出品公司: | -- |
---|---|
载体名称: | pCS111 |
质粒类型: | 克隆载体;哺乳动物表达载体;广宿主载体;体外表达载体 |
高拷贝/低拷贝: | 高拷贝 |
克隆方法: | 限制性内切酶,多克隆位点 |
启动子: | simian CMV IE94、lac、T3、SP6 |
载体大小: | 4146 bp |
5' 测序引物及序列: | SP6 fwd(MCS1)、T3 fwd(MCS2) |
3' 测序引物及序列: | EBV rev(MCS1)、SV40 polyA rev(MCS2) |
载体标签: | 无 |
载体抗性: | 氨苄青霉素 |
克隆菌株: | DH10B |
表达细胞: | 原核细胞、哺乳动物、非洲爪蟾、鸟类、斑马鱼。 |
备注: | pCS111载体能够在多种细胞中进行高水平的瞬表达。pCS111载体同时也能进行体外转录和翻译。 |
产品目录号: | -- |
稳定性: | 稳表达 |
组成型/诱导型: | 组成型 |
病毒/非病毒: | 非病毒 |
pCS111
价格:2500元
联系方式:I47-825O-882O
买家导航
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS Exported 4146 bp ds-DNA circular SYN 29-JUL-2016 DEFINITION Vector that allows high-level transient expression in vertebrate cells and in vitro transcription/translation. ACCESSION DQ649434 KEYWORDS pCS111 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4146) AUTHORS Biofeng Lab. COMMENT This file is created by Vector NTI http://www.biofeng.com/ COMMENT ORIGDB|GenBank COMMENT VNTAUTHORNAME|biofeng.com| FEATURES Location/Qualifiers source 1..4146 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 35..53 /note="SP6 promoter" /note="promoter for bacteriophage SP6 RNA polymerase" misc_feature 79..156 /note="MCS1" polyA_signal 187..321 /note="SV40 poly(A) signal" /note="SV40 polyadenylation signal" misc_feature 390..425 /note="MCS2" promoter complement(438..456) /note="T3 promoter" /note="promoter for bacteriophage T3 RNA polymerase" misc_feature complement(479..481) /note="ATG" protein_bind 501..517 /bound_moiety="lac repressor encoded by lacI" /note="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(525..555) /note="lac promoter" /note="promoter for the E. coli lac operon" rep_origin complement(879..1467) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1638..2498) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(2499..2603) /gene="bla" /note="AmpR promoter" rep_origin complement(2629..3084) /direction=LEFT /note="f1 ori" /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 3162..4146 /note="CMV IE94 promoter" /note="enhancer/promoter region of simian cytomegalovirus major immediate early transcription unit IE94" ORIGIN 1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag 61 ctacttgttc tttttgcagg atcccatcga ttcgaattcg tcccgggaat ctggccgcgg 121 ccgcaaggcc tgatatctct agaccgcggc tcgagcctct cgccctatag tgagtcgtat 181 tacgtagatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg 241 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt 301 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag 361 ggggaggtgt gggaggtttt ttaattcgcg gcgcgccgcg gcgccaatgc attgggcccg 421 gtacccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca 481 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 541 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 601 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 661 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 721 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 781 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 841 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 901 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 961 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 1021 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 1081 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 1141 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 1201 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 1261 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 1321 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 1381 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 1441 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 1501 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 1561 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 1621 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 1681 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 1741 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 1801 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 1861 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 1921 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 1981 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 2041 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 2101 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 2161 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 2221 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 2281 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 2341 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 2401 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 2461 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 2521 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 2581 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg 2641 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat 2701 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg 2761 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc 2821 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt 2881 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag 2941 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg 3001 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc 3061 ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag 3121 ggcgatcggt gcgggcctct tcgctattac gccagtcgat cgaccatagc caattcaata 3181 tggcgtatat ggactcatgc caattcaata tggtggatct ggacctgtgc caattcaata 3241 tggcgtatat ggactcgtgc caattcaata tggtggatct ggaccccagc caattcaata 3301 tggcggactt ggcaccatgc caattcaata tggcggactt ggcactgtgc caactgggga 3361 ggggtctact tggcacggtg ccaagtttga ggaggggtct tggccctgtg ccaagtccgc 3421 catattgaat tggcatggtg ccaataatgg cggccatatt ggctatatgc caggatcaat 3481 atataggcaa tatccaatat ggccctatgc caatatggct attggccagg ttcaatacta 3541 tgtattggcc ctatgccata tagtattcca tatatgggtt ttcctattga cgtagatagc 3601 ccctcccaat gggcggtccc atataccata tatggggctt cctaataccg cccatagcca 3661 ctcccccatt gacgtcaatg gtctctatat atggtctttc ctattgacgt catatgggcg 3721 gtcctattga cgtatatggc gcctccccca ttgacgtcaa ttacggtaaa tggcccgcct 3781 ggctcaatgc ccattgacgt caataggacc acccaccatt gacgtcaatg ggatggctca 3841 ttgcccattc atatccgttc tcacgccccc tattgacgtc aatgacggta aatggcccac 3901 ttggcagtac atcaatatct attaatagta acttggcaag tacattacta ttggaagtac 3961 gccagggtac attggcagta ctcccattga cgtcaatggc ggtaaatggc ccgcgatggc 4021 tgccaagtac atccccattg acgtcaatgg ggaggggcaa tgacgcaaat gggcgttcca 4081 ttgacgtaaa tgggcggtag gcgtgcctaa tgggaggtct atataagcaa tgctcgttta 4141 gggaac //