| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-TCF20-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001417 |
| 产品用途: | 3' AAVLINK plasmid for TCF20 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | TCF20 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-TCF20-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-TCF20-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-TCF20-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-TCF20-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-TCF20-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-TCF20-FLAG 7027 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7027)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 6, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7027
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3729
/codon_start=1
/label=TCF20
/translation="VWAAP*D*WTWTS*GYTVIQTW*CYAETSRPGGSFFSKPLNHEEA
CSFFYLSHSQ*ETVTRCKEQ*H*R*RSPPSLIKRRR**SIQFLCPSFSQSGYQVYP*ER
FLQGPSKSR**KLPCCYPHKPC*DQNTAPTERTGIEIGSYSSEDYIPKY*EERIFEQCG
GWGRHGYA**YTVFEEWSS*RWECCCSGC*HREEKR*GGFGPSQSSKPGVARRETSSKV
FRRVAWQRG*QSEDRDTCRNSYCRKGTPWCHDIHNLTEAW**PRETRWFPGWNSTFNLS
RLKECTSSGHIGP*GKPQG*REGERYSDDFTEARGFPSKGIFPIRKEEGETHW*CE*AK
ETAAATASTPSAPTDTRRFCRWRAKAKKTEAKEGEKEAWGPAEEAKNQTSSSHCGTPRT
*DQTKICHPATG*N*CQEQVFLPLHPCSK*V*TWSRLYNHQC*GRRTDQISEGQEGSEV
TDPST*QH*KQGAPGLVLYAAGTCCDRVFGYGAPGLLSVWQVGQLPEHG*PLWTFLSPR
LCSHSPEESTS*EGHRNAEQS*GTAQKCF*WLQDGH*GGGRAAAAAEGAEKPGRTPQV*
AAPPLGRLWWRPSVPVQGAPL*KSSH*GQQ*KDCFGLEALRAHHFRRWP*AGVTNP*TT
S*QQ*ILGP*GLYSLGQWNLPGLWQALWPAGSAGNSQRDEMFPLPGGRRHLGLLQQRLL
LPIPLPVCH*CRLFAT*GELLGEVP*AQASPSVPSPPLAEQDRERQPQHRAVGAG"
misc_feature 1574..1579
/label=SDS
misc_feature 1609..1614
/label=SDS
misc_feature 1698..1703
/label=SDS
misc_feature 1942..1947
/label=SDS
misc_feature 2023..2028
/label=SDS
misc_feature 2596..2601
/label=SDS
misc_feature 3061..3066
/label=SDS
misc_feature 3143..3148
/label=SDS
misc_feature 3146..3151
/label=SDS
misc_feature 3521..3526
/label=SDS
CDS 3736..3801
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3817..4247
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3961..3966
/label=SDS
repeat_region 4290..4430
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4290..4419
/label=AAV2 ITR
rep_origin 4505..4960
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5242..5346
/gene="bla"
/label=AmpR promoter
CDS 5347..6207
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6378..6966
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtatgggcc gccccatgag
1501 actgatggac atggactagc tgaggctaca cagtcatcca aacctggtag tgttatgctg
1561 agacttccag gccaggagga tcattcttct caaaacccct taatcatgag gaggcgtgtt
1621 cgttctttta tctctcccat tcccagtaag agacagtcac aagatgtaaa gaacagtagc
1681 actgaagata aaggtcgcct ccttcactca tcaaaagaag gcgctgataa agcattcaat
1741 tcctatgccc atctttctca cagtcaggat atcaagtcta tccctaagag agattcctcc
1801 aaggaccttc caagtccaga tagtagaaac tgccctgctg ttaccctcac aagccctgct
1861 aagaccaaaa tactgccccc acggaaagga cggggattga aattggaagc tatagttcag
1921 aagattacat ccccaaatat taggaggagc gcatcttcga acagtgcgga ggctggggga
1981 gacacggtta cgcttgatga tatactgtct ttgaagagtg gtcctcctga aggtgggagt
2041 gttgctgttc aggatgctga catagagaag agaaaaggtg aggtggcttc ggacctagtc
2101 agtccagcaa accaggagtt gcacgtagag aaacctcttc caaggtcttc agaagagtgg
2161 cgtggcagcg tggatgacaa agtgaagaca gagacacatg cagaaacagt tactgccgga
2221 aaggaacccc ctggtgccat gacatccaca acctcacaga agcctggtag taaccaaggg
2281 agaccagatg gttccctggg tggaacagca cctttaatct ttccagactc aaagaatgta
2341 cctccagtgg gcatattggc ccctgaggca aaccccaagg ctgaagagaa ggagaacgat
2401 acagtgacga tttcaccgaa gcaagagggt ttccctccaa agggatattt cccatcagga
2461 aagaagaagg ggagacccat tggtagtgtg aataagcaaa agaaacagca gcagccaccg
2521 cctccacccc ctcagccccc acagatacca gaaggttctg cagatggaga gccaaagcca
2581 aaaaaacaga ggcaaaggag ggagagaagg aagcctgggg cccagccgag gaagcgaaaa
2641 accaaacaag cagttcccat tgtggaaccc caagaacctg agatcaaact aaaatatgcc
2701 acccagccac tggataaaac tgatgccaag aacaagtctt tttaccctta catccatgta
2761 gtaaataagt gtgaacttgg agccgtttgt acaatcatca atgctgagga agaagaacag
2821 accaaattag tgaggggcag gaagggtcag aggtcactga cccctccacc tagcagcact
2881 gaaagcaagg cgctcccggc ctcgtccttt atgctgcagg gacctgttgt gacagagtct
2941 tcggttatgg ggcacctggt ttgctgtctg tgtggcaagt gggccagtta ccggaacatg
3001 ggtgacctct ttggaccttt ttatccccaa gattatgcag ccactctccc gaagaatcca
3061 cctcctaaga gggccacaga aatgcagagc aaagttaagg tacggcacaa aagtgcttct
3121 aatggctcca agacggacac tgaggaggag gaagagcagc agcagcagca gaaggagcag
3181 agaagcctgg ccgcacaccc caggtttaag cggcgccacc gctcggaaga ctgtggtgga
3241 ggccctcggt ccctgtccag ggggctccct tgtaaaaaag cagccactga gggcagcagt
3301 gaaaagactg ttttggactc gaagccctcc gtgcccacca cttcagaagg tggccctgag
3361 ctggagttac aaatccctga actacctctt gacagcaatg aattttgggt ccatgagggt
3421 tgtattctct gggccaatgg aatctacctg gtttgtggca ggctctatgg cctgcaggaa
3481 gcgctggaaa tagccagaga gatgaaatgt tcccactgcc aggaggcagg cgccaccttg
3541 ggctgctaca acaaaggctg ctccttccga taccattacc cgtgtgccat tgatgcagat
3601 tgtttgctac atgaggagaa cttctcggtg aggtgcccta agcacaagcc tccccttccg
3661 tgccctctcc cccccttgca gaacaagacc gcgaaaggca gcctcagcac agagcagtcg
3721 gagcgggggt gtacagacta caaagaccat gacggtgatt ataaagatca tgacatcgac
3781 tacaaggatg acgatgacaa gtaagaattc tgatgactcg agataatcaa cctctggatt
3841 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
3901 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
3961 cctccttgta taaatcctgg ttagttcttg ccacggcgga actcatcgcc gcctgccttg
4021 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg tttatttgtg
4081 aaatttgtga tgctattgct ttatttgtaa ccatctagct ttatttgtga aatttgtgat
4141 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
4201 attcatttta tgttcaggtt cagggggaga tgtgggaggt tttttaaaga tctggagacc
4261 ggtgtggcac gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc
4321 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
4381 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat
4441 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca
4501 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
4561 accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc
4621 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
4681 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
4741 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
4801 agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat
4861 ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa
4921 tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca
4981 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
5041 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
5101 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
5161 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
5221 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
5281 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
5341 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
5401 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
5461 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
5521 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
5581 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
5641 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
5701 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
5761 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
5821 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
5881 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
5941 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
6001 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
6061 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
6121 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
6181 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
6241 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
6301 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
6361 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
6421 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
6481 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
6541 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
6601 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
6661 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
6721 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
6781 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
6841 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
6901 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
6961 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
7021 cacatgt
//
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