PCDH-CMV-mCherry-TRIM56

LOCUS       PCDH-CMV-mCherry-TRIM56              9420 bp DNA     circular SYN 04-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9420)
  AUTHORS   15129015045
  TITLE     Direct Submission
  JOURNAL   Exported Aug 11, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9420
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1076..1309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1798..1915
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     1904..1953
                     /label=Primer 4
     enhancer        1938..2241
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2242..2445
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2444..2491)
                     /label=Primer 1
     primer_bind     2466..2500
                     /label=Primer 2
     primer_bind     2466..2492
                     /label=Primer 5
     CDS             2496..3203
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     3025..3046
                     /label=mcherry-F
     misc_feature    3204..3232
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     3218..3254
                     /label=TRIM56-F
     primer_bind     3220..3243
                     /label=TRIM22-F
     misc_feature    3240..5507
                     /label=TRIM56
     primer_bind     3484..3501
                     /label=56-seqF1
     primer_bind     complement(3610..3625)
                     /label=56-seqR0
     CDS             complement(4249..4266)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     4829..4845
                     /label=56-seqF2
     primer_bind     complement(5424..5433)
                     /label=Primer 2
     primer_bind     complement(5490..5529)
                     /label=TRIM56-R
     primer_bind     complement(5504..5532)
                     /label=TRIM54-R
     primer_bind     complement(5505..5532)
                     /label=TRIM52-R
     misc_feature    5517..6104
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(5988..5999)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6177..6410
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6482..6603
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      6622..6757
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6795..6811)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6819..6835
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6843..6873)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6888..6909
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7197..7785)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7956..8816)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8143..8154)
                     /label=TRIM54-R
     promoter        complement(8817..8921)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9290..9303)
                     /label=Primer 5
     primer_bind     9395..9411
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagttgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     1981 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     2041 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     2101 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     2161 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     2221 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     2281 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     2341 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     2401 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
     2461 cagattctag accgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggaggata
     2521 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     2581 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     2641 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     2701 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     2761 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     2821 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     2881 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     2941 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     3001 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     3061 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     3121 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     3181 gcggcatgga cgagctgtac aagtccggac tcagatctcg agctcaagct tcggaattca
     3241 tggtttccca cgggtcctcg ccctccctcc tggaggccct gagcagcgac ttcctggcct
     3301 gtaaaatctg cctggagcag ctgcgggcac ccaagacact gccctgcctg catacctact
     3361 gccaagactg cctggcacag ctggcggatg gcggccgcgt ccgctgcccc gagtgccgcg
     3421 agacagtgcc tgtgccgccc gagggtgtgg cctccttcaa gaccaacttc ttcgtcaatg
     3481 ggctgctgga cctggtgaag gcccgggcct gtggagacct gcgtgccggg aagccagcct
     3541 gtgccctgtg tcccctggtg ggtggcacca gcaccggggg gccggccacg gcccggtgcc
     3601 tggactgtgc cgatgacttg tgccaggcct gtgccgacgg gcaccgctgc acccgccaga
     3661 cccacaccca ccgcgtggtg gacctggtgg gctacagggc cgggtggtat gatgaggagg
     3721 cccgggagcg ccaagcggcc cagtgtcccc agcaccccgg ggaggcactg cgcttcctgt
     3781 gccagccctg ctcacagttg ctgtgcagag agtgccgcct agacccccac ctggaccacc
     3841 cctgcctgcc tctggctgaa gctgtgcgtg cccggaggcc gggcctggag ggactgctgg
     3901 ccggtgtgga caataacctg gtggagctgg aggcagcgcg gagggtggag aaggaggcgc
     3961 tagcccggct gcgggagcag gcggcccggg tggggactca ggtggaggag gcggctgagg
     4021 gcgtcctccg ggccctgctg gcccagaagc aggaggtgct ggggcagcta cgagcccacg
     4081 tggaggctgc cgaagaagct gctcgggaga ggctggcgga gcttgagggc cgggagcagg
     4141 tggccagggc cgcagccgcc ttcgcccgcc gggtactcag cctggggcga gaggccgaga
     4201 tcctctccct ggaaggggcg atcgcacagc ggctcaggca gctgcagggc tgcccctggg
     4261 caccaggccc ggccccctgc ctgctcccac agctggagct ccatcctggg ctcctggaca
     4321 agaactgcca ccttcttcgg ctgtcctttg aggagcagca gccccagaag gatggtggga
     4381 aagacggagc tggtacccag ggaggtgagg agagccagag ccggagggag gatgagccga
     4441 agactgagag acagggtgga gtccagcccc aggctggaga tggagcccag accccaaaag
     4501 aggaaaaagc ccagacaacc cgagaagagg gagcccagac cttggaggag gacagggccc
     4561 agacacccca cgaggatgga ggaccccagc cccacagggg tggcagaccc aacaagaaga
     4621 aaaagttcaa aggcaggctc aagtcaattt cccgggagcc cagcccagcc ctggggccga
     4681 atctggacgg ctctggcctc ctccccagac ccatctttta ctgcagtttc cccacgcgga
     4741 tgcctggaga caagcggtcc ccccggatca ccgggctctg tcccttcggt ccccgggaga
     4801 tcctggtggc ggatgagcag aaccgggcac tgaaacgctt ctccctcaac ggcgactaca
     4861 agggcaccgt gccggtccct gagggctgct ccccttgcag cgtggccgcc ctgcagagcg
     4921 cggtggcctt ctccgctagc gcacggctct atctcatcaa ccccaacggc gaagtgcagt
     4981 ggcgcagggc cctgagcctc tcccaggcca gccacgcggt ggcggcactg cctagcgggg
     5041 accgcgtggc tgtcagcgtg gcgggccacg tggaggtgta caatatggaa ggcagcctgg
     5101 ccacccggtt cattcctgga ggcaaggcca gccggggcct gcgggcgctg gtgtttctga
     5161 ccaccagccc ccaggggcat ttcgtggggt cggactggca gcagaatagt gtggtaatct
     5221 gtgatgggct gggccaggtg gttggggagt acaaggggcc aggcctgcat ggctgccagc
     5281 cgggctccgt gtctgtggat aagaagggct acatctttct gacccttcga gaagtcaaca
     5341 aggtggtgat cctggacccg aaggggtccc tccttggaga cttcctgaca gcctaccacg
     5401 gcctggaaaa gccccgggtt accaccatgg tggatggcag gtacctggtc gtgtccctca
     5461 gtaacgggac catccacatc tttcgggtcc gttctccgga cagttaatag ggatccaatc
     5521 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     5581 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     5641 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     5701 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     5761 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     5821 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     5881 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     5941 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     6001 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtctccgcc
     6061 ttcgccctca gacgagtcgg atctcccttt ggccgcctcc ccgccggtac ctttaagacc
     6121 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     6181 agggctaatt cactcccaac gaaaataaga tctgcttttt gcttgtactg ggtctctctg
     6241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     6301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     6361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     6421 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     6481 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     6541 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     6601 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccagttccgc ccattctccg
     6661 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     6721 ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag agacggccca
     6781 aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     6841 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     6901 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     6961 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     7021 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     7081 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     7141 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     7201 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     7261 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     7321 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     7381 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     7441 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     7501 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     7561 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7621 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     7681 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     7741 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     7801 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     7861 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     7921 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     7981 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     8041 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     8101 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     8161 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     8221 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     8281 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     8341 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     8401 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     8461 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     8521 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     8581 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     8641 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     8701 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     8761 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     8821 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     8881 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     8941 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     9001 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     9061 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     9121 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     9181 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     9241 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     9301 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     9361 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//