagggg tcttggccct gtgccaagtc 3421 cgccatattg aattggcatg gtgccaataa tggcggccat attggctata tgccaggatc 3481 aatatatagg caatatccaa tatggcccta tgccaatatg gctattggcc aggttcaata 3541 ctatgtattg gccctatgcc atatagtatt ccatatatgg gttttcctat tgacgtagat 3601 agcccctccc aatgggcggt cccatatacc atatatgggg cttcctaata ccgcccatag 3661 ccactccccc attgacgtca atggtctcta tatatggtct ttcctattga cgtcatatgg 3721 gcggtcctat tgacgtatat ggcgcctccc ccattgacgt caattacggt aaatggcccg 3781 cctggctcaa tgcccattga cgtcaatagg accacccacc attgacgtca atgggatggc 3841 tcattgccca ttcatatccg ttctcacgcc ccctattgac gtcaatgacg gtaaatggcc 3901 cacttggcag tacatcaata tctattaata gtaacttggc aagtacatta ctattggaag 3961 tacgccaggg tacattggca gtactcccat tgacgtcaat ggcggtaaat ggcccgcgat 4021 ggctgccaag tacatcccca ttgacgtcaa tggggagggg caatgacgca aatgggcgtt 4081 ccattgacgt aaatgggcgg taggcgtgcc taatgggagg tctatataag caatgctcgt 4141 ttagggaac //