pYSJ37

LOCUS       pYSJ37               7379 bp DNA     circular SYN 14-DEC-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 7379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 20, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7379
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     914..954
                     /label=oYSJ121-mELF4-F
     misc_feature    935..2902
                     /label=Mus ELF4
     primer_bind     complement(2868..2923)
                     /label=oYSJ122-mELF4-R
     polyA_signal    2979..3203
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3249..3677
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3691..4020
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3871..4006
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4087..4881
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    5055..5176
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5225..5241)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5249..5265
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5273..5303)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5318..5339
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(5627..6212)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6383..7243)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7244..7348)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccatggct attgctctgc agcccagtga
      961 tctggtcttc gagtttgcaa gcaacgggat ggatgatatc caccagctgg aagacccttc
     1021 agtgttccca gccgtgatcg tggaacaggt cccctatcct gaattagtgc atctgtgctc
     1081 aggactggat ctggatgagg tccacaatgg catcataagg gacaggacct tgtgcatgac
     1141 acaggatcag attttagaag gcagtatttt gctgacagat gatgatgtat ccacctcaaa
     1201 taacgtgtca tccactgaag tcctattcaa tgtggcaact cccagtgatg tcctggatga
     1261 gaagcagatc ttcagctcac cggaagtgct ttcagactcc aactctgttc aggccatcaa
     1321 tctacccaac ttcctgctct ctacccctga acctgatgac ctgaagaaga caagtgatgc
     1381 tggtgaccag aaggagcatt ctgaggagga gaaggtctcc agagaggaaa atcttaggaa
     1441 gatggggaag gcacggaaga gaaaccgaaa gaccaagaac aaccgaagta cctcacctgt
     1501 cactgaccct agcatgccta tacggaagaa atccaaagat ggcaaaggca gcaccattta
     1561 tctgtgggag ttcctcctgg ctcttctgca agacagaaac acctgcccca agtacattaa
     1621 gtggacgcag agagagaagg gcatcttcaa gttggtggac tccaaggctg tgtccaagct
     1681 gtggggcaag cagaaaaaca aacctgacat gaactacgag accatggggc gagccctgag
     1741 atattattat caaagaggaa tcctggccaa agtggaaggg cagaggctgg tataccagtt
     1801 caaggagatg cctaaggact tggtggtgat tgatgatgaa gaagagagcc ctgagacacc
     1861 agaagactct tcccaggcct cgacttcctc cacaccctcg accagtacca tccggagagc
     1921 cagctccagg gtcggaacca gagcctcccc cgaggacaag gataaccctc cctgggagaa
     1981 gccaaaagtc caacacactg gtctccagcc atctgcaagc ctggagttag gattgtctgt
     2041 ggatgaggag gttcccacca cctccaccat gcttgccagc ccactacaga gccaggccag
     2101 gctcaccaaa actgtgagtt cttctccagc acccagtaac atccacctag gagtagcccc
     2161 tgtggggcct ggctccactg tcaccctgca gaccatcccg ctgactacgg tgctgaccaa
     2221 tgggcctcct gccagtacta ctgctccaac tcaactcgtt cttcagagtg tcccgcaggt
     2281 gtcaacattc aaggacacct tcactttgca gacctccttc cccctgaaca ccaatttgca
     2341 agagaaccag gtggcaaccc aaggggcccc actgatcctc agtggtctcc cccaacttct
     2401 ggctggggcc aaccctcaga gcaacccagc tccatcccag gtcataggtg ctgggtcagc
     2461 agggcctagc tctcagcccc ctgggactgt gattgctgcc tttatcagga cttccagtgg
     2521 cacatcagtg cctgtggtaa aggaggggcc cttgaggtcc tcttcatatg tgcagggtgt
     2581 ggttactggg gcacccgtgg aggggctgtt ggttcctgaa gagaccctga gagagctcct
     2641 gagagatcag ggtcatcttc agccacttcc gagtcaggtg ctctcaaggg gttcccataa
     2701 cctgagcctt gtggggaacc agactctctc tccccctagc caccccactg ttgggctaac
     2761 cccagtggct gagctcgagc tctcgtcagg ctcagggccc ctgttcgtaa ctgagcctag
     2821 tgtgaccaga tccccaaccc aggccccttt ctccccattc aatcccactt ccctcattaa
     2881 gatggaaccc caagatatat aagaattctg cagatatcca gcacagtggc ggccgctcga
     2941 gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     3001 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     3061 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     3121 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     3181 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     3241 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     3301 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     3361 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     3421 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     3481 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     3541 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     3601 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     3661 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     3721 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     3781 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     3841 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     3901 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
     3961 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     4021 aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg
     4081 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     4141 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     4201 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     4261 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     4321 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     4381 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     4441 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     4501 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     4561 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
     4621 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     4681 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     4741 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     4801 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     4861 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
     4921 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     4981 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     5041 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     5101 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     5161 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     5221 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     5281 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     5341 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     5401 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     5461 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     5521 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     5581 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     5641 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     5701 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     5761 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     5821 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     5881 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     5941 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     6001 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     6061 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     6121 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggttttttt gtttgcaagc
     6181 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     6241 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     6301 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     6361 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     6421 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     6481 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     6541 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     6601 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     6661 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     6721 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     6781 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     6841 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     6901 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     6961 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     7021 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     7081 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     7141 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     7201 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     7261 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     7321 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc
//