| 质粒名称: | pSL185 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002419 |
| 产品用途: | GAL4-specific SEAP expression vector ((UAS)5-Pmin-SEAP-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | UAS5-Pmin |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | SEAP |
| 物种来源: | synthetic |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Rationally designed light-inducible RNA-releasing protein for translational regulation and optogenetic control of gene therapies
NA
NA
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pSL185
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pSL185载体质粒基本信息
pSL185质粒图谱载体图谱和pSL185载体序列质粒序列多克隆位点信息
pSL185质粒载体简介
pSL185质粒序列载体序列
LOCUS pSL185 6401 bp DNA circular SYN 05-MAR-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6401)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 28, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6401
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 214..232
/label=oSCL1980
misc_feature 228
/label=E2F-1
misc_feature 234..328
/label=5*UAS
promoter 356..387
/label=minP
/note="minimal TATA-box promoter with low basal activity"
misc_feature 405..428
/label=New Feature
primer_bind complement(405..428)
/label=oSCL1548
primer_bind 429..455
/label=Oscl13-1
misc_feature 430..1950
/label=SEAP
primer_bind complement(1930..1958)
/label=Oscl23-2
primer_bind complement(1932..1950)
/label=Oscl13-2
primer_bind complement(1932..1950)
/label=Primer 2
terminator 1998..2225
/label=bGH PA
rep_origin 2271..2699
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2713..3042
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 3109..3903
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 4077..4198
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4243..4263)
/label=M13-rev
misc_binding complement(4269..4291)
/label=LacO
promoter complement(4295..4325)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4340..4361
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4649..5234)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5405..6265)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6266..6370)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtcggagta
241 ctgtcctccg agcggagtac tgtcctccga gcggagtact gtcctccgag cggagtactg
301 tcctccgagc ggagtactgt cctccgagac tagactcggc ggcctactta gacactagag
361 ggtatataat ggaagctcga cttccagaaa ttcgagctcg cccggggatc ctctagagtc
421 agcttctgca tgctgctgct gctgctgctg ctgggcctga ggctacagct ctccctgggc
481 atcatcccag ttgaggagga gaacccggac ttctggaacc gcgaggcagc cgaggccctg
541 ggtgccgcca agaagctgca gcctgcacag acagccgcca agaacctcat catcttcctg
601 ggcgatggga tgggggtgtc tacggtgaca gctgccagga tcctaaaagg gcagaagaag
661 gacaaactgg ggcctgagat acccctggcc atggaccgct tcccatatgt ggctctgtcc
721 aagacataca atgtagacaa acatgtgcca gacagtggag ccacagccac ggcctacctg
781 tgcggggtca agggcaactt ccagaccatt ggcttgagtg cagccgcccg ctttaaccag
841 tgcaacacga cacgcggcaa cgaggtcatc tccgtgatga atcgggccaa gaaagcaggg
901 aagtcagtgg gagtggtaac caccacacga gtgcagcacg cctcgccagc cggcacctac
961 gcccacacgg tgaaccgcaa ctggtactcg gacgccgacg tgcctgcctc ggcccgccag
1021 gaggggtgcc aggacatcgc tacgcagctc atctccaaca tggacattga cgtgatccta
1081 ggtggaggcc gaaagtacat gtttcccatg ggaaccccag accctgagta cccagatgac
1141 tacagccaag gtgggaccag gctggacggg aagaatctgg tgcaggaatg gctggcgaag
1201 cgccagggtg cccggtatgt gtggaaccgc actgagctca tgcaggcttc cctggacccg
1261 tctgtgaccc atctcatggg tctctttgag cctggagaca tgaaatacga gatccaccga
1321 gactccacac tggacccctc cctgatggag atgacagagg ctgccctgcg cctgctgagc
1381 aggaaccccc gcggcttctt cctcttcgtg gagggtggtc gcatcgacca tggtcatcat
1441 gaaagcaggg cttaccgggc actgactgag acgatcatgt tcgacgacgc cattgagagg
1501 gcgggccagc tcaccagcga ggaggacacg ctgagcctcg tcactgccga ccactcccac
1561 gtcttctcct tcggaggcta ccccctgcga gggagctcca tcttcgggct ggcccctggc
1621 aaggcccggg acaggaaggc ctacacggtc ctcctatacg gaaacggtcc aggctatgtg
1681 ctcaaggacg gcgcccggcc ggatgttacc gagagcgaga gcgggagccc cgagtatcgg
1741 cagcagtcag cagtgcccct ggacgaagag acccacgcag gcgaggacgt ggcggtgttc
1801 gcgcgcggcc cgcaggcgca cctggttcac ggcgtgcagg agcagacctt catagcgcac
1861 gtcatggcct tcgccgcctg cctggagccc tacaccgcct gcgacctggc gccccccgcc
1921 ggcaccaccg acgccgcgca cccgggttaa gcggccgctc gagtctagag ggcccgttta
1981 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
2041 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
2101 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
2161 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
2221 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
2281 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
2341 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
2401 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
2461 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
2521 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
2581 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
2641 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
2701 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
2761 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
2821 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
2881 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
2941 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
3001 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
3061 atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
3121 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
3181 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
3241 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
3301 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
3361 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
3421 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
3481 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
3541 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
3601 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
3661 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
3721 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
3781 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
3841 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
3901 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
3961 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
4021 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
4081 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
4141 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
4201 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
4261 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
4321 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
4381 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
4441 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
4501 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
4561 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
4621 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
4681 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
4741 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
4801 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
4861 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
4921 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
4981 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
5041 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
5101 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
5161 gcaaacaaac caccgctggt agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa
5221 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
5281 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
5341 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
5401 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
5461 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
5521 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
5581 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
5641 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
5701 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
5761 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
5821 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
5881 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
5941 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
6001 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
6061 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
6121 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
6181 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
6241 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
6301 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
6361 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt c
//
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