| 质粒名称: | pmEGFP-TPH2(human)-TEV-Twinstrep |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002717 |
| 产品用途: | Mammalian expression of human TPH2 (tryptophan hydroxylase 2) as a single ORF fusion: TPH2-TEV(ENLYFQ)-Twin-Strep(WSHPQFEK)-mEGFP. CMV promoter; non-lentiviral vector; KanR (bacterial) / Neo-G418 (mammalian) selection. Two synonymous mutations as noted. RefSeq NM_173353.4; aliases ADHD7, NTPH. |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pmEGFP-TS |
| 表达系统: | 哺乳动物表达 |
| 启动子: |
CMV
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | TPH2 |
| 物种来源: | Human |
| 密码子优化: | 大肠杆菌表达优化 |
pmEGFP-TPH2(human)-TEV-Twinstrep
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pmEGFP-TPH2(human)-TEV-Twinstrep载体质粒基本信息
pmEGFP-TPH2(human)-TEV-Twinstrep质粒图谱载体图谱和pmEGFP-TPH2(human)-TEV-Twinstrep载体序列质粒序列多克隆位点信息
-TEV-Twinstrep载体图谱.png)
pmEGFP-TPH2(human)-TEV-Twinstrep质粒载体简介
pmEGFP-TPH2(human)-TEV-Twinstrep质粒序列载体序列
LOCUS pmEGFP-TPH2(human)-TEV-Twinstrep 6293 bp DNA circular SYN 18-AUG-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6293)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Sep 14, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6293
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 2101..2121
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
/label=TEV site
/translation="ENLYFQG"
CDS 2134..2217
/codon_start=1
/product="two Strep-Tag(R)II moieties connected by a linker
for enhanced binding to Strep-Tactin(R), an engineered form
of streptavidin (Schmidt et al., 2013)"
/label=Twin-Strep-tag
/translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
regulatory 2233..2242
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 2239..2958
/codon_start=1
/product="enhanced GFP with monomerizing A206K mutation
(Zacharias et al., 2002)"
/label=mEGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
polyA_signal 3081..3202
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(3209..3664)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3691..3795
/gene="bla"
/label=AmpR promoter
promoter 3797..4154
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 4189..4983
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 5215..5262
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 5591..6179
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggactcag atctcgaggc caccatgcag ccagcaatga tgatgttttc cagtaaatac
661 tgggcacgga gagggttttc cctggattca gcagtgcccg aagagcatca gctacttggc
721 agctcaacac taaataaacc taactctggc aaaaatgacg acaaaggcaa caagggaagc
781 agcaaacgtg aagctgctac cgaaagtggc aagacagcag ttgttttctc cttgaagaat
841 gaagttggtg gattggtaaa agcactgagg ctctttcagg aaaaacgtgt caacatggtt
901 catattgaat ccaggaaatc tcggcgaaga agttctgagg ttgaaatctt tgtggactgt
961 gagtgtggga aaacagaatt caatgagctc attcagttgc tgaaatttca aaccactatt
1021 gtgacgctga atcctccaga gaacatttgg acagaggaag aagagctaga ggatgtgccc
1081 tggttccctc ggaagatctc tgagttagac aaatgctctc acagagttct catgtatggt
1141 tctgagcttg atgctgacca cccaggattt aaggacaatg tctatcgaca gagaagaaag
1201 tattttgtgg atgtggccat gggttataaa tatggtcagc ccattcccag ggtggagtat
1261 actgaagaag aaactaaaac ttggggtgtt gtattccggg agctctccaa actctatccc
1321 actcatgctt gccgagagta tttgaaaaac ttccctctgc tgactaaata ctgtggctac
1381 agagaggaca atgtgcctca actcgaagat gtctccatgt ttctgaaaga aaggtctggc
1441 ttcacggtga ggccggtggc tggatacctg agcccacgag actttctggc aggactggcc
1501 tacagagtgt tccactgtac ccagtacatc cggcatggct cagatcccct ctacaccccg
1561 gaaccagaca catgccatga actcttggga catgttccac tacttgcgga tcctaagttt
1621 gctcagtttt cacaagaaat aggtctggcg tctctgggag catcagatga agatgttcag
1681 aaactagcca cgtgctattt cttcacaatc gagtttggcc tttgcaagca agaagggcaa
1741 ctgcgggctt atggagcagg actcctttcc tccattggag aattaaagca cgccctttct
1801 gacaaggcat gtgtgaaagc ctttgaccca aagacaactt gcttacagga atgccttatc
1861 accaccttcc aggaagccta ctttgtttca gaaagttttg aagaagccaa agaaaagatg
1921 agggactttg caaagtcaat tacccgtccc ttctcagtat acttcaatcc ctacacacag
1981 agtattgaaa ttctgaaaga caccagaagt attgaaaatg tggtgcagga ccttcgcagc
2041 gacttgaata cagtgtgtga tgctttaaac aaaatgaacc aatatctggg gattggtacc
2101 gagaatctct acttccaggg acatatggct agctggagcc atccgcagtt tgaaaaaggt
2161 ggtggtagcg gtggtggttc aggtggtagt gcatggtcac accctcagtt tgagaaactg
2221 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
2281 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
2341 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
2401 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
2461 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
2521 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
2581 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
2641 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
2701 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
2761 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
2821 ctgctgcccg acaaccacta cctgagcacc cagtccaagc tgagcaaaga ccccaacgag
2881 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
2941 gacgagctgt acaagtaaag cggccgcgac tctagatcat aatcagccat accacatttg
3001 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
3061 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
3121 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
3181 ccaaactcat caatgtatct taaggcgtaa attgtaagcg ttaatatttt gttaaaattc
3241 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
3301 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
3361 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
3421 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
3481 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
3541 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
3601 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
3661 gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
3721 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
3781 tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt
3841 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
3901 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
3961 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
4021 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
4081 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
4141 taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
4201 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
4261 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
4321 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca
4381 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
4441 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
4501 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
4561 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
4621 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
4681 ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc
4741 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
4801 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
4861 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
4921 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
4981 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
5041 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
5101 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg
5161 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa
5221 taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt
5281 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg
5341 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca
5401 acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt tagattgatt
5461 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
5521 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
5581 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
5641 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
5701 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
5761 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
5821 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
5881 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
5941 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
6001 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
6061 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
6121 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
6181 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
6241 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg cat
//
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